Primer design is a critical step for the detection of a particular sequence in a sample. Which of the following would be true of primer design?

Answers

Answer 1

The group of choices is as follow:

A. RNA polymerase are added to the PCR mix to design random primers  

B. If detecting viral infection by PCR, the primers must not be complimentary (match) to regions of human DNA  

C. Pairs of primers must be designed, one to start amplification with Taq polymerase, the other to start amplification with human polymerase  

D. Primers are optional if you want to amplify all DNA within a sample

Answer:

The correct answer is - B. If detecting viral infection by PCR, the primers must be complementary to regions of human DNA.

Explanation:

Short, single-stranded segments of DNA sequences utilize in the real-time PCR or polymerase chain reaction technique are called primers. Primers used to hybridize the region of the segment needed to be amplified.

The human polymerase enzyme is not used in the PCR reaction and requires a 3'OH group to synthesize the DNA by amplification which is possible only in the presence of primers as they have a 3'OH.


Related Questions

1) Phloem is composed of two type of cells


2) Epidermal cells are

Answers

Answer:

1) Phloem is made of two types of cell parenchyma cells and schlerenchyma cells in adiition to it it also has sieve elements

2)The epidermis is the outermost cell layer of the plant body. Epidermal cells are tightly packed together thus provide mechanical strength and protection to the plant also helps in Transpiration and photosynthesis as it has special opening called stomatal openings

The basic unit of structure and function of living things is the?

Answers

Answer:

cell units

Explanation:

Answer:

Cell is the basic structural and functional unit of life.

how are underwater ecosystems affected by tidal waves

Answers

Answer:

A tidal wave is a shallow water wave caused by the gravitational interactions between the Sun, Moon, and Earth. ("Tidal wave" was used in earlier times to describe what we now call a tsunami.) A tsunamis is an ocean wave triggered by large earthquakes that occur near or under the ocean,...

i think

Match each muscle of the scapular with the correct label.

Answers

Answer:

Explanation:

1. Supraspinatus

2. Subscapularis

3. Infraspinatus

4. Teres major

What allows oxygen to create polar molecules?

Answers

Answer:

That the oxygen atom attracts electrons a little more strongly than hydrogen does. So even though the electrons from each atom are attracted by both the oxygen and the hydrogen, the electrons are a bit more attracted to the oxygen.

5. Besides being part of the composition of bone, why else do mammals need calcium?

Answers

Answer:

It's important for the contracting muscle

Explanation:

Besides being part of the composition of bones, calcium is needed by mammals for the following processes:

Formation of skeletons and teeth.Muscle contraction.Regulation of normal heart rate and nerve functions. Blood clotting.

What is Calcium in living organisms?

Calcium in living organisms may be defined as an important nutrient that all living organisms require, including humans in order to develop various organs like teeth and bones as well as for metabolic processes.

Apart from being part of the composition of bones, calcium is also required for the proper development of teeth and skeletons. The movement of calcium ions plays an essential role in the contraction of muscles.

The influx of calcium ions into the cells of the heart significantly enhances the heart rate of an individual in the range of 1.6 to 14.10 mm.

It also plays a critical role in blood clotting.

Therefore, it is well described above.

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Using this image as a reminder, which phrase gives two processes scientists think
move the Earth's lithospheric plates?

A. gravity acting on the edges of plates and friction between the plate and the asthenosphere

B. friction between the plate and the asthenosphere and pressure of magma on the
edge of the plate

C. gravity acting on the edges of plates and convection in the mantle

D. convection in the mantle and pressure of magma on the edge of the plate

Answers

Answer:

C. gravity acting on the edges of plates and convection in the mantle

Explanation:

A tectonic plate (also called lithospheric plate) is a massive, irregularly shaped slab of solid rock, generally composed of both continental and oceanic lithosphere. Therefore, the option (C) is correct.

What are lithospheric plates?

Plate tectonics is the generally accepted scientific theory that considers the Earth's lithosphere to comprise a number of large tectonic plates which have been slowly moving since about 3.4 billion years ago.

Earth's crust, called the lithosphere, consists of 15 to 20 moving tectonic plates. The plates can be thought of like pieces of a cracked shell that rest on the hot, molten rock of Earth's mantle and fit snugly against one another.

In plate tectonics, Earth's outermost layer, or lithosphere—made up of the crust and upper mantle—is broken into large rocky plates. These plates lie on top of a partially molten layer of rock called the asthenosphere.

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50 points and brainliest'!!!!!!!!!!!!!!!!!!!!!!!!
Reliability and probability in forensic tests are important. When you look at matching a paint chip to a specific car, you are working to increase the probability that the right car matching your paint chip evidence will be found. Reliability means you have practiced and applied the correct procedures and processes for all your evidence testing, and you know you can trust the results. Some forensic tests are more reliable than others, and the best type of test or tests to use will usually depend on the kind of evidence that is present. If all types of evidence were present at a crime scene, which type of test would you choose based on its reliability and probability of accuracy? Identify which test you would choose and provide a detailed argument as to why you feel it has a high level of reliability in comparison to other types of evidence. Your answer should be at least two paragraphs in length and should include solid arguments for your choice. There is no wrong choice, just be sure to provide a convincing argument.

Answers

Answer:

Explanation:

everything must match up inorder to find anyone accountiable

I need help I already started on some of it but I am stuck please help I would appreciate it all you have to do is draw a line from one bubble to the other look at the picture for an example thank you so much!

Answers

here you go! hopefully you can tell which lines go where (also i crossed out on of the lines in white because it was wrong haha)

is it true that all organisms are composed of one or more cells, and the life processes of metabolism and heredity occur within these cells.

Answers

Answer:

Yes it is true

Explanation:

Cells are the basic unit of all organisms

Which type of force is a contact force?

A. Gravity
B. Electromagnetic force
C. Friction
D. Strong nuclear force​

Answers

Friction is the answer

Answer:

Friction

A-P-E-X

A tree growing on a cliff is slowly growing its roots into the rocks and causing it to crack. What is this an example of? Why?
(28 Points)

Answers

Answer:

because the tree is merged into the cliff, so its because of erosion

Explanation:

Select the correct answer.

Macy always thought there were only a few hair colors: blond, brown, and black. However, when she actually began looking around, she saw varying shades of these hair colors. What is a possible reason for so many different hair colors?

Answers

Answer:

My guess is that the answer is genetics

Explanation:

polygenic inheritance

Can you identify the class of organic molecule to which these molecular structures and examples belong?Sort these items into the appropriate bins.

I SOLVED IT LOL

Answers

good job i am very. proud.

The correct class of molecules to which molecular structures and examples are:

Carbohydrates- starch and monosaccharide.Lipids - cholesterol and fatty acid moleculeProtein - amino acids and lactaseNucleic acids - DNA and nucleotide

1. Carbohydrates:  

Components: made of one or more monosaccharides.Functions: regulates energy for cell, energy storage, and plant cell support Examples: glucose, glycogen, starch and cellulose  

2. Lipids:

Components: fatty acid, glycerol, fat moleculeFunctions: energy storage, cell membrane components, and hormonesExamples: fats, phospholipids, and steroids  

3. Proteins:  

Components: amino group, carboxyl group, R group, and amino acidFunction: enzyme, structural protein, transport, communication, immunity, storageExample: lactose, hair, tendons, muscles, hemoglobin, signal proteins, antibodies, egg albumin, receptor protein  

4. Nucleic acids:  

Components: phosphate group, nitrogeneous base, ribose or deoxyribose, nucleotideFunctions: heredity  Example: code for proteins, DNA, RNA

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Match the following groups with the correct description

a. Euglenozoans
b. Diatoms
c. Brown algae
d. Apicomplexans
e. Green algae

1. exists as a large plasmodium and sporulates when conditions dry up.
2. are key primary producers. Have silica skeletons and produce oil to maintain buoyancy.
3. no cell wall, but pellicle. Some free living, others are parasites.
4. exhibits alternation of generations and produces both motile zoospores and sperm.
5. May be unicellular or colonial. Photoautotrophic and biflagellated
6. No cell wall, but have alveoli and a complex life cycle. Some are pathogens of humans.

Answers

Answer:

a- 3

b- 2

c- 4

d- 6

e- 5

Explanation:

A) Euglenozoans are eukaryotic organisms that belong to the Kingdom Protista. They are either free-living or parasitic in nature. They have no cell wall in their structure but instead possess a pellicle.

B) Diatoms are photosynthetic algae that belong to the division-Bacillariophyta. The ability to photosynthesize makes them a primary producer in an ecosystem. Their cell wall is made up of silica, which is unique to their group of algae. They also produce oils to maintain buoyancy.

C) Brown algae belong to the division- Phaeophyta. They exhibit alternation of generations in their life cycle where they produce both motile zoospores and sperm.

D) Apicomplexans are organisms such as plasmodium etc that belong to the phylum- Apicomplexa. Most of them are pathogenic, with some affecting humans. They lack cell wall but have a flat vesicle called ALVEOLI.

E) Green algae belong to the division Chlorophyta. They are photosynthetic organisms and exists as unicellular or colonial organisms. They have flagellated for locomotion.

A cross was made between two strains of plants that are agriculturally important. One strain was disease-resistant but herbicide-sensitive; the other strain was disease-sensitive but herbicide- resistant. A plant breeder crossed the two plants and then allowed the F1 generation to self- fertilize. The following data were obtained:
F1 generation: All offspring are disease-sensitive and herbicide-resistant
F2 generation: 157 disease-sensitive, herbicide-resistant
57 disease-sensitive, herbicide-sensitive
54 disease-resistant, herbicide-resistant
20 disease-resistant, herbicide-sensitive
Total: 288 Formulate a hypothesis that you think is consistent with the observed data. Test the goodness of fit between the data and your hypothesis using a chi square test. Explain what the chi square results mean.

Answers

Answer:

Hypothesis: Alleles assort independently from each other.There is not enough evidence to reject the null hypothesis.

Explanation:

Due to technical problems, you will find the complete answer and explanation in the attached file.        

A reduction of the amount of product of a particular gene is observed due to a mutation. This type of a mutation is called ____

Answers

I think genetic mutation or cell mutation ;,,) not sure !!

Water pollution control came about as a result of _______.
a.
public protest of the condition of the Cuyahoga River
b.
the Cuyahoga River fire of 1969
c.
the California oil spill
d.
environmental assessments

Answers

Answer:

B. the Cuyahoga River fire of 1969

Explanation:

i just took the test

Water pollution control came about as a result of the Cuyahoga River fire of 1969.

What do you mean by Water pollution?

Water pollution may be defined as the accumulation of undesirable substances in the water which make water harmful to living beings.

During the Cuyahoga River fire of 1969, the number of fire stations along the river doubled due to the fire that caught in the sewage water.

Therefore, water pollution control came about as a result of the Cuyahoga River fire of 1969.

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moon phase that is half light and half dark​

Answers

Answer:

Because a half of the half illuminated Moon can be seen, this is referred to as a "quarter Moon". When the Moon is waxing and reaches this position, it's called the "first quarter Moon"; when waning, the "third quarter Moon."

Orbits: Earth

Explanation:

Answer:

First Quarter Moon

Explanation:....

Marissa pedals her tricycle down the sidewalk. At what age was she likely first able to pedal? (2 points)

A.One year old
B.Two years old
C.Three years old
D.Four years old

Answers

Answer:

three years old i think. sorry if its wrong

Most likely three because they walk better and stuff

HELPPP ASAP! TIMED! 10 MINUTES LEFTT! How is cold front formation different from stationary front formation?
A.Stationary fronts form between warm and cold air masses, while cold fronts form when a cold air mass advances
against a warm air mass.
B.Cold fronts form between two air masses that barely move, while stationary fronts form when a warm air mass is
trapped between two cold air masses.
C.Cold fronts form when a warm air mass moves over a cold air mass, while stationary air fronts form when a cold
air mass moves over a warm air mass.
D.Stationary fronts form when a warm air mass is trapped between two cold air masses, while cold fronts form when
a warm air mass moves over a cold air mass.

Answers

Answer: Stationary fronts form between warm and cold air masses, while cold fronts form when a cold air mass advances against a warm air mass. Cold fronts form between two air masses that barely move, while stationary fronts form when a warm air mass is trapped between two cold air masses.

Explanation:

Each diagram shows a cell with a semipermeable membrane submerged in a
solution. Solutions contain different amounts of solutes (glucose, sodium). The
membrane is NOT permeable to glucose or sodium.

Answers

Explanation:

I guess I don't understand your question.

there are six classes of nutrients that must be considered when planning your diet: water, proteins, carbohydrates, fats, vitamins, and minerals. most foods or ingredients include more than one of the six basic nutrients. for example, although most people usually think of corn as containing carbohydrates, it is important to remember that a kernel of corn contains all six basic nutrients.

Match the words to the appropriate blanks in the sentences.

_______nutrients be synthesized by the human body, but are required for optimal functioning. Therefore. they must be Obtained from food. Omega-3 fatty acids are prime examples.
You consume macronutrients (water. proteins, carbohydrates, and fats) in large quantities as opposed to _________ which you consume in small quantities
_________ can develop very quickly when exercising in hot/humid weather conditions.
_________are also called 'body builders" due to their function within the body________ are the building blocks of proteins.

1. glucose and fructose
2. essential
3. incomplete
4. proteins
5. fat soluble
6. plant
7. amino acids
8.saturated fats
9. micronutrients
10. dehydration

Answers

Answer:

Essential nutrients cannot be synthesized by the human body but are required for optimal functioning. Therefore. They must be obtained from food. Omega-3 fatty acids are prime examples.

You consume macronutrients (water. proteins, carbohydrates, and fats) in large quantities as opposed to micronutrients which you consume in small quantities

Dehydration can develop very quickly when exercising in hot/humid weather conditions.

Proteins are also called 'bodybuilders" due to their function within the body. Amino acids are the building blocks of proteins.

Explanation:

Essential nutrients are those nutrients that are required for the proper functioning of the body but cannot be synthesized by the body.  They are obtained from external sources such as vegetables or synthesized artificially in the laboratories. Micronutrients as the name implies are taken in small amounts. Examples are the different types of vitamins.  

Dehydration occurs when a person loses a good amount of water through sweating or urination. It can easily occur while exercising in hot weather conditions. Proteins repair the cells in our body and are, therefore, rightfully referred to as bodybuilders. Amino acids form proteins.  

What is a major obstacle in cellular respiration? It is limited by the low amount of energy stored in a glucose molecule It is an exergonic reaction, with much of the energy lost as heat, which potentially might damage cells It is an inefficient reaction and doesn’t provide eukaryotic cells with enough energy It requires oxygen to be actively pumped against its concentration gradient into cells

Answers

Answer:

It is an exergonic reaction, with much of the energy lost as heat, which potentially might damage cells

Explanation:

Cellular respiration is an important process carried out by the cells of living organisms in order to supply energy needed for their various cellular functions. Cellular respiration generally involves the breakdown of the stored energy in glucose molecules (in the presence of absence of oxygen).

However, since the process of cellular respiration releases energy as chemical energy (ATP) and majority as heat energy, it is said to be an EXERGONIC REACTION i.e energy-releasing. This hear energy released can damage living cells, which is a major setback to cellular respiration.

A plant growing toward the sun is an example of

Answers

Answer:

phototropism

Explanation:

Answer:

Hi Noya here! (◕ᴥ◕)

Explanation:

Phototropism is the term that describes the growth of a plant toward any light source. School-age children often demonstrate this principle by showing how a newly sprouted seed, for example, leans in the direction of a nearby light bulb. Heliotropism is the process that describes how a plant specifically tracks the sun’s movement across the sky.

-happy to help! ʕ·ᴥ· ʔ

Looking at the following organisms and their diets, place them into a food chain. Make sure you have arrows that show the correct flow of energy.

Aardvark: omnivore diet
Ant: herbivore diet
Grass: autotroph
Hyena: carnivore diet

Answers

Answer:

Grass > Ant > Aardvark  > Hyena

Explanation:

The grass is autotrophic, which means it can produce its own food It does so using water, carbon dioxide, and light energy from the sun. The organisms in this food chain rely on other organisms for food.

The ant is a herbivore that consumes the grass for energy. The aardvark consumes both plants and animals (omnivore), so it can consume the ants. The hyena is a carnivore, meaning it only eats animals, such as the aardvark.  

What are the major parts of a cell

Answers

Answer:

Cell membraneNucleusCytoplasm

I hope this helps!

Answer:

Nucleus, nucleolus, ribosomes cell membrane, cytoplasm, mitochondria et cetra.

Answer for 25 points also I’ll mark you as the brainiest ! For the quickest answer.

Answers

Answer:

The Fourth Point (between 4 and 5)

You find the optimal pH by looking for the pH with the highest activity.

Explanation:

The optimal ph is basically where the enzyme has THE MOST activity.

The fourth point of the pH, and activity is the optimal pH because it has the most activity out of the nine points.

111. Sodium's atomic number is 11. What does this tell you about an atom of sodium?
a) It contains 11 protons.
b) It contains 11 neutrons.
c) Its atomic mass is double this amount.
d) It has 11 protons to lose in ionic bonds.

Answers

11 protons a I would say

A STRAND OF DNA IS 19% GUANINE. WHAT
PERCENTAGE IS CYTOSINE?

Answers

Answer:

28% cytosine

Explanation:Since cytosine and guanine are present in equal amounts, we can simply divide their sum by 2.  28% cytosine, and 28% guanine.

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