The 10x SDS gel electrophoresis buffer contains 250mM Tris HCl, 1.92M Glycine, and 1% (w/v) SDS. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so you will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and SDS in the 1x buffer

Answers

Answer 1

Answer:

25 mM Tris HCl and 0.1% w/v SDS

Explanation:

A 10X solution is ten times more concentrated than a 1X solution. The stock solution is generally more concentrated (10X) and for its use, a dilution is required. Thus, to prepare a buffer 1X from a 10X buffer, you have to perform a dilution in a factor of 10 (1 volume of 10X solution is taken and mixed with 9 volumes of water). In consequence, all the concentrations of the components are diluted 10 times. To calculate the final concentration of each component in the 1X solution, we simply divide the concentration into 10:

(250 mM Tris HCl)/10 = 25 mM Tris HCl

(1.92 M glycine)/10 = 0.192 M glycine

(1% w/v SDS)/10 = 0.1% w/v SDS

Therefore the final concentrations of Tris and SDS are 25 mM and 0.1% w/v, respectively.


Related Questions

Set Grouper to 70%. How do you think this level of fishing will affect the populations of the other fish in the simulated reef? Explain your reasoning.

Answers

Answer:

Greatly affect.

Explanation:

This level of fishing will greatly affect the populations of the other fish in the simulated reef because overfishing disturbs the equilibrium in the marine ecosystem. One fish is a food of another fish or the big fish act as a controller which controls the population of other small fishes and in that way the ecosystem is present in equilibrium state so overfishing will greatly affect the marine ecosystem.

The diagram below represents the organizational levels of living things.
organisms
organ systems
?
tissues
cells
Which of the following examples represents the missing organizational level?
A blood
B heart
C plasma
D vein

Answers

Answer: its vein

Explanation:

Answer: It’s blood

Explanation:

I did the quiz

In a chemical change
A. materials retain their properties.
B. materials change their properties.
C. new materials do not form.
D. new materials formed are always in liquid form.

Answers

Answer:

B. materials change their properties.

Explanation:

In a chemical change, materials often change their properties because a re-arrangement of atoms takes place.

A chemical change is one in which new kind of matter is formed.

It is always accompanied by energy changes.

Chemical changes are not reversible. They lead to the production of new kinds of matter It involves mass changesRequires considerable amount of energy

6. 7. A hyperbaric chamber has a volume of 200. L. (a) How many moles of oxygen are needed to fill the chamber at room temperature (23°C) and 3.00 atm pressure? b) How many grams of oxygen are needed? (Hint: Since moles have been asked, which equation has the moles listed in the equation. Use that to solve this problem. Also don’t forget to use the equation 1 mole = Formula weight or Molecular weight to calculate the grams of O2).

Answers

Answer:

a) 24.7 mol

b) 790 g

Explanation:

Step 1: Given data

Volume of the chamber (V): 200. LRoom temperature (T): 23 °CPressure of the gas (P): 3.00 atm

Step 2: Convert "T" to Kelvin

We will use the following expression.

K = °C + 273.15

K = 23°C + 273.15 = 296 K

Step 3: Calculate the moles (n) of oxygen

We will use the ideal gas equation.

P × V = n × R × T

n = P × V/R × T

n = 3.00 atm × 200. L/(0.0821 atm.L/mol.K) × 296 K = 24.7 mol

Step 4: Calculate the mass (m) corresponding to 24.7 moles of oxygen

The molar mass (M) of oxygen ga sis 32.00 g/mol. We will calculate the mass of oxygen using the following expression.

m = n × M

m = 24.7 mol × 32.00 g/mol = 790 g

Which is a nonmetal that becomes isoelectronic with argon
when it forms its most common ion?

Answers

Answer:sulfur

Explanation:

Dwarf planets are smaller than planets. What is another way that dwarf planets are different?

Answers

Answer:

dwarf planets lack the gravitational forces needed to pull in and accumulate all of the material found in their orbits

Explanation:

HELP IT’S ABOUT TO BE DUE!

Answers

The answer is b
Explanation:

Jupiter has about 317 times more mass than Earth. What can you infer from this
fact?

Answers

Answer:

That Jupiter's gravitational force is much stronger than Earth's gravitational force.

A sample of a certain organic acid has a mass of 5.000 g. Elemental analysis of this compound found it to contain 1.334 g C, 3.554 g O, and the rest of the mass is hydrogen. If the molar mass of the organic acid is 90.03 g/mol, determine its molecular formula.

Answers

Answer:

C₂H₂O₄

Explanation:

Given parameters:

Mass of organic acid  = 5g

Mass of C  = 1.334g

Mass of O = 3.554g

Mass of H = 5  - (1.334 + 3.554)  = 0.112g

Molar mass  = 90.03g/mol

Unknown:

Molecular formula of the compound  = ?

Solution:

To solve this problem, we find the empirical formula of the compound first. It is the simplest formula of the compound.

Elements                        C                     H                        O

Mass                             1.334               0.112                   3.554

Molar mass                       12                   1                          16

Number of moles          1.334/12          0.112/1                 3.554/16

                                         0.112              0.112                     0.222

Divide by the

Smallest                         0.112/0.112        0.112/0.112          0.222/0.112

                                                1                       1                      2

           Empirical formula  = CHO₂  

The molar mass of this empirical formula  = 12 + 1 + 2(16) = 45g/mol

Now;

  Multiplication index  = [tex]\frac{90.03}{45}[/tex]    = 2

Molecular formula  =  (CHO₂)₂    = C₂H₂O₄  

Every morning, Jeremiah uses a blender to make a smoothie for breakfast. Which of the
following shows the energy transformation that the blender demonstrates?
А
Electrical - light
B
Chemical → mechanical
с
Mechanical → chemical
D
Electrical mechanical

Answers

Answer:

D

Explanation:

Answer:

D: Electrical -> mechanical

Explanation:

Hope this helps!

Name the alkyne. Spelling and punctuation count. A seven carbon chain with a triple bond between carbon 1 and carbon 2. There is a C H 2 C H 2 C H 3 group attached to carbon 3.

Answers

Answer: IUPAC name of [tex]CH\equiv C-CH_2-CH_2-CH_2-CH_2-CH_3[/tex] will be hept-1-yne.

Explanation:

The IUPAC nomenclature of organic compounds are given as follows:

1. Select the longest possible carbon chain.

2. For the number of carbon atom, we add prefix as 'meth' for 1, 'eth' for 2, 'prop' for 3, 'but' for 4, 'pent' for 5, 'hex' for 6, 'hept' for 7, 'oct' for 8, 'nona' for 9 and 'deca' for 10.

3. A suffix 'yne' is added at the end of the name if triple bond is present.

[tex]CH\equiv C-CH_2-CH_2-CH_2-CH_2-CH_3[/tex]

The IUPAC name will be hept-1-yne.

identify the equipment best suited for extinguishing each type of fire. Your choices are: fire extinguisher, overturned beaker, fire blanket.

Answers

Answer: The equipment best suited for extinguishing each type of fire are listed below:

--> Fire extinguisher: Large fire on the floor

--> Overturned beaker: Small benchtop fire

--> Fire blanket: clothing fire.

Explanation:

Fire outbreaks is a common event that can be prevented in a standard chemistry laboratory with the aid of fire safety equipments.

The method used by these equipments to extinguish fire is to suffocate the fire by obstructing the source of oxygen, by reducing the heat with the use of water and removing the fuel or oxygen source.

These fire safety equipments should be highly visible and easily accessible in a laboratory. Example of fire safety equipments includes:

--> Fire extinguisher: A laboratory personnel needs to be trained in the use of this since it's made up of different types. it can be used to extinguish ( put out) large fire on the floor.

--> Overturned beaker: this can be used to suffocate small fire on the bench which is also common in the laboratory.

-->Fire blanket: This should be used in the event that a person’s body or clothing catches fire.

9. Calculate the specific heat capacity of titanium if a 43.56 g sample absorbs 0.476 kJ as its temperature changes from 20.5 oC to 41.2 oC.

Answers

Answer:

c = 0.528 J/g.°C

Explanation:

Given data:

Mass of titanium = 43.56 g

Heat absorbed = 0.476 KJ  = 476 j

Initial temperature = 20.5°C

Final temperature = 41.2°C

Specific heat capacity = ?

Solution:

Specific heat capacity:

It is the amount of heat required to raise the temperature of one gram of substance by one degree.

Formula:

Q = m.c. ΔT

Q = amount of heat absorbed or released

m = mass of given substance

c = specific heat capacity of substance

ΔT = change in temperature

ΔT = 41.2°C - 20.5°C

ΔT = 20.7 °C

476 J = 43.56 g × c × 20.7 °C

476 J = 901.692 g.°C × c

c = 476 J / 901.692 g.°C

c = 0.528 J/g.°C  

what happens to the electrons in a covalent bond?

Answers

Covalent bonding occurs when pairs of electrons are shared by atoms, Atoms will covalently bond with other atoms to gain more stability.

Answer:

Covalent bonding occurs when pairs of electrons are shared by atoms. Atoms will covalently bond with other atoms in order to gain more stability, which is gained by forming a full electron shell. By sharing their outer most (valence) electrons, atoms can fill up their outer electron shell and gain stability.

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Gravity pulls rain and snow down to Earth from the atmosphere through a paire
process called precipitation Water is pulled from elevated areas such as
mountains and hills into lakes, oceans, and water reserviors. What is this
describing?*
role of gravity in the water cycle
role of gravity in condensation
O
role of gravity in evaporation
role of gravity in precipitation

Answers

role of gravity in condensation.

You want to compare the malleability of
two metals. Plan an investigation that would allow you to determine
which metal is more malleable .

Answers

One way you could measure this is hitting the two metals with a mallet and seeing which one has more of a dent

The temperature and the strength of the metallic link are the two most crucial variables that can impact how malleable a metal or alloy is.

What is metal ?

A metal is a substance that has a shiny look when freshly processed, polished, or shattered, and conducts electricity and heat rather effectively. Generally speaking, metals are malleable and ductile.

The amount of pressure that a metal can sustain without breaking can be used to gauge its malleability. Varied metals have different degrees of malleability because of variations in their crystal structures.

The temperature of the metal and the strength of the metallic connection are the two parameters that define how malleable a metal or metal alloy will be.

Thus,  The kind of the metallic connection can have a significant impact on how easily metal atoms can rearrange themselves.

To learn more about metal, follow the link;

https://brainly.com/question/18153051

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Answers

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How many formula units of CaO are in 32.7 g of CaO?

Answers

Answer:

3.51× 10²³ formula units

Explanation:

Given data:

Mass of CaO = 32.7 g

Number of formula units = ?

Solution:

First of all we will calculate the number of moles.

Number of moles = mass/molar mass

Number of moles = 32.7 g/ 56.1 g/mol

Number of moles = 0.583 mol

Number of formula units:

1 mole = 6.022 × 10²³ formula units

0.583 mol ×  6.022 × 10²³ formula units / 1 mol

3.51× 10²³ formula units

The number 6.022 × 10²³ is called Avogadro number.

Calculate the percent composition (percent by mass of each element) of NH4Cl.

Round to the nearest ONES place ((example: 12.34% = 12%))

Answers

Answer:

[tex]\%N=26.2\%\\\\\%H=7.5\%\\\\\%Cl=66.3\%[/tex]

Explanation:

Hello!

In this case, since the calculation of the percent composition of an element in a chemical compound is computing considering its atomic mass, subscript in the formula and molecular mass of the compound it is; for nitrogen, hydrogen and chlorine we have that ammonium chloride has a molar mass of 53.49 g/mol so the percent compositions are:

[tex]\%N=\frac{14.01*1}{53.49}*100\% =26.2\%\\\\\%H=\frac{1.01*4}{53.49}*100\% =7.5\%\\\\\%Cl=\frac{35.45*1}{53.49}*100\% =66.3\%[/tex]

Best regards!

An element has five isotopes. Calculate the atomic mass of this element using the information below. Show all your work. Using the periodic table, identify the element this is likely to be and explain your choice. (18 pts)
A) Isotope 1 – mass: 64 amu; percent abundance: 48.89%
B) Isotope 2 – mass: 66 amu; percent abundance: 27.81%
C) Isotope 3 – mass: 67 amu; percent abundance: 4.11%
D) Isotope 4 – mass: 68 amu; percent abundance: 18.57%
E) Isotope 5 – mass: 70 amu; percent abundance: 0.62%

Answers

Answer: Sol:-

Data provided in the question is :-

Atomic mass of isotope -1 = 64 amu

Atomic mass of isotope -2 = 66 amu

Atomic mass of isotope -3 = 67 amu

Atomic mass of isotope -4 = 68 amu

Atomic mass of isotope - 5 = 70 amu

Percentage abundace of isotope - 1 = 48.89 %

Percentage abundance of isotope -2 = 27.81 %

Percentage abundance of isotope - 3 = 4.11%

Percentage abundance of isotope-4 = 18.57%

Percentage abundance of isotope - 5 = 0.62 %

Formula used :-

Average atomic mass of an element =[ {(atomic mass of isotope-1 * percentage abundance of isotope-1) + ( atomic mass of isotope-2 * percentage abundance of isotope -2) + ( atomic mass of isotope -3 * percantege abundance of isotope-3 ) + ( atomic mass of isotope-4 * percentage abundance of isotope-4) + (atomic mass of isotope-5 * percentage abundance of isotope-5)} / 100]

Calculation :-

Put all the value in the formula :-

Average atomic mass of an element = [{(64 * 48.89) + (66 * 27.81) + (67 * 4.11) + (68 * 18.57) + (70 * 0.62)} / 100] amu

= [{(3128.96) + (1835.46) +(257.37) + (1262.76) + (43.4)} / 100] amu

= {(6528.04) / 100} amu

= 65.2804 amu

Average atomic mass of an element is = 65.2804 amu

Then this mass is approximatly equal to atomic mass of zinc so this element would be zinc

atomic mass of zinc = 65.38 \approx 65.2804 amu

A tire contains 1.50 mol of air at a gauge pressure of 205 kPa. If the volume of the air in the tire is 0.0120 m3, what is its temperature

Answers

Answer:

197.07 K

Explanation:

To solve this problem we can use the PV=nRT equation, where:

P = 205 kPa ⇒ 205 * 0.00986 = 2.02 atmV = 0.0120 m³ ⇒ 0.0120 * 1000 = 12 Ln = 1.50 molR = 0.082 atm·L·mol⁻¹·K⁻¹ T = ?

We input the data:

2.02 atm * 12 L = 1.50 mol * 0.082 atm·L·mol⁻¹·K⁻¹ * T

And solve for T:

T = 197.07 K

For the reaction of ammonia (NH3) with oxygen (O2) to produce water and nitric oxide (NO), how many moles of water are produced when 2.2 moles of ammonia are reacted?

Answers

Answer:

3.3 moles of H₂O.

Explanation:

We'll begin by writing the balanced equation for the reaction. This is illustrated below:

4NH₃ + 5O₂ —> 6H₂O + 4NO

From the balanced equation above,

4 moles of NH₃ reacted to produce 6 moles of H₂O.

Finally, we shall determine the number of mole of H₂O produced by the reaction of 2.2 moles of NH₃. This can be obtained as follow :

From the balanced equation above,

4 moles of NH₃ reacted to produce 6 moles of H₂O.

Therefore, 2.2 moles of NH₃ will react to produce = (2.2 × 6)/4 = 3.3 moles of H₂O.

Thus, 3.3 moles of H₂O were obtained from the reaction.

A cold air mass and a warm air mass are predicted to meet. What type of weather can be expected between the two air masses?
A.
warm weather
B.
cold weather
C.
dry weather
D.
stormy weather

Answers

Answer:

Explanation:

C. warm weather

Answer:

stormy weather

Describe the relationship between kinetic energy and the states of matter?


PLS HELP I DONT SCIENCE HELP ASAP HELP

Answers

Answer:

The kinetic molecular theory of matter states that: Matter is made up of particles that are constantly moving. All particles have energy, but the energy varies depending on the temperature the sample of matter is in. This in turn determines whether the substance exists in the solid, liquid, or gaseous state.

Explanation:

In an MO (molecular orbital) scheme for CH4, Group of answer choices ..there are three equivalent bonding MOs of equal energy and one unique bonding MO. ..there are four equivalent bonding MOs of equal energy. ..there are two sets of doubly degenerate bonding MOs. ..there are four bonding MOs, each of different energy.

Answers

Answer:

there are three equivalent bonding MOs of equal energy and one unique bonding MO.

Explanation:

Let us recall that according to the principles of the molecular orbital theory, when a compound is formed, the number of bonding molecular orbitals must be equal to the number of antibonding molecular orbitals.

Now methane is formed from one carbon atom and four hydrogen atoms. There are four bonding molecular orbitals and four antibonding molecular orbitals.

Three out of the four bonding molecular orbitals are degenerate while one is unique.

All drugs must interact specifically with a biological macromolecule to produce biological action؟
T /F​

Answers

Answer:

True

Explanation:

A drug is "a medicine or other substance which has a physiological effect when ingested or otherwise introduced into the body"(Oxford dictionary).

In the body, there are numerous macromolecules that perform various functions. These macromolecules often function as the target molecules to which different drugs may be bound.

A drug functions by binding strongly to a macromolecule with which it interacts in order to produce the desired biological action.

(The stands for a number the student is going to calculate.)

The ___________ stands for a number the student is going to calculate. Fill in the missing part of this equation.

(87. 1/mmole. C). ______= _____ kJ/mol.C

Answers

Answer:

1000 kJ.mmole / 1000 J.mole

Explanation:

To solve this, we need to analyze the given data.

We have a number which is 87.1 J/mmole.C (I'm assuming it has the J at the beggining because if not, then you are missing some data) and the final result is kJ/mol.C

The only unit that has not changed in the process was the °C, while the mole and J change respectively. In this case, we need to know the conversion factor of mmole to mole and J to kJ.

In the case of a mole:

1 mole --------> 1000 mmole

In the case of Joule:

1 kJ ----------> 1000 J

So the first thing we will do is to change from J to kJ:

87.1 J * 1 kJ / 1000 J = 0.0871 kJ

Now let's convert mmol to mole:

0.0871 kJ/mmole.C * 1000 mmole / 1 mole = 87.1 kJ/mole.C

As you can see, there's is practicly no change at all with the units, so putting all together it would be:

87.1 J/mmole.C * 1000 kJ.mmole / 1000 J.mole = 87.1 kJ/mole.°C

Hope this helps

Which statement defines the enthalpy of solution?
the total number of particles in a solution
the phase change from a solid to a liquid
o the difference in characteristics between reactants and products
the change in energy when one substance dissolves in another

Answers

Answer:

the change in energy when one substance dissolves in another

Explanation:

The enthalpy changes are the heat changes accompanying physical and chemical changes. Actually, an enthalpy change is the difference between the sum of the heat contents of products (final state) and sum of the heat contents of reactants (initial state).

There are basically two types of heat changes that accompanies are reaction which are:

Exothermic changes Endothermic changes

Therefore, the change in energy when one substance dissolves in another defines the enthalpy of solution.

Enthalpy of solution is the heat liberated or absorbed when one mole of a substance (solute) is dissolved in a specified volume of solvent (water).

Will measuring the melting point of your product tell you that your product is relatively pure?

Answers

Answer and Explanation:

Yes. The determination of melting point is used to analyze if a solid compound is pure or impure. The melting point of a pure solid product does not vary in a narrow range, whereas if impurities are present the solid melts in a broad range of temperatures. For this reason, this technique is generally used to determine the identity and purity of the products obtained in chemical reactions.

Which of the sets below are not in the order of lowest energy to highest energy: (A. 1s, 2s, 2p, 3s) (B. 2p, 3s, 3p, 4s) (C. 3s, 3p, 4s, 3d) (D. 3s, 3p, 3d, 4s)

Answers

Answer:

D

Explanation:

energy level order: 1s, 2s, 2p, 3s, 3p, 4s, 3d, 4p, 5s... (as periodic table shows)

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