Which of the following statements regarding restriction enzymes is NOT true?
A) Most restriction enzymes recognize sequences of 20-25 bp in length.
B) Bacteria can add methyl groups to their DNA to prevent digestion by endogenous restriction enzymes.
C) Most restriction enzymes recognize palindromic sequences in host DNA.
D) Restriction enzymes may produce either sticky or blunt ends when digesting host DNA.

Answers

Answer 1

Answer:

The correct option is option A

Explanation:

Restriction enzymes are endocleases that cleave DNA fragment (of usually four, five or six nucleotide long) at specific sites to produce blunt or sticky ends. They recognize palindromic sequences of host DNA when cleaving the specific sites. The sequences below (on complementary strands) give an example of a palindromic sequences.

5'-CCC║GGG-3'

3'-GGG║CCC-5'

As can be seen above, when read from 5' to 3', the two sequences are the same despite being on opposing strands. And when cut between the guanine (G) and cytosine (C) (as shown above), it produces a blunt end. But when cut as shown below produces a sticky end.

5'- G║AATTC -3'

3'- CTTAA║G -5'

The explanation above shows options C and D are right while option A is wrong (hence the correct option).

Also, bacteria prevent their own DNA from been digested by restriction enzymes by adding methyl group to their restriction sites which prevents restriction enzymes from recognizing restriction sites of their DNA; this generally makes bacterial DNA to be highly methylated. This explanation makes option B right also.


Related Questions

true or false Geoengineering will have no negative effects on the environment.

Answers

The answer is false

It is false that geoengineering will have no negative effects on the environment.

What is geoengineering ?

Geoengineering, also known as Climate Engineering, is the deliberate, extensive modification of the Earth's environment and climate in order to stop further climate change, halt and stop global warming, and lessen the effects on human society and the ecosystem.

Spraying compounds into the atmosphere to create reflecting aerosols and reflect sunlight into space is known as solar geoengineering. Although this would prevent global temperatures from rising, local or regional temperatures may still rise over the coming years, according to a recent study.

Hence, it is false that geoengineering will have no negative effects on the environment.

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How many elements in nitrogen monoxide?

Answers

Answer:

Two elements

Explanation:

Nitrogen and Oxygen only, (NO)

Can you help me please i will give you a branlist and it’s science

Answers

Answer:

B.

Explanation:

I think it's because protons are the ones that circle, neutrons stay in the middle becuz they're neutral, and electron are negatively charged?

A person’s will be the same on Jupiter and Earth. A person’s will be greater on Jupiter than on Earth.

Answers

Answer:

Greater on Jupiter

Explanation:

Gravity depends on mass. Jupiter has more mass than Earth so its gravity is two and a half times greater. Therefore a person will weigh more on Jupiter.

Answer:

A person will be greater on Jupiter than Earth. The surface gravity is having is larger than Earth’s by roughly 2.4 times. So, if you weigh 100 pounds on Earth it would be 240 on Jupiter. Hopefully this helps! Feel free to mark this as brainliest!  

Explain the formation of sedimentary rocks?​

Answers

sedimentary rocks are types of rocks that are formed by the accumulation  or deposition of small particles and subsequent cementation of mineral or organical particles on the floor of oceans or other bodies  of water at the earths surface

Explanation:

Sedimentary rocks form when it’s deposited out of air ice wind gravity. It’s basically formed when erosion breaks down into a rock in an area

A 7.2 × 10–5 C charge has an electric potential energy of 1.08 × 10–2 J. The electric potential, to the nearest whole number, is V.

Answers

Answer:

150.

Explanation:

On edge

The electric potential, to the nearest whole number is 150 V.

What is the electric potential energy?

The electric potential energy denoted by U is proportional to the charge and inversely proportional to the distance between the point where its is brought from infinity to the charge.

U = kQ₁Q₂/r

For identical charge,

U =kQ²/r................(1)

and the electric potential is given by

V = kQ /r................(2)

Dividing both the equations, we get

U/V  = Q

Substitute charge Q =  7.2 × 10⁻⁵ C, Electric potential energy U = 1.08 × 10⁻² J, then the electric potential will be

V  = 1.08 × 10⁻² /   7.2 × 10⁻⁵

V = 150 Volts

Thus, the electric potential is 150 Volts.

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Which of the following is true of food webs?
a. Food webs don't actually exist in nature.
b. A food web shows the flow of decomposers
from producers to consumers and, finally, to
producers.
c. In food webs, detritivores break down organic
waste to return nutrients to the ecosystem.
d. When diagramming food chains and food
webs, the arrows should be drawn from the
lower trophic levels to the higher levels to
show the flow of energy.
e. As energy is moved up the food web, most of it
is conserved by the species in the next trophic
levels.

Answers

Answer:E

Explanation:

Is this picture below a compound or an element?

Answers

Glucose is a compound.


Glucose is both a molecule AND a covalent compound. Glucose is made up of three elements: carbon, hydrogen, and oxygen.

The Murray River can be found in: South, America ,Europe, Africa ,Australia or Asia? :>

Answers

Hey there!

ANSWER:

South Australia

EXPLANATION:

Where can the Murray river be found?

The Murray River is located in South Australia. Since that's where it's located, you can find it there.

Hope this helps!

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Why fatty acids need a protein transporter to move cross membrane?

Answers

Answer:

Transport of long-chain fatty ácids across the cell membrane has long been thought to occur by passive diffusion........Fatty ácids transporters hold promise as a future target to rectify lipid fluxes in the body and regain metabolic homeostasis.

Sue has Parkinson's disease. She takes medication that increases the level of

Answers

Answer:

Sue has Parkinson's disease. She takes medication that increases the level of dopamine.

Explanation:

You catch, tag, and release 35 fish back into a pond. Then, you catch 70 more fish, 12 of which have tags on them. What is the best estimate of the number of fish in the pond?
a.
100
b.
150
c.
142
d.
204

Answers

Answer:

D. 204

Explanation:

proportion : 12/70 = 35/x

70(35)=12x

2450=12x

204.166667=x and round it to make it 204.

The best estimate of the number of fish in the pond is 204. Thus, option D is correct.

Calculation:

proportion : 12/70 = 35/x

70(35)=12x

2450=12x

204.166667=x and round it to make it 204.

What is average speed?

Average speed has been defined as the total distance travelled in total time taken it means that we can calculate the average speed obtained by an object .

Mathematically,

Average Speed = Total distance travelled/Total time taken

It's S.I unit is meter/second

Average speed is a scalar quantity it means that it has only magnitude not direction.

Ecosystem has been the branch of science that deals with the study of relationship between living organisms and natural phenomena. Ecosystem has the system in which both living as well as nonliving organisms are present in a particular location.

Green plants contain chlorophyll and they prepare there own food in the presence of sunlight and the process is known as photosynthesis. In the process of photosynthesis light  energy converted into chemical energy.

Therefore, The best estimate of the number of fish in the pond is 204. Thus, option D is correct.

Calculation:

proportion : 12/70 = 35/x

70(35)=12x

2450=12x

204.166667=x and round it to make it 204.

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when a sediment is transported down a mountain and along a stream, we are seeing the conversation of

Answers

Answer:

The options are

A. density B.volume C. area D.matter

The answer is Matter

Explanation:

When a sediment is transported down a mountain and along a stream, we are seeing the conversation of Matter.

The sediments transport substances which have a mass and they occupy different positions and volumes. This is the main reason why the answer is known as Matter.

What are the roles of the two test
groups in this
investigation?
A. Both are experimental groups, and each
Individually shows the effect of the direction
B. Dhe is the experimental group and one is the
control group, and together they show the
ellect of the direction of light.
C. Both are control groups, but only Test Group)
shows the effect of the direction of light.
D. Both are experimental groups, but only Test
Group 1 shows the effect of the direction of
light

Answers

Answer:

We would also need to see the investigation to be able to help sorry

There are 12 apples 14 oranges and 16 bananas in a fruit basket.what is the ratio apples to bananas

Answers

Answer:

12:16

Explanation:

Which type of feed is typically fed to baby chicks?
crumbles
mash
pellets
scratch

Answers

I feed mine a started mix and that usually has pellets in it.

Explain why circulatory problems often occur with people who are bedridden or with inactive people who seldom use their muscles.

Answers

Good luck :))))))))))))

what is the differences between boiling and autoclaving​

Answers

Explanation: Water baths reach a maximum boiling point of 100°C (212°F). Autoclaves ideally operate at higher temperatures of 120°C (284°F) and can go as high as 133°C (273 °F) Boiling in a water bath can take an hour or longer for effective sterilization to be achieved.

Select all answers that reflect contributions molecular approaches have made to modern taxonomy. Check All That Apply revealed that ssu rRNA is highly conserved so can be used for classificationrevealed that ssu rRNA is highly conserved so can be used for classification have led to the development of a new taxonomic system divided into domainshave led to the development of a new taxonomic system divided into domains have not changed the overall tree of lifehave not changed the overall tree of life revealed that archaea are more closely related to prokaryotic than eukaryotic cells

Answers

Answer:

- revealed that rRNA is highly conserved so can be used for classification

- have led to the development of a new taxonomic system divided into domains

Explanation:

The ribosomal RNA (rRNA) genes are widely used in evolutionary, phylogenetic, and phylogeographic studies as molecular markers because they represent nuclear genes that are highly conserved between species. However, it is important to highlight that rRNA genes form arrays at specific chromosomal sites, thereby they often exhibit pseudogenes that may interfere with their use as molecular markers (i.e., they produce multiple bands in the electrophoresis gel). In 1977, Carl Woese showed that Archaea is an evolutionary lineage separated from Bacteria by using molecular evidence based on an rRNA molecular marker (16S rRNA). Thus, Woese indicated that Eukarya, Bacteria and Archaea represent three different domains that arose separately from an evolutionary ancestor named 'progenote'.

According to erikson theory which stage of social development is related to adulthood?

CHOICES: A. Trust vs. Mistrust
B. Intimacy vs. Isolation
C. Initiative vs. Guilt
D. Autonomy vs. Shame​

Answers

Answer:b

Explanation:

Enzymes are biological catalysts. Enzymes are proteins that are found inside cells to
increase the rate of chemical reactions within each cell. Enzymes are denatured
(destroyed) by various environmental conditions. Amylase is an enzyme in the digestive
system that helps to break down complex carbohydrates into simple sugars.
HYPOTHESIS: Amylase works best in an environment with a pH level of 7. what is the independent and dependent variable sum1 please help!

Answers

Answer:

Explanation:

independent= pH as you will change the pH in order to test your hypothesis and see if it's true

dependent= how amylase works-needs to be measured so you can see how easily it does it function depending on the pH (dependent on the independent variable)

The independent variable is pH, you change it to test your hypothesis; The dependent variable is the activity of amylase enzyme depend on the pH value.

What are the properties of an enzyme ?

Enzymes are protein catalysts that speed up the rate of biochemical reactions

The  enzyme activity is pH-specific, catalyze both forward and reverse reaction but do not decide the direction of the biochemical pathway.

An enzyme have active site which bind to substrate  and form desired products.

Several factors influence the enzyme activity like heat, temperature and varying pH.

Enzyme show absolute, relative, group, stereo-specificities and regulatory function.

Physical properties include enzyme act as colloid, it become inactivated below boiling point of water.

Enzymes are  thermos-labile as it can withstand temperatures of 100 degrees.

The optimum temperature needed for enzymatic activity varies from 25 degrees Celsius to 45 degrees Celsius.

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What's an example of a monosaccaride?

Answers

Answer:

Glucose

Explanation:

Any simple sugar, like glucose, is a monosaccaride.

Camille was excited when the new instrument showed up at the lab. She
opened it up and started using it right away. What was her error?
A.) He used an inappropriate instrument.
B.) He used an uncalibrated instrument.
C.) He misread a measurement.
D.) He used the incorrect units.

Answers

Answer:

I would say B- it was uncalibrated

Explanation:

he did not take the time to make sure it was calibrated before use

When a liquid changes to a gas the process is called?

Answers

Answer:

Condensation

Explanation:

the word is called condensation

A biomass pyramid of an ecosystem is shown.
Tertiary consumers
Secondary consumers
Primary consumers
Producers
Which statement provides the best explanation for the difference in biomass of organisms found at each trophic level?
A. Organisms at higher trophic levels have less energy available to them than organisms at lower trophic levels.
B. Organisms at higher trophic levels require smaller habitats than organisms at lower trophic levels.
c. Organisms at lower trophic levels provide less energy than organisms at higher trophic levels.
D. Organisms at lower trophic levels outcompete organisms at higher trophic levels.

Answers

Answer:A

Explanation:

The statement provides the best explanation for the difference in biomass of organisms found at each trophic level is: organisms at higher trophic levels have less energy available to them than organisms at lower trophic levels.

WHAT IS TROPHIC LEVEL:Trophic level of an organism refers to the position of the organism in the flow of energy in an ecosystem.

Organisms feed on one another in an ecosystem to obtain energy needed for their metabolic activities.

More energy is contained in lower trophic levels than that of higher trophic levels. This means that, according to this question, more energy is in the producers than tertiary consumers.

Therefore, the statement provides the best explanation for the difference in biomass of organisms found at each trophic level is: organisms at higher trophic levels have less energy available to them than organisms at lower trophic levels.

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What happens to a gas when heat is removed?

A. The particles have more kinetic energy.

B. The particles move faster and become less compact.

C. The particles move slower until the matter changes into a. liquid.

D. The particles reduce their size by at least half.

Answers

Answer C ) The particles move slower until the matter changes into a liquid
It’ll be C! Hope this helped :))

how new technology and experimental methods can lead to changes in theories.

Answers

Answer:

New findings and new evidence about a  scientific theory can change the aspects of this scientific theory and in some cases, it can be proved as wrong or can be supported by more strong evidence that can prove its liability.

New technology allows scientists and researchers to study various untouched aspects and leads to find new evidence to support or prove the theory can be either change or unproven.

Thus, new technology and experimental methods can lead to changes in theories.

Leigh has collected data and performed statistical
tests for analysis. Describe the conclusions she can
draw about the data based on her analysis.

Answers

Answer:

She will obtain results to support -or reject- her working hypothesis. These results are subjected to statistical analyses in order to confirm that they are statistically significant

Explanation:

The scientific method is a rigorous process that consists of raising a working hypothesis, which is tested by experimentation or observational procedures and analysis of the results. The statistical analyses may have key roles during this procedure. In an experiment that involves statistical analyses, the results are subjected to statistical measures to evaluate the significance level and thus confirm (or reject) the statements made in the working hypothesis. The p-value is one of the most used statistical measures to determine if differences are due to randomness, or they are statistically significant.

Answer:

Because the calculated chi-square value of 5.19 is less than the critical value of 7.82, the null hypothesis can be supported. She is 95% confident that there is no significant variation in shell color along the beach coastline.

Explanation:

this is the Sample Response

A block of wood with a density of 10g/cm^3 is cut in half. What would the
expected density of the two halves be? *
A The density would double.

B The density would halved.

C The density would remain the same.

D The density would increase by a quarter.

Answers

the answer to ur problem above is (b)

What ocean characteristics does sonar help to study?​

Answers

Answer:

Sonar, short for Sound Navigation and Ranging, is useful for investigating and planning the sea since sound waves travel farther in the water than do radar and light waves

Explanation:

Other Questions
50 POINTS FOR THE ANSWER! Vanessa and Zack are playing a game where the player with the lower score wins. At the end of the game, Vanessa has a score of Negative 3 and StartFraction 5 over 8 EndFraction and Zack has a score of Negative 3 and two-thirds. Which statement explains who won? Comparing the decimal equivalents of their scores shows that Vanessa won because 3.625 is less than Negative 3.ModifyingAbove 6 with bar. Comparing the decimal equivalents of their scores shows that Vanessa won becauseNegative 3.ModifyingAbove 6 with bar is less than 3.625. Comparing the decimal equivalents of their scores shows that Zack won because 3.625 is less than Negative 3.ModifyingAbove 6 with bar. Comparing the decimal equivalents of their scores shows that Zack won becauseNegative 3.ModifyingAbove 6 with bar is less than 3.625. what is 660 increased by 1 followed by a further one how many numbers lie between squares of 12 and 13 Solve for w.4 = 0.25(w-4.3) While location is about how to find something, ___ is about ___ Rewrite 8 3/4 as an improper fraction.A. 35/4 B. 28/3 C.35/8D.32/4 Which term represents 0 latitude? North Pole international date line equator prime meridian 2. Is the Voynich manuscript written in some kind of code? (D2+6d+9)+(d3-6d+9) Simplify: 6 (4x - 5) Seth, Pete, Cara, and Jen form Kingfisher Corporation with the following consideration:Consideration Transferred Basis to Transferor Fair Market Value Number of Shares IssuedFrom SethInventory $30,000 $96,000 30*From PeteEquipment ($30,000 of depreciation taken by Pete in prior years)45,000 99,000 30**From CaraProprietary process 15,000 90,000 30From JenCash 30,000 30,000 10*Seth receives $6,000 in cash in addition to the 30 shares.**Pete receives $9,000 in cash in addition to the 30 shares.a. Seths recognized gain or loss. Identify the nature of any such gain or loss.b. Seths basis in the Kingfisher Corporation stock.c. Kingfisher Corporations basis in the inventory.d. Petes recognized gain or loss. Identify the nature of any such gain or loss.e. Petes basis in the Kingfisher Corporation stock.f. Kingfisher Corporations basis in the equipment.g. Caras recognized gain or loss.h. Caras basis in the Kingfisher Corporation stock.i. Kingfisher Corporations basis in the proprietary process.j. Jens recognized gain or loss.k. Jens basis in the Kingfisher stock. 6*10^-2 is how many times as large as 2*10^-8 10. Write and equivalent expression for theexpression 4-4. 2. Elements in the same group have similar properties because they have O the same number of protons O the same number of valence electrons O the same number of energy levels with electrons O the same total number of electrons please help!! Two sides of a triangle, a and b, have lengths 1 in Jackie is often surprised when she goes to other countries to learn that people do business so differently. For instance, in meetings Jackie is used to getting straight to the point and addressing any relevant issues. However, she has learned that in many countries businesspeople take the time to first make small talk and ask about the family before finally getting down to business. To Jackie this seems to make the meetings unnecessarily long and wastes valuable time. These cultural differences seem strange to Jackie because she is relying on her:________ a. self-reference principle b. international bias c. self-reference criterion d. cultural relativism e. ethical perspectives Sex chromosome disorders are often not recognized until A coordinate plane with a line passing through (negative 3, 2) and (0, 3) Find the linear function represented by the graph. The slope of the line is . The y-intercept of the line is at . What linear function is represented by the graph? What is a unit rate? Use context clues to determine the meaning of the underlined words in this passage