Why is taq dna polymerase used in pcr reactions rather than a normal dna polymerase?.

Answers

Answer 1

Explanation:

'Taq polymerase ' is thermostable DNA polymerase, a great resistant enzyme isolated from the bacterium Thermus aquatics.

A major limitations of early PCR method was that fresh DNA polymerase has to be added during every cycle and this repetitive step was not just tested but also greatly increased the likelihood of error.

Thermus aquatics is a thermophilic bacterium that can survive temperature up to 95°C so by using Taq polymerase in PCR technique eliminated the need to add fresh polymerase during every cycle.


Related Questions

Which of the following is a basic type of vaccine?
Select all that apply.
a. nucleic acid vaccines
b. subunit vaccine
c. live, attenuated vaccine
d. antitoxin vaccines

Answers

The basic type of vaccines are:

nucleic acid vaccines

subunit vaccine

live, attenuated vaccine

An easy, secure, and reliable method of preventing hazardous infections before you are exposed to them is vaccination. It boosts your immune system and builds up your body's natural defenses against particular illnesses.

Your immune system is trained by vaccinations to produce antibodies, exactly as it does when it is exposed to a disease. However, because vaccinations only include dead or weakened versions of bacteria or viruses, they do not really cause the disease or increase your chance of developing its symptoms.

In order to create immunity, vaccines act in conjunction with your body's natural defenses. Your immune system reacts when you receive a vaccination.

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What part of the circulatory system is responsible both for disposing of cellular waste and providing cellular fuel?.

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The lungs are responsible both for disposing of cellular waste and providing cellular fuel.

To obtain oxygen, the circulatory system (cardiovascular system) pumps blood from the heart to the lungs. The heart then sends oxygenated blood to the rest of the body via arteries. The veins return oxygen-depleted blood to the heart to restart the circulation process. Blood vessels in the circulatory system transport blood away from and towards the heart. Arteries transport blood away from the heart, while veins transport blood back to the heart. The circulatory system transports oxygen, nutrients, and hormones to cells while also removing waste products such as carbon dioxide.

The lungs are an important part of the circulatory system because they provide oxygen for cellular respiration and remove waste products.

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A life cycle in which the only multicellular form is haploid is most typical of __________.

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A life cycle in which the only multicellular form is haploid is most typical of fungi.

In the lifecycle process of fungi, the haploid stage of lifecycle is known to be multicellular and it is te dominant stage.

As a result of the haploid multicellular stage, specialized cells that are also haploid are made.

The process through which these haploid cells will be made is mitosis as for meiosis sexual process has to take place.

After mitosis, the specialized haploid cells that were produced are fused so that a diploid zygote can be formed which will grow into a new fungus.

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infant rats deprived of their mothers' grooming touch produce less growth hormone and have a higher metabolic rate. more growth hormone and have a lower metabolic rate. less growth hormone and have a lower metabolic rate. more growth hormone and have a higher metabolic rate.

Answers

Infant rats deprived of their mothers' grooming touch produce less growth hormone and have a lower metabolic rate (Option C).

What is emotional behavior?

The expression emotional behavior makes reference to the way in which affective issues alter the behavior in humans and also in animals, which in this case is associated with physiological changes such as low hormone production and metabolic rate.

Therefore, with this data, we can see that emotional behavior may produce changes in the physiological conditions not only in humans but also in animals as in this case in rats.

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The ability to produce language is a function of _________, whereas the ability to understand language is a function of _________.

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The ability to produce language is a function of Broca's area, whereas the ability to understand language is a function of Wernicke's area.

What distinguishes Wernicke's territory from Broca's area?

In essence, Wernicke's area strives to ensure that the language makes sense, but Broca's area attempts to guarantee that the language is produced in a fluent manner. The Wernicke-Geschwind model, which was later suggested by neurologist Norman Geschwind, built on this understanding of language.

Broca's area is situated in the neocortex's posterior-inferior frontal convexity, whereas Wernicke's area is restricted to the general region where the parietal, occipital, and temporal lobes converge. For the majority of people, the left hemisphere is where these areas function predominantly in terms of language.

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Explain how a change in breathing rate is a homeostatic mechanism for a person at a higher
altitude.

Answers

An increased breathing rate takes in more oxygen to the lungs and bloodstream to maintain oxygen levels.

what are the molecular and energy products generated by aerobic respiration? a. molecular: 38 atp and heat, energy: 6co2 and 6h2o b. molecular: 6co2 and 6h2o, energy: 38 atp and heat c. molecular: 2 atp, energy: 6o2 and 6h2o d. molecular: 6h2o, energy: heat

Answers

The molecular and energy products generated by aerobic respiration would be [tex]6CO_{2}[/tex], 6[tex]H_{2}O[/tex], an energy of 38 ATP and heat.

So, the correct option is B.

Adenosine triphosphate, a chemical compound that provides energy, is produced as a result of a series of enzyme-controlled chemical reactions in which oxygen reacts with glucose to produce carbon dioxide and water. When oxygen and glucose are combined during aerobic cellular respiration, ATP is created that the cell can use. There are byproducts of carbon dioxide and water. Cellular respiration is an aerobic process in which glucose and oxygen combine to create ATP.

In the second and third stages of the aerobic energy system, the breakdown of glucose that was begun by aerobic glycolysis is continued, producing the byproducts carbon dioxide and water, as well as the synthesis of additional ATP.

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A student is developing a model of sex cell formation.
X
X
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Which of the following conclusions can be made from the student's modol of why the dna of the daughter cells is different from the dna of the parent cell?

Answers

Answer:

Can you please include the answer choices....

Explanation:

Thanks. I'll answer it tho.

select all the anticodons that could bind to the codon ucu for serine. choose all that apply. check all that apply 5'-aga-3' 5'-aga-3' 5'-gga-3' 5'-gga-3' 5'-agg-3' 5'-agg-3' 5'-iga-3' 5'-iga-3' 5'-agi-3' 5'-agi-3' 5'-agu-3' 5'-agu-3' 5'-uga-3' 5'-uga-3'

Answers

All the anticodons that could bind to the codon ucu for serine are 5'-aga-3' 5'-aga-3' 5'-gga-3' 5'-gga-3' 5'-agg-3' 5'-agg-3' 5' as Wobble Hypothesis given by Francis Harry Compton Crick states that 3rd base of mRNA codon can base pair with 1st base of a tRNA anticodon undergoing non-Watson-Crick.

What is Wobble Hypothesis ?

According to the Wobble Hypothesis the first 2 bases of the mRNA codon form Hydrogen bonds with the corresponding bases on tRNA anticodon in Watson-Crick manner.

Through this, they only form the base pairs with the complimentary bases. However, formation of the Hydrogen bonds between 3rd base on codon and 1st base on anticodon can occur potentially in non-Watson-Crick manner.  

Therefore, All the anticodons that could bind to the codon ucu for serine are 5'-aga-3' 5'-aga-3' 5'-gga-3' 5'-gga-3' 5'-agg-3' 5'-agg-3' 5' as Wobble Hypothesis given by Francis Harry Compton Crick states that 3rd base of mRNA codon can base pair with 1st base of a tRNA anticodon undergoing non-Watson-Crick.

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Equation for photosynthesis

Answers

Answer:

Photosynthesis is usually represented by the equation 6 CO2 + 6 H2O + light --> C6H12O6 + 6 O2. During this process, organisms such as plants go through the light-dependent and light-independent reactions to convert carbon dioxide and water into sugars and oxygen.

is tht wht u mean??

6 CO2 + 6 H2O + light --> C6H12O6 + 6 O2

Where are the collateral ganglia?

Answers

Collateral ganglia, also called pre-vertebral ganglia, are situated anterior to the vertebral column and receive inputs from splanchnic nerves as well as central sympathetic neurons. They are associated with controlling organs in the abdominal cavity, and are also considered part of the enteric nervous system

The collateral ganglia are found in abdominal aorta.

What are collateral ganglia?

The prevertebral ganglia, also known as collateral ganglia, are located anterior to the vertebral column and receive input from central sympathetic neurons as well as splanchnic nerves. They are regarded as a component of the enteric nervous system and are connected to the control of abdominal organs.

The sympathetic ganglia known as collateral ganglia, sometimes known as prevertebral ganglia, are located in between the sympathetic chain and the organ of supply. The preganglionic sympathetic fibers that supply the viscera in the abdomen and pelvis relay at this location.

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In the previous question a heterozygous three toed dragon mated with a heterozygous three toes male. What is the probability that their offspring will have three toes?

Question 14 options:

25%


50%


75%

Answers

A heterozygous male three-toed dragon and a heterozygous female three-toed dragon mated. Any offspring of parents who are heterozygous have a 75% probability of being born with three toes.

A dominant allele has a 75% chance of being inherited by the offspring if both parents are heterozygous and afflicted. To find all potential genotypic pairings in the parents, utilize a Punnett square. There are a few crucial differences in a pedigree that shows a dominantly inherited characteristic.

A mating experiment between two organisms equally hybrid for two traits is referred to as a dihybrid cross. A heterozygous organism is one that possesses two distinct alleles at a certain genetic location, making it a hybrid. The traditional Mendelian ratio for a dihybrid cross in which the alleles of two different genes assort independently into gametes is 9:3:3:1, and it can be used to predict the phenotypic ratio.

As a result, we may state that there is a 75% chance that any offspring of heterozygous parents (WW) will have three toes.

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Please help me
It’s due

Answers

The effect of shortage of the following factors on the rate of photosynthesis is given below:

CARBON DIOXIDE (CO₂) - Slows down photosynthesisOXYGEN (O₂) - No changeGLUCOSE (C₆H₁₂O₆) - No changeSUNLIGHT - Slows down photosynthesisWATER (H₂O) - Slows down photosynthesis

What is photosynthesis?

Photosynthesis is the process by which plants produce food from carbon dioxide and water using the energy of sunlight.

The rate of photosynthesis is affected by the following:

amount of sunlight - the greater the amount of sunlight, the faster the rate of photosynthesisamount of carbon dioxide - the greater the amount of carbon dioxide, the faster the rate of photosynthesisamount of water vapor in the atmosphere - the greater the amount of water vapor, the faster the rate of photosynthesis

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when a skeletal muscle fatigues, what happens to the contractile force over time?

Answers

When a skeletal muscle fatigues the contractile force declines due to previous contractile activity. Once the muscle has reached maximum tetanic tension, there is no longer an increase in force generated by the muscle. At this stage, the muscle becomes fatigued and contractile force starts to decrease over time.

Due to earlier contractile activity, skeletal muscle weariness results in a decrease in contractile force. The muscle no longer produces an increase in force after it has stretched to its maximal tetanic tension. At this point, the muscle starts to ache and its contractile force starts to gradually decline. A sign of this kind is muscular weariness, which over time reduces the muscles' capacity for work. Sleepiness is only one symptom of fatigue. It may be difficult to get out of bed in the morning due to fatigue. Additionally, it makes it difficult for someone to do everyday duties. Physical exhaustion and mental fatigue are the two main categories of fatigue.

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Explain your understanding of a calorie, how energy is stored in the body and what factors make our individual energy requirements so unique. A portion of our consumed energy goes towards physical activity each day. Share some of the daily activities you perform that you think would require this energy.

Answers

Calorie, a unit of energy, is the amount of energy expelled when the body breaks down food, it is stored in the body as glycogen or fat and gender, body weight, and age make individual energy requirements unique.

Some daily activities that require physical energy are Riding a bicycle, walking fast, hiking, skating. dancing, workouts.

How are calories burned?

Calories are energy people get from foods and drink they consume. Apart from basic metabolism, the way the body uses food determines how much calories the body burns.

The body stores this energy as glycogen or fat. Body weight, age and gender are determinants of the body's use of energy. Energy requiring activities include workouts, dancing, hiking etc., to burn calories.

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a farmer performs a cross between two species of flowers and expects a distribution of red, pink, and white flowers. out of flowers, the following numbers of red, pink, and white flowers were observed. calculate the expected number of each type of flower.

Answers

Due of the flowers' inability to consistently produce the necessary 1:2:1 ratio of bloom colors, the farmer will be unable to meet demand. There are 250, 500, and 250.

A common flower is what?

The most widespread and well-liked flower genus in the world is undoubtedly the rose. There are over least 360 species of flowers in the Rosa genus, which includes these blooms. These blooms naturally occur in a variety of hues, including red, pink, apricot, and white.

What kind of flower is lovely?

Rose. The rose is known as the "queen of the garden" since it is thought to be the most flower petal in the entire world. It is one of most well-liked flowers on the planet and is available in a variety of hues and sizes. They are also widely distributed around the world.

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4. what experimental technique was utilized to show that dna replicated by a semiconservative process?

Answers

An experimental demonstration of semiconservative DNA replication was provided by the Meselson and Stahl Experiment.

What is Meselson and Stahl Experiment?An experimental demonstration of semiconservative DNA replication was provided by the Meselson and Stahl Experiment. In order to explore DNA replication, Matthew Meselson and Franklin Stahl carried out an experiment on the fast-diverging E. coli in 1958.In 1958, Matthew Meselson and Franklin Stahl performed an experiment on the rapidly diverging E. coli to investigate DNA replication. CsCl density gradient centrifugation was the method employed by Meselson and Stahl to explore semiconservative replication of DNA.Max Delbrück informed PNAS in May 1958 that the semiconservative method of DNA replication had been discovered by the well-known density labeling studies of Matthew Meselson and Franklin W. Stahl.

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what is microbial antagonism?

Answers

Microbial antagonism is the competition between microbial organisms for food assets and territory. As a result, if one organism out competes the other, this leads to the inhibition of the organism that failed to thrive in the surroundings.

Microbial antagonists have an indirect advantageous effect on plant life due to the fact they lessen the pressure from soil borne pathogens on the crop vegetation. however there is also a brilliant quantity of microorganisms inside the soil, that have a right away high-quality impact on plant increase and health (Somers et al., 2004).

Microbial antagonism continues ordinary microbiota from colonizing a few parts of the human body. Microbial antagonism takes place whilst members of the regular microbiota prevent pathogens from colonizing and turning into mounted within the frame.

Antagonism, in ecology, an affiliation among organisms in which one benefits at the expense of the opposite. As existence has evolved, herbal choice has favoured organisms which can be capable of correctly extract electricity and nutrients from their surroundings.

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in the carbohydrate-insulin model, what is the primary cause of obesity?

Answers

Obesity occurs from insulin resistance when lipids become over abundant and an existing storage of lipid layer is unable to be used by the body for energy and is therefore stored and continues to increase with the more food consumed

Which genotypes are possible from a cross of parents with the genotypes dd x dd?.

Answers

The genotypes possible from cross of parents with genotypes dd×dd will be dd and dd.

Genotype may be defined as the biological constituent of a living being or the genes which combine to form an organism. The genotype determines all the properties of the loving organism including its appearance, color and all other hereditary features as well. Any organism that is produced due to sexual interaction between parents will have both the genes coming from the parent. The cross formation of genotypes can be explained with the help of dihybrid cross. It says that since each parent has four different combinations in the gametes so there are 16 possibilities for the cross. In the question the cross is between dd×dd. So if we take one gene from each parent then the genotype will be dd and dd which is the required genotype.

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one kind of plasmid in bacteria is called an r plasmid. what kinds of genes are typically present on such plasmids?

Answers

One type of bacterial plasmid is called an r-plasmid. Types of genes typically present in such plasmids Antibiotic resistance genes

Antibiotic resistance genes are often carried in plasmids or transposons and are transferred from cell to cell by conjugation, transformation, or transduction. This gene exchange allows rapid spread of resistance within bacterial populations and between different bacterial species. Some genetically modified plants contain genes that make the plant resistant to certain antibiotics. Scientists often then and can say that add these resistance genes during genetic modification. This allows GM plants and cells to be distinguished from non-GM plants and cells.

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What is the purpose of microtubules during Mitosis?

Answers

The main purpose of microtubles during Mitosis is responsible for the separation and distribution of chromosomes to daughter cells.

Which statement best explains the function of the vesicles?
Select one:
O Delivering packaged materials to the Golgi apparatus for protein
synthesis
O Transporting packaged molecules from the Golgi apparatus to
be released out of the cell
O Exchanging genetic information between the Golgi apparatuses
of separate cells
Extracting portions of the Golgi apparatus to be regenerated for
growth within the cell

Answers

The statement that best explains the function of vesicles is: (b) Transporting packaged molecules from the Golgi apparatus to be released out of the cell.

Vesicles are the membrane enclosed spherical structures that are involved in the process of transportation. The vesicles transport  packaged proteins and lipids from the Golgi body to lysosomes, ER or cell membrane. These vesicles are coated with certain protein that direct the pathway for the transport.

Golgi apparatus is a single membranous cell organelle. It is involved in the packaging, sorting and transport of proteins and lipids. The structure of Golgi is composed of cisternae and vacuoles.

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What alleles show their effect even if the individual only has one copy of the allele?.

Answers

Even if an individual only carries one copy of an allele, dominant alleles still have an effect.

Even if an individual only possesses one copy of an allele, dominant alleles still have an impact. One copy of the "brown eye" allele, for instance, is all that is required to have brown eyes because the "brown eye" allele is dominant (although, with two copies you will still have brown eyes).

One copy of an allele, which might come from just one parent, is called a dominant allele and results in a dominant phenotype in people. Having two copies of a recessive gene, one from each parent, is necessary for a recessive phenotype to develop. The dominant phenotype of a gene is seen in an individual who carries both one dominant and one recessive allele.

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perspective that our genes and environmental influences work together to determine our characteristics

Answers

Environmental factors often influence traits independently of genes. Either of these effects can change the proteins that are made from a gene, which in turn affects traits.

How do environment and gene determine our personality?

Some genes increase the characteristic and others work to decrease the same characteristic-complex relationship among the various genes, as well as a variety of random factors, produce the final outcome.

Because of their genetic makeup, individuals differ in their responsiveness to the qualities of the environment. There are unique, genetically influenced reactions to particular experiences to which we are exposed.

The presence of drugs and chemicals in an organism's environment can also influence gene expression in the organism. The environment can affect morphological and physiological development whereas genes influence morphology and physiology, creating a framework within which the environment acts to shape the behavior of an individual.

Hence in this way, genes and environmental influences work together to determine our characteristics.

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The cell membrane pulls away from the cell wall as the cell loses water and shrivels during.

Answers

Answer: plasmolysis

Explanation:n a hypertonic solution, a cell with a cell wall will lose water too

Iconic maine species such as moose, canada lynx, loons, boreal chickadees, eastern brook trout, saltmarsh sparrows and atlantic puffins are under stress from climate change. A mutation in the boreal chickadees increases the ability of the bird to catch and eat certain insects. Which factor might determine whether the frequency of the new allele will increase?.

Answers

The frequency of the new allele may be determined by whether the mutation offers the chickadees better access to food sources than those without it.

Mutation:

A mutation is a permanent change in the genetic material (the genome) of a cell of a live creature or of a virus that may be passed on to the cell's or virus's progeny. (Organic genomes are all made of DNA, although viral genomes can be made of either DNA or RNA; see heredity. The Physical Basis of Heredity.) A mutation in the DNA of a body cell of a multicellular organism (somatic mutation) can be passed on to descendant cells via DNA replication, resulting in a sector or patch of cells with abnormal function, such as cancer.

Mutations occur as a result of errors during the regular chemical transactions of DNA, most commonly during replication, or as a result of exposure to high-energy electromagnetic radiation (e.g., ultraviolet light or X-rays), particle radiation, or highly reactive substances in the environment. Mutations are believed to be largely harmful since they are random alterations, although some may be advantageous in specific settings. In general, the mutation is the primary source of genetic variation, which serves as the raw material for natural selection evolution.

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sustainable development must be considered during the developmental activities justify this statement​

Answers

Sustainable development has been defined as development that meets the needs of the present without compromising the ability of future generations to meet their own needs

Hope this helps:)

Earth has several nutrient cycles that share similarities, but also differ. Connect each feature of a cycle that relates to the phosphorus, nitrogen, or carbon cycle. You may use cycles, and the features of the cycles, more than one time each.

Answers

Excess present in fertilizers can upset the balance of the cycle---- Nitrogen and phosphorus cycles.

Mostly in a form unusable by organisms--- nitrogen

Present in soil and organisms ---- carbon and nitrogen

Involved in respiration---- carbon

Fixed by bacteria from the atmosphere----- nitrogen

Often deposited on the ocean floor--- phosphorus

Excess present in the atmosphere can upset the balance of the cycle--- carbon.

How are the phosphorus, nitrogen, and carbon cycles interconnected?

Every living organism is made up of atoms of carbon, nitrogen, and phosphorus. Nitrogen and carbon are present in amino acids which are responsible for making proteins. Phosphates form DNA and ATP molecules. The availability of these elements has great importance to the existence of living things.

Nitrogen Cycle is a process in which nitrogen is converted into other forms that are passing from the atmosphere to the soil, then to organisms, and back into the atmosphere. It has several processes such as nitrogen fixation, nitrification, and denitrification, etc.

The carbon Cycle is a process of circulation of carbon through biotic and abiotic parts of the ecosystem. It is a type of gaseous cycle. Phosphorus Cycle is a process of circulation of phosphorus through biotic and abiotic parts of the ecosystem. It is considered a sedimentary cycle.

So we can conclude that the phosphorus, nitrogen, and carbon cycles are interconnected to each other because they are important for the survival of the organism.

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PLSSS HELP IF YOU TURLY KNOW THISS

Answers

The Monkey because he is consuming/eating the Pear
Monkey is the only consumer….
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