For the wild type (unmutated) enzyme, you measure a rate of p-nitrophenol release by the change in absorbance at 405 nm (for the slower phase only) of 0.216 (in a cuvette with a pathlength of 1 cm) over the course of two minutes. Use the Beer-Lambert Law to calculate the concentration of product released over this time; the extinction coefficient of p-nitrophenol at 405 nm is 18,000 M-1 cm-1.

Answers

Answer 1

Answer:

1.2x10⁻⁵M = Concentration of the product released

Explanation:

Lambert-Beer's law states the absorbance of a solution is directly proportional to its concentration. The equation is:

A = E*b*C

Where A is the absotbance of the solution: 0.216

E is the extinction coefficient = 18000M⁻¹cm⁻¹

b is patelength = 1cm

C is concentration of the solution

Replacing:

0.216 = 18000M⁻¹cm⁻¹*1cm*C

1.2x10⁻⁵M = Concentration of the product released

Related Questions

why was the introduction of oxygen such a big deal?

Answers

Its a big deal to learn about so you will know more about what you are breathing and what is going on in your body

1. Predict the missing product in the acid-base reaction.
HCL + NaOH
H2O +
HNa
CO2
Nach
О
CLOH

Answers

Answer: The missing product in the acid base reaction is NaCl.

Explanation:

Neutralization or acid base reaction is a chemical reaction in which acid and base combine to form salt and water. Acids donate [tex]H^+[/tex] ions and bases donate [tex]OH^-[/tex] ions  in water which combine to form water molecules.

The balanced chemical reaction for the given reaction is ;

[tex]HCl+NaOH\rightarrow NaCl+H_2O[/tex]

Thus the missing product is NaCl.

The missing product in the acid-base reaction is NaCl.

The balanced chemical reaction for the given reaction is ;

[tex]HCl +NaOH[/tex] → [tex]NaCl +H_20[/tex]

What is an acid-base reaction?

When acid and base react with each other they form salt and water. Water and salt both are neutral which means, whenever acid and base react together, they are neutralized by each other.

Neutralization or acid base reaction is a chemical reaction in which acid and base combine to form salt and water.

Acids donate  [tex]H^{+}[/tex] ions and bases donate  [tex]0H^{-}[/tex] ions in water which combine to form water molecules.

Since acid and base neutralize each other's effect,it is called neutralization reaction.

For Example: When Sodium Hydroxide ( NaOH) , a base, reacts with Hydrochloric acid (HCl) , it forms the salt, Sodium Chloride (NaCl) and Water.

The balanced chemical reaction for the given reaction is ;

[tex]HCl +NaOH[/tex] → [tex]NaCl +H_20[/tex]

Thus the missing product in the acid-base reaction is NaCl.

Learn more about the neutralization reaction here:

https://brainly.com/question/22945793

#SPJ5

Calculate the frequency of a light wave that has a wavelength of 9.2dm.

Answers

How I can do it ? I think u should multiply by formula

HNO3 + NaOH → NaNO3 + H2O

Answers

Answer:

Explanation: what is the question?

This is a neutralisation reaction.

Which of the following is a primary source for scientific information? O A. Doctoral theses OB. A handbook of scientific facts O C. Reviews of scientific studies D. Public opinion about a scientific issue​

Answers

D is the answer, Public op about a scientific issue

Examination of the first few successive ionization energies for a given element usually reveals a large jump between two ionization energies. For example, the successive ionization energies of magnesium show a large jump between IE2 and IE3. The successive ionization energies of aluminum show a large jump between IE3 and IE4. Explain why these jumps occurs and how you might predict them.

Answers

Answer:

See Explanation

Explanation:

Ionization energy refers to the energy required to remove an electron from an atom. Metals have lower ionization energy than non metals since ionization energy increases across a period.

One thing that we must have in mind is that it takes much more energy to remove an electron from an inner filled shell than it takes to remove an electron from an outermost incompletely filled shell.

Now let us consider the case of magnesium which has two outermost electrons. Between IE2 and IE3 we have now moved to an inner filled shell(IE3 refers to removal of electrons from the inner second shell) and a lot of energy is required to remove an electron from this inner filled shell, hence the jump.

For aluminium having three outermost electrons, there is a jump between IE3 and IE4 because IE4 deals with electron removal from a second inner filled shell and a lot of energy is involved in the process hence the jump.

Hence a jump occurs each time electrons are removed from an inner filled shell.

What volume (in mL) of 0.3900 M HCl is required to neutralize 50.00 mL of 0.8000 M NaOH?

Answers

Answer:

102.6 mL

Explanation:

The reaction that takes place is:

NaOH + HCl → NaCl + H₂O

First we calculate how many NaOH moles are there in 50.00 mL of a 0.8000 M solution:

0.8000 M * 50.00 mL = 40 mmol NaOH

Then we convert NaOH moles into HCl moles:

40 mmol NaOH * [tex]\frac{1mmolHCl}{1mmolNaOH}[/tex] = 40 mmol HCl

Finally we calculate the volume of a 0.3900 M solution would contain 40 milimoles:

40 mmol / 0.3900 M = 102.6 mL

Be sure to answer all parts. For an aqueous solution of sodium chloride (NaCl), determine the molarity of 4.05 L of a solution that contains 195 g of sodium chloride. M Determine the volume of this solution that would contain 4.63 moles of sodium chloride. L Determine the number of moles of sodium chloride in 25.35 L of this solution. mol

Answers

Answer:

[tex]0.824\ \text{M}[/tex]

[tex]5.62\ \text{L}[/tex]

[tex]20.89\ \text{mol}[/tex]

Explanation:

Molar mass of NaCl = 58.44 g/mol

Mass of NaCl = 195 g

Volume of solution = 4.05 L

Molarity of solution is

[tex]\dfrac{195}{4.05}\times \dfrac{1}{58.44}=0.824\ \text{M}[/tex]

The molarity of the solution is [tex]0.824\ \text{M}[/tex]

Moles of NaCl = 4.63 mol

Volume is given by

[tex]\dfrac{4.63}{0.824}=5.62\ \text{L}[/tex]

The volume of the solution that contains the required amount NaCl is [tex]5.62\ \text{L}[/tex]

Volume of solution = [tex]25.35\ \text{L}[/tex]

Moles of solution is given by

[tex]0.824\times 25.35=20.89\ \text{mol}[/tex]

The number of moles of NaCl is [tex]20.89\ \text{mol}[/tex].

What is the Kc for the following reaction at equilibrium at 800∘C if [CO2]= 0.030 M ? CaCO3(s)⇌CaO(s)+CO2(g)

Answers

Answer:

0.030

Explanation:

Step 1: Write the balanced reaction at equilibrium

CaCO₃(s) ⇌ CaO(s) + CO₂(g)

Step 2: Calculate the concentration equilibrium constant

The concentration equilibrium constant (Kc) is equal to the product of the concentrations of the products raised to their stoichiometric coefficients divided by the product of the concentrations of the reactants raised to their stoichiometric coefficients. It doesn't include solids or pure liquids.

Kc = [CO₂] = 0.030

2. A plant growing in response to the sunlight.
A.Gravitropism
B.Phototropism
C.Thigmotropism
D.Hydrotropism

Answers

Answer:

phototropism

Explanation:

since photo is (light?

Phototropism - because she photo means light hydro is water and gravi thing of earth

Ice at 0.0 degrees Celsius is combined with 50.0g of water at 75.0 degrees Celsius. Calculate the grams of ice present initially if the entire mixture comes to a final temperature of 25.00 degrees Celsius after the ice melts. Specific heat of water is 4.18 J/g degrees Celsius. SHOW WORK!

Answers

Answer:

m-Ice = 23.85g

Explanation:

The heat absorbed for the cold water (Heat of the increase of water + Heat of ice melting) is equal to the heat released for the water at 75°C. The equation is:

m-Ice-*C*ΔT + Hf*-m-Ice = m-water*C*ΔT

Where m-Ice- is our incognite

C is specific heat of water = 4.18J/g°C

ΔT is change in temperature = 25.0°C - 0.0°C = 25.0°C

Hf is enthalpy of fusion of water = 333.6J/g

m-water = 50.0g

ΔT is change in temperature = 75.0°C - 25.0°C = 50.0°C

m-Ice-*4.18J/g°C*25°C + 333.6J/g*-m-Ice = 50.0g*4.18J/g°C*50.0°C

104.5 m-Ice- + 333.6 m-Ice- = 10450

438.1 m-Ice = 10450

m-Ice = 23.85g

How many liters is 3.3 moles of a gas?

Answers

Answer:

224.1

Explanation:

A gas in a sealed container has a pressure of 125 atm at a temperature of 303 K. If the pressure in the container is increased to 200 atm, what is the new temperature if the volume remains constant?
(Show work pls :)!)

Answers

Answer:

485 K

Explanation:

Step 1: Given data

Initial pressure (P₁): 125 atmInitial temperature (T₁): 303 KFinal pressure (P₂): 200 atmFinal temperature (T₂): ?

Step 2: Calculate the final temperature of the gas

Assuming constant volume and ideal behavior, we can calculate the final temperature of the gas using Gay Lussac's law.

P₁/T₁ = P₂/T₂

T₂ = P₂ × T₁/P₁

T₂ = 200 atm × 303 K/125 atm = 485 K

To check the accuracy of a new method, a standard copper alloy containing 23.24% Cu was analyzed and calculated to contain 23.17%. calculate the relative error of the method in parts per thousand (ppt).

Answers

Answer:

3ppt is the relative error of the method

Explanation:

The relative error is a measurement used to determine the accuracy of your experiment, the equation to find the value in parts per thousand is:

Error = |True Value - Observed Value| / True Value * 1000

Where True value is 23.24%

And Observed value is 23.17%

Replacing:

Error = |23.24% - 23.17%| / 23.24% * 1000

Error =

3ppt is the relative error of the method

How many moles are in 454 Liters of a gas?

Answers

Answer:

20.27 mol

Explanation:

454 L x (1 mol/22.4 L) = 20.27 mol

A(n) _________ is an atom or a group of atoms with either a positive or negative charge
O compound
O ion
O molecule
O nucleus

Answers

Answer:

the answer is ion..

hope it helps..

The sender for this question would be ion.

After a metal object turns to dust (rust), it would have _____.
Need Help please

Answers

Answer:

The answer is high entropy.

Explanation:

Which process breaks down a compound into its elements?

Answers

Answer:

Chemical decomposition

Explanation:

Chemical decomposition, analysis or breakdown is the separation of a single chemical compound into its two or more elements or to simple compounds.

Answer:

Other processes that break down chemical substances into simpler substances, include depolymerization, decomposition, etc

Hope it helps!!!

Explanation:

Dawn is comparing how different animals move and the structures they use to move. She made the table shown below.


Animal Method of movement Structures used for movement
panther walks legs and feet
parrot flies wings
shark swims

Which structure completes the table for the shark?

eyes and brain

skin and scales

tail and fins

teeth and jaws

Answers

Answer:

tail and fins

Explatanation:

What type of consumer eats only producers?

Answers

Answer: Primary consumers make up the second trophic level. They are also called herbivores. They eat primary producers—plants or algae—and nothing else. For example, a grasshopper living in the Everglades is a primary consumer

Answer:

primary consumer make up the second trophic level .they are also herbivores they eat primary consumer plants or alger and nothing else .for example a grasshopper living in the everglades is a primary consumer

Question 6
Simplify the following expression
-3 + 4(a - 3b + 2) - 514 - a + 3b)​

Answers

This expression simplified is 3a-9b-509

select two correct answers

Answers

Answer:

D and E

Explanation:

The other answers don't support steel, they support iron or are supporting both, and the question is why steel alloys are more often used

C supports both

B supports how iron's strength

A supports iron being easily shaped and bent

hope this helps

Juan observes a material in the rocks of a hilluan hammers off a piece and then examines the pieces with a hana tena He
makes the following notes about what he observes

• has luster
• cubic crystals
• clear in color

What did he most likely observe through the hand lens?

A)
rock

B)
fossil
mineral

D)
animal bones

Answers

Answer:

I definitely think he mostly observed that it was clear in color.

Explanation:

If 4.50 moles of C2H6 undergoes combustion, how many moles of oxygen would it combust with?

Answers

Answer:

First balance the equation

C2H6 + 7/2O2 2CO2 +3H2O

for one mole of C2H6 there are 7/2 mole of O2 required. so for4.50 moles you require 4.50 x 7/2 = 15.75 moles of O2.

Explanation:

When Thomas Edison first sold electricity, he used zinc coulometers to measure charge consumption. (In a coulometer the cathode is weighed before a current is passed through it, and then again after the passage of current. The weight increase is proportional to the amount of current that flows through the cathode.) If the zinc plate in one of Edison's coulometers increased in mass by 14.47 g, how much charge had passed through it

Answers

Answer:

The answer is "[tex]42537.93 \ C[/tex]".

Explanation:

Molar mass : [tex]Z_n = 65.38 \ \frac{g}{mol}\\\\[/tex]

[tex]\to Zn^{2+} + 2e^{-} = Zn\\\\[/tex]

[tex]\therefore\\\\ \to 65.38 \ g \ of\ Z_n \ require: \\\\ \to 2 \times 96500\ C\\\\\to 14.41 \ g\ require,\\\\ \to \frac{(14.41 \times 2 \times 96500)}{65.38} \ \frac{g}{mol} = 42537.93 \ C[/tex]

What type of reproduction occurs in members of the bacteria kingdom?

Answers

Answer:

Bacteria can use sexual and asexual repoduction

Explanation:

What happens in a synthesis reaction?
A. Two substances combine to form a single new substance.
B. One substance breaks down into two substances.
C. One substance separates into ions in solution.
D. Atoms in two substances rearrange to form new substances.

Answers

Answer: A) two substances combine to form a single new substance

Answer:Two substances combine to form a single new substance.

Explanation: just took it

Which letter in the rock cycle diagram represents metamorphic rock?
A)
A.
B)
B.
)
D.
D)
E.

Answers

Answer:

B.

Explanation:

Hopes this helps. Mark as brainlest plz!

Answer:

b

Explanation:

learned this in science class

Question 9
5 pts
Which energy transfer is correctly matched?
Fusion in the sun: chemical to light and heat
Lighting a match: heat energy into chemical energy
a cell phone: thermal to electrical
Turning on a ceiling fan: electrical to mechanical

Answers

Answer:

fusion in the sun

Explanation:

I know this because I am currently talking this test and I know it cause I did it

Answer:Turning on a ceiling fan: electrical to mechanical

I took the test and Fusion in the sun: chemical to light and heat Lighting a match: sound energy into chemical energy were the wrong answers.

In many cases, a negatively charged compound (ex Cl-) will interact with a positively charged metal (ex Fe2 ). Usually we would consider this type of interaction to be ionic, but in the case of metals we do not. What type of bond is being formed when a ligand interacts with a metal and how could you know that the bond you are forming is that type of bond

Answers

Answer:

See Explanation

Explanation:

An ionic bond occurs due to electrostatic attraction between a positively charged ion and a negatively charged ion.

A metal and a ligand are bound by a coordinate covalent bond or a dative bond. This bond occurs due to donation of electron pairs from ligands to available orbitals on metals.

The formation of coordinate bonds is evident when neutral molecules or negative ions with non bonding electrons donate same to empty metal orbitals. This is sometimes shown by an arrow pointing from the ligands to the metal center.

For instance; tetraammine copper II ion is formed when four ammonia molecules donate a lone pair each to available vacant orbitals of the copper metal center to form [Cu(NH3)4]^2+.

Other Questions
pleaseeeeeeeeeeeeeeeeeee help PLEASE HELP URGENT!!! 30 POINTS TO WHOEVER ANSWERS BOTH QUESTIONS!! YOU DO NOT NEED TO SHOW YOUR WORKThe lengths of two sides of a triangle are given: 10 in and 3 in. What is the smallest possible whole number length for the third side? The lengths of two sides of a triangle are given: 10 in and 3 in. What is the largest possible whole number length for the third side? Consider the following ionization reaction.HNO3 (aq) + H20 () = NO,- (aq) + H2O* (aq)a)Define acid and base according to Bronsted-Lowry, and give an example of each.(3 marks)b) Identify all acids and its conjugate base pairs from the above equation.(2 marks) What is NOT a reason a new product might fail?O Too little marketing supportO Unexpected consumer reactionO Poor positioningO Appropriate channels selected Is 4x - 3x + 2 and x+2 equivalent? 3. What charge does the hydrogen atoms have in a molecule of water? alison earned $24 dollars by stocking shelves at grocery store for 3 hours Identify an example of personification (giving a non-human thing human-like qualities, "the rain danced on the roof"): explain what is being personified how. Find the AREA of a circle with RADIUS of 13 yards. What is the unit rate of 48 newspapers in 6 piles?________ newspapers per pile SOMEONE PLEASE HELP MEEE!!! An airplane is 16,000 feet above the ground. It begins to descend at a rate of 140 feet per minute. Write an equation you could use to solve for x, the number of minutes it will take until the plane is 12,600 feet above the ground. Is it more likely that the T. Rex would have a 3-chambered heart or a 4-chambered heart and why?Question 14 options:A 3-chambered heart because the caiman also has a 3-chambered heart and the T. Rex is most closely related to the caiman.A 3-chambered heart because the parrot has a 3-chambered heart and the parrot is most closely related to the T. Rex.A 4-chambered heart because the caiman also has a 4-chambered heart and the T. Rex is most closely related to the caiman.A 4-chambered heart because the parrot has a 4-chambered heart and the parrot is most closely related to the T. Rex. During the year, Walt who is self-employed travels from Seattle to Tokyo, Japan, on business. His time was spent as follows: two days travel (one day each way), two days business, and two days personal. His expenses for the trip were as follows (meals and lodging reflect only the business portion): Airfare $3,000 Lodging 2,000 Meals 1,000 Presuming no reimbursement, Walt's deductible expenses are: a.$3,500. b.$6,000. c.$4,500. d.$5,500. A bottle containing air is initially at a temperature of 33 degrees C and a pressure of 0.81 atm. After being placed in the freezer, the final temperature is 1 degrees C. What is the final pressure ? Le cinaste ________.A. dirige son quipe technique et les acteursB. manie la camra lors du tournageC. crit le scnario et les dialoguesD. fait l'enregistrement du son Which correctly forms verb tenses? Q: A: A) Clayton just got back from the doctor's. He is miserable and has been ill since Tuesday. If I had known that, I would bring him some chicken noodle soup. My mother has always said that chicken noodle soup can cure the worst cold. B) Clayton just got back from the doctor's. He is miserable and had been ill since Tuesday. If I had known that, I would of brought him some chicken noodle soup. My mother has always said that chicken noodle soup can cure the worst cold. C) Clayton just got back from the doctor's. He is miserable and has been ill since Tuesday. If I had knew that, I would have brought him some chicken noodle soup. My mother had always says that chicken noodle soup can cure the worst cold. D) Clayton just got back from the doctor's. He is miserable and has been ill since Tuesday. If I had known that, I would have brought him some chicken noodle soup. My mother has always said that chicken noodle soup can cure the worst cold. find the volume of the following square pyramid. geometry hw a man hikes 6.6 km north along a straight path with an average velocity of 4.2 km/h to the north. he rest at a bench for 15 min. then, he hikes 3.8 km birth with an average velocity of 5.1 km/h to the north. how long does it take the total hike to last Which activity can produce bugs that are harmful to the security of a Web server?