Protein phosphatase 1 (PP1) catalyzes a reaction which yields products that regulate cell division; consequently, PP1 is a possible drug target to treat certain types of cancers. The PP1 enzyme cata- lyzes the hydrolysis of a phosphate group from myelin basic protein (MBP). The reaction is shown below:
PP1 MBP-phosphate-→ MBP+P,
The activity of PP1 was measured in the presence and absence of the inhibitor phosphatidic acid (PA). The concentration of PA was 300 nM. vo without PA vo with PA released . (mg/mL) mL"1 . min1) mL-1 . min-1) 0.00381 0.00620 0.00931 0.0140 0.010 0.0209 0.0335 0.0419 0.0838 0.015 0.025 0.050
(a) Use the data provided to construct a Lineweaver-Burk plot for the PP1 enzyme in the presence and absence of PA. What kind of inhibitor is PA?
(b) Report the KM and Vmax values for PP1 in the presence and absence of the inhibitor.

Answers

Answer 1

Answer:

Explanation:8iikk


Related Questions

Please help me anyone ?!!!! ())).

Answers

Rusting of iron and cutting an onion are both examples of chemical changes.

How to explain the chemical change

Rusting of iron is a chemical change because it involves a reaction between iron, oxygen from the air, and water. The iron reacts with oxygen to form iron oxide, commonly known as rust. This process is irreversible, and the chemical composition of the iron is altered during rusting.

Cutting an onion is also a chemical change because it involves the disruption of the onion's cell structure and the release of enzymes. When you cut an onion, enzymes stored within the cells are released, reacting with certain compounds to produce sulfur compounds. These compounds are responsible for the pungent smell and the tears that occur when cutting an onion. The cutting process causes a chemical transformation in the onion's composition, making it a chemical change.

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What tonicity term applies to the solution surrounding this cell?

Answers

Hypotonic. Since the black molecules can cross the cell membrane and water molecules are able to cross, the solution surrounding the cell has a lower solute concentration compared to the inside, causing water to move into the cell.

To determine the tonicity of the solution surrounding the cell in the given image, we need to consider the movement of water molecules across the cell membrane.

Based on the image, the black molecules can cross the cell membrane, while water molecules are also able to cross. Tonicity refers to the concentration of solutes outside the cell compared to inside the cell.

In this case, if the solution surrounding the cell has a lower concentration of solutes compared to the inside of the cell, water will move into the cell to equalize the concentrations. This would cause the cell to swell or potentially burst.

Therefore, the appropriate tonicity term for this situation is "Hypotonic." A hypotonic solution has a lower concentration of solutes compared to the inside of the cell, resulting in water moving into the cell.

Hence, the correct answer is "Hypotonic."

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A solid is that form of matter that possesses the following
characteristics __.
a
Particles vibrate around fixed axes
b Definite shape
C
Definite volume
d All of the above

Answers

Definite volume is doesn’t possess a definite volume so therefore the answer is c

Wave frequency is measured in units
called Hertz (Hz). One Hertz is defined as
one wave per second. Suppose a person
counted 10 waves passing by over a time
period of 1 second. What is the wave
frequency?

Answers

Answer:

it will be 10Hz ,because number of waves per second is 10 so we divide with 1second

The wave frequency of a wave which is passing by 10 waves over a time period of 1 second will be 10 Hertz (Hz).

What is Wave frequency?

Wave frequency can be defined as the number of waves which are passing through a fixed point in a given amount of time period. The SI unit for the wave frequency is the hertz (Hz), where 1 hertz is equal to 1 wave which is passing through a fixed point in the time period of 1 second. A higher-frequency wave has generally more energy than a lower-frequency wave with the same magnitude of amplitude.

The wave frequency of a wave which is counted to be 10 waves passing through a point in a time period of 1 second will be 10 Hertz (Hz).

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Identify the two genes of each parent that would always produce an ear of corn which has all yellow kernels.

Answers

It would be YY or Yy

there are several types of practical sciences activities. tabulate the characteristics of demonstrations , experiments and practical investigations. information should include the following:the role of the learner during the practical activities, the role of the teacher and the important element of the practical activity to consider ?​

Answers

Demonstrations:

Learner's Role: Observes and analyzes the demonstration.Teacher's Role: Demonstrates the concept or process.Important Element: Clear explanation by the teacher.

Experiments:

Learner's Role: Conducts experiments, manipulates variables, and records data.Teacher's Role: Guides the experimental process.Important Element: Controlled variables for reliable results.

Practical Investigations:

Learner's Role: Conducts independent investigations, formulates research questions, and analyzes results.Teacher's Role: Facilitates the investigation process.Important Element: Clear research question to guide the investigation.

Here's is  the characteristics of demonstrations, experiments, and practical investigations in practical science activities:

Demonstrations:

Learner's Role: Observes the demonstration, paying attention to key concepts and outcomes.Teacher's Role: Demonstrates the concept or process, explaining the steps and providing clear explanations.Important Element: A clear explanation by the teacher is crucial to ensure learners understand the demonstrated concept or process.

Experiments:

Learner's Role: Actively conducts experiments, manipulating variables, making observations, and recording data.Teacher's Role: Guides the experimental process, providing instructions, explaining concepts, and ensuring safety.Important Element: Controlled variables are crucial in experiments, as they help maintain consistency and reliability of results.

Practical Investigations:

Learner's Role: Conducts independent investigations, formulating research questions, collecting data, and analyzing results.Teacher's Role: Facilitates the investigation process, providing guidance, resources, and support.Important Element: The formulation of a clear research question is crucial in practical investigations, as it guides the entire investigative process and ensures focused outcomes.

In summary, demonstrations involve learners observing and teachers explaining, experiments involve learners actively conducting experiments with guidance from teachers, and practical investigations involve learners conducting independent investigations with support from teachers. Clear explanations, controlled variables, and focused research questions are important elements in each activity, respectively.

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How does the DNA in the offspring produced by these methods compare to the DNA in the original
organism?
A) The offspring contain half the original number of chromosomes in each method.
B) The DNA in the offspring is genetically identical to that of the original organism in both methods.
C) The offspring produced by method A contain twice the original number of genes, while those
produced by method B contain half the original number of genes.

Answers

The DNA in the offspring is genetically identical to that of the original organism in both methods.

What is DNA?

In both methods, the offspring produced would have DNA that is genetically identical to the original organism. This is because both methods mentioned do not involve any form of genetic recombination or mixing of genetic material.

The offspring are produced either through asexual reproduction (method A) or by cloning (method B), where the genetic material is replicated without any changes or variations. As a result, the DNA sequence in the offspring remains identical to that of the origin

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Which was present in the unknown? starch lipids nucleic acids proteins​

Answers

To test for the presence of starch, you can use iodine solution, which turns blue-black in the presence of starch. Lipids can be identified using a Sudan III or Sudan IV test, where lipids will appear as red or orange-stained spots.

Nucleic acids can be detected through tests like the diphenylamine test or the biuret test.

The diphenylamine test produces a blue color in the presence of DNA, while the biuret test results in a purple color in the presence of RNA.

Proteins can also be identified using the biuret test, as proteins will cause the solution to turn purple.

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PLEASE HELP!!!
what protein is part of the basal lamina outside blood vessels, and activates platelets when they stick to it?
-hemoglobin
-collagen
-fibrinogen
-tissue factor​

Answers

Tissue factor, I think.

**100 POINTS** DNA is an organic molecule that is the code of life. DNA is made up of smaller molecules that form together into two chains. Those chains spiral around each other to form the DNA molecule. Which dichotomous key best describes DNA?

Answers

Answer:

Each step is based on genetic traits

Explanation:

so I think u can choose which one it is based on wht i said

Examine the diagram of the nitrogen cycle.
Which option best describes what's happening in the part
labeled 3?
Ammonia, nitrates, and other compounds dissolve in
water and are carried to the oceans.
Human activities add nitrates and other compounds
to Earth's systems.
O Nitrogen is stored in the largest reservoir on Earth.
Nitrogen-fixing bacteria convert nitrogen gas into
compounds usable by plants and animals.

Answers

The part labelled 3 shows nitrogen is stored in the largest reservoir on Earth.

What is the nitrogen cycle?

The transformation and cycling of nitrogen in various forms throughout ecosystems is referred to as the nitrogen cycle, a biogeochemical process. The nitrogen cycle involves the transformation of nitrogen between various chemical forms so that it is available for use by plants and other creatures.

Nitrogen is a crucial element for the growth and development of living beings.

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Why was Copernicus's heliocentric model not believed until Galileo and Kepler provided mo
The model had the moon orbiting Earth.
The model was against religious teachings.
The model could not explain retrograde motion.
The model did not include all eight planets in the solar system.

Answers

Answer:

the model was against religious beliefs.

How many please anyone answer for me?!!?!

Answers

In balanced chemical reactions, the type and amount of elements (atoms) is the same in reactants and in products. Considering the balanced reaction, in the product there is only one carbon atom (Option A- 1), and there must be four hydrogen atoms (Option D- 4).

What is a balanced chemical reaction?

A balanced chemical reaction is an equation in which the number and type of atoms are the same on both sides of the arrows.  

Following the law of mass conservation, chemical equations should be balanced to ensure that all the reactants appear in the products.

This means that no new atoms appear, and no atoms disappear. The only thing that changes in a chemical reaction is the bond between atoms.

So each side of the equation must be equilibrated concerning the amount and type of atoms.

Coefficients are used to balance reactions. These numbers are located before each atom and indicate the number of units present in the formula.

In the exposed example, we need to look at the coefficient and the atoms to know how many if them are present.

1)

CH₄ + O₂ ⇄ CO₂ + H₂O

Reactants

1 Carbon4 Hydrogens2 Oxygens

Product

1 carbon3 oxygens2 hydrogen

This eqution is not in equilibrium. To balance it, we need to add coefficients

CH₄ + 2 O₂ ⇄ CO₂ + 2 H₂O

Reactants

1 Carbon4 Hydrogens4 Oxygens

Product

1 carbon4 oxygens4 hydrogen

Considering the balanced reaction,

In the product there is only one carbon atom. Option A) 1In the product there must be four hydrogen atoms. Option D) 4

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7. Phytoplankton + Shrimp → Snapper → Shark
There are 2,500 kcal available for the 1st trophic
level.
There are kcal available for the 2nd trophic level.
There are kcal available for the 3rd trophic level.
There are 2.5 kcal available for the 4th trophic level.
Fill in the organisms and energy amounts on the
pyramid to the right
8. Use the food chain you created in #3 and complete

Answers

Answer:

The first level is 2,500

The second is 250

The third level is 25

and the fourth level is 2.5

Explanation:

The amount of energy at each trophic level decreases as it moves through an ecosystem. As little as 10 percent of the energy at any trophic level is transferred to the next level; the rest is lost largely through metabolic processes as heat.

Am I supposed to answer #8?

The amount of energy at each trophic level decreases as it moves through an ecosystem. As little as 10 percent of the energy at any trophic level is transferred to the next level; the rest is lost largely through metabolic processes as heat.

What is an ecosystem?

An ecosystem consists of all the organisms and the physical environment with which they interact. These biotic and abiotic components are linked together through nutrient cycles and energy flows.

Moreover, 7 major ecosystems are forest ecosystem, grassland ecosystem, tundra ecosystem, desert ecosystem, freshwater ecosystem, and marine ecosystem.

Hence, healthy ecosystems clean our water, purify our air, maintain our soil, regulate the climate, recycle nutrients and provide us with food. They provide raw materials and resources for medicines and other purposes. They are at the foundation of all civilization and sustain our economies.

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Where is the greater solute concentration?

Answers

Answer:

outside the cell BH is in the

Answer:

B. Inside the cell

Explanation:

there are more black molecules inside the cell than anywhere else therefore there is a greater concentration inside the cell.

1.What is the different between pulmonary circulation and systematic circulation
2. What is the different between aerobic andanaerobic respiration?
3. What is the important of ATP?
4. Write the group blood lack of atigen and antibody respectively?
5. Why the left ventricles thicker than right ventricles?
6. What is the different between disinfectants and antiseptics?
7. Write the life cycle of tape worm?
8. What are the main differences between cholera and typhoid?
9. Write the reason for need classification.
10. Write the genus name and species name of Homosapiens respectively ​

Answers

1. We can see here that the difference between pulmonary circulation and systematic circulation:

Pulmonary circulation is the circulation of blood from the heart to the lungs and back to the heart. While systemic circulation is the circulation of blood from the heart to the rest of the body and back to the heart.

What is aerobic and anaerobic respiration?

2. Aerobic respiration is a type of respiration that requires oxygen. While anaerobic respiration is a type of respiration that does not require oxygen.

3. The importance of ATP is that it is used to power all of the body's cells, including muscle cells, brain cells, and heart cells.

4. The group blood lack of atigen and antibody:

Blood group   Antibodies        Antigens

A                                Anti-B            A

B                                Anti-A                  B

AB                                None         A and B

5. The left ventricle is thicker than the right ventricle because it has to pump blood to a larger area of the body.

6. The difference between disinfectants and antiseptics is that:

Disinfectants kill microorganisms on surfaces, while antiseptics kill microorganisms on living tissue.

7. The life cycle of a tapeworm begins when an infected animal sheds tapeworm eggs in its feces. If a human ingests these eggs, they hatch in the small intestine and the larvae burrow through the intestinal wall and travel to the liver, lungs, or brain. In these organs, the larvae mature into adult tapeworms.

8. The main differences between cholera and typhoid are:

Cholera is caused by the bacterium Vibrio cholerae, while typhoid is caused by the bacterium Salmonella typhi. Cholera is spread through contaminated water, while typhoid is spread through contaminated food or water. Cholera symptoms typically develop within hours of infection, while typhoid symptoms typically develop within days of infection.

9. The genus name for H0mo sapiens is H0mo and the species name is sapiens.

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 Which would be worse a change in DNA or a change in mRNA & why

Answers

Answer:

i think its DNA

Explanation:

im not sure

explain the following type of respiration with diagram in toad:
1..Buccal respiration
2..Cutaneous respiration
3.. Pulmonary respiration​

Answers

Answer:

1.Buccal pumping is "breathing with one's cheeks": a method of ventilation used in respiration in which the animal moves the floor of its mouth in a rhythmic manner that is externally apparent.

2.Cutaneous respiration, or cutaneous gas exchange, is a form of respiration in which gas exchange occurs across the skin or outer integument of an organism rather than gills or lungs.

3.Pulmonary ventilation is commonly referred to as breathing. It is the process of air flowing into the lungs during inspiration (inhalation) and out of the lungs during expiration (exhalation.

Yan Lang po Sana makatulung


6. What two places might a free-floating DA molecule end up?

Answers

is there any answer choices before i start answering ur question

Cellular respiration and photosynthesis are similar in that both require
O A. glucose
B. membrane proteins
C. oxygen
O D. carbon dioxide
UBMIT
12

Answers

Answer:

If you can choose 2 your answers would be A.Glucose and C.Oxygen but if not try Oxygen.

Which part of a seed acts as the food source for the seed as it grows underground?

Answers

The answer is a cotyledon

(12.02 MC)
Why are spines considered a physical characteristic adaptation in cactus?

Answers

white dense spines help reflect sunlight spines provide shade cacti stem is thick and fleshy to store lots of water stem has waxy waterproof coating to help keep water in the cacti

1. As countries develop, do they have more or less of an impact on the planet? Explain.

2. What is biocapacity?

3. In worldmapper.org, where is the highest level of poverty, population, and hunger in the world?

4. Look at the US for income and resource use from the video Human Population Impacts by Bozeman Science. Did the US grow larger or smaller in size? How do you think this impacts the environment?

5. If the economy is too big, does it have a negative or positive effect on the environment?

Answers

1. The ever-growing development of nations accelerates their impact on Earth, as they consume more resources and energy and heighten their industrial and urban expansion. It`s worth noting that even though developed countries have a greater environmental impact, developing countries also face substantial ecological hurdles as they aim for growth and expansion.

2. Biocapacity refers to the ability of an ecosystem or region to support human populations by providing resources such as food, water, and energy, as well as absorbing waste and pollution.


3. According to worldmapper.org, the highest level of poverty is found in sub-Saharan Africa, particularly in countries such as Niger, Central African Republic, and Chad. The highest population levels are found in Asia, particularly in China and India.


4. According to the video ‘‘Human Population Impacts‘‘ by Bozeman Science, the US has grown larger in size both in terms of population and resource use over time.

5. The impact of a large economy on the environment depends on how it is managed and regulated. While a large economy can provide opportunities for innovation and investment in sustainable technologies, it can also lead to increased consumption and production that can have negative environmental impacts.

Search using the internet on how you can expand or enhance one's knowledge of animal/fish raising.​

Answers

Answer:

Fish farming involves raising a variety of aquatic animals, such as fish, mollusks and shrimps in an enclosure such as a tank or pond. With the surge in demand for healthy animal protein the  demand for fish and fish products is on the rise. Fish farms have the potential to produce fish for home consumption at about $0.90/lb which is a considerable saving when compared to supermarket prices of over $2.00/lb.

The first step for fish farming is to determine what you are going to do with your full-grown fish. You need to know the purpose, whether you are going to keep fish as a pet or for fish meat. This will decide a lot of things like the space requirements, equipment and capital requirements. If you intend to keep fish as a pet for showcase then a small tank or a backyard small fish farm is sufficient. If you intend to put a small fish farm with one or two ponds then also with a little business mindset you can market 1000 to 2000 lb of fish.  

This will also require you to set up a business entity and have a business mindset.The costs associated with raising different types of fish varies because different fish need certain temperature water to live. The type of fish also decides the requirement of equipment and the feed cost. There are lots of laws about raising fish and animals, and even more, stringent laws related to raising animals that are going to be consumed as food.

Before starting fish farms or fish hatchery of any size or type it is advisable to check local laws and the need of permits  to do so in your area.Normally you will get a license to operate your fish farm for a year which will be renewable. You need to submit the following documents to apply for the license:

Filled in application form

Sketch or aerial map of the facility layout, including dimensions

Copy of fish health certificate

Copy of stocking permit

Letter of a written permission from the landowner (in case if you are not the landowner)

Explanation

(I truly apologize if this answer is too long)

inquiry based pedagogy is being embraced in principle across the globe. in the last decade , it has been supported by an increasing body of research on its effectiveness. illustrate your understanding of the concept of inquiry based pedagogy with a relevant example​

Answers

Inquiry-based pedagogy involves fostering curiosity and active learning. For example, students investigating real-world environmental issues and proposing solutions.

Inquiry-based pedagogy is a teaching approach that promotes active learning and critical thinking through inquiry and exploration. One relevant example of inquiry-based pedagogy is a science classroom where students are encouraged to design and conduct their own experiments. Instead of simply following instructions, students formulate research questions, develop hypotheses, plan and execute experiments, collect and analyze data, and draw conclusions. For instance, students may investigate the effect of different variables on plant growth by manipulating factors like light, water, or soil composition. This approach allows students to engage in authentic scientific practices, develop problem-solving skills, and gain a deeper understanding of the scientific method. By actively participating in their learning process, students develop a sense of ownership and become more engaged, fostering a love for learning and nurturing their ability to explore and discover knowledge independently.

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Discuss the importance of the change in CO with exercise?

Answers

Enhancing cardiac output allows you to maintain lower heart rates during physical activity. During exercise SV will increase related to the response of the increased pressure of the heart in order to supply blood to the body.

Explain the difference between a data table and a graph.
When do you use a data table?
When do you use a graph?
HELP ME!!!

Answers

Answer:

A data table is a visual instrument comprised of labeled columns and rows, while a graph is a diagram showing the relation between variable quantities, typically of two variables, each measured along one of a pair of axes at right angles.

They are used for different purposes, depending on the problem

A data table is a visual instrument comprised of labeled columns and rows, while a graph is a diagram showing the relation between variable quantities, typically of two variables, each measured along one of a pair of axes at right angles.

Why would a rough, steep mountain habitat have a higher rate of speciation
than a large, open grasslands habitat? I will make u brainliest
A. The mountain has less variety in the environment.
B. The mountain would cause less fragmentation.
O C. The mountain has wider range of environments.
O D. The mountain has more resources.
SUBMIT

Answers

Answer:

I think the correct answer is A

Explanation:

Answer: C. The mountain has wider range of environments.

Marianne is conducting an experiment on homeostasis in paramecium, which is a type of single-felled organism. Which of these observations provides the strongest evidence of a paramecium maintaining homeostasis

Answers

Hello. You did not present the answer options, which makes it difficult for you to receive a specific answer. However, I will try to help you in the best possible way.

One of the most common evidences that a paramecium is maintaining homeostasis is the pumping of water from the vacuoles present in its body.

The paramecium is a protozoan that lives in aquatic environments, mainly those composed of fresh water. The problem with this environment is that water is able to enter the paramecium organism in large quantities and this can promote the disruption of the organism through lysis of the cell. In this case, homeostasis must act quickly and pump water out of the body, through the cell vacuole.

Homeostasis is a regulatory system of the organism, which must promote the balance of the organism, whenever any external or internal factor tries to harm this balance.

Please help me ASAP thank you

Answers

1. the sun 2. decomposers 3. i’m not sure 4. photosynthesis
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