What are three methods for detecting radioactively labeled DNA in an experiment?

Answers

Answer 1

Answer:

Limitations. detect low abundance DNA binding proteins from lysates. test binding site mutations using many probe configurations with the same lysate. test binding affinity through DNA probe mutational analysis. non-radioactive EMSA possible using biotinylated or fluorescently labeled DNA probes.

Explanation:

Answer 2

Answer:

Proteins interact with DNA through electrostatic interactions (salt bridges), dipolar interactions (hydrogen bonding, H-bonds), entropic effects (hydrophobic interactions) and dispersion forces (base stacking). These forces contribute in varying degrees to proteins binding in a sequence-specific or non–sequence-specific manner.

Explanation:


Related Questions

what supports and protects the chest cavity?

Answers

Answer: The bones of the chest — namely the rib cage and spine — protect vital organs from injury, and also provide structural support for the body.

Explanation:

what is the percentage of thymine in cow ?

Answers

Answer:

Together, adenine and thymine compose 70% of the segment. This means that 30% of the section is composed of guanine-cytosine pairs. Since these two bases will be equal in quantity, 15% of the DNA section will be cytosine bases.

Explanation:

Answer:

28% thymine in cows

Since 28% adenine and 22% guanine and 22% cytosine  

In what step of transcription does RNA polymerase bind to
the promoter on DNA?

a-Initiation
OR
b -Elongation
OR
C Termination

Answers

answer should be A. initiation :)

Which is an environmental factor that would MOST LIKELY have an adverse impact on the stability of an ecosystem?

Answers

It is desserts and oceans because the water helps

The climate change would most likely exhibit an adverse influence on the stability of an ecosystem.

Climate change impacts on ecosystems:

Climate change influences ecosystems in numerous ways. Warming may make the species to migrate to higher elevations or latitudes where temperatures are more favorable.

Climate change is resulting in the elevation of sea level, resulting in intrusion of seawater into the freshwater system, which is forcing the key species to relocate. Thus, removing the prey or predators, which are essential for the stability of the ecosystem.

Due to climate change, the vegetative biomes in the United States is predicted to change across 5 to 20 percent by 2100. A specific species due to climate change ca ripple through a food web and influence a broad array of other species, thus, disrupting the food web.

Change in climate and shift in ecological conditions could support the spread of parasites, pathogens, and diseases with potentially adverse influences on agriculture, human health, and fisheries. Climate change, along with pollution and habitat destruction is one of the stressors, which can result in the extinction of species.

Thus, climate change is the environmental factor, which would most likely exhibit an adverse influence on the stability of the ecosystem.

Find out more information about the impact of climate change on the ecosystems here:

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How are signals “transmitted” into the cell?

Answers

Answer:

Generally, it is the brain that sends signals to parts of the body. It'[s the main headquarters for transmissions and orders.

Explanation:

Inside cells, electrical signs are passed on along the cell layer. Second, for correspondence between cells, the electrical signals by and large are changed over into compound signs passed on by little courier particles called synapses.

aha aha i need help please

Answers

Answer:

?explaining ????????????

how is the plant able to obtain each ingredient

Answers

Answer:

light enters a plant through its leaves O2 enters the plant through the stomata's or open pores

and water enters through the roots then up the stem to the tubes  to leave

Explanation:

hopefully this helps

The Plants able to obtain each ingredients from the atmosphere. the light from the sunlight, [tex]CO2[/tex] from the stomata, and the water from the roots.

Stomata allow plants to absorb [tex]CO2[/tex] from the atmosphere. Water is taken from the earth by plants and transmitted to their leaves via their roots. The only source of light that the chlorophyll and other green parts of the plant absorb is sunlight.

Plants use sunlight, water, and carbon dioxide to perform photosynthesis.

They produce Chlorophyll, the pigment that makes plants green, by using stored carbon, hydrogen from water, and magnesium from the soil. They make Adenosine triphosphate, which is vital in energy transfer, by combining phosphorus, oxygen, and carbon.

Thus, Plants take nutrient from the atmosphere . light from the sunlight, [tex]CO2[/tex]  from the stomata, and the water from the roots.  

Learn more about Plants here,

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A diagram showing two plates colliding, with one plate moving below the other. What type of plate boundary is illustrated in the image? What is the movement of one plate below another called?

Answers

Answer:

Hello There!! :D

Explanation:

Your answers are:

1. Convergent

2. Subduction

Hope this helps you!! ♥️♥️♥

- abakugosimp

Answer:

A. Convergent

B. Subduction

Explanation:

What type of plate boundary is illustrated in the image?

convergent

What is the movement of one plate below another called?

subduction

best answer gets brainliest

Answers

Answer: The person should steer around a breaking wave. This type of waves contains large amount of potential energy that is waiting to be transformed into Kinetic energy, In this case, it can impact the ship with tremendous force.

Explanation: Hope this help :)

PLEASE HELP QUICK!!!

How is earths surface most likely to change as the water freezes and expands on a mountain side over time?

Erosion will cause the ice to crack the rock

Physical weathering will cause the ice to crack the rock

Chemical weathering will cause the water to dissolve the rock

Disposition will cause the water to break the rock into sediment

Answers

The earths surface most likely to change as the water freezes and expands on a mountain side over time because Physical weathering will cause the ice to crack the rock.

What is soil erosion?

Erosion is nothing more than the displacement of land that happens from one place to another. In nature, the erosive process occurs by the natural action of the sun, winds and, mainly, rainwater.

It is a natural procedure, however, human action contributes to its intensification. The uncontrolled erosion process causes great damage to the environment, as it acts on the wear of the soil, makes it difficult to maintain species of animals and plants, in addition to disrupting human activities.

In this case, Erosion involves the wearing down, transport and deposition of soils and rock particles. So, the Physical weathering will cause the ice to crack the rock.

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the 2 kingdoms that include organisms that are decomposer

Answers

Explanation:

That would be Kingdom Fungi and Kingdom Monera.

Answer:

Fungi and monera

Explanation:

hope this helps :]

True or False...The Stereoscope/Dissecting Microscope is used to view small specimens.

Answers

i guess the statement is false

Do guys like tall girls or short girls? Is 5'5 tall or short?

Answers

Answer: 5'5 is average.

Explanation:

Over various spans of time, human populations have increased, decreased, or remained the same, and at both global and local levels. Which statement best explains this observation?

Answers

Answer:

C. Like other populations, the human population is affected by a variety of factors in the environment

Note: The options are found in the attachment below

Explanation:

Factors that affect population in the natural world include; availability of food, water, shelter, space, competition for resources, predation, as well as disease.

These factors also affect human population resulting in an increase, decrease or stability in population.

Regarding decrease in population, factors like competition for scarce resources has resulted in wars which greatly reduced human population. Also disease outbreaks, droughts, famines, etc have resulted in decrease in population.

Availability of food, water, shelter and space on the other hand makes for an increase in population by increasing the birth rate, reducing competition and bringing stability.

In some other instances, overtime, there is a balance between birth and death rate, so human population will remain relatively unchanged at these times.

*Please Help* Which of the following organisms would you expect to be most abundant in the tallgrass prairie ecosystem?

A. Grasses and flowering plants
B. White-tailed deer and cottontail rabbits
C. Bobcats and Coyotes
D. Harriers and kestrels

Answers

Answer:

A

Explanation:

Prairies are unique ecosystems dominated by grasses and other non-woody plants called forbes. ... A prairie ecosystem includes all the living components-- plants and animals--and all the nonliving components-- sun, water, fire, soil, and--nutrients.

The tallgrass prairie is a north American ecosystem and is famous for large grazing animals. The ecosystem consists of forests, grasslands, and savanna.

The tallgrass is capable of supporting significant species. Parts of the region have about top reptiles, birds butterflies, and trees.  Many of the species are found in the understory layer. This biome depends on the barriers files. Tallgrass is home to a diverse number of species such as cattle, bison, harriers, coyotes, and bobcats.

Hene the ecosystem of the tallgrass is dominated by the organisms like the Bobcats and Coyotes. This option C is correct.

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A model of a biological process is shown. What is the purpose of this process?

a) to assemble nucleotides in an mRNA chain along a DNA template
b) to replicate the DNA of an organism before cell division
c) to synthesize amino acids used to unzip strands of DNA and copy the genetic code
d) to translate the genetic code into a specific sequence of amino acids

Answers

Answer:

The correct answer is - option D.  to translate the genetic code into a specific sequence of amino acids

Explanation:

The given model in the figure is the process of translation during protein synthesis. The translation is the process that translates or converts the code carried from the nucleus by the process of transcription which is creating a complementary sequence of mRNA from DNA.

Translation completes in three basic stages initiation, elongation and termination. It is completed with ribosomes; a machine that builds peptide chain by binding mRNA bind to it, tRNA brings the correct amino acids to the ribosome to make peptide chain according to the mRNA code.

The purpose of the biological process shown in this model is: D. to translate the genetic code into a specific sequence of amino acids.

Gene expression in living organism is mainly controlled on two (2) main levels and these are:

Transcription.Translation.

Translation can be defined as a biological process through which a genetic code from a sequence of messenger RNA (mRNA) molecule is decoded (translated) to a specific sequence of amino acids during the synthesis of protein.

Form the model, we can deduce that genetic code from a sequence of messenger RNA (mRNA) molecule are being translated into a specific sequence of amino acids.

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PLEASE PLEASE ANSWER ASAP
How is photosynthesis and cellular respiration related?

Answers

Answer:

They go hand in hand as a cycle.

Photosynthesis converts carbon dioxide and water into oxygen and glucose. Glucose is used as food by the plant, and oxygen is a by-product. Cellular respiration converts that glucose and  oxygen into water and carbon dioxide, which then go through photosynthesis again.

Petrified wood is a beautiful material that forms over thousands of years. It occurs when forests get covered in rock and sediment. Which statement is true?


1. It is a mineral now that the Earth has changed it

2. It is not a mineral because the material was organic

3. It is a mineral as long as it displays the crystal structure

4. It is not a mineral because it has a definite composition

Answers

Answer:

4. It is not a mineral because it has a definite composition

I need a huge explanation!! I’ll give brainliest.

Answers

Answer:

Deep-ocean tsunami detection buoys are one of two types of instrument used by the Bureau of Meteorology (Bureau) to confirm the existence of tsunami waves generated by undersea earthquakes. These buoys observe and record changes in sea level out in the deep ocean.

Explanation:

hope this helps.. if correct give me brainliest plz!!!!

Dogs have 39 chromosomes. Each chromosome consists of a pair of identical chromatids attached together by a structure called a centromere. Once the chromosome has split, each chromatid is called a daughter chromosome. At the end of cytokinesis, how many daughter chromosomes will be found in each dog cell? 19 19.5 23 39 46

Answers

Answer:

39

Explanation:

Answer:

39

Explanation:

The cells copy themselves so there is one original and one new. We only count the new so it is still 39.

Use the weather map to answer the question.

Weather map with blue line with triangles pointing down and to the right. Red line with semi-circles pointing up and to the right. Red line is to the right of the blue line.
© Dorling Kindersley / Universal Images Group / Image Quest 2016

What does the red line indicate?

A cold front is moving to the north and east.
A cold front is moving to the south and west.
A warm front is moving to the north and east.
A warm front is moving to the south and west.

Answers

Answer:

It is C!

Explanation:

when they are half circles on the line, that means it will be a warm front.

The other answerer got it wrong

4Fe2O3

Fe =

O =


,mlhk

Answers

Answer:

Fe stands for iron and O stands for oxygen in the periodic table.

Explanation:

I don't really know what you need help with :/

What evolutionary advances are seen in the phyla of bilaterally symmetrical worms?

Answers

Bilaterally symmetrical worms are better adapted for actively hunting prey and performing complex behaviors. They have a rudimentary brain and a central nervous system that help them coordinate body movements, sense their surroundings, and pursue prey.

As human travel increases, the number of introduced species likely,
A) Decreases
B) Increases
C) Stay the same
D) Is eliminated

Answers

Answer:

B), increases

Explanation:

because as human travels the More they introduce new specis

During cellular respiration, the bonds of food molecules are broken, so energy can be released to fuel other cellular processes. In order for this to occur, which of the following statements must be true?

Answers

Chemical potential energy is stored in glucose's carbohydrate bonds, and these bonds can be broken to fuel the production of ATP. Energy in ATP can then be used to perform cellular work.

That should answer your question.

Answer:  New compounds with lower energy bonds must be formed when the high energy bonds in food molecules are broken.

Explanation: because it is right

is tested on a test is 100% right

where did the swine flu originate at in the Maryland County Fair?

Answers

Answer:

The 2009 swine H1N1 flu pandemic -- responsible for more than 17,000 deaths worldwide -- originated in pigs from a very small region in central Mexico, a research team is reporting

Explanation:

what would this represent?

a) multicellular, eukaryote, autotroph
b) unicellular, eukaryote, autotroph
c) multicellular, prokaryotic, heterotroph
d) unicellular, prokaryotic, autotroph

Answers

Answer:

A

Explanation:

How many different kinds of atoms does an element contain?

A) 2
B) 1
C) 8
D) 4

Answers

Answer:

an element contain 2 atoms

Answer: Your just a kid who doesn't know anything

i will give brainliest to whoever can answer this science riddle first and accurately :)
I’m at the beginning of earth’s timeline, but at the end of life’s start. I was there when a T-rex began, but in the middle of it’s asteroid end. What am I?

Answers

I believee it's the Cretaceous Period?

All of the dinosaur periods are at the start of Earth's timeline, and "the end of life's start" suggests the end of dinosaurs, meaning, when the asteroid hit. So it's probably one of the Mesozoic periods.

The T-rex first appeared in the Cretaceous Period, and the asteroid hit somewhat during middle of the Cretaceous Period.

I hope I'm right, and I hope this helps!

How are karyotypes utilized in diagnostic medicine?
1 page essay

Answers

Answer: they are used usually in the congotitus form of medicine

Explanation: connecus

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