what is the minimum voltage required to generate h2 and o2 if the pressure on the gases at the depth of the wreckage (2 mi ) is 300 atm ?

Answers

Answer 1

A. 7 x 10^8 mole of H2 requires 8.48 x 10^10 Coulombs of electrical charge, and a voltage of 632.2 V. The minimum electrical energy required to raise the Titanic is 4.02 x 10^13 kW hr, and the minimum cost is $9.2 x 10^7.

1. The amount of H2 gas required to buoy the Titanic is 7 x 10^8 mole.

2. The number of Coulombs of electrical charge required to generate this amount of H2 gas is computed using Faraday's Law:

Q = nF

Where n is the number of moles of H2, F is the Faraday constant (96485 C/mol), and Q is the number of Coulombs.

Therefore, Q = (7 x 10^8) x (96485 C/mol) = 8.48 x 10^10 C.

3. The minimum voltage required to generate H2 and O2 gases is computed using the Nernst Equation:

E = E° - (RT/nF) ln(Q/Q°)

Where E is the voltage, E° is the standard voltage (1.229 V at 300 atm and 25°C), R is the gas constant (8.314 J/molK), T is the temperature (288 K at 2 miles depth), n is the number of moles of H2, F is the Faraday constant (96485 C/mol), Q is the number of Coulombs, and Q° is the standard charge (1 C).

Therefore, E = 1.229 - (8.314 J/molK x 288 K / (7 x 10^8 mol x 96485 C/mol)) ln(8.48 x 10^10 C / 1 C) = 632.2 V.

4. The minimum electrical energy required to raise the Titanic is calculated using the equation:

E = QV

Where E is the electrical energy, Q is the number of Coulombs, and V is the voltage.

Therefore, E = (8.48 x 10^10 C) x (632.2 V) = 4.02 x 10^13 kW hr.

5. The minimum cost of the electrical energy required to generate the necessary H2 gas is calculated using the equation:

Cost = (E x Price) / (1000 x 3600)

Where E is the electrical energy, Price is the cost per kW hr, and 1000 and 3600 are conversion factors.

Therefore, Cost = (4.02 x 10^13 kW hr x $0.23/kW hr) / (1000 kW hr/kW hr x 3600 s/hr) = $9.2 x 10^7.

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Complete question:

Some years ago a unique proposal was made to raise the Titanic. The plan involved placing pontoons within the ship using a surface‐controlled submarine‐type vessel. The pontoons would contain electrodes (cathodes) and would be filled with H2 gas as a result of the electrolysis of water. It has been estimated that it would require about 7 x 10^8 mole of H2 gas to provide the buoyancy to lift the ship

a) How many Coulombs of electrical charge would be required?

b) What is the minimum voltage required to generate H2 and O2 if the pressure on the gases at the depth of the wreckage (2 miles) is 300 atm?

c) What is the minimum electrical energy (in kW hr) required to raise the Titanic by electrolysis?

d) What is the minimum cost of the electrical energy required to generate the necessary H2 gas if the electricity costs $0.23 per kilowatt‐hour (kW hr) to generate at the site?


Related Questions

consider two different isotopes of the same neutral element. which statements about these isotopes are true? (there may be more than one correct choice.)

Answers

The number of protons in each isotopes is the same. Because both isotopes have the same amount of orbital electrons, the claims made about them are accurate.

What does an element mean?

An element is a basic thing that is difficult to divide into smaller parts. A material that cannot be broken down by non-nuclear processes is referred to as an element in chemistry and physics. An element is a unique component of a bigger system or set in computers and mathematics.

What makes anything an element?

Any material that cannot be broken down into simpler compounds by regular chemical processes is a chemical element, often known as an element.

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What information does a molecular formula provide?

A. the number and kind of atoms that are bonded by the transfer of electrons
B. the simplest whole number ration of atoms that are bonded by the transfer of electrons
C. information about a molecule's structure
D. the number and kind of electrons present in a molecule

Answers

The information in which molecular formula helps to provide is the number and kind of electrons present in a molecule.

The correct answer choice is option d.

What is a molecular formula?

A molecular formula is a formula which which helps to give information about the characteristic feature of an molecules as to the number and kind of electrons present in it.

A molecule is a substance which can normally exist alone and still retain the chemical properties of that substance.

In conclusion, we can now confirm from the explanation given above that molecular formula helps to to understand the constituent of a molecular substance.

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2. In the equilibrium between carbonic acid, bicarbonate, and carbonate which
molecule(s) are favored at low pH?

Answers

There would be more of the bicarbonate ion present in the solution at low pH.

What specie is favored at low pH?

We know that the pH has to do with the degree of the acidity of the alkalinity of a given solution. We know that carbonic acid can be able to dissociate in solution via a reversible reaction.

In this case, when the pH is low, we know that there would now be more of the bicarbonate ion as the acid would  begin to dissociate so as to counter the constraint of the pH that has been imposed on the system.

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what is one possible problem from regenerating a greensand bed by adding a higher dosage of potassium permanganate?

Answers

The chemical element KMnO4 is the same as potassium permanganate. Manganese is in the +7 oxidation state in this salt. The salt is also referred to as "potash permanganate." The ion of permanganate is a potent oxidizer.

Another popular technique for treating water to remove sulfur is manganese greensand. It is often advised for water with less hydrogen sulfide than 6.0 mg/l. A unique coating on a manganese greensand filter causes hydrogen sulfide gas to oxidize into solid sulfur particles, which are then filtered. Potassium permanganate is used to rejuvenate the greensand after all of the manganese oxide has been used up. Potassium permanganate is a purple oxidizing chemical that is added to the untreated water to maintain the manganese greensand filter.

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if the two different types of hydrogen atoms were equally reactive, what ratio of 1-chloropropane to 2-chloropropane would we expect as monochlorination products?

Answers

It is crucial to be able to extend out a line diagram to a condensed formula, therefore the first step in this process.

The next step is to begin sketching the various scenarios as we switch out one hydrogen on a propane cylinder for a chlorine atom. It should be obvious that there are only two options for chlorinating propane: C-1 or C-2 (chlorination at C-3 would give the same product as that at C-1).

Monochlorination means the addition of a chlorine atom. When chlorine atom is added to the below structure. Chlorine will substitute at one of the hydrogens.

Constitutional isomers of these two substances are 1-chloropropane and 2-chloropropane.

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the combustion of g benzoic acid increases the temperature of a bomb calorimeter by . calculate the heat capacity of this calorimeter.

Answers

With this method, a sample is burned in a bomb calorimeter at a constant volume. Equation q = -CT, where C is the calorimeter's heat capacity and T is the temperature change, can be used to determine how much heat is released during the reaction.

If 1.000 g of benzoic acid burns in a bomb calorimeter to produce 26.38 kJ of heat, 0.550 g of benzoic acid will produce the following amount of heat:Conventionally, heat release is denoted by a negative sign.The sum of the heat emitted by combustion (Qc) and the heat absorbed by the bomb calorimeter (Qb) equals zero, according to the Law of Conservation of Energy.

The temperature of the bomb calorimeter rose from 22.01 °C to 24.27 °C after absorbing 14.5 kJ. Using the following expression, we can determine the calorimeter's (C) heat capacity.

In a bomb calorimeter, the combustion of 0.550 g of benzoic acid raises the temperature from 22.01 °C to 24.27 °C.

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a paricular vitamin's chemical formula has one colat atom. what is the molar mass of the vitamin if it conatain 4.34% cobalt

Answers

You can use the molecular mass of Co with its percentage composition to discover the molar mass of cyanocobalamin is 59.903g.

Atomic mass Co = 58.901 amuPercent cobalt with the aid of using mass = 4.34%To discover the molar mass of 1 percentage of the molecule, divide the atomic mass of Co with the aid of using its percentage of general composition.1 percentage of cyancobalmin = 58.901 amu / 4.34% = 13.5715 amuSo now multiply this with the aid of using 100%1357.15 amuThe molar mass of cyanocobalmin is 1357.15 g/molCheck 4.34 percentage of 1357.15 is 59.903 .

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What process is represented by this redox equation?
-
6H20 + 6CO2
C6H1206 +602
A. Cellular respiration
B. Rusting
C. Photosynthesis
D. Combustion

Answers

The process that is represented by this redox equation

6 H₂O + 6 CO₂ --->  C₆H₁₂O₆ + 6 O₂ is  photosynthesis.

The correct option is C.

What is a redox reaction?

A redox reaction is a reaction in which oxidation and reduction occur simultaneously at the same time and to the same extent.

In a redox reaction, electrons are transferred from one species to another.

The species that transferred electrons becomes oxidized whereas the species that accepts electrons becomes reduced.

Hence, a reduction reaction is a reaction in which a chemical species accepts electrons while an oxidation reaction is a reaction in which a chemical species donates electrons.

Considering the given reaction:

6 H₂O + 6 CO₂ --->  C₆H₁₂O₆ + 6 O₂

In the given reaction, carbon is reduced while oxygen is oxidized.

The reaction shows the process of photosynthesis where carbon dioxide is reduced to produce glucose.

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which of the following geometries for the complex ion [co(en)(h2o)4]2+ are possible?

Answers

Octahedral type of molecular geometries are possible for the complex ion [Co(en)(H2O)4]2+.

In chemistry, an octahedral molecular geometry (also called a square bipyramid) is a compound with six atoms or groups of atoms or ligands arranged symmetrically around a central atom and defining the vertices of the octahedron represents the shape of The octahedron has eight faces, so the prefix is ​​okta. The octahedral shape is characterized by Octahedral bond angle values of 90° and 180° define the octahedral shape. The angles between the four atoms forming the square bases of the two pyramids of the octahedral structure are 90°, as is the angle between each of these atoms and one of the apex atoms.

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Complete question :

Which of the following geometries for the complex ion [Co(en)(H2O)4]2+ are possible?

Check all that apply

trigonal bipyramidal or square pyramidal

octahedral

linear

tetrahedral or square planar

trigonal planar

What volume will 3.21 moles of oxygen gas occupy at stp

Answers

Answer:

71.9 L

Explanation:

PV = nRT

STP means 273K and 1.00 atm

(1.00 atm)(V) = (3.21 mol)(0.08206 L*atm/mol*K)(273K)

V = 71.9 L

Which of the following statements about the nucleus is true?

Answers

Answer:

The nucleus is surrounded by a double unit membrane from all sites. The chromosomes and genes which have genetical characters and transmit from one generation to another are found in the nucleus.

Explanation:

it takes 16.6 min for a 10.0 ml sample of an unknown as to effuse through a pinhole. a 10.0 ml sample of helium, he, required 5.00 min. what is the molecular weight of the unknown gas?

Answers

176.36g is the molecular weight of the unknown gas

At equal volumes, t2/t1= √M2 / √M1

Let the molecular mass of the unknown gas be x

16.6/5 = √x /4

√x = 16.4 × 4 / 5 = 13.28

x = 13.28*13.28

x = 176.36g

The entire mass of the atoms required to form a molecule per mole, expressed in grams, is what makes up the molar mass, also known as the molecular weight. Grams per mole are used to measure molar mass. The mass of 1 mole of a material, expressed in M grams, is its molecular weight, also known as its molar mass, abbreviated M. The mass of a given substance's molecules is its molecular weight. While molar mass and molecular weight have distinct definitions and measurement systems, their values are equivalent.

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a compound is a chemical substance with a given set of properties that cannot be broken down into substances with other properties. true false

Answers

This would be false. Many compounds can be broken down and take on other properties through the means of undergoing a chemical change. Hope this helps. Please give brainliest I need five more.

which of the following is not a ligand to the porphyrin ring fe(ii) ion in oxymyoglobin?

Answers

His E7 isn't a ligand for the porphyrin ring (Fe(II)) ion in oxymyoglobin.

In the porphyrin ring, four pyrrole nitrogens are bound to the a ferrous (Fe(II)) ion centre. The Fe(II) ion does have six coordination sites, four of which are inhabited by pyrrole nitrogen, one by proximal histidine, and the final site can form a reversible bond with an O2 molecule.

A porphyrin is a large ring molecule composed of four pyrroles, which are smaller rings with four carbons and one nitrogen. A series of single and double bonds connect these pyrrole molecules, causing the formation of a large ring. The technical term for four pyrroles linked together is tetrapyrrole. The ring is flat in space and has a fairly even electron distribution around in its circumference.

A porphyrin is thus categorized as an aromatic compound. This demonstrates how stable a porphyrin molecule is. Porphin is the general porphyrin model. This molecule appears as just an intermediate in nature only on rare occasions, but it serves as the foundation for all porphyrin molecules.

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copper (63.546 amu) has two isotopes: 63 cu (62.9396 amu) and 65 cu (64.9278 amu). what percent of copper atoms are 65 cu?

Answers

Isotopes are two or more atom types that share the same atomic number (number of protons in their nuclei), location in the periodic table, and chemical element. Still, they have different nucleon numbers (mass numbers) as a result of having a different number of neutrons in their nuclei. Although the chemical properties of each isotope of a given element are nearly identical, they differ in their atomic weights and physical characteristics.

Along with 27 radioisotopes, copper (29Cu) has two stable isotopes: 63Cu and 65Cu. The radioisotope 67Cu, with a half-life of 61.83 hours, is the most durable; 54Cu, with a half-life of roughly 75 ns, is the least stable. A minute or less is the average half-life.

The percentage of copper atoms in 65 Cu is 25%.

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each drug has chemical and physical properties that must be considered when developing the pharmaceutical formulation, these include:

Answers

That the breakdown or dissolution of the drug after administration constitutes the pharmaceutic phase of drug activity, which must occur in medicine composition.

Describe healthcare.

Healthcare can be simply defined as organised medical care provided to an individual, group, or even community by a physician, nurse, or other qualified healthcare practitioner.

However, medically related Patients may contract infections from bacteria, viruses, fungi, and other pathogens while receiving therapy for another ailment.

For this reason, the primary goal of healthcare is to improve quality of life and healthy living through the provision of high-quality healthcare services.

As a result, the nurse is aware that the pharmaceutic phase of drug action, which must occur after administration of the medication, is the drug's breakdown or dissolution.

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how many moles of naoh are contained in 15.2l of a 0.205m naoh solution? multiple choice 125 moles naoh 0.0134 moles naoh 3.12 moles naoh 74.1 moles naoh 0.0779 moles naoh

Answers

3.12 moles NaOH  are contained in 15.2l of a 0.205m NaOH solution

15.2 x 0.205 = 3.116 moles. By rearranging the equation, you must first determine how many moles are there in this solution. False. Molarity (M) x Volume (L) equals 15.2 x.205, or 3.12 moles.

Molarity (M), which is determined by dividing the solute's mass in moles by the volume of the NaOH  solution in liters, is the most often used unit to represent NaOH solution concentration: liters of solution/moles of solute equals M. One liter of a NaOH  solution with a 1.00 molar concentration (1.00 M) contains 1.00 moles of solute.

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methanol (ch3oh) can be made by the controlled oxidation of methane: ch4(g)+12o2(g)→ch3oh(g)

Answers

The standard Gibbs free energy change for the reaction at 25°C is approximately - 111.158 KJ/mol.

To calculate the standard Gibbs free energy (ΔG°) at 25°C for the reaction CH4(g) + 1/2 O2(g) → CH3OH(g), we can use the formula:

ΔG° = ΔH° - TΔS°

where ΔH° is the standard enthalpy change for the reaction, T is the temperature in kelvin, and ΔS° is the standard entropy change for the reaction.

Assigning the respective values given in the question, we have:

ΔG° = (-126.4 kJ/mol) - (298 K)(-51.1 J/mol*K)

= -126.4 kJ/mol - (-15.2418 KJ/mol)

= - 111.158 KJ/mol

Therefore, the standard Gibbs free energy change for the reaction at 25°C is approximately - 111.158 KJ/mol. This value tells us that the reaction is thermodynamically favorable and will tend to occur spontaneously under standard conditions.

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Complete question:

Methanol, CH3OH, can be made by oxidation of methane in limited quantity.

CH4(g) + 1/2 O2(g) CH3OH(g)

ΔH = -126.4

ΔS = -51.1

Calculate ΔG° at 25°C.

Which one of the following conditions would always result in an increase in the internal energy of a system? A) The system loses heat and does work on the surroundings. B) The system gains heat and does work or the surroundings. C) The system loses heat and has work done on it by the surroundings. D) The system gains heat and has work done on it by the surroundings. E) None of the above is correct

Answers

The system gains heat and has work done on it by the surrounding, this condition would always result in an increase in the internal energy of a system. thus the option D is correct.

When a system is heated or has work done on it by its environment, the internal energy of the system is constantly raised. Energy is an immaterial substance that is concerned with performing work on an item. One of the fundamental conservation rules that holds true throughout the cosmos is the conservation of energy. A type of energy is internal energy. The total kinetic energy of each gas molecule can be considered to be the internal energy U of our system. In other words, when the gas's temperature T rises, its molecules move more quickly and its internal energy U rises.

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a compound contains 27.04% sodium, 16.48% nitrogen and 56.52% oxygen what is the empirical formula of the compound?

Answers

NNaO3  is the empirical formula of the compound, a compound contains 27.04% sodium, 16.48% nitrogen and 56.52% oxygen

⇒Find molar mass of each component: N=14.0067, Na=22.98976928, O=15.9994

⇒Convert to moles: N=1.1765797796769, Na=1.1761753530743, O=3.5295073565259

⇒Find smallest mole value: 1.1761753530743

⇒Divide all components by the smallest value: N=1.0003438489011, Na=1, O=3.0008343120781

⇒Round to closest whole numbers: N=1, Na=1, O=3

⇒Combine to get the empirical formula: NNaO3,

The scientific name for nitrogen is N, and it is an element that has no colour nor smell. The ground beneath our feet, the water we drink, and the air we breathe all contain nitrogen. In reality, nitrogen makes up the majority of the atmosphere on Earth, making up around 78% of the atmosphere.

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in the laboratory you are asked to make a 0.643 m nickel(ii) iodide solution using 355 grams of water. how many grams of nickel(ii) iodide should you add

Answers

The amount of nickel(ii) iodide that should be added for making the solution is 71.3 kilograms.

As the molality and weight of water is given, we will use the formula of molality to find the mass of nickel(ii) iodide.

Molality = number of moles of nickel(ii) iodide/weight of solvent in kilograms

Number of moles = mass/molar mass

Molar mass = 312.5 grams

Keep the values -

0.643 = moles/355

Moles = 228.3

Mass = 228.3 × 312.5

Performing multiplication on Right Hand Side of the equation

Mass = 71,332.8 grams

Mass = 71.3 kilograms

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an aqueous solution at has a h30 concentration of . calculate the concentration. be sure your answer has the correct number of significant digits

Answers

H3O+ is present in aqueous solutions with a concentration of 8.8 1012 M at 25 °C. Determine the amount of OH present. Please include significant digits in your response. OH ([OH]) concentration: Take water's ability to ionize itself as an example. 2 H2O(aq), H3O+(aq), and OH (aq)

Kw = [H3O+] [OH] = 1.0 1014 [OH] = 1.0 1014 / [H3O+][OH] = 1.0 1014 / 8.8 1012 [OH] = 1.1 103 M is the equilibrium constant for this reaction.

How much H3O+ is present in water?

In pure water, there are 10-6mol dm-3 H3O + ions present.

How is the pH of a solution determined using H3O?

The hydronium ion concentration in moles per liter must be known in order to compute the pH of an aqueous solution (molarity). After that, the pH is determined using the formula pH = - log [H3O+].

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how many of the following molecules possess dipole moments? bh3, ch4, pcl5, h2o, hf, h2

Answers

BH3,CH4,PCl5,and H2 have zero dipole moment whereas the H2O and HF have permanent dipole moment.

what is dipole moment?

The bond dipole moment is the moments but property of the chemical bond that is used to measure the polarity of the bond within a molecule.

BH3 a non-polar molecule owing to its symmetric structure.

CH4 forms a regular tetrahedral structure and has very small electronegativity difference between the atoms that cancels out each other due to their directional vectors. Thus CH4 is non polar.

PCl5 is made up of polar P−Cl bonds that form a trigonal bipyramidal molecular which is arranged symmetrically thus the PCl5 molecule is nonpolar.

H2O is a polar compound with a bent shape, which is the reason for the polarity of the compound.

HF is a polar molecule owing to higher electronegativity difference and no other bonds to cancel the polarity thus formed.

H2 is a diatomic molecule and hence it is non-polar.

Thus, the atoms that possess dipole moments are H2O,HF .

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write a chemical equation for formation of a polymer via a condensation reaction from the monomers succinic acid (hoocch2ch2cooh) and ethylenediamine (h2nch2ch2nh2).

Answers

HOOCCH2CH2COOH + H2NCH2CH2NH2 ----> Polymer + H2O is the chemical equation for formation of a polymer via a condensation reaction from the monomers succinic acid (hoocch2ch2cooh) and ethylenediamine (h2nch2ch2nh2).

A condensation reaction is a chemical reaction in which two smaller molecules combine to form a larger molecule, releasing a small molecule such as water or methanol as a byproduct. Condensation reactions are often used to synthesize polymer, which are large molecules made up of repeating units called monomers.

To write a chemical equation for the formation of a polymer via a condensation reaction from the monomers succinic acid and ethylenediamine, we can start by representing the monomers as follows:

Succinic acid: HOOCCH2CH2COOH

Ethylenediamine: H2NCH2CH2NH2

In a condensation reaction, the monomers combine through a dehydration synthesis reaction, in which a molecule of water is released as a byproduct. The resulting polymer consists of repeating units of the monomers linked by a covalent bond.

The general chemical equation for a condensation reaction between two monomers can be written as follows:

Monomer 1 + Monomer 2 --> Polymer + byproduct

In this case, the polymer produced by the condensation reaction between succinic acid and ethylenediamine would be a polyamine with repeating units of the monomers linked by a covalent bond. The chemical equation for the formation of this polymer via a condensation reaction would be:

HOOCCH2CH2COOH + H2NCH2CH2NH2 -> Polymer + H2O

It is important to note that the actual structure of the polymer produced in this reaction will depend on the specific conditions of the reaction and the specific monomers involved. The chemical equation provided is a general representation of a condensation reaction between these monomers and does not represent the exact structure of the polymer that would be produced.

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Which of the following is NOT an ionic compound?
A. AIN
B. CO
C. K20
D. Li2O

Answers

CO is not ionic because both elements in the compound are gases. Therefore the answer is B. Please give brainliest I need five more

what is the concentration, in m/v percent, of a solution prepared from 50. g nacl and 2.5 l of water?

Answers

The concentration, in m/v percent, of a solution prepared from 50. g nacl and 2.5 l of water is 2 %.

The mass/volume percent is the ratio of a solution's total volume to the mass of the solute that makes up that solution. Since this type of concentration has been expressed as a percentage, the given proportion should be multiplied by 100.

The calculation if concentration is shown as:

It can be calculate as follows:

% NaCl = Mass of NaCl / Total mass × 100 %

% NaCl = 50 gram / 2500 × 100 %

%  NaCl = 2 %.

As a result, the solution made from 50 g of nacl and 2.5 l of water will have a 2% concentration.

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what volume of oxygen is produced at stp when 6.58 x 1024 molecules of water is decomposed according to the following reaction?

Answers

122 L of oxygen is produced at STP when water is decomposed to the reactants. here decomposition reaction occurs.

Decomposition Reaction is the process or effect of simplifying a single chemical entity into two or more fragments. Chemical decomposition is usually regarded and defined as the exact opposite of chemical synthesis. Decomposition reaction of water can be written as,

        2H2O  → 2H2 + O2

Oxygen is produced at STP when 6.58 x 1024 molecules of water is decomposed. So there will be,

      0.5 × 6.58 × 1024

     = 3.29 × 1024  O2 molecules.

1mole = 6.02×1023 particles, so the amount produced is:

     = 3.29×1024 / 6.02×1023

     = 5.47mole

At an STP of 273K, 1atm the volume is 22.4L/mole

So the volume of oxygen is 5.47 × 22.4 = 122L

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What is the mass of a neutron in atomic mass units (amu)?

Answers

The mass of a neutron in atomic mass units is 1.008665 amu

Because neutrons are atomic particles, neutrons are a component of atoms. The English physicist James Chadwick discovered neutrons for the first time in 1932. He found the presence of a particle with no electric charge while conducting radioactivity studies, and he named it the neutron which typically has zero charge.

The atomic mass unit or AMU is a unit of mass used to express the masses of atoms and molecules. The mass of a neutron is almost the same as the mass of a proton whereas the electron is much less massive. In terms of AMU, the mass of a neutron in atomic mass units is 1.008665 amu

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HELP ME PLEASE THANK YOU

Answers

Answer: 2.)black

3.)red

4.)yellow

5.) I don't know I am very sorry

6.) I don't know I am very sorry

7.) light blue

8.) purple

9.) dark green

10.)purple

given a concentrated solution of 50x concentration tae buffer, what volume (in ml) of this concentrated solution would we dilute to have 500ml of 1x tae buffer solution?'

Answers

We would dilute 10mL of this concentrated solution to make 500ml of a 1x TAE buffer solution.

Dilution is one of the easiest ways to make buffers. This is only applicable, though, if the working solution, which is the desired solution, has the same pH as the stock solution, which is the readily available, more concentrated solution. The dilution equation is employed in the preparation of buffer by dilution:

[tex]C_{1} V_{1} = C_{2} V_{2}[/tex]

where V refers to volume and C represents concentration. The subscripts 1 and 2 often denote the working solution and stock, respectively. Notably, both sides of the equation must always use the same units for concentration and volume.

From a stock of 50X TAE buffer, make 500mL of 1.0X TAE buffer as follows:

[tex]C_{1} V_{1} = C_{2} V_{2}[/tex]

(50X)V1=(1.0X)(500mL)

∴V1=10mL of 50X TAE Buffer

Calculating the volume of the water-based solvent is also necessary to finish the preparation. The remaining amount of water is needed to make the appropriate amount of working solution, which is 500mL.

[tex]V_{water}[/tex]=500mL−10mL=490mL

As a result, 490mL of water is used to dilute 10mL of 50X TAE Buffer.

To learn more about buffer solutions, refer:-

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