the following figure shows the general steps that occur when a researcher uses the crispr-cas9 system to modify a protein-encoding gene in a eukaryotic cell with the goal of modifying the protein product. drag the descriptions of the steps to their appropriate locations on the figure.

Answers

Answer 1

A scientific advance has been made thanks to CRISPR-Cas9 editing. Many scientists are interested in this and have successfully edited DNA using this method.

Although the use of guide RNA is not new, it has made great strides in directing caspases toward their target sequences.Although the mutations have been reported, they cannot yet be verified. Although it has been reported that CRISPR-Cas causes single base pair deletions in DNA far from its activity site, which lead to gene impairment, the processes of DNA alterations through CRISPR-Cas have not yet established any form of reliable approach.After CRISPR was used to edit DNA, significant changes were seen in DNA sequences thousands of bases away from the region of action.

However, there is currently no experimental data that reveals the specific mechanism.

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Related Questions

What are Bioreactors?​

Answers

Answer: Bioreactor is an apparatus for growing organisms (yeast, bacteria, or animal cells) under controlled conditions.

Explanation:

There are 5 types of bioreactors.

(1) Continuous Stirred

(2) Bubble Column Bioreactors.

(3) Airlift Bioreactors.

(4) Fluidized Bed Bioreactors.

(5) Packed Bed Bioreactors.

A manmade device called a bioreactor is used to conduct biological reactions.

The bioreactor is a closed system that fosters the development of cells or tissues and is utilized in bioprocessing. Anaerobic or aerobic processes are both possible. Fermenters are often where bacterial reactions happen, whereas bioreactors are where cell growth happens.

Typically cylindrical in shape, bioreactors come in a variety of sizes from litres to cubic meters. They are typically constructed from stainless steel.

Pharmaceuticals like antibiotics and insulin are produced in bioreactors. They are sterile vessels equipped with the necessary temperature controls and gassing equipment to initiate a biological reaction.

The best conditions for optimum cell development and production will result from control over temperature, moisture, pH level, oxygen levels, and stirring rate.

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A dam is built that separates a river into two sections—a deep upstream section and a shallow downstream section. When the dam is first built, a fish species is able to move from the upstream section into the downstream section, but not vice versa. Predators can more easily catch the fish in the shallow downstream section than they can in the deep upstream section. Which scenario is most likely to lead to speciation?

A. The fish population contains individuals with effective predation evasive traits and eventual changes to the dam prevent fish from moving downstream.
B. The fish completely lack any effective predation evasive traits across the entire population, move infrequently from upstream to downstream locations, and reproduce with low frequency.
C. The fish reproduce with high frequency, move with high frequency from upstream to downstream locations, and have a robust set of predation evasive traits across the entire population.
D. The rate of upstream fish entering the downstream section decreases over time without being completely shut off, and many of these fish carry predation evasive traits.

Answers

Scenario A is most likely to lead to Speciation

Speciation is the development of an entirely new species from a given population over the course of evolution.

A group within a species undergoes speciation when it diverges from other individuals in the species and acquires its own distinct traits. The new species will be distinct from its ancestors due to environmental requirements or individual traits of the members of the new group.

Speciation requires:

.Geographical barrier.

.Natural selection.

.Genetic drift.

.Large mutation.

Here scenario is the only case where all conditions are met as eventual changes to the same provide a Geographical barrier.

Certain individuals have predator evasive traits which favor natural selection

The movement of fishes from Upstream stream to Down the stream is genetic drift.

So in the situation A the speciation will most likely to occur because of the invasive of the predators.

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3) Fill out the graphic organizer below based on the reading above.
Connective Tissues

Answers

Connective tissues consist of a ground substance, a non-living substance, and a matrix of living cells.

Tissue That Contains Fibers

In fibrous connective tissues, which have few cells or other forms of matrix, collagen fibers predominate. The fibers' strands can be arranged either randomly or in parallel. Fibrous connective tissues are distributed unevenly in areas of the body that are constantly under stress, such as the dermis of the skin

Cartilage

Cartilage is a type of connective tissue that has a substantial amount of matrix and various numbers of fibers. The chondrocyte cells produce the tissue's matrix and fibers. Lacunae, which are voids in the tissue, are where chondrocytes are found.

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i need help with this please!

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The plant should completely stop manufacturing high-energy sugars, as expected. Due to the fact that photosynthesis requires CO2, a plant without CO2 will not be able to produce energy and would most likely perish.

What is meant by photosynthesis ?Plants and other living things employ a process called photosynthesis to transform light energy into chemical energy that may then be released through cellular respiration to power the organism's activities. The word "photosynthesis" comes from the Greek words "light" and "putting together," and refers to the process of creating molecules of carbohydrates from carbon dioxide and water. These molecules, such as sugars and starches, are then stored with some of this chemical energy. Photoautotrophs are creatures that perform photosynthesis, including most plants, algae, and cyanobacteria. The majority of the energy required for life on Earth is produced and maintained through photosynthesis, which is also substantially responsible for creating and maintaining the oxygen concentration of the atmosphere.

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Label the parts of the neuron cell

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Provided that neurons are diverse in their roles as well as their shapes and sizes, not all of them have parts of the exact same size. Generally, a neuron can be broken down into its four main components: the soma, dendrites, axon, and axon terminals.

The soma of the neuron is essentially the neuron’s body. Due to the nucleus’ being located within it, the majority of protein production gets done right here.

As you may have seen in other answers, a neuron also has input points, which are known as dendrites. In the diagram, they are the protruding branch filaments on the left hand side of the neuron. To be simple, the dendrites are responsible for transmitting electrical signals, from the axon of other neurons, to the soma. This electrical transmission is done through points known as synapses found in the axon terminal of a neighboring neuron.

To elaborate more on the axon, it is the extensive fiber that carries nerve signals emitted by the soma. The signals first enter the axon from the soma at a point which is referred to as the axon hill, where the action potential is generated, or simply a nerve impulse that travels across and affects the structural polarity of the neuron.

They will then be transmitted to the neuron’s very own axon terminal, the branches on the right hand of the diagram, which are interconnected in a network with more neurons at their dendrites.

A neutron in its rest state is negatively charged, as the axon interior is roughly 70 mV more negative than the exterior.

So, to briefly summarize and put things in order: dendrites, the protruding branches on the left hand of the diagram, will receive the electrical signals from the synapses of a nearby neuron. These electrical signals will be transmitted to the soma, the “body” of the neuron, responsible for creating proteins, which contains the nucleus (the ellipse in the diagram). From here, these signals will be transmitted across the axon, the long filament seen above, as a nerve impulse that affects the polarities of the membrane as it travels through. It will then be sent to the axon terminal at the end of the cell, which forms a gap with the dendrites of neighboring neurons. This gap, as aforementioned, is known as the synapse.

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Preganglionic sympathetic fibers exit the spinal cord via the __________ root and pass through the __________ to enter the __________.

Answers

Rami communicate and flow through the sympathetic trunk to enter the sympathetic trunk ganglia as preganglionic sympathetic fibres leave the spinal cord through the ventral root.

Preganglionic fibres in the autonomic nervous system are those that run from the central nervous system to the ganglion. No matter if they are in the sympathetic or parasympathetic division, preganglionic fibres are all cholinergic (that is, they use acetylcholine as their neurotransmitter) and myelinated. Due to sympathetic ganglia frequently being closer to the spinal cord than parasympathetic ganglia, sympathetic preganglionic fibres typically have a shorter length than parasympathetic preganglionic fibres. Divergence is one of the two ANSs' (autonomic nervous systems') most significant differences. On the other hand, the parasympathetic division has a ganglia.

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Many people believe that all micro-organisms are harmful. Give two examples of how unicellular organisms, such as bacteria and fungi, are useful to humans and the environment.

Answers

1) Fungi is a decomposer, meaning it breaks down dead organisms and repurposes it. With out Fungi, a common decomposer, the whole ecosystem would perish.

2) Not all bacteria is bad, most of the bacteria in our bodies is what protects us from harmful things. In fact, humans carry more bacterial cells

a large decrease in resistance is seen in the blood vessels supplying working skeletal muscle during exercise. what is the effect of this?

Answers

The effect of this Dilation of arterioles.

Skeletal muscles contain 30 to 40% of your total frame mass. they may be the muscle tissue that connects to your bones and allow you to perform a huge variety of moves and capabilities. Skeletal muscular tissues are voluntary, meaning you manage how and once they work.

Skeletal muscle groups permit human beings to transport and perform daily sports. They play an important function in breathing mechanics and assist keep posture and balance. in addition, they guard the crucial organs within the frame.

Skeletal muscle, also known as voluntary muscle, in vertebrates, maximum not unusual of the 3 sorts of muscle in the frame. Skeletal muscle tissues are attached to bones with the aid of tendons, and they produce all of the moves of body parts in relation to every other.

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the timing of a mutation during development has negligible effects on the severity of the genetic defect. State True or False your answer:
a. True
b. False

Answers

False. On the severity of the genetic abnormality, the timing of a mutation during development has very little of an impact.

Genes serve as an example.

The genes you inherit influence a variety of traits, including your skin and hair hues. Emma's mother could have two genes—one for brown hair and one for red hair—and may have passed one of them on to her. If her father has two copies of the gene that causes red hair, she could get it.

Genes' relevance

The instructions contained by your genes control the development and operation of your cells. Cells are the building blocks of the body. Every part of your body is made up of a billion cells working together. The components that keep genes in place called chromosomes.

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why does a lack of proton gradient being produced, the atp synthesis would cease which causes metabolic problem

Answers

Without a proton gradient, protons will not flow through ATP synthase, hence no ATP will be produced.

What happens if ATP synthesis is blocked ?

The output of ATP from OXPHOS is compromised by ATP synthase inhibition, and the energy metabolism is reprogrammed to favour enhanced glycolysis.

The proton motive force drives the synthesis of ATP once there are enough protons present. Therefore, a gradient enables cells to accumulate protons as "loose change," and this difference is crucial in determining whether there is growth or not, and whether there is life or not.

Protons need more and more energy to be pushed across the gradient as it increases. When the energy needed to push protons reaches 69.5 kJ/mole, electron transport must stop. In fact, before the gradient reaches that level, the second law of thermodynamics requires that electron transport cease.

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It is not likely that any living organism could survive on the Martian surface because of
O ultraviolet radiation
O warm temperatures
O abundance of water

Answers

It is not likely that any living organism could survive on the Martian surface because of Ultraviolet radiation. Thus, the correct option for this question is A.

What is the Martian surface?

The Martian surface may be defined as a type of surface that is rocky in nature with canyons, volcanoes, dry lake beds, and craters all over it. Red dust covers most of its surface. It is basically made up of iron, nickel, and sulfur.

There have been organisms found in places on the Earth where everyone said it would be impossible to for anything to survive. This is because of the emission of high amounts of ultraviolet radiation that makes an environment unfit for an organism's survival.

Therefore, the correct option for this question is A.

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if an animal cell does not produce its own food how does it make energy to carry out the functions​

Answers

Answer:

by consuming other organisms to aquire the energy that needed

Genome editing has many advantages for treating and preventing diseases, however there are many ethical concerns. Explain one ethical concern related to genome editing.

Answers

One ethical concern related to genome editing is the possibility of editing the germinal line to obtain an embryo having desired physical and or cognitive features.

What are the ethical concerns of genome editing methods?

The ethical concerns of genome editing methods such as the CRISPR-Cas9 system are mainly based on the possibility to edit embryos or germinal cells to produce humans.

Therefore, with this data, we can see that the ethical concerns of genome editing methods are mainly based on the issues associated with the edition of the germinal line.

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Was it harmful when the Once-ler cut down the first tree? If so, who, or what did it harm?

Answers

Answer:

In general, cutting down trees can have negative effects on the environment and the creatures that live in it. Trees provide habitat and food for many different species of animals, and they also help to regulate the climate and prevent soil erosion. Cutting down trees can destroy habitat, disrupt the food chain, and contribute to climate change. Additionally, deforestation can have negative impacts on local communities and economies, particularly if the trees are a crucial source of livelihood for those who live in the area.Explanation:

a. How do climax communities relate to biomes?
b. What factors affect the characteristics of climax communities?
c. How has cattle grazing affected the ecological balance between the sage brush
and grasses in the American Midwest?

Answers

Answer:

A. Climax communities are the end point of the process of ecological succession, which is the gradual process by which a community of organisms changes over time in response to environmental changes or disturbances. Climax communities are characterized by the presence of species that are well adapted to the particular environmental conditions of the area, and they typically exhibit a high level of stability and self-regulation. Climax communities can be found within a variety of biomes, including grasslands, forests, and aquatic systems.

B. The characteristics of climax communities are influenced by a variety of factors, including climate, soil type, topography, and the presence or absence of certain species. For example, a climax community in a tropical rainforest is likely to be quite different from a climax community in a desert, due to the differences in temperature, humidity, and other environmental factors.

C. Cattle grazing can have a significant impact on the ecological balance between sagebrush and grasses in the American Midwest. Grazing can reduce the cover of sagebrush, which can create an opening for grasses to establish and dominate the plant community. This can result in changes to the ecosystem, including alterations in the habitat of animal species that depend on sagebrush for food or shelter. In addition, overgrazing can lead to soil erosion and other forms of environmental degradation. Proper management of grazing can help to minimize these negative impacts and maintain the ecological balance between sagebrush and grasses.

Explanation:

How could scientists today use current technologies to
further support the cell theory?

Answers

Answer:

There are several ways that scientists can use current technologies to further support the cell theory, which is the fundamental concept in biology that all living organisms are composed of cells. Here are a few examples:

1.Microscopy: Scientists can use advanced microscopy techniques, such as electron microscopy, to visualize cells at high resolution and study their structure and function in detail.

2.Molecular biology techniques: Scientists can use techniques such as DNA sequencing, gene expression analysis, and proteomics to study the molecular makeup of cells and understand how they function at the molecular level.

3.Cell culture: Scientists can use cell culture techniques to grow and study cells in the laboratory, allowing them to study the behavior of cells under different conditions and understand how they respond to various stimuli.

4.Animal models: Scientists can use animal models, such as mice or zebrafish, to study the biology of cells in a whole organism and understand how cells function in the context of a living organism.

Overall, these technologies allow scientists to study cells in great detail, providing further evidence and support for the cell theory.

which three statements may correctly explain why the population size increases after time point c?

Answers

The few germs that were drug-resistant swiftly multiplied, creating a large population of these microorganisms. Drug-resistant bacteria multiplied more rapidly between time points C and D than non-resistant

What do microorganisms do?

A few bacteria aid in food digestion, assist the body absorb vitamins, and eliminate disease-causing cells. Additionally, yogurt and cheese are produced using bacteria. However, you can get sick from contagious microorganisms. In your body, they multiply swiftly.

How did bacteria get started?

Bacteria are present in soil, groundwater, plants, wildlife, radioactive waste, the earth's crust itself, polar ice and glaciers, hot springs, and nuclear waste. Both the stratosphere, which is between 6 - 30 miles above the earth, and the ocean's depths, which reach 32,800 feet (10,000 meters), are home to bacteria.

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Which of the following is not a piece of evidence to support that climate is changing?
1. Satellite images showing smaller land cover of ice caps and glaciers
2. Buoys and floats measuring a decrease in sea surface temperature of ocean
3.Tidal gauges and satellites measuring the sea level has increased
4. Satellites and weather balloons showing temperature of lower atmosphere increasing.

Answers

Answer:

2

Explanation:

1,3, and 4 all show the climate is changing and the globe is warming.


3) Fill out the graphic organizer below based on the reading above.
Connective Tissues

Answers

Connective tissues consist of a ground substance, a non-living substance, and a matrix of living cells.

Tissue That Contains Fibers

In fibrous connective tissues, which have few cells or other forms of matrix, collagen fibers predominate. The fibers' strands can be arranged either randomly or in parallel. Fibrous connective tissues are distributed unevenly in areas of the body that are constantly under stress, such as the dermis of the skin.

Cartilage

Cartilage is a type of connective tissue that has a substantial amount of matrix and various numbers of fibers. The chondrocyte cells produce the tissue's matrix and fibers. Lacunae, which are voids in the tissue, are where chondrocytes are found.

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many of our immune cells kill target cells via apoptosis. what is not one of the advantages of killing via apoptosis?

Answers

To kill extracellular pathogens more efficiently  is not one of the advantages of killing via apoptosis

In multicellular organisms, apoptosis is a type of planned cell death that takes place. Characteristic cell changes (morphology) and death are caused by biochemical processes. Blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, DNA fragmentation, and mRNA degradation are some of these alterations. Apoptosis causes the average adult human to lose 50–70 billion cells daily. Around 20–30 billion human cells each day perish in an average human youngster between the ages of 8 and 14 years.

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Terrestrial plants have a waxy covering produced by the dermal tissues called the cuticle. If this waxy covering was overproduced so that the stomata became clogged, what would happen to the plant?.

Answers

The plant's capacity to carry out photosynthesis would've been constrained since it would be unable to exchange oxygen with carbon dioxide.

The plant will not be able to exchange Oxygen for Carbon dioxide as well as the plant's ability to carry out the process of photosynthesis would also be limited, if the stomata get clogged due to overproduction of cuticle.

All apical portions of land plants have waxy cuticles to reduce water loss. Their major job is to make it possible for gases like oxygen, water vapor, including carbon dioxide to enter and exit the leaf quickly.

The question is incomplete. The complete question is:-

Terrestrial plants contain a waxy covering produced by the dermal tissues known as the cuticle. Suppose this waxy covering was overproduced in such a way that the stomata became clogged, then what would happen to the plant?

(A) The plant will not be able to exchange Oxygen for Carbon dioxide as well as the plant's ability to carry out the process of photosynthesis would also be limited.

(B) The sunlight would not be able to penetrate the plant's ground tissue cells through the thick dermal tissue and the plant's ability to carry out photosynthesis would be harmed.

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Organisms that are closely related, but not direct members of the group being studied.

Answers

Paraphyletic is the answer.

23. Infant monkeys raised with a nourishing wire mother and a nonnourishing cloth mother:a. shifted their initial preference for the wire mother to the cloth mother as they matured.b. preferred the nourishing wire mother.c. preferred the nonnourishing cloth mother.d. showed no preference for one mother over the other.

Answers

Infant monkeys raised with a nourishing wire mother and a non-nourishing cloth mother (c) preferred the non-nourishing cloth mother.

Monkeys belong to the primate order of the class Mammalia. They are considered to be highly social as animals. They are usually present in groups which is known as troop. They are usually herbivores. The smallest monkey in the world is  Pygmy Marmoset monkey.

Cloth mother is the one who provides comfort and warmth to the child but us unable to provide nutrition.  This phenomenon is mostly observes in monkeys where the mother that allows the child to cling on to her is called the cloth mother.

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activation of what group of proteins in the plasma leads to inflammation, attraction of phagocytes, and enhancement of phagocytosis? multiple choice interferon lysosomes complement

Answers

Complement proteins. To opsonize pathogens and trigger a cascade of inflammatory reactions that aid immune cells in battling infection and preserving homeostasis, complement proteins work together.

How can phagocytic cells safeguard the body against encroaching pathogens?

Neutrophils are a subset of white blood cells called phagocytes. To prevent infection, these cells ingest bacteria and other pathogens. A phagocyte is drawn to a pathogen or damaged tissue by its chemicals, which trigger the process. After attaching to the microorganism and encasing it, the phagocyte consumes it.

What types of complement proteins are there?

C1, C2, C4, C5b, C5a, C3a, C6, C7, C8, C9, Factor B, Factor D, mannose-binding lectin, and properdin are a few examples of complement proteins.

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Which of these statements best explains how adaptation provides evidence to the scientific theory of evolution? (4 points) It proves that natural selection is the only means of evolution. It shows that organisms choose to adapt to changing Earth conditions. It proves that natural selection leads to greater complexity in organisms. It shows that some features in an organism help it survive better on Earth.

Answers

The correct statement that best explains how adaptation provides evidence to the scientific theory of evolution is It proves that natural selection leads to greater complexity in organisms.

What is evolution?

Evolution is described as change in the heritable characteristics of biological populations over successive generations.

In evolution, the survival of the fittest due to adaptation has resulted in increasing the variations and complexity of the organism which is supported by the evidence collected by Charles Darwin where he noticed that Galapagos finches showed the  variations in the shape of the beaks due to different niches.

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what are two features that all cells, in all three domains of life, have in common?

Answers

The genetic material, the bilipid layer of the plasma membrane, and the cell cytoplasm are the three characteristics of cells that are most frequently seen in all three cell domains.

What features do all three spheres of life share?

The domains of life show how cellular transformation has evolved. Each of them is related to a single Last Universal Common Ancestor (LUCA). The main similarities between archaea and bacteria are their small size, lack of nuclei, and preference for RNA over DNA.

Do all three of the biological domains have a nucleus?

Bacteria, Archaea, and Eukarya are the three domains that make up all life on earth. The first two are made entirely of microorganisms with one cell. They are all without a nucleus. Eukarya, the third domain, is made up of organisms whose cells have a nucleus.

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the idea that life and earth are symbiotic is called the . a. dna hypothesis b. proton conjecture c. gaia hypothesis d. neutrino hypothesis

Answers

The idea that life and Earth are symbiotic is called the Gaia hypothesis.

The Gaia hypothesis definition, which can be stated as the interaction between living things on Earth and their inorganic surroundings creating a complex, self-regulating, and synergistic system that helps maintain ideal conditions for life on the planet, can help people understand what the term means. It was proposed that the Gaia principle may be used to find signs of life in other planets' atmospheres. James Lovelock's Gaia idea was a reasonably affordable and trustworthy technique to employ such interactive combinations to look for the possibility of life on worlds other than Earth.

Hence, life entirely dependent on earth resources.

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a plant cell is placed in a hypotonic solution. what will happen to the plant cell?

Answers

The cells take up water from the external medium and swell up.

Plant cells are the cells found in green flora, photosynthetic eukaryotes of the kingdom Plantae.

The elements of a plant cell and plant cell components, so one can be mentioned, are plant mobile wall, plant mobile membrane, smooth endoplasmic reticulum, ribosomes, hard endoplasmic reticulum, vacuole, nucleus, peroxisomes, Golgi apparatus, mitochondria, cytoplasm, cytoskeleton, and plastids.

Plant cells are the building blocks of plants. Photosynthesis is the predominant characteristic performed with the aid of plant cells. Photosynthesis occurs inside the chloroplasts of the plant mobile. it's miles the system of getting ready meals by using the plants, through making use of sunlight, carbon dioxide, and water.

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How did scientists boost the spacecraft Juno on its way to Jupiter, helping it overcome the gravitational pull of the Sun?
A.
by using two rockets
B.
by using a gravitational slingshot
C.
by decreasing the mass of Juno
D.
by increasing the fuel on the rocket

Answers

Answer is B: by using a gravitational slingshot

the major anatomic difference in autonomic reflex arcs is that there are two sensory afferents.

Answers

There are two motor neurons, called pre- and postganglionic motor neurons, in autonomic reflex arc pathways, which is the main anatomical distinction.

What two divisions make up the autonomic nervous system?

Two major divisions make up the autonomic nerve system: Sympathetic. Parasympathetic.

Which two cranial nerves contribute to this autonomic reflex?

Different receptors in the heart, lungs, and vasculature send sensory information to the brainstem via the glossopharyngeal and vagus cranial nerves. This afferent information is essential for the immediate control of arterial blood pressure as well as the dilating of mental and physical stimuli. In times of stress or danger, this system triggers bodily functions that can assist you.

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