You plan to clone a guide RNA sequence into the pML104 plasmid because you want to engineer the construct in bacteria and later express it in yeast. Unfortunately, your lab does not have any available pML104. Your friend in the lab next door lets you borrow a related plasmid pML107, and you quickly start your experiment. Will it work? Briefly explain.

Answers

Answer 1

Answer:

Yes, the experiment will work.

Explanation:

The reason behind this is that plasmids are well known to be DNA vector carriers. These plasmids have the ability to bring about changes in DNA fragments into various microorganisms and reveal them. The two plasmids compose of the same genomic marker such as the antibiotic resistance gene found in URA3. However, plasmids are most commonly found in bacteria, but also in eukaryotic cells. Oftentimes, the gene composition in the plasmids enhances the bacteria with a vantage edge such as antibiotic resistance.

Hence, it is obvious that in both, the molecular regulation in terms of expression of genes is alike in properties. Similarly, the numbers of copies and molecular weight are also the same.

We can thereby conclude that, provided that there is a proper restriction site, another host that is up to standard for plasmid can be replaced for each other.


Related Questions

Write each phrase into the appropriate category.
a. cross between organisms that are the same for two traits
b. the cross was performed by Mendel using pure-breeding lines
c. cross between organisms that differ for three traits
d. cross between organisms that are the same for three traits
e. cross between organisms that differ for two traits
dihybrid cross trihybrid cross both none

Answers

Answer:

a. none

b dihybrid and trihybrid crosses (both)

c. trihybrid cross

d. none

e. dihybrid cross

Explanation:

A dihybrid cross can be defined as a mating experiment between two different varieties/lines that differ in two observed phenotypic traits, while a trihybrid cross is a cross between two varieties/lines that differ in three traits. For example, Mendel crossed two pea plants that differed in seed color (yellow and green) and seed shape (wrinkled and round). Mendel's experiments consisted of dihybrid and trihybrid crosses for different phenotypic traits. From these mating experiments, he observed dihybrid F1 plants produced 9:3:3:1 ratio of phenotypes in the F2 generation.

39. Which macromolecule is represented below.
a.lipid
b. protein
C. nucleic acid
d. carbohydrate

Answers

Answer:

C is the correct answer.

Explanation:

i attached a photo of the structure of two different nucleic acids. the structure shown on your paper matches up with the DNA model, therefore c. nucleic acid is the correct answer.

Which characteristics do many protest share with bacteria and archae

Answers

Answer:

They are microbes and prokaryotes (no nucleus and they have a lack of membrane-bound organelles). They can be unicellular or multicellular (mostly unicellular)

Hope this helped!

Have a supercalifragilisticexpialidocious day!

the diagram shows a bacterium. Which labels best complete the diagram

Answers

Answer:

X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm

Explanation:

A bacterium is a living thing that are majorly known for causing diseases but are important for human life.

A bacterium is also consist of several organelles such as cell wall, cytoplasm, flagellum,  bacterial DNA, cytoplasm, ribosomes and others.

The diagram shows three main organelles that are X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm. Flagella functions for the locomotion of the bacteria, bacterial DNA is a genetic material of the bacteria, and cytoplasm is a gel-like matrix composed of enzymes, water, nutrients and other waste material.

Hence, the correct answer is "X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm".

How many factors does a scientist want to differ between the experimental and control groups?

A) 2

B) 0

C) 1

D) 3

Answers

Answer:

C

Explanation:

The experimental and control group differ by only one factor for a scientist, and that is the independent variable.

A scientist will always try to isolate the effects that are due to a variable and keep every other variable constant. The variable that is manipulated in order to measure its effect is known as the independent variable. A control group usually forms the basis for measuring the effects of the independent variable. While the independent variable is varied in experimental groups, it kept at the zero level for the control group.

The correct option is, therefore, C.

List the three pairs of bones that make up the pelvis in the adult animal

Answers

Answer:

the ilium, ischium, and pubis.

Explanation:

In adults, the hip bones, or coxal bones are formed by the fusion of three pairs of bones: the ilium, ischium, and pubis.

Norman grew skin in a lab by adding skin cells to a synthetic material. The skin functioned normally for 12 days. Then Norman separated some of the skin cells into cell membranes, cytoplasm, and vacuoles to study them.
Which of the following were alive during Norman's experiment?
o the skin and the cytoplasm
o the skin and the skin cells
o the cell membranes and the skin cells
O the cell membranes and the cytoplasm

Answers

Answer:

D

Explanation:

Answer: D:the cell membranes and cytoplasm

Explanation:

i took the test and got it right

Mutations result in changes to genotype that can result in changes in phenotype. Explain how a mutation causes a change in the genotype of an organism AND may result in a change in the phenotype of an organism.

Answers

Answer:

A mutation can alter the structure/function of a particular protein, thereby also altering the phenotype resulting from this new variant

Explanation:

A mutation can be defined as a genetic change in the genome of an organism. Some mutations are capable of modifying the expression and/or structure of the proteins, while other mutations (known as silent mutations) have no effect on the resulting proteins. When mutations occur within the gene region encoding a protein (i.e., exons), they are potentially capable of producing a faulty protein. For example, a mutation can alter the Open Reading Frame (ORF) of the resulting protein, thereby inactivating it. The mutations that alter the structure and/or function of the protein can also alter the resulting phenotype associated with the expression of this protein. For example, a mutation within a gene that encodes a key enzyme can potentially alter the binding site of the protein, so the resulting mutated enzyme cannot bind to the substrate anymore. In consequence, this mutation alters the phenotype of the individual who is not more able to carry out the metabolic reaction catalyzed by the faulty enzyme.

What is responsible for earths seasons?

Earths tilt

Closeness of the sun

Greenhouse effect

Ocean currents

Answers

Answer:

A. The Earth's tilt

Explanation:

Answer:

b

Explanation:

the earth roates around the sun but it does so more in an oval shape

the earthe to distance from the sun to change.

What gametes are produced by the following organisms:_______.
a. Genotype YyRr, if Y and R are on different chromosomes
b. Genotype YyRr, if dominant Y and R are on the same chromosome, no crossing over
c. Genotype YyRr, if dominant Y and recessive r are on the same chromosome, no crossing over
d. Genotype YyRr, if dominant Y and R are on the same chromosome, 50% crossing over

Answers

Answer:

The correct answer would be -

1- YR, Yr, yR and yr.

2. Yr, yR

3. YR, yr

4. YR, Yr, yR & yr

Explanation:

A mature male or female haploid germ cell that fuses with another gamete of the opposite sex during the process of fertilization to make a zygote in sexual reproduction.

The law of Independent Assortment presented by Mendel says that alleles for different traits are passed and assort independently of one another in the gametes.

1. If Y and R on different chromosomes then they will produce four gametes and assort independently - YR, Yr, yR & yr.

2) If Y and R on the same chromosomes and no crossing over occurs then they will produce only two gametes as Y and R will not come together - Yr & yR.

3).  if dominant Y and recessive r are on the same chromosome, no crossing over then only two gametes will form as Y and r will not assort together - YR & yr.

4). if dominant Y and R are on the same chromosome, 50% crossing over produces 4 types of gametes, YR, Yr, yR & yr.

The color of a light wave is determined by its wavelength. On the COLOR tab, slowly drag the Light wavelength slider back and forth and observe the effect on oxygen production. How does the color of light affect the rate of photosynthesis

Answers

Answer:

The color of light determines how effective chlorophyll pigment will be at absorbing light.

Explanation:

Photosynthesis is a metabolic process that requires light, which is an electromagnetic radiation. Light on the electromagnetic spectrum is determined by wavelength. Each region/color of light occurs at a specific wavelength.

Hence, chlorophyll pigment, which is the major photosynthetic pigment effectively absorbs blue and red light in the visible light region of the electromagnetic spectrum. Hence, the rate of photosynthesis will be affected negatively if the color of light is not the one stated above.

3. A population of 70 jaguars lives in a rainforest measuring 350 square miles. What is the population
density of the jaguars? (Hint: You cannot have a fraction of a jaguar)

Answers

Answer:

5 jaguars per square mile.

Explanation:

If you do 350 ÷ 70 it comes to 5, so that means it's 5 jaguars per square mile. Have a fun day. :)

The population density of jaguars in the rainforest is 0.2 jaguars per square mile.

To calculate the population density, we divide the total number of individuals (70 jaguars) by the area of the habitat (350 square miles).

Population density = Total number of individuals / Area

Population density = 70 jaguars / 350 square miles

Simplifying the calculation, we find that 70 divided by 350 equals 0.2. Therefore, the population density of jaguars in the rainforest is 0.2 jaguars per square mile.

Population density is a measure of how concentrated a population is within a given area. In this case, it tells us how many jaguars are found on average in each square mile of the rainforest. A population density of 0.2 jaguars per square mile means that, on average, there are 0.2 jaguars in every square mile of the rainforest.

It's important to note that population density can vary across different habitats and species. Factors such as food availability, territory size, and reproductive behavior can influence the distribution and concentration of individuals within a given area. Understanding population density helps researchers assess the health of a population, monitor changes over time, and make informed conservation and management decisions.

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Mendel used over 28,000 pea plants in his experiment. How does this large
sample size make his results more reliable?

Answers

Answer with Explanation:

The large sample size allowed Gregor Mendel to make comparisons quantitatively. The "margin of error" is narrower and it allowed him to detect statistically significant results through its statistical power. Large sample sizes have a greater statistical power. However, it takes a lot of time, effort and money. Mendel was very persistent and had the driving force. This allowed him to develop the "Three Principles of Inheritance."

A smaller sample size doesn't give a significant difference because the samples are quite random. This has a tendency to mask the result.

A disease infects an organism, causing the organism's cells to have less chloroplasts. How will the organism be impacted by having less chloroplasts?
The organism will have less chlorophy reducing its ability to collect energy
The organism will have less cellulose, reducing its ability to remove waste
The organism will absorb less oxygen, reducing its ability to produce sugar
The organism will absorb less water, reducing its ability to create DNA

Answers

Answer:

the organism will have less chlorophyll

Explanation:

chloroplasts contain chlorophyll

Chlorophyll is a green pigment, without it a plant is discolored meaning it lacks of chlorophyll. Less photosynthesis would occur, and less glucose is made as a result. There is less energy released for growth as glucose is needed for respiration.

paragraph-how does pollution affect ecologist job.

Answers

Answer:

pollution affects ecologist job by giving him something to work with

Explanation:

whether it be restoration or simply studying an ecosystem, however don't confuse this with benefitting him because he is probably not happy about it.

The principles of Mendelian genetics are grounded in mathematical reasoning. Mendel used mathematical analysis to explain the visible traits (phenotypes) and the genetic make-up (genotype) resulting from experimental crosses. Therefore, it is important for us to familiarize ourselves with concepts of percent deviation and sample size.

a. True
b. False

Answers

Answer:

True

Explanation:

According the Mendelian research, it is always good to work with large sample size because small sample size are prone to deviations. Small sample sizes easily get affected by genetic forces such as genetic drift, selection, immigration etc.

Hence the given statement is true

Which of the following is true according to the theory of plate tectonics?
Plate tectonics only apply to the Earth’s continental crust.
The Earth’s crust is one solid plate.
Earth’s mountains all formed when the crust first formed.
Mantle convection currents cause plate movement.

Answers

Answer:D

Explanation:Mantle convection currents cause plate movement

A characteristic of living organisms is their ability to maintain internal balance.
Maintaining internal balance inside the body is

Answers

Answer:

Homeostasis.

Explanation:

A characteristic of living organisms is their ability to maintain internal balance. Therefore, maintaining internal balance inside the body is known as homeostasis.

Homeostasis can be defined as a process through which a living organism maintains a steady or stable physical, internal and chemical environment ideal to enhance life and proper functionality.

For example, if a person becomes infected with a pathogen such as a virus. The internal stimulus that most likely takes place in order for their body to maintain homeostasis is that their core temperature increases. This is because pathogens such as a virus do not thrive or survive in a hot environment; they're naturally allergic to high temperature (heat).

Which best explains why water quality standards have been created to ensure heavy metals in water are kept to a minimum?
O A. Heavy metals are linked to toxicities in humans and large amounts of heavy metals should not be consumed.
OB. Heavy metals can stain ceramic surfaces in homes and cost the homeowners hundreds in repairs.
OC. Heavy metals can cause discoloration of water and can cause a strong unpleasant odor.
O D. Heavy metals can corrode pipes and cause problems with internal plumbing.

Answers

its OB because if you read it says it praticly

Heavy metals are harmful and should not be consumed in large amounts. Therefore, option (A) is correct.

What are harmful effects of heavy metal in water?

The flow of groundwater and surface water, as well as seepage and runoff from the surface, can introduce heavy metals into private wells. People who consume significant quantities of heavy metals put themselves at risk for acute and chronic toxicity, as well as anemia, cancer, and damage to their liver, kidneys, and intestines.

Heavy metals in water are usually treated using methods like chemical precipitation, flotation, adsorption, ion exchange, and electrochemical deposition. Most of the time, heavy metals are taken out of inorganic effluent by a process called chemical precipitation.

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The Nile hippopotamus has somatic cells with 36 chromosomes. How many chromosomes will their gametes have?

PLEASE HELP NOWWW

Answers

Answer:

18 chromosomes

Explanation:

if their somatic cell have 36 chromosomes then gamete have 18 chromosomes. because meiosis is a reduction division and produce gametes with half number of chromosomes. so their gametes recieve 18 chromosomes.

50 points please help !!!!!!!!!!!!!!!!!!!

Answers

Answer:

I think the answer is substitution

Explanation:

Answer:

substitution :)

Explanation:

Transposable elements can induce mutations by all of the following mechanisms EXCEPT: disrupting a gene when inserted into it. facilitating recombination between transposons, producing chromosomal mutations. producing double-stranded breaks during excision, leading to chromosomal mutations. Activating genes with their own promoter, dysregulating them.

Answers

Answer:

Activating genes with their own promoter, dysregulating them.

Explanation:

Transposable Elements (TEs), also known as transposons, mobile elements or jumping genes, are genetic sequences able to move within a host genome by a mechanism called 'transposition'. As a result of their transposition, TEs may cause diverse genetic alterations and disrupt host gene expression at the integration site. Some of the most common deleterious effects induced by TE transposition include, among others, insertional mutagenesis, duplicated sequences, chromosome translocations, etc. Moreover, although it has been shown that some types of TEs contain transcription factor binding sites (as well as cis-regulatory motifs), they don't have promoter sequences that activate host gene expression upon TE transposition.

An object motion changes

Answers

What is this supposed to mean?

Explain how diffusion plays a part in nutrients getting to your cells. !!!!!!!Plz, Answer!!!!!!

Answers

Nutrients attach themselves to diffusion molecules outside of the cell and these molecules will then carry the nutrients across the cell membrane and release them into the cell.

The domain __________________________________ is made up of prokaryotes that usually have a cell wall and the at usually reproduce by cell division.

Answers

a domain made up of prokaryotes that usually have a cell wall and that usually reproduce by cell division

[FW.04H]A single computer microchip uses 10 gallons of water during its manufacture. A group of people suggested that the use of computers should be reduced to help conserve water. Which of these is the best alternative method to conserve water in manufacturing microchips?


Reduce the size of microchips.

Use graywater to manufacture microchips.

Collect rainwater and use it to make microchips.

Research ways to reduce water waste in making microchips.

Answers

Answer:

Option D, Research ways to reduce water waste in making microchips

Explanation:

The best way to conserve any natural resource is to conserve it at the source point and further make it apt for re use.

If the water waste is microchips is reduced then gallons of water will be saved. Also, devising engineering mechanism to reuse water in chip manufacturing industry will further reduce the water waste.

Hence, option D is correct

Use the following table to answer the question:


Element

Mass Number

Atomic Number

Aluminum

27

13

Nitrogen

14

7

Helium

4

2

Fluorine

19

9

please help

Which element has the least neutrons in the nucleus?
Aluminum
Nitrogen
Helium
Fluorine

Answers

Answer:heh

Explanation:

Aluminium , mass no = no of proton + neutron = 27

atomic no = no of proton = 13

no of neutron = 27 - 13 = 14

Nitrogen  , mass no = no of proton + neutron = 14

atomic no = no of proton = 7

no of neutron = 14 - 7 = 7

Helium   , mass no = no of proton + neutron = 4

atomic no = no of proton = 2

no of neutron = 4 - 2= 2

fluorine    , mass no = no of proton + neutron = 19

atomic no = no of proton = 9

no of neutron = 19-9 = 10

Answer:

helium

Explanation:

i took the test and got it right... it also has the smallest number

Describe how soils form layers.

Answers

Answer:

They are produced from rocks (parent material) through the processes of weathering and natural erosion. Water, wind, temperature change, gravity, chemical interaction, living organisms and pressure differences all help break down parent material.

Explanation:

Please answer quick!

Answers

Answer:

im pretty sure its A. have a nice day!

ntroduction: The 19th century was the time of the Industrial Revolution in England. Most of the new industries used coal for energy, and the air was polluted with black soot. In forests near factories, the soot coated trees and killed lichens. As a result, tree trunks became darker. Experiment: Click Play and hunt peppered moths on dark tree trunks for five years. In each year, try to capture as many moths as you can. Why did the changes you observed in the moth populations happen

Answers

Answer:

Light colored peppered moths decreases in number and dark colored peppered moths increases.

Explanation:

The population of light colored peppered moths decreases whereas the dark colored peppered moths increases in number because the light colored peppered moths are visible to the predator birds  whereas the dark colored peppered moths are not visible due to dark coating of the trees so they are saved from the birds and therefore, increase in population of dark colored peppered moths occurs and decrease occur in light colored peppered moths population.

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