When the gene encoding a certain cytoskeleton protein is deleted, the resulting mutant cells round up and do not form their normal appendages. These mutants can be rescued when a gene encoding an N-terminal green fluorescent protein (GFP) fusion of the protein is expressed, but not when a gene encoding a C-terminal GFP fusion is expressed. Which fusion protein is appropriate to use in studying cellular localization and activity

Answers

Answer 1

Answer: N-terminal green fluorescent protein (GFP)

Explanation:

Protein synthesis takes place in ribosomes located in the cell cytoplasm, in which amino acids are transported by transfer RNA corresponding to each amino acid to the messenger RNA where they bind in the appropriate position to form new proteins. The messenger RNA has a sequence of nucleotides that are translated into protein, as each codon (set of three amino acids) codes for one amino acid.

Genes are the storage units of genetic information, so they are segments of DNA that contain the information on how the cells of the organism should function. Each gene codes for a protein, so if a gene is damaged or absent, the protein will not be obtained. In this case, mutant cells with a deleted gene, will round up and wont form their normal appendages. This mutation can be rescued or repaired with a gene that encodes an N-terminal green fluorescent protein (GFP) fusion of the protein. The N-terminal end is the amino-terminal end and it refers to the end of a protein that ends with an amino acid that has a free amino group. The C-terminus or carboxyl-terminus is the end of a protein that ends in a carboxyl group. The convention for writing peptides is to place the C-terminal end to the right and write the sequence from the N- to the C-terminal end. So, when an N-terminal green fluorescent protein (GFP) fusion of the protein is expressed, the gene will produce the protein with a GFP tag.

GFP is Green Fluorescent Protein, and it is a protein produced by the jellyfish Aequorea victoria that emits bioluminescence in the green region of the visible spectrum. When a gene is fused to another gene (at either the N- or C-terminus, although in this case it is the N-terminus), the entire messenger RNA is translated together as if it were a single fused protein. Thereby, since the protein will be produce with a GFP tag, it can be seen under the microscope and it will be apropiate to use in studying cellular localization and activity.

There are some considerations that this problem does not question, such as that there must be a start codon for protein synthesis and a stop codon, and this stop codon must not be in the middle of the gene or between the gene and the GFP tag.


Related Questions

I need to know what is the answer for number 6 and 7 and that’s all

Answers

Answer: Both are correct

Explanation:

Pls help I’m failing

Answers

Answer:

C.

Explanation:

Not A. because it didn't mention that (and it's not true)

Not B. because that wasn't mentioned (and common sense tells you it incorrect)

C. because it was mentioned (differently) and it made perfect sense

Not D. because it didn't even talk about how many letters (or sounds) there was to be true (plus look at A. above)

True or false: Desertification is a natural process caused by natural events. ANSWER QUICK

Answers

Answer:

true?

Explanation:

Rabbits in most areas have a brown coat. The arctic hare has a bright white coat in
winter. How does the arctic hare's white color help it survive?

Answers

Answer:

Indeed, rabbits, in most of the planet, tend to have their fur colored brown, copper or other similar shades. Now, in the Arctic regions, these animals do not have this color, but a white one, similar to the environment that surrounds them. This is so because these animals, for evolutionary reasons, have adapted their fur to the snowy environment that surrounds them in the Arctic, in order to avoid being detected by potential predators, protecting themselves and trying to ensure their survival.

5.) What is the process by which the egg is released called?

6.) When does ovulation occur?

Answers

Answer:

5.) What is the process by which the egg is released called?

A.) Ovulation

6.) When does ovulation occur?

A.) Ovulation typically occurs about 14 days before the start of the next menstrual period.

-TheUnknownScientist

PLEASE HELP FAST!!!!

As humans continued to burn more and more oil and natural resources what has happened
to the world's carbon dioxide output?

Answers

Answer:

Global warming 100%  one of them.  And Air pollution.

Explanation:

Good luck

please helppp i’ll mark u the brain thingggg !!!!!!

Answers

Answer:

The correct answers are:

1. A. eukaryote 2. B prokaryote 3. A. animals 4. B bacteria

Explanation:

The cell illustrated in A is a eukaryotic cell as it has a membrane-bound nucleus and organelles. The nucleus contains chromatin in the denser area in the center of the nucleus called the nucleolus. An example of this type of cell is animals or plants.

The other cell is a prokaryotic cell as it has no cell organelle or true nucleus. It has a denser area in cell plasm called nucleoid that has chromosome present. The example of these cells are bacteria or archeae.

Which can be used to prevent initial entry of bacteria into the body?

Answers

Answer:

safeguard

Explanation:

safeguard sabon na skalam

Inflammation can be used to prevent initial entry of bacteria into the body.

what name is given to an enzyme which catalyses' the breakdown of proteins?

Answers

Answer:

Proteases

Explanation:

Pro - protein

eases - enzyme

A particular species of bacteria has a "a generation time" of 20 minutes. That is, every 20 minutes a single bacterium completes prokaryotic fission -- one bacterium is now two bacterial cells. This morning at 6:00 a.m. you made a sandwich and packed your lunchbox. What you were not aware of was that there were 10 bacteria cells of this species on the dollop of mayonnaise that you spread across your bread. At 12:00 p.m. (no on) You sit down and eat your entire sandwich! How many bacteria cells did you have for lunch? (Hint: use the formula 2axb x C = numbers of cells, where "a" - number of times per hour the bacteria reproduces, "5" the number of hours the bacteria had to reproduce, and "C" = the number of bacteria at the start).​

Answers

Answer:

Number of bacteria cells = 2621440 cells

Explanation:

The generation time of a bacteria specie is the time it takes for a population of bacteria to double in number. The generation time for most common bacteria is quite short, about 20 minutes to 1 hour.

Bacteria reproduce asexually by binary fission, in which the parent cell divides into two to produce two identical daughter cells.

In the given question, the generation time of the bacterial species is 20 minutes.

The time that have passed from between the time the sandwich was packed at 6:00 am to lunch time by 12:00 pm is 6 hours.

Using the given formula in the hint: number of cells = 2^(ab) × C

Where a = number of times per hour the bacteria reproduces; b = number of hours; C = number of bacteria at the start

a = 60 minutes/ 20 minutes = 3; b = 6; C = 10

Number of cells = 2^(3 × 6) × 10

Number of cells = 2621440 cells

The technology time for the maximum not unusual place bacteria is pretty short, approximately 20 mins to one hour. The number of bacteria cells did have for lunch =2621440 cells.

Bacteria reproduce via way of means of binary fission, wherein the discern molecular divides into a time of the bacterial species is 20 mins.The time which has handed from among the time the sandwich became packed at 6:00 am to lunchtime via way of means of 12:00 pm is 6 hours.Using the given formulation withinside the hint: range of cells =2^  (ab)×C.Where a = range of instances in step with hour the reproduces; b = nu of hours; C = number of bacteria on the start.a = 60 mins/ 20 mins  = 3; b = 6; C =10.The number of bacteria cells did have for lunch =2621440 cells.

What is bacterial cel?

Bacterial cells are exceptional from plant and animal cells. Bacteria are prokaryotes, because of this that they haven't any nucleus. A bacterial includes Capsule.

Thus it is clearly explained above that the number of bacteria cells did have for lunch =2621440 cells.

To learn more about the bacteria refer to link :

https://brainly.com/question/17817006

Starting with the European settlers, humans have introduced earthworms from Europe and Asia into North American forests. These introductions continue through the transport of soil that contains non-native earthworms, such as during construction and through the release of non-native earthworms used for fishing. The effects of non-native earthworms are especially large in forests that did not have any native earthworms. For example, forests of the Great Lakes region did not previously have earthworms until humans introduced them. When non-native earthworms are introduced, the thick layer of leaf litter disappears quickly, thereby altering biogeochemical cycles. You conduct an experiment to compare the nitrogen cycle in soils with and without non-native earthworms. Predict the results of your experiment.

Answers

Answer:

The soils with earthworms will show a faster rate of ammonification

Explanation:

Originally, the nitrogen released by organisms when they die (or excrete waste products) is organic nitrogen, e.i., amino acids and nitrogenous bases in DNA. Ammonification is the property that decomposer organisms have to mineralize organic nitrogen in order to produce inorganic nitrogen in the form of ammonium (NH4+). In agroecosystems, earthworm activity can increase the ammonification rate. For example, Lumbricus terrestris is an invasive earthworm (which is native to Europe) that has been shown to increase both ammonification and nitrification (nitrate production) rates in the soil of different ecosystems.

PLEASE HELP !! ILL GIVE 40 POINTS ; PLUS BRAINLIEST !! DONT SKIP ANSWER.

Answers

B. Selective breeding. Humans have been choosing certain dog breeds to make other breeds. The Labrador retriever is an example of humans creating different breeds for certain tasks

Where do most transform boundaries occur?

Answers

Answer:

QUESTION:

Where do most transform boundaries occur?

ANSWER:

Transform Plate Boundaries are locations where two plates slide past one another. The fracture zone that forms a transform plate boundary is known as a transform fault. Most transform faults are found in the ocean basin and connect offsets in the mid-ocean ridges.

Explanation:

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• 1. What is the difference between a gamete and a zygote?
• 2. Are gametes haploid or diploid? Are zygotes haploid or diploid?
• 3. What occurs at fertilization?
• 4. Define diploid and haploid.
• 5. What characterizes sexual reproduction?
• 6. What characterizes asexual reproduction?
• 7. What type of reproduction occurs in prokaryotes?
• 8. What is somatic tissue? What is germ-line tissue?
• 9. What do somatic cell lines and germ-cell producing cell lines have in common? What makes them different?
• 10. What happens in each stage of Meiosis I and Meiosis I1?
• 11. How does each stage of Meiosis compare to Mitosis?
• 12. Describe three ways that meiosis contributes to genetic diversity in a species?

Answers

Answer:

Explanation:

A gamete is a haploid reproductive cell that fuses with another gamete of opposite strain to form a diploid

zygote.

A zygote is a cell formed by the fusion of the male and female gamete before it undergoes division.

gametes are haploid while a zygote is diploid.

There is exchange of genetic material during sexual reproduction and fusion of gametes. Meiosis is also involved.

The opposite occurs in asexual reproduction and mitosis is involved instead.

Asexual reproduction is common in prokaryotes eg bacteria.

where the input molecules of respiration come from

Answers

Answer:

see the explanation

Explanation:

Most of the steps of cellular respiration take place in the mitochondria. Oxygen and glucose are both reactants in the process of cellular respiration. The main product of cellular respiration is ATP; waste products include carbon dioxide and water.

What is the importance of mitosis for unicellular organisms?
A) asexual reproduction
B) sexual reproduction
C) repair of damaged tissue
D) growth

Answers

Answer:

A) asexual reproduction

Explanation:

Mitosis plays an important part in the life cycle of most living things, though to varying extents. In unicellular organisms such as bacteria, mitosis is a type of asexual reproduction, making identical copies of a single cell. In multicellular organisms, mitosis produces more cells for growth and repair.

Answer:

hello again

Explanation:

motosis play an important

Identify the labeled structures

Answers

Answer:

what labeled structures?

Explanation:

help its due this Wednesday

Answers

Answer:

first food chain:

The Producer: the trees

The Primary Consumers: elephants

The Secondary Consumers: the cheetah

The Scavengers: the termites

The Decomposers or Detritivores: mushrooms

second food chain

The Producer: grass

The Primary Consumers: zebras

The Secondary Consumers: the hyena

The Scavengers: the vultures

The Decomposers or Detritivores: microorganisms

the food web:

The Producers - the trees and grass.

The Primary Consumers – the zebras and elephants.

The Secondary Consumers – the cheetah, hyena.

The Scavengers –  the termites, vultures

The Decomposers or Detritivores – mushrooms microorganisms.

If the amount of primary Consumers increases, the number of producers would decrease and the number of other consumers would increase, but after a short time their number would also decrease and it causes immigrations.

Also, there's a nice picture I have found on a site;

Please me help fast!!

Answers

Answer: you can just press sumbit for plato

Explanation:

s

m/forms/d/e/1FAIpQLSf1siktOoZRkggNo-AzHPw62091WglmEyyJt30t8yy3LbzpVQ/viewform
15. When two similar unicellular organisms fuse and exchange nuclear materials,
it is known as
O A. Haploidy
B. Conjugation
O .
C. Meiosis
D. Nuclear growth​

Answers

The answer would be C

Meiosis

Have a great Saturday

Certain prehistoric events led to the formation of oil. Which of the following was one of those events? A giant wind storms destroy forests. B glaciers melted at the north and south poles . C Life in the sea died and was buried under sediment. D heavy flooring soaked the earths crust.

Answers

Answer:

C oil is just dead animal remains

Explanation:

Answer: C

Explanation: ,

HELP ME PLEASE ? Helppppppppppp

Answers

Let me get a quick answer

What principle is used in the above diagram to calculate the amount of carbon dioxide that is absorbed by land?

Answers

Answer:

The law of conservation of mass

Explanation:

In physics and chemistry, the law of conservation of mass or principle of mass conservation states that for any system closed to all transfers of matter and energy, the mass of the system must remain constant over time, as the system's mass cannot change, so the quantity can neither be added nor be removed.

rho = density

t = time

nabla = divergence

v = flow velocity field

Describe the characteristics of the keratinocyte membrane that restrict water movement across the membrane. If isolated keratinocytes are placed in a hypotonic solution for 24 hours, explain why there are likely to be far fewer cells at the end of this time than there were at the start.

Answers

Answer:

It has four layers and attach with each other with desmosome.

Explanation:

The keratinocytes are arranged in four different layers which are the stratum basale, stratum spinosum, stratum granulosum, and stratum corneum. These cells are attached with desmosome which is a membrane protein. The presence of these four layers made a barrier against damage done by heat, UV radiation, water loss, pathogens etc. There are fewer cells at the end of the time as compared to the start because may be of the water pressure on the membrane or these cells for a long time.

a. DNA replication produces two identical DNA molecules, called ________which separate during mitosis.
b. After chromosomes condense, the __________ is the region where the identical DNA molecules are most tightly attached to each other.
c. During mitosis, microtubules attach to chromosome; at the ____________
d. Ir dividing cells, most Of the cell's growth Occurs during ___________

Answers

Answer:

a. sister chromatids

b. histone

c. metaphase plate

d. interphase (if not a choice, then prophase)

Who wrote about the idea that if more people are born then die it can lead 1 point
to war, famine, and will slow population growth.*

-Hutton

-Lamarck

-Malthus

-Farmers/Breeders

Answers

Malthus was the guy who wrote this

At the end of Meiosis II, each daughter cell has 23 single-stranded chromosomes.
True
OR
False

Answers

Answer:

y the end of meiosis, the resulting reproductive cells, or gametes, each have 23 genetically unique chromosomes. ... Each daughter cell is haploid, because it has half the number of chromosomes as the original parent cell.

Explanation:  

30 chromosomes

Each daughter cell will have 30 chromosomes. At the end of meiosis II, each cell (i.e., gamete) would have half the original number of chromosomes, that is, 15 chromosomes. 2.

From a diploid germ cell 2n =24 double stranded chromosomes, meiosis will produce four gamete haploid cells n =12 single stranded chromosomes.

Meiosis produces four with half because double stranded chromosomes represents DNA replication. Twice as much DNA as a diploid cell needs.

From a diploid germ cell 2n =24 double stranded chromosomes, mitosis will produce two diploid daughter cells 2n =24 single stranded chromosomes for the same reason.

DNA replication doubles the genome before cell division. Sister chromatids of double stranded chromosomes represent the replicated DNA. During cell division, sister chromatids of double stranded chromosomes are separated into different cells.

Meiosis has two stages, the first separating homologous pairs of double stranded chromosomes, and then the second stage separating sister chromatids into four haploid (n) gametes.

Betelgeuse is hotter than Spica.
True or false?

Answers

Answer:

False

Explanation:

Betelgeuse is roughly 3x less colder than Spica.

A test tube is filled with water and a green plant called Elodea. When the plant is exposed to sunlight, it begins to produce bubbles. What is the most likely conclusion for the identity of the gas in the bubbles? Explain.

Answers

Answer:

The correct answer is - oxygen.

Explanation:

The experiment of a test tube and elodea plant in which a plant of elodea is placed in a test tube filled with water and exposed to the sunlight and one is placed in the dark. The plant that is exposed to the sunlight starts forming a gas that can be seen as bubbles in the test tube.

This experiment shows the process of photosynthesis that converts sunlight into glucose with the help of carbon dioxide and water and produces oxygen as a byproduct that releases from the plants.

Thus, the gas that forms in the test tube in the form of bubble is oxygen which is released in the process of photosynthesis.

What is the correct equation for the breakdown of ozone? please answer quick

Answers

Answer:    This electricity converts the oxygen molecule to ozone. Our equation is as follows: 3O2 + energy = 2O3.

The correct equation for the breakdown of ozone can typically occur in a series of two steps known as the destruction of ozone. It is illustrated thus;

Cl + O3 ⇒ ClO + O2ClO + O ⇒ Cl + O2

The net reaction of the above process is the reaction of an oxygen atom with an ozone particle to form two molecules of oxygen.

The catalyst in this reaction is chlorine because it undergoes changes as the process continues while ozone is removed in the process. In the presence of sunlight, atomic oxygen is formed.

This entire process is referred to as the destruction of ozone.

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