Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (KD) Distance Migrated (mm) 37 23 25 30 20 35 15 42 51.5 10 Distance Migrated (mm) vs. Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (kD) 40 30 Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight 20 10 25 30 35 40 45 50 Distance Migrated (mm) STUDENT MANUAL POST-LAB ACTIVITY Procedures Generate a standard curve to calculate protein sizes The different protein bands in your gel can be defined by their different molecular masses. Indeed many proteins are named for their molecular weights. For example p53, a protein implicated in tumor progression is 53 KD in size. To determine the molecular masses of the proteins, a standard curve is created plotting the known molecular masses of the proteins in the Precision Plus Protein Kaleidoscope prestained standards against the distance they have migrated down the gel from the base of the well. A 15% polyacrylamide gel is designed to separate small proteins-proteins less than 40 KD. Your gel analysis will concentrate on this size range. Note: If a different percentage acrylamide gel or an agarose gel has been run, analyze the section of the gel that has the best separation 1. As shown in the figure below draw a line between the 37 and 25 KD bands of the prestained standards. Your gel analysis will be restricted to the proteins below this line. Stenders Actin & Myosin 4 Measure distance from base of wells to the base of the bands 250 150 100 75 50 37 25 20 Measure Standard bands between 37 and 10 kD 15 Measure fish protein bands between 30 and 10 KD 10 - Fig. 18. Image of fish muscle proteins separated by SDS-PAGE and stained with Bio-Safe Coomassie stain. Lines illustrate measurement of bands for constructing the standard curve. STUDENT MANUAL POST-LAB ACTIVITY Define the characteristics (proteins) of the different fish 4. For each fish sample that has been analyzed, determine the molecular masses of the proteins below the 25-37 KD line. Measure the distance each band has migrated from the base of its well. Find that distance on the x-axis of the standard curve. Draw a line up from the x-axis to the curve. Read across to the y-axis to determine the molecular mass. Alternatively, use graphing software to generate the standard curve. Make a line of best fit (or trend line) through the points and formulate an equation to calculate the mass of the unknown proteins on the gel. 5. Enter this data into a table with the molecular masses of the proteins for each fish (see example below). Fish Species A Distance Molecular Migrated Mass (mm) (KD) 25 32.5 26.5 31 29 28.6 36 21.7 21.2 36.5 39 18.8 44 13.9 52 6 Determine which fish have each characteristic (protein) 6. Make a table with a row for every band size you have recorded for all your fish samples and a column for each type of fish on your gel. Then make a mark in each cell of the table where the fish has that size band (see example below). -LAB ACTIVITY ENT MANUAL Species A Species B Species c Species D Species E х X х х X х X х X х 27.5 X х х х X х X X X X 33 X х х X X х х X х Characteristic х Protein Distance Molecular Migrated Mass (mm) (kDa) 25 32.5 26 31.5 26.5 31.0 30.0 28.5 29.1 29 28.6 30 27.6 30.5 27.1 32 25.6 24.7 34.5 23.2 35.5 22.2 36 21.7 36.5 21.2 37 20.7 37.5 20.2 38 19.7 38.5 19.3 39 18.8 39.5 18.3 40.5 17.3 41 16.8 41.5 16.3 42 15.8 43 14.8 44 13.9 45 12.9 46 11.9 46.5 11.4 47 10.9 47.5 10.4 51.5 6.5 52 COUNT х х X X X х х х X х х х х х х х X х X X х X 6.0 х 8 10 13 10 12 Find the number of characteristics shared by each of the fish 7. In the table below both the row and column headings are the types of fish. From the table above, separately compare the number of bands (X's) in common with every other fish sample from your gel and put those numbers into the table below, such that each fish is individually compared with every other fish. In this example, species A and B have just 2 bands in common while species B and C have 10 bands in common. Your table will be the basis for drawing your cladogram. Species A Species B Species c Species D Species E Species A 8 2 2 2 Species B 10 10 5 3 Species C 13 5 Species D 2 Species E

Answers

Answer 1

Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (KD) Distance Migrated (mm).

What is the process of procdures generation?

Procedures Generate a standard curve to calculate protein sizes The different protein bands in your gel can be defined by their different molecular masses. Indeed many proteins are named for their molecular weights. For example p53, a protein implicated in tumor progression is 53 KD in size.  

To determine the molecular masses of the proteins, a standard curve is created plotting the known molecular masses of the proteins in the Precision Plus Protein Kaleidoscope prestained standards against the distance they have migrated down the gel from the base of the well.

Therefore, Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (KD) Distance Migrated (mm).

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Can someone help me

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Answer:

1...c

2... indicator

3... corrosive

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Which statement correctly describes these broken pieces of the sample

Answers

Each piece contains the same number of atoms." The statement that correctly describes these broken pieces of the sample is that each piece contains the same number of atoms.

Ruiz Instructions Question 6 1 pts The type of photoceptor in the eye that responds to color is the
O pupil
rod cells
O retina

cone cells​

Answers

Answer:

its retina respond to colour and form inverted image behind

HELP!!! 50PTS!!! WILL BE MARKED AS BRAINLIEST!!!!

Which of the following is a consequence of populations with little genetic diversity?
Ability to withstand environmental pressures
Risk of decline or extinction
Protection against spread of disease
Increased reproductive success

Answers

Answer:  1 people would be more vulnerable to disease

2 human or anything wouldnt live as long.  

or if this a b c d choice it would  risk of decline or extinction  

Explanation:

NEED HELP ASAP

What situation most likely explains the occasional high frequency of certain inherited disorders among human populations established by a small population?

Bottleneck effect, increasing the gene pool of the population
Mutations resulting in neutral changes to genotype
Gene flow and migration of populations of diverse individuals
Founder effect, due to a limited number of individuals from a parent population

Answers

Answer: Founder effect, due to a limited number of individuals from a parent population brainliest?

Explanation:

Answer:

The founder effect is genetic drift attributable to colonization by a limited number of individuals from a parent population.

What might happen to plants if they were unable to develop parts of the stem underground?

Answers

Answer:

The water travelling from the roots to the leaves will slow and eventually stop… ... If there are no buds, the plant my become deceased unless there is bulb or root mechanism that will resprout vegetative growth from below the ground to start a new plant…

Explanation:

Hope this helps

red marrow tissue interacts with which 2 body systems

Answers

Answer:

I believe it interacts with the lymphatic system (because it creates lymphocytes) and the circulatory system (because it creates red blood cells).

- The situation in which atmospheric gases trap the sun's heat and keep Earth's
surface warm is called
a. weather.
c. climate.
b. greenhouse effect.
d. primary succession.

Answers

Answer:

b. greenhouse effect.

Explanation:


Answer:

Greenhouse effect

Compare the characteristics of mammals & flowering plants'

Answers

Answer:

they are both the same

Explanation:

science

Compare the two Food Web. Which ecosystem would be more likely to survive if a disease killed the grass?

a The forest ecosystem, because the animals would be able to eat other organisms.
b The grassland ecosystem, because it has many herbivores.
c he grass ecosystem, because several predators compete for food
d The forest ecosystem, because it has 3 top predators

Answers

Answer:

A

Explanation:

just A, I remember doing this in like 4th or 3rd grade don't really remember but I makes sense


2. Full-sized wings are the typical phenotype for Drosophila. Which of the following events most likely led to the
existence of the short-wing allele in Drosophila?
A. a change in the shape of a chromosome
B. a change in the DNA sequence of a gene
C. the insertion of a DNA strand during mitosis
D. the deletion of a chromosome during meiosis

Answers

Answer:

B.  A change in the DNA sequence of a gene

Explanation:

HELP ASAP!!!!!!! PLEASE !!!!


Which property of water allows water to be resistant to sudden temperature changes?
Choose 1 answer:
A) Surface tension
B) Density
C) Adhesión
D) High specific heat capacity

Answers

Answer:

your answer will be (A) surface tension

Answer:

High Specific Heat Capacity

Explanation:

What type of organism really spread and diversified during the Cenozoic?
reptiles
fish
amphibians
mammals

Answers

D) mammals really diversified during the Cenozoic era

Mammals really spread and diversified during the Cenozoic era. So, the correct option is D.

What was Cenozoic era?

The Cenozoic Era is defined as the third of the major eras in Earth's history that began about 66 million years ago and extends to the present. This epoch was the interval of time during which the continents assumed their modern configurations and geographic positions during which the Earth's flora and fauna evolved towards the present.

The Cenozoic Era is called the "Age of the Mammals", while the typical landscapes of North America began to develop during the Cenozoic. Birds and mammals rose to prominence after the extinction of giant reptiles.

Thus, Mammals really spread and diversified during the Cenozoic era. So, the correct option is D.

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Please help will give brainliest

Answers

Explanation:

Species

Genus

Family

Order

Class

Phylum

Kingdom

Domain

Is this an accurate description of the water cycle?

The water cycle is water in the sea or ocean that gets evaporated, that’s condensation. It turns into clouds but then just falls again as rain/snow, that’s precipitation. It goes to streams and runoffs. That is the water cycle.

Answers

Answer:

Yes,I think this is correct

Answer:

right, maybe that's the right answer

Explanation:

sorry if my answer is wrong

PLEASE BE SPECIFIC THANK YOU <3
Due to its location, Hawaii does not have very many land birds. The Hawaiian honeycreepers are a favorite for many ornithologists. There are over 50 species of honeycreepers that can all be traced back to one ancestor. The level of diversity in an ecosystem can be determined by the frequency of speciation and extinction.

Describe speciation and identify two possible prezygotic barriers of the Hawaiian honeycreepers that contribute to speciation.
If the Hawaiian honeycreepers became extinct, explain how the newly available niches could lead to speciation.

Answers

Answer:

In speciation, organisms evolved with the passage of time.

Explanation:

Speciation is the evolutionary process in which populations of species evolve with the passage of time to become different species from the original one.  Temporal isolation and gametic isolation are two possible prezygotic barriers of the Hawaiian honeycreepers that contribute to occur speciation. The newly available niches could lead to speciation of that organisms that tolerate the Hawaiian climatic conditions when the extinction of Hawaiian honeycreepers occurs. The new organisms used the available resources such as food and space etc and increase its population.

what is a transgenic organism?
a. an organism that is an exact clone
b. an organism that has a mixture of DNA
c. an organism that has no DNA
d. an organism with DNA from another organism

Answers

I think is a
Look it up

DNA is copied during the S Phase of Interphase.  Why do cells copy the DNA?

Answers

Answer:

During mitosis, a eukaryotic cell undergoes a carefully coordinated nuclear division that results in the formation of two genetically identical daughter cells. Then, at a critical point during interphase (called the S phase), the cell duplicates its chromosomes and ensures its systems are ready for cell division.

Explanation:

Which of the following is the best summary for the function of the kidney?
A.
The kidney processes blood, sending nutrients to the bladder to be removed.
B.
The kidney filters the blood, keeping nutrients and getting rid of wastes.
C.
The kidney stores the blood so that it can use the nutrients.
D.
The kidney receives the used blood of the body and sends it all to the bladder.

Answers

Answer:

B.

Explanation:

GIVING BRAINLIEST> NEED HELP

Do the plants and animals on a farm make up an ecosystem? Why or why not?

Answers

Answer:

Yes they do

Explanation:

Animals and plants make up a ecosystem because the animals feed off the plants and the animals feed off of other animals.

Answer:

All living things in ecosystems are part of a food chain. A food chain is how energy is passed between things. farms are ecosystems. Farmers work with crops, animals, soils, and the weather to grow our food.

Explanation:

What is the main body system affected from sickle cell anemia?

Answer choices:

Digestive System

Nervous system

Circulatory System

Respiratory system

Muscular System

Skeletal System

Answers

Answer:

Any and all major organs are affected by sickle cell disease. The liver, heart, kidneys, gallbladder, eyes, bones, and joints can suffer damage from the abnormal function of the sickle cells and their inability to flow through the small blood vessels correctly.

Explanation:

Answer:

The main body system affected by sickle cell anemia is the Circulatory system.

Explanation:

According to John Hopkins Medicine (a reliable source), "Any and all major organs are affected by sickle cell disease. The liver, heart, kidneys, gallbladder, eyes, bones, and joints can suffer damage from the abnormal function of the sickle cells and their inability to flow through the small blood vessels correctly."

The __ is a series of geologic processes in which rock can form, change form one type to another, be destroyed, and form again.

Answers

Answer:

rock cycle

Explanation:

The nervous system and the muscle system responds to stimuli to produce motion. The movements of

Answers

Answer:

skeletal

Explanation:

skeletal bones are involuntary

Why would you expect to find a great deal of
parenchyma tissue in the roots of plants such as the
carrot, beet, and radish?

Answers

Answer:

Parenchyma tissue makes and stores food. Because the roots of carrots, beets, and radishes store food for plant, one would expect to find large amounts of parenchyma tissue in these parts.

ANSWER QUICKLY!!

Which of the following is correct?
A. The main difference between plants and animals is their method of nutrition and movement.
B. An animal which hibernating is not alive because it doesn’t eat of move.
C. All organisms are multicellular
D. All living things carry out six life processes.

Answers

Answer: A

Explanation:

 Because its the main difference between the 2

Answer:

its  A because the main difference bettween plants and animals is the cells its not B because animals dont die when they hibernate

its not C because viruses are are single sellular organisms

so that levaes D

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Explanation:

HELPPPPP OUT
PLZZZZZZ

Answers

Answer:

B. Single replacement reaction

Explanation:

A single displacement reaction is a chemical reaction in which one element is replaced by another in a compound.

Importance of using hand lens in classification of organisms​

Answers

Answer:

hand lens is used to make small object to appear larger hence visibility is easily attained

When it is summer in the U.S, why is it winter in South America?

Answers

Answer:

the suns on the other side of the earth at the time

Explanation:

hope it helps :)

Answer:

The Earth is tilted at an angle of about 23 degrees, which means that depending on where Earth is on its orbit, one pole will be getting more hours of light (summer) and one will be dark (winter).

Explanation:

Also, the earth is tilted on it's axis so more direct rays of sunlight hit the southern hemisphere making it summer in that hemisphere while it is winter in the northern hemisphere.  Six months later, this reverses, and more direct rays hit the northern hemisphere.  That's why it's summer here while it's winter there.  

The earth's distance from the sun has nothing to do with it.  It's all about the axial tilt.

Hope this helps!!

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What are herbaceous an is it a flower?

Answers

Answer:

Herbaceous plants are plants that have non-woody stems. (Like annuals and biennials).

why are there black holes on the moon

Answers

it’s caused by astroids hitting it
Other Questions
How do you know if a line is positive negative or undefined? An Environmental Wisdom worldview states that:Group of answer choicesOur success depends on how well we manage the earths life support system.The potential for economic growth is essentially unlimited.Because of our ingenuity, we will not run out of resources.Resources are limited and should not be wasted.We have an ethical responsibility to be caring managers to the earth. a seller of residential property in texas must give to the buyer of the property notice of the property's condition. which of the following is true? Mltiple representacin 1 2 Write the equation of each parabola given below. Vertex (-2, 5); focus (-2,-8) What is the importance of characteristics of light? in a time orientation, time is tangible and linear, whereas in a time orientation, time is approximate and cyclical. if the variance between treatments increases and the variance within treatments decreases, what will happen to the f-ratios and the likelihood of rejecting the null hypothesis in an anova test? Case study:Managing Inventory at Frito-LayFrito-Lay has flourished since its originthe 1931 purchase of a small San Antonio firm for $100 that included a recipe, 19 retail accounts, and a hand-operated potato ricer. The multi-billion-dollar company, headquartered in Dallas, now has 41 products15 with sales of over $100 million per year and 7 at over $1 billion in sales. Production takes place in 36 product-focused plants in the U.S. and Canada, with 48,000 employees.Inventory is a major investment and an expensive asset in most firms. Holding costs often exceed 25% of product value, but in Frito-Lays prepared food industry, holding cost can be much higher because the raw materials are perishable. In the food industry, inventory spoils. So poor inventory management is not only expensive but can also yield an unsatisfactory product that in the extreme can also ruin market acceptance.Major ingredients at Frito-Lay are corn meal, corn, potatoes, oil, and seasoning. Using potato chips to illustrate rapid inventory flow: potatoes are moved via truck from farm, to regional plants for processing, to warehouse, to the retail store. This happens in a matter of hoursnot days or weeks. This keeps freshness high and holding costs low.Frequent deliveries of the main ingredients at the Florida plant, for example, take several forms:Potatoes are delivered in 10 truckloads per day, with 150,000 lbs consumed in one shift: the entire potato storage area will only hold 7 hours worth of potatoes.Oil inventory arrives by rail car, which lasts only 4 days.Corn meal arrives from various farms in the Midwest, and inventory typically averages 4 days production.Seasoning inventory averages 7 days.Packaging inventory averages 8 to 10 days.Frito-Lays product-focused facility represents a major capital investment. That investment must achieve high utilization to be efficient. The capital cost must be spread over a substantial volume to drive down total cost of the snack foods produced. This demand for high utilization requires reliable equipment and tight schedules. Reliable machinery requires an inventory of critical components: this is known as MRO, or maintenance, repair, and operating supplies. MRO inventory of motors, switches, gears, bearings, and other critical specialized components can be costly but is necessary.Frito-Lays non-MRO inventory moves rapidly. Raw material quickly becomes work-in-process, moving through the system and out the door as a bag of chips in about shifts. Packaged finished products move from production to the distribution chain in less than 1.4 days.Questions:How does the mix of Frito-Lay inventory differ from those of a machine or cabinet shop (a process-focused facility)?In your opinion, why does inventory flow so quickly through a Frito-Lay plant?Explain FOUR (4) types of inventory available in Frito-Lay.Discuss FOUR (4) importance of forecasting for operation management functional area of an organization.Frito-Lay store in North Georgia stocks chips in its warehouse and sells it through an adjoining outlets. The store keeps several types of chip in stock, however its biggest seller is Super Ring Chip. The store wants to determine the optimal order and total inventory cost. Given the annual demand of 10, 000 boxes of Super Ring Chip, an annual carrying cost of $0.75/ pounds and ordering cost of $150. The Frito-Lay store would also like to know the number of orders that will be made annually and the time between orders. Given the store is open every day except Sunday and new year.The optimal order quantity per order.The minimum total annual inventory costs.he optimal number of orders per year.The optimal time between orders (in working days). What part of the problem-solving process involves thinking about what you would do differently next time?Question 3 options:Select the best solutionEvaluate the solutionExecute the solutionIdentify consequences What is the plot and setting of The Great Gatsby? What is chronological order format? 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