Option C, Contribute to herd immunity the following ways do vaccines promote public health.
Vaccines, When a sufficient number of people are immunized against a disease and have produced protective health antibodies against upcoming infection, herd immunity can also be attained. Vaccines build immunity without resulting in disease or other negative side effects on health, in contrast to the natural health infection technique. The incidence and spread of the illness are reduced as the population becomes more immune. Indirect protection from illness is provided by immunizations for others.
The complete Question is:
In which of the following ways do vaccines promote public health?
A) Reduce the number of fatalities due to infection.
B) Prevent cancer development following infection with an oncogenic virus
C) Contribute to herd immunity
D) Prevent Type I diabetes (T1D)
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use the labels to correctly identify components involved in the maintenance of the resting membrane potential.
The elements involved in maintaining the permeability of the cell membrane include the K+ leak channel, Na+ leak channel, Na+-K+ pump, and ATP ADP-K+.
What does a human membrane do?Body membranes are incredibly thin tissue sheets that line body cavities, cover organs inside of hollow organs, and cover the body's surface. The epithelium and connective tissue walls are the two basic types of bodily membranes. Mucous membranes, peritoneal glands, synovial glands, and meninges are subcategories.
What in biology is a membrane?A bilayer of lipid molecules makes up the double sheet that makes up biological membranes. Generally speaking, this structure is known as the lipid bilayers. Membrane proteins and glucose are important components of biological membranes in addition to the different lipids that are found there.
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g you have a different mutant mouse who has low blood glucose in response to stress and you do not know where the mutation is. to help find out, you remove their pituitary gland and transplant a normal pituitary gland into this mouse. afterward this mouse still has low blood glucose in response to stress. given this data, this mouse might have a mutation in . . .group of answer choicescrhthe crh receptoractheither the crh receptor or acth
According to the given information,mouse might have mutation in a)CRH. So,correct option is a.
Hereditarily designed mice have instigated changes, including the transgenes, designated transformations (knockouts or knockins), and retroviral, proviral or the artificially actuated mutations. Transgenic mice convey a portion of unfamiliar DNA integrated into their genome through the non-homologous recombination (e.g., pronuclear microinjection), disease with the retroviral vector or the homologous inclusion.
From the diagram, it can see that mouse have mutation in CRH, where the value of hypothalamus is very high. Mutations is favored when quantity of hypothalamus is very high. Corticotropin- releasing hormone (CRH) is considered as the significant controller of the hypothalamic-pituitary-adrenal (HPA) hub, includes the wide range of activities inside the focal sensory system and the fringe. The turn of the events and utilization of Crh knockout mice (Crh-/ - ) has been the significant instrument for tending to the physiologic and pathologic jobs of CRH.
Hence, option a is correct.
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(Complete question) is:
You take a different mutant mouse who also has a low blood glucose in response to stress. To help find out, you remove their pituitary gland and transplant a normal pituitary gland into this mouse. This time, the mouse's blood glucose goes back to normal. Given this data, this mouse might have a mutation in A) CRH B) the CRH receptor C) ACTH D) Either the CRH receptor or ACTH
The Golgi tendon organ (GTO) is a nerve found in muscles. One of the GTO’s tasks is to create a reflex that limits the tension placed upon a muscle.
What is the effect of plyometric training on this GTO reflex?
Plyometric training increases the effect of the GTO reflex.Plyometric training increases the effect of the GTO reflex. , ,
Plyometric training functions within the control of the GTO reflex.Plyometric training functions within the control of the GTO reflex. , ,
Plyometric training is not involved with the GTO reflex.Plyometric training is not involved with the GTO reflex. , ,
Plyometric training reduces the effect of the GTO reflex.Plyometric training reduces the effect of the GTO reflex. , ,
The main effect of plyometric training on this GTO reflex is that it reduces the effect of the GTO (Golgi Tendon Organ) reflex. Thus, the correct option is D.
What is the effect of plyometric training on GTO reflex?The Golgi tendon organ (GTO) is found in the tendon which is present near the junction of tendon and muscle fibers in the body. It responds to a tendon stretch, or a muscle contraction, by sending the action potentials so that muscle tension increases in these junctions.
Plyometric exercises are the activities which have been shown to be an effective method of improving a number of different physical qualities such as strength and jump height, running economy, agility, sprint speed and endurance of a person. The exercises or activities which are involved in Plyometric exercise are characterized by explosive muscle extension and contraction mechanisms.
Plyometric training is found to effect the GTO reflex as it reduces the effect of the GTO reflex.
Therefore, the correct option is D.
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3. What is the probability of a type BO mother and a type AO father having a type O child?
Answer:
B: 75% ////// O: 25%
Explanation:
So each child has a 75% of being B and a 25% chance of being O. Note that genetically, not all of the B blood type kids are the same. The BB child will only pass down the B version of the gene. The BO child can pass on their O gene version.
which of the following terms describes the functional classification of the illustrated joint with regard to its range of movement?
The term diarthrosis describes the functional classification of the illustrated joint with regard to its range of movement.
Which of the following best exemplifies the functional rankings of joints?According to their functional classification, the three types of joints are synarthroses, amphiarthroses, and diarthrosis. Synarthroses prevent any motion between the articulating bones.
Give instances of the many joint types you have examined and explain what you mean by the term joint name?
A joint, also known as an articular surface, is a place in the human skeletal system where two or more bones link. Cartilage is a type of tissue that keeps two neighboring bones coming in touch with each other. Synovial joints, fibrous joints, and cartilaginous joints are the three different types of joints.
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recombinant dna contains a segment of foreign dna is defined as a mix of human and animal dna is created from embryonic stem cells is a technique for repairing damaged dna
A DNA molecule made in vitro that contains sequences from two or more sources or organisms is known as recombinant DNA.
recombinant DNA is the process of inserting DNA molecules from two distinct species into a host organism in order to generate novel genetic combinations that are beneficial to agriculture, industry, science, and medicine. The primary objective of laboratory geneticists is to isolate, characterize, and manipulate genes because the gene is the primary focus of all genetics. In spite of the fact that it is moderately simple to detach an example of DNA from an assortment of cells, finding a particular quality inside this DNA test can measure up to tracking down a tough-to-find little item. Take into consideration the fact that the DNA in each human cell is approximately 2 meters (6 feet) long. As a result, a large amount of DNA will be present in a small tissue sample.
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_____ are a collection of infected hair follicles that often occur on the back of the neck, the upper back, and lateral thighs that form a mass that evolves into an erythematous, painful, edematous mass, which drains through many openings.
a. Erysipelas
b. Furuncles
c. Carbuncles
d. Boils
c. carbuncles
(c) Carbuncles are a collection of infected hair follicles that often occur on the back of the neck, the upper back, and lateral thighs that form a mass that evolves into an erythematous, painful, edematous mass, which drains through many openings.
Hair follicles are a small tube like opening or pore that inhabit the roots of the hair strand. The top two layers of the skin together form the hair follicle. Around 20 types of cells together form the hair follicle.
Erythematous refers to the redness of the skin or the mucous membrane due to the accumulation of blood in the dilated capillaries of that region. The condition can arise either due to some injury or due to infection.
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If you added one more proton to an atom of silver, then what element would it become? Palladium (Pd) Cadmium (Cd) Gold (Au)
Why is the liver important in how effective a drug is? Hint - one of the liver structures listed in the picture is a clue!
The bulk of drugs and poisons that are brought into the body are cleared by the liver and then ingested. While certain parent medications might cause hepatotoxicity, most drug-induced liver fires are caused by drugs' metabolites (DILI).
What role does the liver play?All of the blood that leaves the intestinal and stomach is received by the liver .The liver breaks down, balances, and delivers nutrients while also processing this blood. In addition, it metabolizes medicines to make them toxic or quicker for the body to absorb.
Can I survive if my liver fails?Without a functioning liver, you cannot survive. You may require a liver transplant if your liver no longer functions correctly. If your liver disease has reached its latter symptoms, a kidney transplant may be advised (chronic liver failure). This liver condition is severe and potentially fatal.
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Which of the following explains why the human population has so many phenotypic differences?
A) The majority of our genome consists of noncoding DNA.
B) The majority of our genome consists of DNA that codes for proteins.
C) Recombination allows for new genetic combinations.
D) The genes that code for what we look like are more susceptible to mutation than other genes.
E) None of the answer options are correct.
The right answer, based on the above statement, is that recombination results in novel genetic combinations.
What does a human genome look like?The total revenue of DNA instructions existing in a cell makes up the genome. The genetic information is made up of 23 sets of chromosomes that are found in the dna and one tiny chromosome that is found in the mitochondria. All that a person needs to develop and operate is encoded in their DNA.
How many genomes are there in humans?The genome is divided into 23 pairs of sex chromosomes (XX there in female and (XY) as in male), as well as 22 paired chromosomes, known as autosomes.
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the sense of taste is also known as the sense of taste is also known as maculation. sublation. olfaction. gustation. proprioception.
Gustation is another name for the taste sense.
What in biology is gustation?Gustation is the term used to describe the recognition of taste with sensory neurons in the oral cavity, primarily on the tongue. The nose is covered with thousands of tiny bumps known as papillae, and each one of these contains millions of taste buds.
What is the gustation process?Gustation is the gustatory system's perception of the flavor or flavors of food that has been consumed. When molecules in food or any other substances interact with taste receptor cells found in the taste buds, taste is produced. In the oral mucosa, particularly on the tongue, are the taste buds.
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If a skeletal muscle cell has depleted its stores of ATP how will the altered transport properties of the NKA transporter affect cytosolic calcium concentrations (increase, decrease, no change) relative to normal? Why?
The sodium potassium ATPase (NKA), which pumps 3 sodium out and 2 potassium in, becomes inactive during ATP depletion. The effect is an increase in intracellular calcium levels.
Inhibiting the reciprocal interchange of sodium (into the cell) and calcium (out of the cell), the intracellular sodium levels rise as a result.Ions are moved into and out of cells by the sodium-potassium pump. Both during and after stimulation, it helps keep a cell's resting potential constant. Maintaining a low sodium and high potassium content within the cell controls the potential of the cell membrane.Amino acids, glucose, and other vital nutrients are transported via a large number of secondary active transporters, which are membrane-bound transport proteins that are triggered by Na export.The sodium-potassium pump keeps the osmolarity of cells constant, which controls cell volume. Cell volume is regulated by osmosis. This process keeps the concentration of different nutrients and chemicals under control.To know more about sodium check the below link:
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The function of Peyers patches is ?
Answer:
Explanation:
Peyer's patches play an important role in immune surveillance of materials within your digestive system. Immune surveillance refers to the process by which your immune system recognizes and destroys potential pathogens.
t cells are differentiated into two groups based on their glycoproteins: cd4 or cd8. which of the following is true of cd4 t cells?
According to the supplied remark, t cells were differentiated into either the cd4 or cd8 group depending on their glycoproteins. They develop into plasma cells.
What use does a glycoprotein serve?The immune system is one of several physiological processes that are affected by glycoproteins, which are molecules made up of protein and carbohydrate chains. Most viruses contain glycoproteins that aid in their ability to enter host cells and which can also be useful therapeutic or prophylactic targets.
What exactly are glycoproteins and where are they made?A protein and a covalently linked carbohydrate. Glycoproteins, which are created during the glycosylation process in the Golgi apparatus, are crucial parts of plasma membranes, where proteins extend all through the lipid bilayer.
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suppose an organism with genotype aabbee undergoes a meiotic non-disjunction in meiosis i of the chromosomes that carry the a gene. all three genes assort independently on different chromosomes. if the gametes produced by this organism fused with gametes of an organism with genotype aabbee, indicate the percentage of zygotes with each of the following genotypes that are formed:
Suppose an organism with genotype aabbee undergoes a meiotic non-disjunction in meiosis i of the chromosomes that carry the a gene. 68% of zygotes with each of the following genotypes that are formed.
A chromosome is a lengthy DNA molecule that contains all or a portion of an organism's genetic code. The very long, thin DNA fibers in most chromosomes are covered with packing proteins; in eukaryotic cells, the histones are the most significant of these proteins. In order to preserve the integrity of the DNA molecule, these proteins condense and adhere to it with the help of chaperone proteins. These chromosomes exhibit a complicated three-dimensional structure that is crucial for controlling transcription. Under a light microscope, chromosomes are typically only discernible during the metaphase of cell division (where all chromosomes are aligned in the center of the cell in their condensed form). Sister chromatids are the new name for the linked copies. A metaphase chromosome is an X-shaped structure that forms during metaphase and is highly compressed, making it the easiest to recognize and study. Chromosomes are most compacted in animal cells during the chromosome segregation process known as anaphase. Genetic diversity is greatly influenced by chromosomal recombination during meiosis and subsequent sexual reproduction.
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which of the following is true regarding the electron transport chain? (select all that is/are true) group of answer choices it occurs in the cytoplasm of a cell. it occurs in the mitochondria of a cell protons are actively pumped from the inner membrane space to the matrix via the ets complexes. molecular oxygen (o2) is required for it to operate nadh donates a hydride to complex i, which donates electrons to complex iii then to complex iv with o2 being the final electron acceptor.
It occurs in the mitochondria of a cell protons are actively pumped from the inner membrane space to the matrix via the ets complexes, is the correct statement about the electron transport chain.
Mitochondria ar membrane-bound cell organelles (mitochondrion, singular) that generate most of the energy required to power the cell's organic chemistry reactions. energy made by the mitochondria is keep in a very little molecule known as ATP (ATP).
The mitochondrial matrix is that the website of the tricarboxylic acid (TCA) cycle, a series of catalyst reactions initiated by the conversion of pyruvate and fatty acids to acetyl group coenzyme A (acetyl-CoA). Pyruvate and fatty acids ar transported into mitochondria from the protoplasm by membrane-bound permeases.
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what are the biochemical cycles
Answer:water, carbon, nitrogen, and phosphorus
Svetlana asks Dr. Miller for information related to Alzheimer’s disease. Dr. Miller decides to send Mary Ellen, an RMA, into the room to review a patient education brochure that discusses the progression of the disease. What information should this brochure include?
This brochure should indicate the stages of Alzheimer’s disease progression.
What is the Alzheimer’s disease?The Alzheimer’s disease is described as a progressive disease that destroys memory and other important mental functions.
Alzheimer's is a degenerative disease that affects the elderly. The cause of the Alzheimer’s disease is still not yet known, but it is believed that it is influenced by genetic factors.
This Alzheimer’s disease causes degeneration of the frontal and temporal lobes, impairing the individual's cognitive functioning.
The disease has four stages of development which include:
pre-dementia, early stage, intermediate stage, and terminal stage.There is still no Alzheimer's disease but it can be treated with anticholinesterase drugs that aims to delay symptoms and degeneration.
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operon operator repressor regulatory gene corepressor trp operon tryptophan lac operon allolactose inducible operon vs. repressible operon activator inducer cyclic amp positive vs. negative gene regulation differential gene expression histone acetylation dna methylation epigenetic inheritance control elements micrornas small interfering rnas nucleic acid hybridization in situ hybridization
Operons are a type of gene regulatory structure found in prokaryotes. They consist of an operator and a series of structural genes that are transcribed together as a single mRNA. The operator is a DNA sequence that binds repressor proteins, which can then bind to the operator and prevent transcription of the structural genes.
There are two types of operons: the trp operon, which controls the synthesis of tryptophan, and the lac operon, which controls the metabolism of lactose.
The repressor proteins that bind to the operator sequences of operons can be either repressible or inducible. Repressible operons are activated by a corepressor molecule, which binds to the repressor and causes it to unbind from the operator. Inducible operons, on the other hand, are regulated by an activator molecule, which binds to the repressor and causes it to bind to the operator, thus activating transcription.
In addition to operons, gene expression can also be regulated by other mechanisms. Cyclic AMP (cAMP) is a molecule that can bind to DNA and activate transcription. This process is known as positive gene regulation. Negative gene regulation, on the other hand, is the process by which a repressor protein binds to a DNA sequence and prevents transcription.
Differential gene expression is the process by which the same gene is expressed at different levels in different cells or tissues. This can be regulated by epigenetic modifications, such as histone acetylation and DNA methylation. These modifications are heritable, and can be passed on from one generation to the next, leading to a form of epigenetic inheritance.
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Explain in detail?
in the short-term, gains in ___ are a good indicator of nutritional status. the best indicator of long-term nutritional status in a child is gains in .
A person's intake of energy and/or nutrients can be deficient, excessive, or unbalanced in the case of malnutrition. The phrase "malnutrition" refers to
Anthropometric tests to evaluate growth and development, particularly in young children, are the most commonly used markers of nutritional status in a community. Doctors use the medical terminology "overweight" or "obesity" to determine if someone has a. Indicators of nutritional status for the CDC Growth Charts include obesity, overweight, underweight, and short stature. The percentage of low height-for-age indicates the cumulative impact of undernutrition and infections since birth, and even before birth. On a growth chart, percentiles are used to rank people or groups and show where they fall.
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You sample the solid waste of three infants from three different families each week for 24 weeks. You find that the population of bacteria in the gut is dominated by one species for the first 12 weeks, and then shifts to another species. This shift in the biological composition of a community over time is called:
A. competition.
B. mutualism.
C. succession.
D. evolution.
E. ecology.
This shift in the biological composition of a community over time is called succession.
The process by which a region's species and habitat mix changes over time is referred to as ecological succession. These communities gradually replace one another until a "climax community" is reached, such as a mature forest, or a disturbance occurs, such as a fire. Ecological succession is a fundamental concept in ecology. The first species to appear in a new environment are mosses and lichens.
They prepare the ground for larger species to grow, such as grasses, shrubs, and, eventually, trees. Ecological succession is essential for the growth and development of an ecosystem. It begins colonization of new areas as well as recolonization of areas destroyed by biotic and climatic factors. As a result, organisms can learn to adapt to changes and survive in a constantly changing environment.
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use the list of choices below for the following question: i. helicase ii. dna polymerase iii iii. ligase iv. dna polymerase i v. primase which of the enzymes covalently connects segments of dna?
Enzymes which covalently connects segments of DNA is v)primase. So, correct option is v.
Primase is a catalyst that blends short RNA groupings called groundworks. These preliminaries act as a beginning stage for DNA union. Since primase produces RNA particles, the protein is a kind of RNA polymerase. Primase capabilities by integrating short RNA groupings that are reciprocal to a solitary abandoned piece of DNA, which fills in as its format.
Primase is important that groundworks are blended by primase before DNA replication can happen. This is on the grounds that the chemicals that incorporate DNA, which are called DNA polymerases, can join new DNA nucleotides to a current strand of nucleotides. Hence, primase serves to prime and establish a groundwork for DNA blend.
Hence, option v is correct.
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which of the following represents inversion chromosomal mutation in this sequence of dna 5' c t a g t t g c a a t 3' 5' c t a t t g g c a a t 3' 5' c t a g g c a a t 3' 5' c t a g t t g t g c a a t 3' 5' c t a g t t g c a a t 3'
the cell bodies of the neurons projecting to the primary visual cortex are located in the lateral geniculate nucleus.
The Lateral Geniculate Nucleus (LGN) is located in the thalamus and is the primary pathway for conveying visual information from the retina to the primary visual cortex. It is composed of six layers of neurons, with neurons in each layer projecting to different layers of the primary visual cortex.
Step 1: The neurons projecting to the primary visual cortex are part of the retinotectal pathway, which is a neural pathway that transmits visual information from the retina to the brain.
Step 2: The retinotectal pathway begins at the retina, which is the light-sensitive layer of tissue at the back of the eye.
Step 3: The neurons in the retina send signals to the lateral geniculate nucleus (LGN), which is a brain structure located in the thalamus.
Step 4: The LGN is the relay station for the retinotectal pathway and is composed of several layers of neurons.
Step 5: The neurons in the LGN receive visual information from the retina and send this information to the primary visual cortex, which is located in the occipital lobe of the brain.
Step 6: The cell bodies of the neurons projecting to the primary visual cortex are located in the LGN.
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marco and natalia, two curious students, are interested in what happens in a plant seed very early in germination - before anything turns green. they put seeds dehydrated in a dark sealed container and measure (a) the amount of lipids and carbohydrates in the seed and (b) the amount of oxygen and co2 in the headspace (see the experimental setup below). they add water to initiate germination. very early after adding water they remeasure (a) and (b) from above. their data is displayed in the charts below.
Through respiration, the seed uses CO2 to oxidize some lipids to CO2 and transforms the remaining lipids to produce the majority of the increase in carbohydrates.
The ideal response is b.
How does a plant germinate?Germination is the process by which a seed transforms into a seedling. To germinate, seeds require the correct amount of moisture, oxygen, and temperature. When seeds are in a dormant state, they wait to germinate when the environment is favorable for their survival and growth.
What three phases make up germination?The germination process involves three main steps. These include increased metabolic activity, hydration, and cell swelling. The seed imbibes (absorbs) water to start the germination process.
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For the difference between the domestic tomato, Solanum esculentum, and its wild South American relative, Solanum chmielewskii, the environmental variance accounts for 13 percent of the total phenotypic variance of fruit weight, for 9 percent of the total variance of soluble-solid content, and for 11 percent of the total variance in acidity. What are the broad-sense heritabilities of these traits?
A sexually compatible wild tomato species known as Lycopersicon parviflorum has mostly gone unused in tomato breeding. In an interspecific cross between the wild species L.
Parviflorum and the elite processing tomato Lycopersicon esculentum E6203, the Advanced Backcross QTL (AB-QTL) technique was employed to search this genome for QTLs controlling traits of agronomic value (LA2133). 133 genetic markers were used to genotype a total of 170 BC2 plants (131 RFLPs; one PCR-based marker, I-2, and one morphological marker, u, uniform ripening).
In duplicated field trials conducted in California, Spain, and Israel, about 170 BC3 families were cultivated. They were then graded on 30 horticultural features. A total of 199 significant putative QTLs, ranging from 1 to 19 QTLs discovered for each characteristic, were found for all the traits. Despite the overall inferior phenotype of the wild species, at least one QTL was found for which the L. parviflorum allele was associated with an agronomically favorable effect for 19 (70%) traits (excluding traits for which effects of either direction are not necessarily favourable or unfavourable).
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while the____will be most active during the encoding of a face stimulus, the____will be active during the delay period.
The answer to this question is fusiform gyrus and prefrontal cortex respectively.
What is fusiform gyrus?The temporal and occipital lobes' basal surfaces are connected by a structure called the fusiform gyrus, also called the occipitotemporal gyrus.
It is the biggest part of the human ventral temporal cortex, a functionally distinct area important for categorization of visual stimuli.
The fusiform gyrus has been linked to high-level visual processing activities, namely the processing of data related to faces, bodies, and stimuli with high spatial frequency, even though its exact role is still unknown.
The relationship between the fusiform gyrus' physical makeup and its physiological role is still up for debate.
Clinical applications including surgical planning for glioma removal and awake craniotomies may benefit from clarification of the fiber-bundle anatomy and functional linkages present in the fusiform gyrus.
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select the characteristics of slow-twitch muscle fibers. check all that apply. check all that apply few capillaries are present.few capillaries are present. these fibers are also known as white fibers.these fibers are also known as white fibers. myoglobin is present in high concentration.myoglobin is present in high concentration. mitochondria are numerous in these fibers.
The characteristics of slow-twitch muscle fibers are : myoglobin is present in high concentration and mitochondria are numerous in these fibers.
What kind of muscles have slow twitch fibers?
Slow-twitch muscle fibers are resistant to fatigue and concentrated on prolonged, delicate movements and postural control. Compared to fast-twitch fibers, they are more aerobic and have more mitochondria and myoglobin. Because of their blood supply, type I or red fibers are another name for slow-twitch fibers.
Since they have a lot of mitochondria, slow-twitch muscles are aerobic muscles, which means they utilise oxygen to produce ATP, the energy currency. If you have enough oxygen, they can sustain you.
The majority of your muscles are composed of a combination of slow- and fast-twitch muscle fibers. However, the soleus muscle in your lower leg and the muscles in your back that are responsible for maintaining your posture primarily consist of slow-twitch muscle fibers. Additionally, quick twitch muscle fibers make up the muscles that move your eyes.
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Match the following major signs and/or symptoms with a particular source of a food-borne or water-borne illness. Nausea; muscle pain; fatigue; facial swelling, especially around eyes Norovirus Clostridium botulinum Campylobacter jejuni Abdominal cramps, diarrhea (can be bloody), fever, and vomiting Stomach flu Blurry vision, difficulty swallowing, and general muscle weakness Sore throat and violent coughing Giardia Trichinella "Explosive" vomiting and watery, non bloody diarrhea Diarrhea, abdominal pain, weight loss Frequent urination
The below reference image shows the matching of all major signs and symptoms with a particular source of a food-borne or water-borne illness.
The most likely to be contaminated are raw foods of animal origin, namely raw meat and poultry, raw eggs, unpasteurized milk, and raw shellfish. When manure is used to fertilize produce in the field or dirty water is used to wash the produce, fruits and vegetables can also get polluted with animal feces. The toxins generated by growing bacteria, the toxins produced by dangerous algae species, the contaminating of Foodborne illness and/or water with specific bacteria, viruses, or parasites, or a combination of these factors may result in food- and water-borne diseases.
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The ancient remains of several human bodies have been discovered and legally need to be returned to the tribe that contains the closest descendents. You have been called as a witness in the resulting legal case to give your opinion. The jury is confused about the analysis you have done. Could you please explain it to them again? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. ResetHelp restriction map DNA extraction compared the sample to modern DNA samples DNA fingerprint VNTRs gel electrophoresis hybridization to a probe Southern blot polymerase chain reaction chromosome walking restriction enzymes controls 1. After obtaining the samples (preserved bone and muscle fragments), I conducted a ___________. 2. Because I suspected the ancient DNA would be degraded, I pretreated it with random primers and the Klenow fragment (a procedure found to help repair damage and aid amplification) and amplified the DNA using __________. 3. To make sure that the DNA I analyzed was indeed from the ancient sample, during these procedures, I used both positive and negative __________. 4. Following amplification, I used__________ to cut out the DNA fragments with the genes I wanted to study. 5. Then I used _____________to separate the DNA fragments. 6. I performed a ___________, in which I transferred the DNA onto a filter and then incubated the filter with a radiolabeled DNA sequence complementary to the gene I was interested in. 7. This final step with the radiolabeled DNA sequence is called_____________ . I can repeat this step for multiple genes. 8. I used several highly variable loci to create a specific ______________. 9. Finally, I___________ in order to determine the group most closely related to the individual who the sample was taken from.
To modern DNA samples DNA fingerprint VNTRs gel electrophoresis hybridization to a probe Southern blot polymerase chain reaction chromosome walking restriction enzymes controls DNA fragmentation.
1. After obtaining the samples (preserved bone and muscle fragments ) I conducted a DNA extraction.
2. Because I suspected the ancient DNA to be degraded, I treated it with random primers and Klenow fragments and amplified the DNA using a polymerase chain reaction.
3. To make sure the DNA I analyzed was from the ancient sample, during these procedures, I used both positive and negative controls.
4. Following amplification I used restriction enzymes to cut out the DNA fragments with the genes I wanted to study.
5. Then I used gel electrophoresis to separate the DNA fragments.
6. I performed a Southern blot, in which I transferred the DNA onto a filter and then incubated the filter with a radiolabelled DNA sequence complementary to the gene I was interested in.
7. This final step with the radiolabelled DNA is called hybridization to a probe. I can repeat this step for multiple genes.
8. I used several highly variable loci to create a specific DNA fingerprint.
9. Finally, I compared the sample to modern DNA samples in order to determine the group most closely related to the individual who the sample was taken from.
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