Replacing native gel electrophoresis with nonreducing sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) during the purification step results in eluates with no enzymatic activity. The best explanation of this observation would be:

Answers

Answer 1

Replacing native gel electrophoresis with SDS results in eluates with no enzymatic activity because it confers a negative charge to proteins, thereby neutralizing them.

What is sodium dodecyl sulfate?

The sodium dodecyl sulfate (SDS) is a detergent used in molecular biology laboratories.

This detergent (SDS) confers a negative charge to proteins, thereby neutralizing them (loss of their enzymatic activity).

In conclusion, SDS results in eluates with no enzymatic activity because this detergent may neutralize them.

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Related Questions

African fish eagles are large raptors that have a wide range across sub-Saharan Africa. Which of these organisms do you think would have a niche similar to that of an African fish eagle?
A- American bald eagle
B- European blue jay
C- White-tailed deer
D- Mountain lion

Answers

The organism that would have a niche similar to that of an African fish eagle is the American bald eagle (option A).

What is a niche?

A niche refers to a function within an ecological system to which an organism is especially suited.

The niche of an organism distinguishes that organism from other organisms and ensures its adaptation and survival.

According to this question, African fish eagles are large raptors that have a wide range across sub-Saharan Africa. They consume fishes mostly as preys.

This suggests that the organism that would have a niche similar to that of an African fish eagle is another organism with similar characteristics such as the American bald eagle.

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Select the correct answer from each drop-down menu.
Minerals are micronutrients required by the body for various key functions. The mineral BLANK
is required to carry oxygen in the
blood to all body tissues, and BLANK is a rich source of this mineral.

The mineral options:
Calcium
Iron
Sodium

The second blank:
Vegetable oil
Red meat
Milk

Answers

Blank 1 = iron..blank 2= red meat

Select the pituitary hormone that increases the concentration of blood sugar A. cortisol B. growth hormone C. glucagon AND. epinephrine E. insulin

Answers

The tragus is a small pointed eminence of the external ear, situated in front of the concha, and projecting backward over the meatus. It also is the name of hair growing at the entrance of the ear.

Use the meanings of the prefixes and suffixes in the table to form words that will match these definitions

Answers

In this question we use the meanings of the prefixes and suffixes in the table to form words that correspond to these definitions.

What is the form words that correspond to these definitions?

1. microbiology

2. zoology

3. hydrology

4. aquatic

5.microsheet

6. intracellular

7. microseed

8.phototropic

9. arthrosis

10. unicellular

1) bacteria

2) synthesizes the organic part of the cellular matrix

3) defense phagocytic cell

4)image magnification tool

5) true cells

6) order of genetic descendants

7) process of obtaining energy from plants

8) living beings that produce their own food

With this information, we can conclude that The prefix and suffix are morphemes, also called affixes, which are used with word stems to form a new word that takes on a new meaning.

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Which of Mendel's laws or principles states that gametes carry one allele for each trait?

Answers

Answer:

Law of Segregation: When gametes form, alleles are separated so that each gamete carries only one allele for each gene. Law of Independent Assortment: The segregation of alleles for one gene occurs independently of that of any other gene* Principle of Dominance: Recessive alleles will be masked by dominant alleles.

Explanation:
Law of Segregation: When gametes form, alleles are separated so that each gamete carries only one allele for each gene. Law of Independent Assortment: The segregation of alleles for one gene occurs independently to that of any other gene* Principle of Dominance: Recessive alleles will be masked by dominant alleles

In the CRISPR/Cas system __________. Multiple Choice restriction endonucleases digest viral genomes once they enter the cell if the viral DNA is not methylated bacteria integrate portions of the viral genome into their own so they have a molecular snapshot of their attacker to prevent future encounters methylated DNA sequences are hydrolyzed, effectively destroying the viral DNA methylase enzymes add methyl groups to the viral nucleic acids, preventing transcription

Answers

In the CRISPR/Cas system restriction endonucleases digest viral genomes once they enter the cell if the viral DNA is not methylated bacteria integrate portions of the viral genome into their own so they have a molecular snapshot of their attacker to prevent future encounters.

With CRISPR/Cas, it is simple to delete a gene of interest or, if a DNA template is included, to introduce a new sequence at the appropriate location. The technique has significantly shortened the time and cost required to create animal models with precise genomic alterations, which has had a significant impact on biomedical research. The CRISPR/Cas system is currently regularly used in mice for this reason by JAX researchers. Additionally, it is theoretically conceivable to implant DNA that corrects known mutations in human disorders like cystic fibrosis.

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The respiratory structure that contracts and relaxes to allow air to flow in and out of the lungs is the: Group of answer choices Larynx Epiglottis Diaphragm Alveoli

Answers

The respiratory structure that contracts and relaxes to allow air to flow in and out of the lungs is option(c) i.e, the diaphragm.

What is the diaphragm?

The diaphragm, a delicate skeletal muscle that separates the chest from the belly, is located at the base of the chest. When you breathe in, it flattens and shrinks. As a result, the lungs experience a vacuum effect that draws air in. The diaphragm relaxes and the air is forced out of the lungs when you exhale.

signs of diaphragm issues:

Acid reflux, heartburn, coughing, and difficulty swallowing.alterations to skin tone (skin may turn blue).rapid heartbeat, pressure in the chest, and/or breathing difficulties (especially when lying down).Headaches.Hiccups that persist or recur frequently.

Patients who need surgery for conditions affecting the chest, including diaphragm issues, are treated by a thoracic surgeon.

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If a population has relatively few individuals in the young classes, but many in the medium-aged and old classes, what can we conclude about population growth

Answers

This tells us that the population is declining.

What is a declining population?Reduction in population sizeRepresented by urn-shaped pyramidLow number of individuals in pre-reproductive age group (young), maximum number of individuals in reproductive group (medium-aged) and few number in post-reproductive group (old).

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the meninges along with the cerebrospinal fluid that circulates in between each meningeal layer function by

Answers

The meninges, along with the cerebrospinal fluid (C.S.F.) that circulates in between each meningeal layer, function by: serving as shock absorbers for the brain and spinal cord.

Meninges :

The meninges are the three membranes which envelop the brain and spinal cord and separate them from the walls of their bony cases (skull and vertebral column). Meninges are classified as either spinal meninges, which encase the spinal cord, or cranial meninges, which surround the brain.

The major function of the meninges is to protect the brain and spinal cord from mechanical stress, to support the blood vessels and to create a continuous canal through which cerebrospinal fluid (CSF) flows.  The innermost meningeal layers (arachnoid and pia), collectively known as the leptomeninges, are where the cerebrospinal fluid (CSF) travels.

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When a population grows in proportion to its current size (e.g., bigger populations grow faster), that population is experiencing ________ growth. exponential logistic capacity density-dependent density-independent

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The population is experiencing Exponential growth.

Exponential growth is a sample of records that shows a sharper will increase through the years. In finance, compounding creates exponential returns. savings bills with a compounding interest charge can show exponential growth.

The difference between geometric boom and exponential growth is, that geometric increase is discrete (due to the constant ratio) whereas exponential growth is continuous. With the geometric increase, a hard and fast range is increased to x while with an exponential increase, a hard and fast variety is raised to the x.

Exponential population increase is very lots crucial and vital to residing international as we all understand that modern-day populace worldwide is exceeding the wearing ability of earth due to its exponential growth that is ensuing in severe food disaster, water scarcity and economic fall down of the planet earth.

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If a person is HIV positive, why does he or she need to eat 10% more calories than
do his or her noninfected counterparts?

1. HIV-positive people have a high red blood cell count.
2. HIV-positive people have a low red blood count.
3. HIV-positive people use less energy.
4. HIV-positive people use more energy

Answers

HIV-positive people use more energy. Thus, option "D" is correct.

Why HIV-positive people use more energy?

These extra calories are needed by people with HIV because their immune system is constantly fighting off the HIV virus.

This is because HIV attempts to evade the infection even as the immune system attempts to eradicate it. The extra calories give the immune cells, which are extra strained, the energy to perform their functions.

Thus, option "D" is correct.

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The process of heredity discovered by Mendel filled the major gap in Darwin's theory of evolution, by identifying the source of the _________ found among the organisms of all species. This in turn provides the raw material from which natural selection 'selects.'

Answers

Answer: mutation...

Explanation: it's most likely mutation since it's what creates adaptation and adaptation helps different creatures in natural selection

Question 31 of 34
Which situation shows a nonrenewable resource in use?

Answers

Answer:

chips made from silicon

Explanation:

Non renewable resources are natural substances that cannot be replenished when used for eg natural gas, coal, petroleum ,fossil fuels etc. Silicon is made when silica is heated with carbon after which the the product is reacted with hydrocarbons to polymerize them. Since the hydrocarbons are made from natural gas, oil ,it is non renewable

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In the russian federation, which official holds the most power? a. the duma b. the president c. the prime minister d. the monarch please select the best answer from the choices provided a b c d

Answers

Answer:

B. The President

Explanation:

Answer:

He is right. Can I have brainlyest

Explanation:

3. Watson and Crick determined that DNA reproduced by a ______ Watson-Crick_____________________ model, in which the daughter molecules contain _________ of the parent strands and _______ newly synthesized strand.

Answers

Watson and Crick determined that DNA reproduced by a Semiconservative Watson-Crick model, in which the daughter molecules contain one of the parent strands and one newly synthesized strand.

According to Watson and Crick's theory, nitrogenous bases on opposing DNA strands form hydrogen bonds that hold the two strands of the double helix together. Each pair of bases forms a flat "rung" on the ladder-like structure of the DNA molecule. Base pairs don't just consist of any arrangement of bases.Watson and Crick proposed that DNA replicates in a "Semi-Conservative" way based on their understanding of the structure of DNA.According to this idea, the DNA double helix's two strands unwind and split, with each "parent" strand acting as a template for the creation of a new "daughter" strand.

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Identify and describe the stages of cell growth and reproduction in both plant and animal cells.

Answers

The main phases of the cell growth in plants and animals include:

InterphaseNuclear divisionCytokinesis

What is Cell division?

This is the process in which the parent cells divide into two or more daughter cells.

Plants and animals both undergo meiosis and mitosis with the former being used during reproduction while the latter is used during growth etc.

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Hi you can help me? pls i dont know

Answers

Answer:

U can search on Internet.

All of these are available on internet

Several blood vessels of the human body contain receptors that sense when the blood pressure is too high. When these receptors are stretched (high blood pressure), they cause the slowing of the heartbeat and the widening of vessels to bring the pressure down to normal levels. This scenario is an example of a homeostatic mechanism that operates by: negative feedback. resource cycling. noncompetitive inhibition. competitive inhibition. positive feedback.

Answers

Several blood vessels of the human body contain receptors that sense when the blood pressure is too high. When these receptors are stretched (high blood pressure), they cause the slowing of the heartbeat and the widening of vessels to bring the pressure down to normal levels. This scenario is an example of a negative feedback.

A response that is the opposite of the change noticed constitutes negative feedback (it functions to reduce the change)As a result of a change being sensed by a receptor and an effector being triggered to produce the opposite action, equilibrium is promoted.Homeostatic systems, such as the following, are examples of processes that use negative feedback loops:Thermoregulation (if body temperature changes, mechanisms are induced to restore normal levels)Control of blood sugar (insulin lowers blood glucose when levels are high ; glucagon raises blood glucose when levels are low)Osmoregulation (ADH is secreted to retain water when dehydrated and its release is inhibited when the body is hydrated)The control of blood pressure is an illustration of a negative feedback loop. Receptors in the brain can recognize a rise in blood pressure.

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Several blood vessels of the human body contain receptors that sense when the blood pressure is too high. When these receptors are stretched (high blood pressure), they cause the slowing of the heartbeat and the widening of vessels to bring the pressure down to normal levels. This scenario is an example of negative feedback.

What is negative feedback?

Negative feedback is a response that is the exact opposite of the change observed (it functions to reduce the change)

Equilibrium is encouraged when a change is perceived by a receptor and an effector is activated to take the opposite action.

Negative feedback loops are employed by homeostatic systems, such as the following:

Thermoregulation (if body temperature changes, mechanisms are induced to restore normal levels)management of blood sugar (insulin lowers blood glucose when levels are high ; glucagon raises blood glucose when levels are low)Osmoregulation (ADH is secreted to retain water when dehydrated and its release is inhibited when the body is hydrated).A negative feedback loop is demonstrated by controlling blood pressure. A rise in blood pressure can be detected by brain receptors.

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Attachment of the mitotic spindle fibers to the kinetochores is a characteristic of which stage of mitosis?.

Answers

Attachment of the mitotic spindle fibers to the kinetochores is a characteristic of the Metaphase stage of mitosis.

The main stages of mitosis are as follows:

Prophase: During prophase, the chromatin material begins to condense into visible chromosomes.Metaphase: During metaphase, the chromosomes line up at the equator attached to spindle fibers. The mitotic spindle fibers attach to the kinetochores of the chromosomes.Anaphase:  During anaphase, the chromosomes are pulled apart and homologous chromosomes separate and move to opposite poles. Telophase and cytokinesis: During telophase nuclear membrane begins to appear and two distinct nuclear membranes are visible.

hence metaphase is the correct answer.

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Cells in the thyroid gland produce and secrete thyroid hormone (a polymer of amino acids) that helps to regulate metabolism. What organelle is most likely abundant in cells of the thyroid gland?

Answers

Rough Endoplasmic Reticulum (RER) organelle is most probably prevalent in thyroid gland cells.

Rough Endoplasmic Reticulum (RER)RER is a cell organelle that is in charge of protein synthesis as well as the cellular transport of protein-containing vesicles.It consists of cisternae, tubules, and vesicles. The cisternae are comprised of flattened membrane plates that participate in polypeptide processing.Ribosomes, which are protein production sites, wrap the rough endoplasmic reticulum (RER) membranes.The RER produces the majority of enzymes and cell membrane proteins.RER is abundant in organs and cells which release enzymes, including the pancreas.

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what is the produt of meilosis 1

Answers

Answer:

Explanation:

Meiosis one results in 2 haploid cells (duplicated), meanwhile meiosis two results in 4 haploid cells that are not doubled.4

1. Describe the
overall changes in
matter that occur
during the light-
independent
reactions. What does
the plant start with
and what does it end
with? What is the
source of energy that
powers this change?

Answers

Answer:

takes place in the stroma of the chloroplast. Carbon dioxide is modified by series of chemical reactions to form carbohydrates. The energy for these reactions comes from ATP and NADPH generated during the light dependent process.

Explanation:

N/A

A high tide will occur A usually only once in a 24-hour period B only in bodies of water close to the moon C on both sides of the earth at the same time D when proximity to the sun makes the days longer

Answers

A high tide will occur on both sides of the Earth at the same time, the correct option is C.

What is a high tide?

The tidal force causes Earth and its water to bulge out on the sides closest to and farthest from the moon. These water bulges are high tides.

The Moon's gravitational pull is pulling on the Earth and the ocean on the sides facing the Moon. The Earth compensates for the Moon's pulling by bulging out both toward and away from it.

Thus, the correct option is C.

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Dna is more resistant to hydrolysis than rna. What feature of dna is responsible for this characteristic?.

Answers

The presence of a 2'-hydroxyl group in RNA

Deoxyribose does not include a 2′-hydroxyl group. The reason RNA is more susceptible to hydrolysis is because it has a 2'-hydroxyl group. The 3'-phosphate may be attacked by the 2'-OH, which might cause the phosphodiester link to be hydrolyzed.

A newly discovered species of bird is found to only lay a single egg and both parents are necessary to raise the chick to adulthood. What would be the most successful mating strategy for this species

Answers

monogamous is the answer.

When birds are vigilant, they rub together the swollen cloaca. The male sperm stored in his cloaca deposits in the female cloaca, where it travels up the chamber and eventually fertilizes the egg.

Most birds mate for only one season and for one reason. However, some species mate for life, while others mate multiple times during the season. Goose, swan, and eagle are known to have only one companion until one of them dies.

Monogamous is a mating system in which one adult male and one adult female are mated. Such a pair of turns can follow a single breeding attempt, breeding season, or many breeding seasons, such as mating mammals, geese, and swans.

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Hello,what is meant by the term Biome as used in ecosystem.


Answers

an area classified according to the species that live in that location is known as Biome...

Example= Desert.

A biome is a large, naturally occurring ecosystem which has a generally routine climate throughout the year and houses a certain group of species whose niche fits the climate!

How many different types of gametes could an organism with the genotype aabbcc produce?.

Answers

Answer:

Thanks

Explanation:

In the ovary, oocytes are held at metaphase II of meiosis until fertilization. How is this block released

Answers

In the ovary, oocytes are held at metaphase II of meiosis until fertilization.This block released, when Progesterone activates c-Mos, which further phosphorylates maturation promotion factor (MPF).

Maturation promoting factor :

One of the major protein kinases that activates and acts as the master regulator for the M-phase transition is Cdk1/cyclin B, also known as maturation promoting factor or MPF. It phosphorylates and activates other downstream protein kinases and directly phosphorylates several structural proteins involved in cellular reorganization.

It also  play a major role in chromatin condensation by phosphorylating condensins; a role in NEBD by phosphorylating lamins, nuclear pore complexes, and inner nuclear membrane proteins; a role in fragmentation of the Golgi apparatus by phosphorylating Golgi matrix proteins; and a role in spindle formation by phosphorylating centrosome-associated proteins and MAPs.

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Why is determining the AVR important

Answers

Since 1996, Atmel has created the AVR family of microcontrollers, which Microchip Technology purchased in 2016. As contrast to the one-time programmable ROM, EPROM, or EEPROM used by other microcontroller families at the time, AVR was one of the first to utilise on-chip flash memory for program storage.

One of the most important rain forest predators, attacking a wide range of different primates, is the monkey-eating eagle. We know this because

Answers

one of the most important rain forest predators, attacking a wide range of different primates, is the monkey-eating eagle. we know this because all forest primates have an identical alarm call that means, essentially, "Eagle!"

What are primates?

Primates are those forest animals that has highly developed brain and backbone.

Some of them are predators while others are source of food for predators known as preys.

Example of such predators are the monkey eating eagles which are capable of hunting monkeys as their preys.

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an elevator has a maximum load restriction of 2.5 tons. is it safe for two tile layers weighing 175 ib and 240 ib plus 80 boxes of tile weighing 50 ib each We need to build a model for a category Y for a binary classification problem (with categories of: 0,1) and having one binary attribute X1 (with possible values of: 0,1 per attribute).The Nave Bayes algorithm was chosen for training the classification model.After the training phase, we got the following results:p(X1=0|Y=0)=0.35, p(X1=1|Y=0)=0.65p(X1=0|Y=1)=0.3, p(X1=1|Y=1)=0.7p(Y=0)=0.5, p(Y=1)=0.5Which of the following answers is correct?Select the best answer Select one:A.If the category Y=1, we will classify the example as X1=1B.If the value of X1 is 0 we will classify the example as category Y=0C.If the value of X1 is 1 we will classify the example as category Y=0D.None of the answers is correct, since we're missing the probabilities: p(X1=0), p(X1=1), Hat Tricks Company (HTC) is a Buffalo, New York, manufacturer of hats and gloves. Recently, the company purchased a new machine to aid in producing the hat product lines. Production efficiency on the new machine increases with the workforce experience. It has been shown that as cumulative output on the new machine increases, average labor time per unit decreases up to the production of at least 3,200 units. As HTCs cumulative output doubles from a base of 100 units produced, the cumulative average labor time per unit declines by a learning rate of 80%. HTC has developed a new style of mens hat to be produced on the new machine. One hundred of these hats can be produced in a total of 20 labor hours. All other direct costs to produce each hat are $19 per hat, excluding direct labor cost. Direct labor cost per hour is $60. Fixed costs are $8,000 per month, and HTC has the capacity to produce 3,200 hats per month. Controls are designed to mitigate risk and provide reasonable assurance about the achievement of an organization objectives with respect to. What is Relative Dating?A. None of theseB. The science of determining the order of events in relation to each other, without a specific date.C. The science of determining how long ago a specific ancestor lived.D. The science of using radioactive isotopes to determine the specific age of a sample. CAN SOMEONE HELP PLEASE a = 3, b = 5, and c = 7. What is m&B to the nearest tenth? In order to compute the area of a particular circle, Juan first measures the length of its diameter. The actual diameter is 20 cm, but Juan's measurement has an error of up to $20\%$. What is the largest possible percent error, in percent, in Juan's computed area of the circle what is the range of f(x)=3^x 50 POINTS HELP NOWFind the logarithm base 10 of each number: 10 Anyone interested in weighting a proposal that describes a solution to improve the electoral process in Zimbabwe? which model best shows the movement of water during photosynthesis . A housing down payment is money that aprospective buyer provides up front whenpurchasing a home and is usually a per-cent of the purchase price of the home. Alender typically requires private mortgageinsurance (PMI) when the buyer's downpayment is less than 20% of the purchaseprice. To secure a mortgage, buyers alsoneed to have additional cash on handfor closing costs and prepaid propertytax. Suppose a buyer wants to purchasea $375,000 house and must have $7,200on hand for closing costs and propertytax. Which of the following inequalitiesrepresents the total funds (f) the buyermust have on hand to secure the mortgagewithout having to pay PMI?A) f0.2(375,000) + 7,200B) f0.2(375,000) +7,200C) f0.2(375,000 +7,200)D) f0.2(375,000 +7,200) Which two gases were part of both stage 2 and stage 3 in the formation of the atmosphere? I need help with this question I got 1 as an answer but its saying its wronglog 4 (x +255) = 4 Find a function f such that f '(x) = 5x3 and the line 40x + y = 0 is tangent to the graph of f. Which of the following is the harm for a host organism in parasitism?1. Nourishment and development of parasites2. Acquiring new genes or characters from the parasites3. Developing new body parts for the parasites4. Increasing the parasite According to the rare Earth hypothesis, the conditions that support life on Earth are unique, and the odds of similar conditions being found on another planet are close to zero. In contrast, the mediocrity principle holds that our planet, solar system, and galaxy are utterly ordinary, making it likely that life is common elsewhere.Which is the most effective topic sentence for the paragraph? What type of triangle is shown below?3128.519.210940%991 For the following exercises, find the x- and y-intercepts of the graphs of each function.26. f(x) = 4| x 3 |+ 4 Texas's tax structure is one of the most regressive in the country. This is due in part to its ______.