Your advisor has given you a tube of GMP that has been sitting in the freezer for many years and wants to know the concentration. There is one problem: the spectrophotometer in the lab has been 'optimized' for use with proteins and only measures at 280 nm. You decide to give it a try. After getting and absorbance reading of 0.2, what is the concentration of GMP in the tube (assuming a 1 cm path length).

Answers

Answer 1

Answer:

2.22 × 10^-5M

Explanation:

From Beer-Lambert's law;

A=εcl

Where A = absorbance of GMP= 0.2

ε= molar extinction coefficient of GMP = 9000 M-1cm-1

c= concentration of GMP= the unknown

l= path length = 1 cm

c= A/εl = 0.2/ 9000 M-1cm-1 × 1 cm

c= 2.22 × 10^-5M


Related Questions

A non polar covalent bond (pure covalent) would form in which of the following pairs of atoms?
A.) H-Cl
B.) Se-Br
C.) Na-Cl
D.) Br-Br

Answers

The answer is Br-Br

Help PlS AFAP and thank you so much

Answers

the answer is B transfer thru direct contact

75 g of a metal is heated to a temperature of 99C. The metal is then placed in a calorimeter containing 145 g of water at a temperature of 25C. The temperature of the water in the calorimeter increase to a final temperature of 28C. What is the specific heat of the metal?

Answers

Answer:

0.34 J / g ⁰C

Explanation:

Let the specific heat of metal be s .

heat will be lost by hot metal and gained by water .

heat lost by metal = mass x specific heat x loss of temperature

= 75 x s x ( 99 - 28 ) = 5325 s

heat gained by water = mass x specific heat x gain of temperature

= 145 x 4.18 x ( 28 - 25 ) = 1818.3

heat lost = heat gained

5325 s = 1818.3

s = 0.34 J / g ⁰C

The sulfuric acid–catalyzed reaction of isopentyl alcohol with acetic acid to form isopentyl acetate is performed. This reaction does not proceed to completion. After the reaction is heated under reflux for an hour, the product mixture is washed with aqueous sodium bicarbonate solution, and the organic layer is thoroughly dried. A distillation of the organic layer is then performed. Given the boiling points listed below, select the compound that will distill first.
Compound Boiling point, ℃
acetic acid 118
isopentyl acetate 142
isopentyl alcohol 130
sulfuric acid water 290

Answers

Answer:

The correct option is acetic acid

Explanation:

Distillation is the process of separating a mixture of substances based on differences in boiling points. During distillation, the compound with the lowest/least boiling point is distilled and collected first and then the one with the next least boiling point and it goes on like that.

From the explanation above, acetic acid has the least boiling point (in the organic layer) with 118°C and thus will distill first. This is then followed by isopentyl alcohol (130°C) and then isopentyl acetate (142°C) and finally sulfuric acid water (290°C).

Galium is a metal that can melt in your hand at 302.93 k. what is the temperature in celcius ?

Answers

Explanation:

subtract it into 273.15to convert it in to celcius

Answer quick (image)

Answers

Answer:

The answer is boiling point

g If the pressure of each gas is increased at constant temperature until condensation occurs, which gas will condense at the lowest pressure

Answers

The question is incomplete; the complete question is;

If the pressure of each gas is increased at constant temperature until condensation occurs, which gas will condense at the lowest pressure?

A) Octane

B) Ethane

C) Butane

D) All the gases will condense at the same pressure

Answer:

Octane

Explanation:

If we study the table closely we will realize that octane has the greatest molecular mass among all the gases shown.

The greater the molecular mass, the lower the pressure required for the condensation of the gas. Hence, the lowest pressure is required for the condensation of octane at constant temperature.

For the combustion of methane, CH4(g) + 2 O2(g) → CO2(g) + 2 H2O(l) △H = -213
kcal/mol

How many kilocalories of energy are released from 5.0 mol of methane?
How many moles of methane are needed to give 100. kcal heat?

Answers

Answer:

1.-213(kcal/mol)*5mol=1065kcal

2.100/213 mol

a. The energy released from 5.0 mol of methane is -1065 kcal/mol.

b. The number of moles of methane needed to give 100. kcal heat is 0.4694 moles.

What is combustion?

Combustion is a chemical process in which the element reacts with oxygen to produce heat and light. Burning of wood.

The equation of combustion of methane:

CH₄ (g) + 2O₂(g) ------> CO₂ (g) + 2H₂O (l)

a) Enthalpy of the reaction is H = -213 Kcal/mol

This means 213 Kcal heat is released in the burning of one mole of CH4

H reaction = [ H products ] - [ H reactants ]

Hr = [ H(CO₂)g + 2H(H₂O)l ] - [ H(CH₄)g ] = -213 kcal/mol

For 5 moles of methane:

5CH₄(g) + 10O₂(g) -----> 5CO₂(g) + 10H₂O(l)

therefore, new Hr = 5 x -213 = -1065 kcal/mol

H reaction = -1065 kcal/mol

b) 213 kcal is released per mole of Methane

then, to release 100 kcal we need, 100 / (213) = 0.4694 moles

Thus, the energy released is -1065 kcal/mol, and the number of moles is 0.4694 moles.

Learn more about combustion, here:

https://brainly.com/question/15117038

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what is the relationship between netforce and acceleration based on the situation?​

Answers

Answer:

The net force is greater at the second situation as well as the acceleration due to the contribution of the second guy.

Explanation:

Hello.

In this case, since the net force is computed via adding all the exerted forces on a body, for the first situation we can see that the net force equals the force provided by that guy when pulling, whereas in the second situation the net force equals the force of both of the guys pulling the car:

[tex]F_N_1=F_1\\\\F_N_2=F_1+F_2[/tex]

For the acceleration, we can see that applying an extra force (due to the second guy) turns out into a greater acceleration, that is why the car at the second situation reaches the tree whereas the other one does not.

Best regards.

What is the velocity of a wave?

A. How much energy the wave has
B. How quickly the wave travels
C. The distance between two wave peaks
D. The distance between the high and low points of the wave

Answers

The answer is b. How quickly the wave travels

which group of substances are elements?
A.Air,water,cooking oil
B.carbondioxide,water,table salt
C.carbon,hydrogen,oxygen
D.rock,soil,iron ore​

Answers

Answer:

C

Explanation:

(A+B) are compounds and D is mixtures

When does a single-displacement reaction occur?
A. When a substance, usually containing carbon, reacts with oxygen
to produce energy in the form of heat and light
B. When one element replaces another element in a compound
during a chemical reaction
C. When a substance is broken down into two or more simpler
substances in a chemical reaction
D. When a single element forms two double elements

Answers

Answer:

B. When one element replaces another element in a compound during a chemical reaction

If you are given 22.990 g of sodium and 12.011 g of carbon, which sample do you expect to have more particles?

Answers

Answer:

they are expected to have the same particles because their masses are the same with there molar mass

When an atom is trying to obtain stability, what part(s) of the atom change(s)?

Answers

Answer: If the outer shell is filled, the atom is stable. Atoms with unfilled outer shells are unstable, and will usually form chemical bonds with other atoms to achieve stability.

(hope this helps ^^)

Neurons contain which two structures that help them transmit messages?

sarcomeres

dendrites

an extracellular matrix

axons

Answers

Answer:

dendrites

axons

Explanation:

got it right on edg.

Answer:

B and D

Explanation:

edge

10. What is the change in the boiling point of water at 1000 C per Pa change under atmospheric pressure conditions? The molar enthalpy of vaporization is 40.69 kJ mol-1, the molar volume of liquid water is 0.019 x 10-3 m3 mol-1, and the molar volume of steam is 30.199 x 10-3m3 mol-1, all at 1000 C and 1.01325 bar. (Hint: change in temperature per change in pressure)

Answers

Answer:

The answer  is  [tex]\frac{dT}{dP} = 2.84*10^{-4}\ K/Pa[/tex]

Explanation:

From the question we are told that

  The boiling point of water is  [tex]T_b = 100^oC = 100 + 273 = 373 \ K[/tex]

  The enthalpy of vaporization is  [tex]\Delta H_{vap} = 40.69 \ kJ mol-1 = 40.69 *10^{3} \ J/mol[/tex]

  The molar volume of liquid water is  [tex]V =0.019 * 10^{-3} m^3 mol^{-1}[/tex]

   The molar volume of steam is  [tex]V_s = 30.199 * 10^{-3} \cddot m^3\cdot mol^{-1}[/tex]

  The pressure is  [tex]P = 1.01325 \ bar[/tex]

Gnerally from Clausius Clapeyron equation we have that

      [tex]\frac{dP}{dT} = \frac{\Delta H_{vap}}{ T * \Delta V}[/tex]

Here  [tex]\Delta V = V_s - V[/tex]

=>     [tex]\Delta V = [30.199 * 10^{-3} ] - [ 0.019 * 10^{-3}][/tex]

=>     [tex]\Delta V = [30.199 * 10^{-3} ] - [ 0.019 * 10^{-3}][/tex]      

=>     [tex]\Delta V = 0.03018 \ m^3 mol^{-1}[/tex]          

So

    [tex]\frac{dP}{dT} = \frac{40.69 *10^{3} }{ 373 * 0.03018}[/tex]

=>  [tex]\frac{dP}{dT} = 3522.28 \ Pa/K[/tex]

Generally from the we are ask to obtain the change in the boiling point of water at 1000 C per Pa change under atmospheric pressure conditions which is mathematically represented as

       [tex]\frac{d T}{dP}[/tex]

So

    [tex]\frac{dT}{dP} = \frac{1}{\frac{dP}{dT} } = \frac{1}{3522.28}[/tex]

=> [tex]\frac{dT}{dP} = \frac{1}{\frac{dP}{dT} } = 2.84*10^{-4}\ K/Pa[/tex]

At what time was the frog sitting still or at rest

Answers

Answer:

frogs don't spend all their time swimming, nor do they spend all ... The frog is capable of sitting perfectly still for hours on end, only to suddenly ... When a frog is resting or hunting, he will sit on whatever is handy.

Explanation:

Hope I helped if I did please mark me brainiest Thanks! <333 :3

Is the moons rate rotation that same as earths

Answers

Answer:

no

Explanation:

Answer:no

Explanation:The moon orbits the Earth once every 27.322 days. It also takes approximately 27 days for the moon to rotate once on its axis.

As the atomic number of elements increases across a period on the periodic table, the atomic radius

Answers

Answer:

Decreases  

Explanation:

Write the equation for the dehydration of the CoCl2 hydrate

Answers

Answer:CoCI2+6H20
......hope this helped

What factor is mostly responsible for the form of matter?
time
temperature
pressure
weather

Answers

Temperature is mostly responsible for the form of matter. For instance, when raising the temperature you're adding energy. This energy gives the individual molecules and atoms the ability to overcome the forces that hold them together. This can be seen when a solid turns into a liquid or a liquid turns into a gas. However, when you're lowering the temperature you're removing energy. The lack of energy results in less movement from the atoms. Gases condense into liquid because the atoms don't have enough energy to stay away from each other. Liquids solidify because the atoms don't have enough energy to slide past each other and they get stuck in place. So in conclusion, temperature determines how how much energy atoms have that allow them to move at different speeds which determines the state of matter.

Chemical equation to show cdso4 dissolves in water

Answers

no se bb si quieres salimos tu sabes

A person drops two objects from the same height. one object weighs 15 N and the other weighs 10 N
A The 10 N object has the same mass as the 15 N object
B The 15 N object has twice the mass of the 10 N object
C The 15 N object has more mass than the 10 N object
D The 10 N object has more mass than the 10 N object

Answers

C) The 15N object has more mass than the 10N object

PWEASE REVIEW ME AND ME MATES TEEF

Answers

So the first one is yellow and the other one is white

Match the descriptions below with the graphs. Be sure to explain your answers.

Answers

There are no graphs

what is the volume in cubic meter and in cubic decimeter of 2.0mol of nitrogen on gas at 1000c and 100,000pa? (R=8.31j/molk)​

Answers

Given parameters:

Number of moles of Nitrogen = 2.0mole

Temperature of the gas = 1000c

Pressure on the gas = 100000Pa

Gas constant R = 8.31J/molK = 0.082atmdm³mol⁻¹k⁻¹

Unknown:

Volume in cubic meter and cubic decimeter = ?

Solution:

The ideal gas law is given as;

       PV  = nRT

where P is the pressure on the gas

          V is the volume of the gas

         n is the number of moles

         R is the gas constant

         T is the temperature of the gas

Let us convert to appropriate units;

      1000°C to K;

       K  = 1000 + 273  = 1273K

100000Pa to atm;

           101325Pa  = 1atm

           100000Pa = 0.99atm

 Input the parameters and solve for the unknown;

      0.99 x V  = 2 x 0.082 x 1273

                   V  = 210.9dm³

to m³;

        1000dm³   = 1m³

        210.9dm³ = [tex]\frac{210.9}{1000}[/tex]   = 0.21m³

A hypothesis must be testable. Which hypothesis is testable? *

Ablue is the best color.
BSummer is nicer than fall.
CDogs are better than cats.
DA beagle can jump higher than a Persian cat.

Answers

the answer is D because can be tested the others ones is opinion

please mark me as brainliest

WILL mark Brainliest

Answers

Answer:

Ok what question

Explanation:

uhh what question do you need help with?

A balloon containing methane gas has a volume of 1.03 L at 99.0 °C . What volume will the balloon occupy at 198 °C ?

Answers

Answer:

The balloon will occupy a volume of 1.32L.

Explanation:

Given the following data;

Original Volume, V1 = 1.03L

Original Temperature, T1 = 99°C

New Temperature, T2 = 198°C

To find new volume V2, we would use Charles' law.

Charles states that when the pressure of an ideal gas is kept constant, the volume of the gas is directly proportional to the absolute temperature of the gas.

Mathematically, Charles is given by;

[tex] VT = K[/tex]

[tex] \frac{V1}{T1} = \frac{V2}{T2}[/tex]

Converting T1, from Celsius to Kelvin = 273 + 99 = 372K

Converting, T2, from Celsius to Kelvin = 273 + 198 = 471K

[tex] \frac{V1}{T1} = \frac{V2}{T2}[/tex]

Making V2 as the subject formula, we have;

[tex] V_{2}= \frac{V1}{T1} * T_{2}[/tex]

[tex] V_{2}= \frac{1.03}{372} * 471[/tex]

[tex] V_{2}= 0.0028 * 471[/tex]

V2 = 1.32L

Therefore, the balloon will occupy a volume of 1.32L at 198°C

Liquid hexane will react with gaseous oxygen to produce gaseous carbon dioxide and gaseous water . Suppose 17. g of hexane is mixed with 101. g of oxygen. Calculate the maximum mass of carbon dioxide that could be produced by the chemical reaction. Be sure your answer has the correct number of significant digits.

Answers

Answer:

52g of carbon dioxide

Explanation:

Liquid hexane + gaseous oxygen --> Carbon dioxide + gaseous water

The balance chemical equation is given as;

C6H12 + 9O2 --> 6CO2 + 6H2O

From the stochiometry of the reaction;

1 mol of hexane (86g) would consume 9 mol of oxygen (144g) to form 4 mol of carbon dioxide (264g)

The limiting reactant in this equation is hexane as it would run out before oxygen. This means hexane determines how much of the carbon dioxide is formed.

86g of hexane = 264g of crbon dioxide

17g of hexane = xg of carbon dioxide

x = 17 * 264 / 86 =  52g of carbon dioxide

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