pcr lab how are we able to distinguish between the hba beta globin allele and the hbs alleles in our lab exercise?

Answers

Answer 1

We are able to distinguish between the hba (Hemoglobin beta globin) allele and the hbs alleles in our lab because HbA allele produces shorter PCR product than HbS.

Sickle hemoglobin (HbS) is caused by a point mutation in the two subunits of normal hemoglobin (HbA), resulting in nucleated polymerization and pathology.

There are at least two variants (or alleles) of the HBB gene: HbA and HbS. The HbA allele provokes no problems. The HbS variant can result in either sickle cell trait or sickle cell disease. This allele tends to cause red blood cells to "sickle" rather than forming donut shapes.

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Related Questions

the different geometries that a molecule can attain by bond rotations and bends are called conformations.A. TrueB. False

Answers

Referring to: Conformations are the various shapes that a molecule can achieve through bond rotations and bends. True. A.

Other names for it include angular and V-shaped. Examples of molecules with a bent geometry include water (H 2 O), sulphur dichloride (SCl 2), methylene (CH 2), and nitrogen dioxide (NO 2). Because it is not linear and instead has a curved or deformed v-shaped shape, it is called bent. There are two bond pairs and two lone pairings in it. The three-dimensional configuration of the atoms that make up a molecule is known as molecular geometry. It provides details regarding the molecule's overall shape as well as bond lengths, bond angles, torsional angles, and any additional geometrical elements that affect the position.

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4
What is TRUE about race?

A.
It is easy to define in most cultures.

B.
It ignores biological characteristics.

C.
It is socially constructed.

D.
It never changes over time.

Answers

Answer:

Explanation:

i think it A

A because cultures develop more to have and learn about their race

the endocrine system is closely linked with the nervous system. which neurotransmitter can also act as a hormone?

Answers

Sometimes the distinction between hormones and neurotransmitter is not entirely clear. Some neurotransmitters also act as hormones, such as catecholamines (adrenaline, norepinephrine, and dopamine).

Our body employs neurotransmitters, which are chemicals, to convey information from one neuron to another through the synaptic gap that serves as a conduit between them. These signals move back and forth between the central nervous system and the rest of the body with the goal of coordinating our bodies, controlling muscle movement, bodily secretions, and various organ functions. Commonalities among chemical messengers that function as neurotransmitters include their storage in synaptic vesicles, their release in response to the action potential, their binding to membrane receptors on the post-synaptic cell, and their release when calcium ions (Ca2 +) are introduced into the terminal axon. According to the type of receptor they operate on, neurotransmitters' primary job is to either block or promote post-synaptic cell activity. This is done by altering the permeability of the neuronal membrane and its enzymatic activity, which is mediated by other neuromodulators (such as cAMP and cGMP). Sometimes it can be difficult to tell a neurotransmitter from a hormone. Some neurotransmitters, like catecholamines, also function as hormones (adrenaline, norepinephrine, and dopamine).

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FILL IN THE BLANK. blood pressure is highest in the ___________ and lowest in the _____________.

Answers

Answer:

arteries closest to the heart; veins

Explanation:

Blood pressure is highest in the arteries closest to the heart and lowest in the veins.

Compare/contrast DNA and RNA, be sure to include the following:
shape
sugars found
bases found
purpose

Answers

Answer:

C or Bases Found

Explanation:

(DNA contains deoxyribose and RNA contains ribose) and base molecules.

I really hope this helps! Please tell me if it is wrong on your side.

what relatively recent fossil discovery has helped us understand the transition between the lobe-finned fishes and the tetrapods?

Answers

Tiktaalik's recent fossil discovery has helped us understand the transition between the lobe-finned fishes and the tetrapods.

Early lobe-finned fishes are hard fish with plump, lobed, matched blades, which are joined to the body by a solitary bone.

The discovery of well-preserved pelves and a portion of the pelvic fin from Tiktaalik roseae, a 375 million-year-old transitional species between fish and the first animals with legs, indicates that improved hind fins were the actual starting point for the evolution of hind legs.

The non-tetrapod Osteichthyes (bony fish) Tiktaalik was discovered in the Arctic of Canada. It has scales and gills, but it has a triangular, flattened head and unusual, cleaver-shaped fins.

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the principle that two stimuli must differ by a constant proportion for their difference to be perceived is known as

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The principle that two stimuli must differ by a constant proportion for their difference to be perceived is known as Weber's law.

The historically significant psychological law known as Weber's law, also known as Weber-Fechner law, quantifies the perception of change in a given stimulus. According to the law, the slight modification of a stimulus is a constant ratio of the initial stimulus. There is evidence that it does not hold at high levels of stimulation.

The German scientist Ernst Heinrich Weber first proposed the rule in 1834 to describe his studies on weight lifting. Gustav Theodor Fechner, Weber's student, then used the law to measure sensation, which led to the creation of the field of psychophysics. The law told Fechner that there is actually only one world, the spiritual, and that there is a relationship between the spiritual and physical realms. Others saw the law as opening the door to a quantitative, scientific psychology. The joint work of Weber and Fechner has been beneficial, particularly in the study of hearing and vision, and it has affected testing and theoretical advancements such as attitude scaling.

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Which proportionality does Kepler’s third law describe?

Answers

Answer:

According to my search

Explanation:

Kepler's Third Law. Kepler's third law states that the square of the orbital period of a planet is directly proportional to the cube of the semi-major axis of its orbit.

cytochalasin d, a fungal alkaloid, has a similar function, similar to a. profilin b. troponin c. capz d. tropomodulin

Answers

cytochalasin d, a fungal alkaloid, has a similar function, similar to capZ. In muscle cells, CapZ's primary job is to cap the barbed (plus) end of actin filaments.

Various molds secrete a class of compounds known as cytochalasins, which stop cell motility. Cell motility, phagocytosis, microfilament-based trafficking of organelles and vesicles, and the development of lamellipodia and microspikes are all inhibited by the cytochalasins, which bind to the plus end of microfilaments and hinder further polymerization. The sea sponge extract lantrunculin stabilizes and binds to actin monomers, which leads to a net depolymerization of actin filaments. Lantrunculin and cytochalasins both affect actin-based function in a similar manner. Stabilizes microfilaments and prevents depolymerization is phalloidin, an alkaloid isolated from the toadstool Amanita phalloides. These substances also stop cells from moving, showing that actin filament formation and disintegration are both necessary for cell motility.

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if each plant cell is a genetic equivalent of another cell in the same plant, then a horticulturalist could produce a clone of a prize-winning plant by harvesting a cell from ________.

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A horticulturalist could produce a clone of a prize-winning plant by harvesting a cell from the stem of the root of the prized plant.

A cell has 3 elements: the cell membrane, the nucleus, and the cytoplasm. . The nucleus is a structure inside the cell that incorporates the nucleolus and the maximum of the cellular's DNA. it's also in which maximum RNA is made.

Cells are the basic constructing blocks of all residing things. They form shapes for the body, absorb nutrients from meals, and convert those vitamins into electricity, and perform the specialized functions.

Cells provide shape and function for all dwelling matters, from microorganisms to people. Cells residence the organic equipment that makes the proteins, chemical substances, and indicators chargeable for the whole thing that occurs interior our bodies.

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which of these animal taxa has the highest proportion of species threatened with extinction?
a. birds
b. amphibians
c. mammals
d. sharks and rays
e. selected crustaceans
f. reptiles

Answers

c) Amphibians these animal taxa has the highest proportion of species threatened with extinction

Four-limbed, ectothermic animals belonging to the class Amphibia are called amphibians. The class Lissamphibia comprises all currently existing amphibians. They live in many different environments, the majority of which are terrestrial, fossorial, arboreal, or freshwater aquatic settings. Amphibians can dwell on dry ground or in water. Their name derives from the Greek word "amphibios," which is a synonym for "two lives." Amphibians include frogs, salamanders, and toads. Western Africa is home to the Goliath Frog, which is the biggest frog in the world.

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If the herb population in an ecoytem increaed, what mot likely happen to the ize of carnivore

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If the herbivore population in an ecosystem increased, the Carnivore population would also increase.

It is likely that the size of the ecosystem's carnivore population would also grow if the number of herbivores increased. This is due to the fact that carnivores frequently rely on herbivores as their primary food source in an ecosystem. More carnivores would survive to maturity and breed if there was more food for them to eat, which would result in a greater number of newly born carnivores the following season. The number of carnivores would also begin to decrease when their food supplies ran out if this increase caused a decrease in the herbivore population as a result of increased hunting by the increased number of carnivores.

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(complete question)

If the herbivore population in an ecosystem increased, what most likely happen to the size of carnivores?

a forest fire is an example of regulation. a forest fire is an example of regulation. r-selected density-dependent density-independent k-selected

Answers

A forest fire is considered an example of density-independent regulation. Option c is the correct answer.

Regulation that impacts population size without respect to population density is known as density-independent regulation. Most of the time density independent characteristics have nothing to do with the population number and are just physical aspects of the environment. Regardless of population size, density-independent factors always have an impact on the population. Forest fire is a natural disasters. It has nothing to the population size but it affects the population. Because the population's size does not necessarily correspond to how it is affected. Therefore, option c is correct.

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The pelvic splanchnic nerves originate from the neuron cell bodies housed within the lateral gray matter of the ______ spinal cord segments.

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The neuron cell bodies seen in the posterior gray matter of the S2-S4 brain stem segments are the source of the pelvic splanchnic nerves. The nervous system includes them.

The nerve is an organ system that receives and translates sensory data from the environment to coordinate bodily movements.

The systemic nervous system is comprised of the brain and spinal cord.

The 31 parts of the spinal cord include 8 cervicals, 12 disseminate, 5 lumbar, 5 caudal parts, and 1 coccyx.

The sacrum & coccyx, in addition to the cervical, thoracic, and lumbar spine parts, divide the spine's 33 bones, or vertebrates, into five sections.

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Pathogens of the skin often enter via hair follicles and ducts of the sweat glands.
a. True
b. False

Answers

Answer: True

Explanation:

Which of the following bases is not present in DNA Mcq? A
Quinoline
B
Adenine
C
Cytosine
D
Thymine

Answers

the correct answer is A. Quinoline

lacunae can hold what type of cells? osteoblasts and osteoclasts fibroblasts and chondroblasts chondrocytes and osteocytes chondroblasts and osteoblasts

Answers

Lacunae can hold chondrocytes and osteocytes types of cells. Osteocyte bone cells are found in the lacuna, which is the area between these rings.

The bone cells (osteocytes), which are situated between the rings of matrix, are found in regions known as lacunae.

Lacunae are home to osteoblasts. The lacunae known as Howship's lacunae are occasionally observed to contain enormous multinucleated osteoclasts, which break down bone. These are easily located in the growth plate's ossification zone. In this slide, the spongy bone and marrow cavity are encircled by the solid bone.

A lacuna in bone or cartilage serves primarily as a home for the cells it contains, preserving their viability and functionality. Osteocytes are enclosed by lacunae in bones, and chondrocytes are enclosed by lacunae in cartilage.

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How do you explain the basis for classification of organisms using evidence from morphology, anatomy, physiology, behavior, molecular biology, genetics, or evolution?

Answers

Organisms can be classified based on a wide range of evidence.

What is molecular biology?

Molecular biology is a branch of biology that deals with the structure, function, and interactions of molecules in living systems. It combines chemistry, biochemistry, genetics, and biophysics to study the structure and function of cellular components such as proteins, DNA, and RNA. It also looks at how cells in different organisms interact to form complex systems, as well as how they interact with their environment. It is an interdisciplinary field of study that helps to explain how living organisms work at the molecular level.

Morphology involves the study of shape and form, while anatomy studies the internal structure of organisms. Physiology looks at the processes and functions of the organism, and behavior examines how the organism interacts with its environment. Molecular biology studies the structure and function of molecules within the organism, genetics looks at the inheritance of traits, and evolution examines the changes that occur over time. Collectively, these aspects provide important evidence for the classification of organisms.

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when using dna center, what is it called when you are leveraging the rest api or the gui interface of dna center?

Answers

When using the DNA center's rest API or GUI interface, northbound APIs are used.

Describe northbound REST API.

In contrast, a southbound interface allows a higher-level component to send commands to lower-level network components. A northbound interface is an application programming interface (API) or protocol that enables communication between lower-level and higher-level or more central network components.

The communication channel between the applications and the SDN controller is provided by northbound APIs. Applications can inform the network of their resource requirements (data, storage, bandwidth, etc.), and the network can communicate what it has or deliver the resources requested. Numerous applications are supported by these APIs.

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what portion of the flower will be purple

Answers

Answer: the light lilac to deep violet.

Explanation:

how could having too many growth factor receptors on the surface of the cell make a more aggressive cancer

Answers

The cell makes a more aggressive cancer High levels of a growth factor increase the risk for several cancers.

Overactivation of these signaling pathways resulting from amplification, overexpression, or mutation of growth factor receptors occurs in various cancers. These alterations in growth factor signaling pathways contribute to cancer development, progression, angiogenesis, and metastasis.

Cancer is a disease that occurs when cells divide uncontrollably and spread to surrounding tissue. Cancer is caused by changes in DNA. Most of the DNA changes that cause cancer occur in sections of DNA called genes. These changes are also called genetic alterations. Tumor growth inhibitors are also called cancer growth inhibitors. They are a type of targeted cancer drug.

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FILL IN THE BLANK. animals that can detect ultraviolet (uv) light differ from humans by having ________.

Answers

Thicker retinas that can capture UV light more efficiently and UV-specific opsins that extend their visible light spectrum.

In general, light having wavelengths between 380 and 700 nanometers may be seen by humans (nm). Within that range are all the hues of the rainbow, from red all the way to violet. UV light, however, has wavelengths that are shorter than 380 nm. They are hence invisible to the human sight.

Is everyone blind to UV light? No, actually. Aphakia is a condition that causes people to be able to see UV light rays. Aphakia is the absence of an eye lens, which is frequently the result of surgery or heredity. They can see beyond the visible spectrum of light since they don't have this lens, but it also results in fuzzy vision and farsightedness.

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Consider a population in which the frequency of allele AA is p=0.7p=0.7 and the frequency of allele aa is q=0.3q=0.3, and where the alleles are codominant. What will be the allele frequencies after one generation if the following occurs?wAA=1wAA=1 , wAa=0.9wAa=0.9 , and waa=0.8waa=0.8 . Enter your answers in the following order: qg+lqg+l , pg+lpg+l .

Answers

The answer to this question is q[tex]_{g+1}[/tex] = 0.278 and p[tex]_{g+1}[/tex] = 0.722

The population given is in Hardy Weinberg equilibrium (HWE). The principle applies if a population follows conditions such as no mutation, no selection, large population size, no migration, and random mating. If all conditions are followed by a population, its genotypic and phenotypic frequencies remain constant from generation to generation.

The HWE equation is as follows:

p² + 2pq + q² =1

Where p² = homozygous dominant frequency, pq = heterozygous frequency, q² = homozygous recessive frequency.

The allele given is codominant. The allele frequency after one generation can be calculated using the formula:

q = [tex]\frac{(W_{Aa})(p)(q) + (W{aa)}(q^2)}{W_{AA}(p^2) + (W_{Aa})(2pq)+(W_{aa})(q^2)}[/tex]

here

p = 0.7

q = 0.3

W[tex]_{AA}[/tex] = 1

W[tex]_{Aa}[/tex] = 0.9

W[tex]_{aa}[/tex] = 0.8.

So:

q[tex]_{g+1}[/tex] =  [tex]\frac{(W_{Aa})(p)(q) + (W{aa)}(q^2)}{W_{AA}(p^2) + (W_{Aa})(2pq)+(W_{aa})(q^2)}[/tex]

q[tex]_{g+1}[/tex] = [tex]\frac{(0.9)(0.7)(0.3) + (0.8)(0.3)}{(1)(0.7) + (0.9)(2(0.7)(0.3)+(0.8)(0.3)}[/tex]

q[tex]_{g+1}[/tex] = 0.261/0.94

q[tex]_{g+1}[/tex] = 0.278

p[tex]_{g+1}[/tex] = = 1 - 0.278

p[tex]_{g+1}[/tex] = 0.722

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receptors that bind the neurotransmitter at the postsynaptic cell membrane are voltage-gated. t or f

Answers

Receptors that bind the neurotransmitter at the postsynaptic cell membrane are voltage-gated.

This statement is false.

Neurotransmitters are chemical messengers that your frame can not function with out. Their process is to hold chemical signals (“messages”) from one neuron (nerve cellular) to the following goal mobile. the following goal cellular may be another nerve mobile, a muscle cellular or a gland.

Despite the fact that there are numerous unique minor and principal neurotransmitters, we can cognizance on those important six: acetylcholine, dopamine, norepinephrine, serotonin, GABA, and glutamate.

Glutamate (Glu) is the maximum effective excitatory neurotransmitter of the relevant nervous device which guarantees homeostasis with the effects of GABA.

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suppose that a single dna base change of an ""a"" to a ""t"" occurs and is copied during replication. is this change necessarily a mutation?
a. Traffic Congestion
b. Device Manipulation
c. Attack Surface
d. Environmental Monitoring

Answers

The above options are incorrect as it is a change in the DNA sequence, during replication. is this change necessarily a mutation

A gene variation is an replication to a gene's DNA sequence that takes place throughout time. Gene variation is regarded to be a more accurate word for this sort of genetic change than gene mutation since changes in DNA do not necessarily result in illness. A mutation is a change to an organism's DNA sequence. Mutations can be the consequence of viral infection, exposure to mutagens, or mistakes in DNA replication during cell division.

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a plant homozygous for the unlinked dominant alleles a, b and g is crossed with another plant that is homozygous for the recessive alleles a, b and g. how many different genotypes in the f2 population will give rise to the abg phenotype?

Answers

The abg phenotype is produced by 4 distinct snps in the f2 population.

What are the three genotype types?

There are three distinct genotype types: homozygous dominant (PP), homozygous recessive (PP), and homozygous (Pp). The traits are the same in homozygous dominant and heterozygous genotypes.

What are the four genotype types?

Humans have four different hemoglobin genotypes, or hemoglobin pairings and formations: AA, AS, SS, and AC (uncommon). The aberrant genotypes or sickle cells are SS and AC. Each of us have a particular pair of these hemoglobin, which we acquired from both parents, in our blood.

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During which phases of the cell cycle is the cell duplicating its genetic material and organelles?

Answers

The cell duplicates its genetic material during the S phase, and organelles during the G2 phase of the cell cycle.

During the G2 phase of the cell cycle, the majority of organelles duplicate. After the S phase, during which DNA or genetic material is duplicated, comes this phase. Additionally, this stage happens before mitosis, where the cell parts into two identical daughter cells.

In addition to the replication of organelles in preparation for mitosis, cell growth occurs during the G2 phase. The majority of the G2 phase, however, is spent preparing for mitosis. The cytoskeleton of the cell is taken apart to be used for the division of genetic material and organelles, and energy is also stored for cell division.

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identify the space that can be surgically accessed through the posterior fornix of the vagina.

Answers

Answer:The rectouterine pouch

Explanation:The rectouterine pouch is a low point in the peritoneal cavity and may be a location of infection, pooled blood, and serous fluid. The rectouterine pouch can be drained surgically through the posterior fornix of the vagina.

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gerusa is a company of former soviet scientists who use a special chemical to kill cancer cells. while the scientists have the knowledge, they are short on equipment, money, and marketing know-how. it would seem that gerusa should

Answers

While the scientists have the knowledge, they are short on equipment, money, and marketing know-how. it would seem that gerusa should Find a partner with whom they can work together.

They should look for an investor or partner with whom they can work together to improve and develop their product.

Gerusa has developed a distinct chemical that can kill cancer cells, giving it a competitive edge in the market. If it chooses to work with a partner, it will have to share all of its resources and the technology for its chemical. This will make it less able to control the technology because the partner will also have information about the method and the solution. As a result, Gerusa has complete command of the technology when it decides not to partner with anyone else.

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Pooja has green eyes while her parents and brother have black eyes. Pooja’s husband Ravi has black eyes whil his mother has green eyes and his father has black eyes.

On the basis of the above given information, is the green eye colour a dominant or a recessive trait? Justify your answer.

What is the possible genetic makeup of pooja’s brother’s eye colour?

50% of the offspring of Pooja’s brother are green eyed.with the help of cross show how this is possible.

Answers

The green eye color is a recessive trait because Pooja's parents and brother have black eyes, and recessive traits are expressed only in homozygous (parents are heterozygous).  The possible genetic makeup of Pooja’s brother’s eye color is homozygous dominant AA or heterozygous Aa.

What is a homozygous dominant individual?

A homozygous dominant individual is one that has a domain allele by duplicated instead of another allele for the same locus. In a heterozygous individual, the dominant allele masks the expression of the recessive allele that causes the trait such as occurs in green eyes that are expressed in recessive homozygous individuals.

In the cross here we can be denoted the domain black eye alle as 'A' and the recessive green eye alle as 'a', thereby the cross between heterozygous parents is:

           A      a

A       AA     Aa

a       Aa      aa

Therefore, with this data, we can see that the green eye color is recessive.

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the demand for a product for january is 520 units and the seasonal index for this time period is 0.8, find the deseasonalized sales for this period. Find the value of x in the figurePlease show all your work. Calculate the area, in square units, bounded by f(x) = 3x^3-3x^2-x+8 and g(x) = 2x^3 - 10x^2 + 29x + 8 over the interval [1, 5]. What is the main reason that the gravitational attraction between Earth and the Moon changes each day?(1) Earths axis is tilted at 23.5.(2) Earths rotational speed varies with the seasons.(3) The Moon has an elliptical orbit.(4) The Moon has a spherical shape. as part of their investment strategy, the carringtons have decided to put $100,000 into stock market investments and also into purchasing precious metals. the performance of the investments depends on the state of the economy in the next year. in an expanding economy, it is expected that their stock market investment will outperform their investment in precious metals, whereas an economic recession will have precisely the opposite effect. suppose the following payoff matrix gives the expected percentage increase or decrease in the value of each investment for each state of the economy. how did spain respond to french interest in extending their lands past the mississippi river valley in the early 1700s? the return on investment (roi) is measured by dividing the amount of operating income by the amount of the return. this statement is If i have 2 apples and i get 69 times that, than i give three-ninths of it to a friend, how much would i have left? when the term reputation management is used, it is applicable solely during a crisis. produces a temporary change in public opinion. includes publicity and press agentry. is a superset of public relations. is a short-term tactical process. Need help solving this a type of director that provides the controlling vision for their films from idea, through script writing, to completion, closely supervising most every step in the process is called... if you desire to have $30,000 for a down payment for a house in eight years, what amount would you need to deposit today? assume that your money will earn 6 percent. What is the benefit of sketching or providing a screenshot of the DNA fingerprints for qualitative data? What is the benefit of gathering quantitative data on the base-pair lengths? Describe one struggle a forensic scientist might have when looking at DNA evidence? A carboxylase reaction by rubisco consumes ______ ATP, and an oxygenase reaction consumes ______ ATP. Where is Santas first stop? Do Asians have a Christmas? find the definite integral of the function f shown in the graph below, over the limits from 0 to 3. Which of the following is not one of the twelve morphological classifications recognized by structural grammarians?- NOT: function word, transform- ARE: noun, verb, adjective, adverb, auxiliary, preposition, determiner, conjunction, pronoun, interrogative, intensive, unnamed class At constant T and P, 2.0 L of nitrogen gas are combined with 6.0 L of hydrogen gas to form ammonia. Which of the following statements correctly describe the outcome of the reaction, assuming it goes to completion? Select all that apply.When the reaction is completed, only NH3 is present.The product occupies a volume of 4.0 L under these conditions.N2 combines with H2 in a 1:3 ratio. The plant and production benchmarking cost data in each issue of the Footwear Industry Report A. are less useful than the operating benchmarks in determining whether a company is managing certain aspects of its plants in a cost efficient manner. B. provide solid evidence of whether a company is managing certain aspects of its plants cost-efficiently Care more useful to company managers if the company has adopted a strategy to produce branded footwear with a 3-star S/Q rating or higher C. are most useful to the managers of companies that have adopted a strategy to be the low-cost provider of private-label footwear D. provide solid evidence of whether a company's total manufacturing costs of branded footwear (and private-label footwear. If any pairs are being produced) are low enough to enable the company to be attractively profitable