Summarize at least 2 advantages of CRISPR.

Answers

Answer 1

CRISPR is simple and powerful compared to the rest of technologies used for editing genome. It is simple because it has the ability to point the definite DNA sequences which is facilitated by an easy to synthesize regulated strand of RNA. It is powerful because it let on an immediate target and remove and improve all deterioted DNA sequence present in a gene.

Answer 2

Answer: See Below

Explanation:

1. CRISPR utilizes a guide RNA (gRNA) that directs the function of a CRISPR protein effector to a specific target gene of choice, providing a versatile programmable platform. The targeting sequence of the gRNA is ~20bp therefore off-target genome editing is less common making it advantageous over most other restriction endonucleases.

2. CRISPR has increased versatility in many aspects and allows for a wide variety of applications in terms of genome editing. For example, The nickase version of the Cas protein induces only a single stranded cut thus opening up opportunities for altered editing. The Cas12a or Cpf1 version of the Cas protein cuts to form 5' sticky ends. And finally for the last example, the Cas protein has been bound to a reverse transcriptase and has shown efficacy in a technique called "Prime Editing" which has an altered gRNA that provides a primer binding site and template for full base insertions, deletions, and combinations of the two.


Related Questions

What’s the correct answer answer asap

Answers

Answer:

The answer is D. Jaw bone

Answers D I’m a mess student

13
Select the correct answer from each drop-down menu.
Vitamin B12 is important for the body to function. It helps keep nerve cells healthy, and it helps with the formation of red blood cells.
When someone is vitamin B12 deficient, one of the reasons could be that the person's
v is not
y the vitamin efficiently.

Answers

When someone is vitamin B12 deficient, one of the reasons could be that the person's dies does not contain proper amount of vitamin B12.

Is Vitamin B12 important?

A crucial B vitamin is vitamin B12. It is essential for the health of the brain, the generation of red blood cells, and nerve tissue. Vitamin B12 can also be called as cobalamin.

When the body cannot absorb enough vitamin B12, a deficiency or an insufficiency can develop. If left untreated, this can cause a variety of symptoms, including the development of irreparable brain problems.

Like every other B vitamin, this vitamin B12 is a water-soluble vitamin. This implies that it can dissolve in liquid and circulate through the body.

Up to four years’ worth of vitamin B12 can be stored by the body, and any extra or undesired amounts can be excreted in the urine.

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When do
resources
become a cause
of competition?
A. when a resource is mutated
B. when a resource is unlimited
C. when a resource is limited and being used by multiple
species

Answers

Answer: C. when a resource is limited and being used by multiple

species

Explanation:

Competing for Resources

Niche- the space, environmental

conditions, and resources

that a species needs in

order to survive and

reproduce

If niches overlap . . .

• Two or more species

rely on the same

limited resources.

• The result is

competition.

Competitive exclusion: one of

the competing species unable to

gain sufficient resources

How are damaged frontal lobes better if a younger person is injured in the frontal lobe than a 60-year-old man?

Answers

The frontal lobe damage would be greater to the 60-year-old man owing to the shrinking brain size.

What are the frontal lobes?

The brain is the organ that has the sole job of attempting and actually performing the task of trying to get all the parts of the body working effectively. he brain is a sort of administrator of the body. It the follows that the brain is the sole coordinator of the body and it is rightly located at the head.

Now there are several parts of the brain that are known to play various roles in the life of an individual. In this case, we can see that we talking about the frontal lobe located around the forehead and is very much impacted by an injury.

Owing to the fact that the brain shrinks with age, the older person would suffer a much higher memory injury compared to a younger person.

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2. Also submit a text-file, in which you explain in about one page what you have learned about the evolution of hominids. In the end, complete the following sentence: The notion that we evolved from modern apes (chimps) is clearly wrong. Rather, what we have evidence for is ....

Answers

The notion that we evolved from modern apes is wrong but we have evidence that humans evolved from apelike ancestors.

How did hominids evolve?

With time, genetic change can change the overall way of life of a specie like eating behavior, growth, and place of living. Human evolution took place when new genetic changes occur in the ancestor's populations which favor new abilities in that organisms to adapt to environmental change. Scientific evidence indicates that the physical and behavioral traits that are shared by all people come from apelike ancestors which evolved about six million years. One of the earliest defining human traits is bipedalism i.e. the ability to walk on two legs was evolved over 4 million years ago.

So we can conclude that hominids evolved because of the genetic change that occurs with time due to environmental change. We have many evidence that humans evolved from apelike ancestors due to environmental change.

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ATP --> ADP + P is an example of an _reaction,because there is __an
of the energy
energy.
endergonic; input
exergonic; input
exergonic; output
endergonic; output

Answers

ATP → ADP + P is an example of an endergonic reaction, because there is an output  of the energy.

An endergonic reaction is one that calls for free electricity to proceed. An example of an endergonic response of biological hobby is photosynthesis. Photosynthetic organisms conduct this response by using using sun photons to drive the discount of carbon dioxide to glucose and the oxidation of water to oxygen.

Within the exergonic response, the reactants are at a higher unfastened power degree than the goods reaction is going energetically downhill. In the endergonic response response, the reactants are at a decrease loose power stage than the products response goes energetically uphill.

The endergonic reactions of photosynthesis and chemosynthesis allow creatures at the bottom of the energy pyramid to live to tell the tale and to feed organisms like ourselves, who get their strength by means of breaking down sugars and fats to free up that stored power.

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You have been given a stock glucose solution with a concentration of 100mg/mL. To perform the glucose assay in lab today, you must dilute the 100mg/mL stock solution of glucose to concentrations of 80mg/mL, 60mg/mL, 40mg/mL, and 20mg/mL. You need to prepare 1mL of each dilution. Explain how you would make the 4 dilutions from the glucose stock solution.

Answers

In order to prepare 1 mL 80 mg/mL, 1 mL 60 mg/mL, 1 mL 40 mg/mL, and 1 mL 20 mg/mL glucose solution from a 100 mg/mL stock solution, 0.8 mL, 0.6 mL, 0.4 mL, and 0.2 mL of the stock will be taking and diluted with 0.2 mL, 0.4 mL, 0.6 mL, and 0.8 mL water resectively.

Dilution

The dilution principle states that the number of moles of solute in the original stock before dilution must be equal to the number of moles of solute in the diluted stock.

This principle is mathematically expressed as: m1v1 = m2v2

Where: m1 and v1 = molarity and volume before dilution

           m2 and v2 = molarity and volume after dilution.

In this case, the stock is 100 mg/ml.

To prepare 1 ml, 80 mg/mL solution: m1 = 100 mg/mL, v2 = 1 mL, and m2 = 80 mg/mL.

       v1 = v2m2/m1

           = 1x80/100

            = 0.8 mL

This means that 0.8 mL of the stock will be taken and diluted with 0.2 mL of water to make 1 mL, 80 mg/mL solution.

To prepare 60 mg/mL solution:

        v1 = 1 x 60/100

              = 0.6 mL

This means that 0.6 mL of the stock will be taken and diluted with 0.4 mL of water to make 1 mL, 60 mg/mL solution.

To prepare 40 mg/mL solution:

        v1 = 1 x 40/100

              = 0.4 mL

This means that 0.4 mL of the stock will be taken and diluted with 0.6 mL of water to make 1 mL, 40 mg/mL solution.

To prepare 20 mg/mL solution:

        v1 = 1 x 20/100

              = 0.2 mL

This means that 0.2 mL of the stock will be taken and diluted with 0.8 mL of water to make 1 mL, 20 mg/mL solution.

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How might you model the relationship between a tomato plant and the hornworm catepillar?

Answers

Answer:

Parasitism.

Explanation:

The relationship between a tomato plant and the hornworm caterpillar is parasitism because parasitism is a type of symbiotic relationship in which one organism get benefits and the other get harmed. We know that hornworm caterpillar feed on leaves and destroy the tomato crop. So it gets nutrients from tomato plant and the tomato plant is negatively affected. The input of this system is the vegetative part of tomato plant while the output is the conversion of hornworm caterpillar into a moth.

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What incentives does the government
offer to business who lower their
carbon emission and use alternative
energies?
A. free waste disposal
B. tax breaks
C. free alternative energy sources
D. more land to expand to

PLEASE HELP ME!

Answers

For qualifying renewable energy technologies and projects, the federal government offers a variety of tax credits, grants, and loan programs.

How does a tax credit for energy work?An energy tax credit is a government incentive that lowers the cost of alternative energy resources for individuals and businesses. Those who meet the criteria are reimbursed when they pay their income taxes, with the credit amount either reducing the total amount owed to the IRS or being added to their refund if one is due. In 2018, 2019, 2020, and 2021, an individual may claim a credit for Congress extended the ITC in August 2022, increasing it to 30% for installations between 2022 and 2032. 10% of the cost of qualified energy efficiency improvements and the amount of the taxpayer's residential energy property expenditures paid or incurred during the taxable year (subject to a $500 overall credit limit).

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What’s the correct answer asap for brainlist

Answers

pepsin : the chief digestive enzyme in the stomach, which breaks down proteins into polypeptides.

Which phrase best describes
nuclear fusion?
the spontaneous emission of radiation from an unstable nucleus
the process by which small nuclei
combine into a
larger nucleus
the process by which a large nucleus IS divided into smaller nuclei
d
series of reactions in which particles from one reaction trigger the next reaction

Answers

Answer: A larger nucleus

How would you ensure you have valid data?
PLS HELP ITS DOU TOMORROW!!
6TH GRADE SCIENCE

Answers

Answer:

To ensure valid data, you have to run it twice or three times and see if you get the same thing you got to before

What is the importance of knowing the 5R techniques?

Answers

Answer:

These R's include: reduce, reuse, recycle, repurpose and finally, refuse. This is an important methodology for businesses to follow to ensure they can reduce waste and boost their recycling efforts. This ultimately lessens the amount of waste that will end up in landfill and will optimise your recycling programs.

Explanation:

hope it's help:)

In tomatoes, red fruit (R) is dominant over yellow fruit (r). A plant that is homozygous for red fruit is crossed with a plant that has yellow fruit. List the possible genotypes and phenotypes for the offspring along with their percentages.

Answers

The possible genotypes and phenotypes for the offspring along with their percentages.

Homozygous individuals in diploid organisms (2n) carry two copies of the same gene variation (i.e., the same allele).Heterozygous individuals carry two alleles for the same gene locus.In total dominance, the dominant allele entirely covers the recessive allele.

This is further explained below.

What are phenotypes?

Generally, A collection of an organism's visible features or traits is referred to as its phenotype in the field of genetics.

The phrase refers to the physical shape and structure of the organism, as well as its morphology, its developmental processes, its biochemical and physiological qualities, its behavior, and consequences of behavior.

In conclusion, When a dominant red fruit (RR) plant is bred with a recessive over yellow fruit (RR), the phenotypic and genotype are Rr. Complete supremacy.

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In the exponential model of population growth, the growth rate remains constant. O keeps changing. O increases and then decreases. O increases.​

Answers

In exponential model of population, the growth rate remains constant regardless of population size.

What is population growth and how come the population growth in exponential form remains constant?Population growth simply means the growth of population , the growth in the amount of people holding a population.Population growth are of two types , logistic and exponential population growth .Population growth varies from the population of bacteria in culture plate, to the population of tiger to humans.Population growth can be measured by different means like population density , growth per capita.The exponential growth simply keeps constant  regardless of how fast the population is growing.

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Which statement describes how energy moves through an ecosystem?
OA. The Sun's energy is captured by primary consumers, and about
10% of the energy at each level is available to the next trophic
level.
OB. The Sun's energy is captured by producers, and about 90% of the
energy at each level is available to the next trophic level.
OC. The Sun's energy is captured by primary consumers, and about
90% of the energy at each level is available to the next trophic
level.
OD. The Sun's energy is captured by producers, and about 10% of the
energy at each level is available to the next trophic level.

Answers

[tex]\quad \huge \quad \quad \boxed{ \tt \:Answer }[/tex]

[tex]\texttt{D.) The sun's energy is captured by} [/tex] [tex] \texttt{producers, and about } [/tex][tex]{ 10% } [/tex][tex] \texttt{of the energy is available to} [/tex][tex] \texttt{the next trophic level. } [/tex]

____________________________________

[tex] \large \tt Solution \: : [/tex]

Producers are the ones who are responsible for making their own food, in this process they capture energy from sunlight. And as we move in the food chain, 10 of the whole energy is transferred to next trophic level, as most of the energy is consumed by the organism itself.

Answered by : ❝ AǫᴜᴀWɪᴢ ❞

Answer:

D. The suns energy is captured by producers, and about 10% of the energy at each level is available to the next trophic level.

Explanation:
Got Correct On ASSIST.

Monomers are small molecules that can join together to make larger molecules called polymers.
Nearly all of the large molecules in our bodies are a kind of polymer. Which of the examples below are monomers? Check all that apply
A.DNA
B.Starch
C.Sucrose
D.Amino Acid
E.Glucose

Answers

Glucose is the answer. I don’t know why I need 20 characters to submit this. But there you go. Good luck!

asexual reproduction

Answers

Answer:

Asexual Reproduction is the act of reproducing without the need of sexual intercourse

Examples:

Amoebas,Komodo Dragons,Starfish,Whiptail Lizards,Python Snakes

Your friend Elena beat her old weightlifting record yesterday, but today she woke up
barely able to move her legs. She says she feels a lot of pain whenever she bends her
legs. With this information, the most likely diagnosis would be:
passive muscle strain.
active muscle strain.
delayed onset muscle soreness.
acute muscle spasm.

Answers

The most likely diagnosis for this person who woke up barely able to move her legs would be delayed onset muscle soreness (option 3).

What is delayed onset muscle soreness?

The expression delayed onset muscle soreness makes reference to a condition in which a person exhibits limited muscle movement, which may be due to a different condition or also due to excessive physical exercise over a short period of time.

Therefore, we can conclude that delayed onset muscle soreness may be a condition that might emerge in an individual under excessive physical exercise.

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the distance between Earth and mars is 2.25Km. When converting using the conversion factor 1 AU=1.5 x 10 power 8km, the distance between the planets is__ . the astonomical unit AU is__ a light year

Answers

The complete statement is given as "The distance between Earth and mars is 2.25Km. When converting using the conversion factor 1 AU=1.5 x 10 power 8km, the distance between the planets is 1.5 AU."

This is further explained below.

What is an astronomical unit?

Generally, The astronomical unit is a measure of length that is equal to 8.3 light minutes or 150 million kilometers. It is approximately equivalent to the distance that separates the Earth and the Sun.

In conclusion, The distance from Earth to Mars is about 2.25 kilometers. Using the conversion factor that one AU is equal to 1.5 x 108 kilometers, the distance that separates the planets is calculated to be 1.5 AU.

The light-year and the AU are not interchangeable units of measurement. The distance traveled by light throughout one year is about 9.5 x 1012 kilometers.

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POSSIBLE POINTS 909
1) Fertilizers added to crops on farms can collect in runoff and enter the
watershed. This problem would be reduced in areas where
A
A) crops are grown for animals as well as humans.
B B) farms are situated on the sides of mountains.
CC) an extended drought leads to little or no rain.
D D) the growing season of the crops is longer.
1 2 3 4 5 6

Answers

Answer:

C

Explanation:

An extended drought leads to little to no rain.

Answer:

c

Explanation:

.

Cool air is less dense than warm air.

Question 7 options:
True
False

Answers

False. Think about how teachers have air conditioners on the top of the windows and how air balloons float up!! Therefore warm hair is lighter then cold air.

What factors led the red wolf to be considered extinct in the wild in the 1980s?

Answers

Answer:

human persecution and habitat loss

Explanation:

Which is not an abiotic factor?
A. soil
B. temperature
C. climate
D. trees

Answers

Answer:

Option D. should be correct

Explanation:

prove me wrong

Answer:D
Explanation: abiotic factors are non living and trees are living things

what are the two processes in meiosis that create genetic differences among cells?

Answers

Answer:

independent assortment during anaphase and crossing over during prophase

Explanation:

genetic material is swapped during crossing over

chromosomes from either mother or father could be put into any gamete during independent assortment

The two processes in meiosis that create genetic differences among cells are independent assortment and recombination.

What are recombination and independent assortment?

DNA fragments are broken and recombined during the recombination process to create novel allele combinations. The genetic variation that results from this recombination process at the gene level reflects variations in the DNA sequences of various species.

Recombinant and novel gene combinations are produced by the independent collection of genes.

The connected genes do not demonstrate independent assortment, since they are inherited together. More parental types and fewer recombinant are created because connected genes lack independent assortment.

Thus, the two processes are independent assortment and recombination.

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The spores that funguslike protists produce
A:
break down food for the protist
B:
produce slime on which the protist can move
C:
form pseudopods
D:
grow into new organisms

Answers

The answer to this question is D

Answer: D. grow into new organisms

A student decided to find the density of her house key to try and identify the type of metal from which it is made. She found the volume of the key to be 5 mL and the mass to be 39.5 g. Using the chart above, from which type of metal is the key made?

Answers

Going by the density of the key which is 7.9 g/mL, the key is likely made from iron.

Density

The density of a substance is the ratio of the mass of the substance and of its volume.

The density of a substance can be mathematically expressed as:

Density = mass/volume.

Considering the key in question whose volume is 5 mL and mass is 39.5 g. The density can be determined as:

Density of the key = 39.5 /5

                                 = 7.9 g/mL

In order to determine the nature of the metal that the key was made from, one would need to check the table of density of metals. Any metal whose density corresponds with, or is close to 7.9 g/mL would be the metal that the key is made from.

From a chart of density, the metal with a density of 7.9 g/mL is iron.

In other words, the key must have been made from iron.

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How does the temperature of water change if you heat it after it has alread
reached the boiling point?

Answers

The temperature of water does not change.

This is because when water is heated to its boiling point of 100 degrees Celsius, it usually takes more energy to turn a liquid into a gas, so the extra heat helps with that conversion. The temperature of water, even if it reaches its boiling point, does not change until all the water evaporates into the atmosphere. Depending on the type of container, the temperature will rise when the water runs out.

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How does an enzyme catalyze a chemical reaction?
A. It reduces the amount of energy needed for the reaction to begin.
B. It reacts with the substrate to form the product.
C. It makes a copy of the sequence of nitrogen bases in the
substrate.
D. It forms a layer of insulation that protects the substrate from the
cold.

Answers

Answer:

A

Explanation:

An enzyme does not alter a chemical reaction but shift so as to neutral it.

Option A is most suitable

What is a sugar apple?

Answers

Answer:

Ooh! Loving all these fruit questions, Anyways this is a great fruit. Sugar Apples or Sweetsop is native to the tropical Americas but is also widely grown in Pakistan, India, and the Philippines. The fruit looks a bit like a pine cone and is about 10 cm in diameter. Under the hard, lumpy skin is the fragrant, whitish flesh of the fruit, which covers several seeds inside, and has a slight custard taste.

Explanation:

Other Questions
Create a Prolong sentence and has at least two context clues help the worlds meaning Example 1: A gas is expanded from an initial volume of 20.5 L at 0.92 atm at room temperature (23.0C) to afinal volume of 340.6L. During this expansion, the gas cools to 12.0C. What is the new pressure? the acropolis played an integral role in athenian life. this hilltop not only housed the famous parthenon but it also included temples theaters and other public buildings that enhanced athenian culture how is the enduring issue topic of ideas and beliefs represented in this document? make a list below with evidence from the text:1.2.3. Ice has ________ energy. 1. mechanical 2. cold 3. heat Kendra spent $22 on 11 song downloads. How much did each song cost? 7. Falling objects drop with an acceleration of 9.8 m/s.If an Olympic high diver steps off the 10 m platform and falls for 1.4s,what velocity will she enter the water at? Given f(x) = 3(4-x), what is the value of f( 2)? Enter your answer in the answer box. jessica expereinece difficulty keeping her balance after receiving a blow to the back of her head. it is likely that she injured her a. medulla b. thalamus c. hypothalamus d. cerebellum d. cerebellum It is important that the conclusion of a speech restates the exact information that is in its introduction.TrueFalse A piston releases 18 J of heat into its surroundings while expanding from0.0002 m2 to 0.0006 m at a constant pressure of 1.0 * 105 Pa. What are thecorrect values for heat and work for the piston?A. Q = -18 J, W = +40 JB. Q = +18 J, W = +40 JC. Q = +18 J, W = -40 JD. Q = -18 J, W = -40 J Order these numbers from least to greatest 6.3071, 6.7 6.408, 6.47 which of the following was not an challenge experienced by the first settlers in south carolina Thinking about Traditions: (Female freedom and subjugation). Why was the feminism evident in Europe and the Americas less effective and applicable elsewhere? How did women in Asia and Africa experience both freedom and subjugation? Consider the reaction: 2Al + 3S = Al2S3If you have 45 grams of aluminum...a. How many grams of aluminum sulfide are produced? (Theoretical)b. When the rxn is performed in the lab, 114 grams of aluminum sulfide is produced. What is the percent yield? Based on lines 38-41, you can best make the inference that the pilgrims areO easygoingO cleverO sympatheticO compassionate helicobacter pylori gastritis is thought to be caused by a previous infection when the client was younger. chronic gastritis caused by h. pylori can lead to which possible condition? x =7, -2, 0, 7y= 10,0,10,3 Is this a function? please help me quickly Function g is a transformation of function f.-6 -54g(x) =-2 -1-1f(x)-2-3What is the equation of function g?-5-62 HELPPPP PLEASEEE!!!(MATH AND CHEMISTRY RELATED)