A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid
Answer:
tetracycline
Explanation:
When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while tetracycline gene is still intact.
- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.
- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.
- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.
Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.
Which phrase describes the purpose of ubiquitin
Answer:
regulates other proteins
Explanation:
Ubiquitin is a small protein that is found in almost all cellular tissues in humans and other eukaryotic organisms, which helps to regulate the processes of other proteins in the body.
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Which of the following fire-adapted blomes is dominated by shrubs with cool, wet winters and hot, dry summers?
•Chaparral
•Savanna
•Prairie
Answer:
I believe chaparral
Explanation:
1. Chromosomes are made of
and
Answer:
protein and DNA
Explanation:
Which biomes would contain animals that migrate?
a- savanna
b-tundra
c-temperate deciduous forest
d-all of the above
Answer:
the forest
Explanation:
select the correct statement about connective tissue. connective tissue cells are surrounded by a matrix made up of protein fibers and ground substances. correct or incorrect?
Answer:
correct
Explanation:
HURRY PLEASEEE. Photosynthesis and respiration are important because they produce or breakdown food to release energy. T F
Humans drill wells into Earth's crust to draw out groundwater for drinking and
irrigation of crops. Which spheres are interacting? *
O
Atmosphere, biosphere, and hydrosphere
O Biosphere, hydrosphere, and geosphere
Geosphere, hydrosphere, and atmosphere
Atmosphere, biosphere, geosphere, and hydrosphere
Answer:
Biosphere, geosphere, hydrosphere
Explanation:
Which elements are metals? Check all that apply.
Answer:
rhenium (Re), lithium (Li) , mercury (Hg)
Explanation:
hopes this helps u :)
how does nature show art
Answer:
"Nature in art can take many visual forms, from photorealism to abstraction. Art can mimic nature, by seeking to visually replicate objects as they actually appear in real life."
Explanation:
Photosynthesis and cellular respiration form direct ______ reactions.
Answer:
photosynthesis and cellular respiration form direct opposite reactions
Photosynthesis and cellular respiration form direct opposite reactions.Photosynthesis is the anabolic process and cellular respiration is the catabolic one.
What is the place for photosynthesis ?It takes place in the leaves where the chloroplasts have the cells that are undergoing the reactions where the food is synthesized.
Photosynthesis is the process by which plants prepare their food and it takes place in the presence of sunlight with the help of water and with the help of carbon dioxide it forms photosynthesis process where the reaction takes place.
Cellular respiration is the chain of reactions that are taking place in the cells where the reactions are taking place in the mitochondria and the chloroplasts and the reactions that are corresponding with each other.
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Urinary Journey
Name:
For your journey through the urinary system you must be made small enough to be filtered through the filtration
membrane from the bloodstream Into the renal You will be injected into the subclavian vein and must
pass through the heart before entering the arterial circulation. As you travel through the systemic circulation you have
a least 2 minutes to relax before reaching the ___(2).__artery, feeding the kidney. You see the kidney looming
brownish red through the artery wall. Once inside, the blood vessels of the kidney become increasingly smaller until
finally you reach the _3_ arteriole, feeding into the filtering device, or ____.Once in the filter, you
maneuver yourself so that you are directly in front of a pore. Within a fraction of a second, you are swept across the
filtration membrane into the (5)_ part of the nephron. Drifting along, you lower the specimen cup to gather
your first filtrate sample for testing. You study the readout from the sample and note it is very similar in composition to
_66) with one exception. There are essentially no ____. Your next sample doesn't have to be taken
until you reach the "hairpin" or using proper terminology the_8__. As you continue your journey, you notice
that the tubule cells have dense fingerlike projections extending from there surface into the lumen of the tubule.
These are which increase the absorptive surface area because this part of the tubule is very active in the
process of __(10)_ . While in the "hairpin" you collect your second sample. The readout verifies that the
concentration of the filtrate is much ___(11) and there are few nutrients such as ____(12)__and
(13)__.There is a much higher concentration of _(14) wastes here and the color is yellow, indicating
the presence of the pigment_(15)_ Gradually, you make your way up from the "hairpin and enter into an area
where large molecules are being moved into the filtrate you know you have arrived in the (16) and are
witnessing the process of_(12)_ You continue along and realize that the water level has dropped and the
stream have become more turbulent. You remember the role of the hormone_(18)__and think it must have
been released to conserve water in the body. You take an abrupt right and then drop straight downward into a tube
that has other tubes and fluid entering into it. You realize you are in the_(19)_ headed for the rinor calyx. You
enter into a much calmer are and flow very tranquilly Into a tube on the opposite shore. Upon entrance into this tube
you realize you are being squeezed rhythmically downward and you know you are in the _(20) _ Suddenly you
free-fall and splash into a large sea of urine, you know you have arrived at the _(21)
_There appears to be a lot
of fluld as the celling is getting closer and closer to you and suddenly the walls begin to gyrate and in a moment you are
being propelled out and into your hosts__ _(22) to exit the body.
Answer:for your journey through the urinary system you must be made small enough to be filtered through the filtration
membrane from the bloodstream Into the renal You will be injected into the subclavian vein and must
pass through the heart before entering the arterial circulation. As you travel through the systemic circulation you have
Explanation:
The urinary system, sometimes referred to as the renal system, manages fluid and electrolyte balance as well as filters and eliminates waste items from the body.
How does the urinary system work?You must be reduced in size to pass through the filtration membrane and into the renal system on your voyage via the urinary system. The subclavian vein will be used as the injection site, and you must first pass through the heart to reach the arterial circulation. You have at least two minutes to unwind as you move through the systemic circulation before you arrive at the renal artery, which supplies the kidney.
Through the artery wall, you can make out the towering, brownish-red kidney. Once within, the kidney's blood arteries get progressively smaller until you reach the afferent arteriole, which feeds into the glomerulus, the filtering organ. Once inside the filter, you position yourself such that you are facing a pore directly. You are quickly carried across the filtration membrane and into the Bowman's capsule region of the nephron. You lower the specimen cup as you proceed to collect your first test sample of filtrate.
You examine the reading from the sample and discover that, with one exception, its chemical makeup is very identical to that of blood. Practically no blood cells exist. You can wait to take your next sample until you get to the Henle loop, or the "hairpin" in correct language. You observe the tubule cells as you proceed along your path because they have numerous thick fingerlike projections that protrude into the tubule lumen from their surface.
This portion of the tubule is relatively small, therefore these microvilli improve the absorptive surface area. You gather your second sample while in the "hairpin". The readout confirms that the filtrate's content is significantly lower and that few nutrients, including glucose and amino acids, are present.
Here, metabolic wastes are substantially more concentrated, and the area is yellow, which denotes the presence of the urochrome pigment. You know you have arrived in the distal convoluted tubule and are seeing the process of secretion when you gradually work your way up from the 'hairpin' and enter into an area where big molecules are being transported into the filtrate.
As you proceed, you notice that the streams are now more agitated and the water level has plummeted. You recall the function of the hormone ADH and assume that the body must have secreted it to save water. You make a sharp right and then plunge straight down into a tube that is being filled with fluid and other tubes. You become aware that you are moving towards the minor calyx in the collecting duct.
You move very calmly into a tube on the other coast after entering a much calmer area. You become aware that you are experiencing a rhythmic downward squeeze as soon as you enter this tube, indicating that you are in the ureter. You know you have arrived to the urinary bladder when you suddenly drop and splash into a sizable sea of urine. As you can see, there is a lot of fluid as the ceiling gets closer to you and the walls start to pull. Suddenly, you are propelled out of the body and into your host's urethra to leave the body.
Therefore, the urinary system, sometimes referred to as the renal system, manages fluid and electrolyte balance as well as filters and eliminates waste items from the body.
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On average, an adult male sea otter weighs 36 kg (79 pounds), with some individuals weighing as much as 45 kg (99 pounds). A female weighs an average of 23 kg (50 pounds). To maintain their weight, otters must consume 250 kilocalories/kg of body weight each day to survive. Otters eat almost entirely small invertebrates for which they must dive to catch.
You have been tracking an adult female otter who weighs 25 kg. Answer the following questions based on the data provided.
How many kilocalories must she consume each day to maintain her body weight?
Question options:
6,250 Kcals
275 Kcals
1,620 Kcals
9,000 Kcals
Answer:
the answer that i would presume is a
Explanation:
25 x 250
The female otter which weighs 25 kg must consume a total of 6,250 kilocalories per day in order to maintain her body weight.
According to the illustration, otters must consume 250 kilocalories per kg of body weight in order to survive.
A female otter that weighs 25 kg must, therefore, eat 25x of the recommended kilocalories per kg daily in order to main her weight.
Thus: 25 x 250
= 6,250 kilocalories
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question 1. The construction of the Temple took seven years.
False True? question 2
Solomon sinned by allowing the corruption of God's people in the worship of idols.
True
False
Answer:
Question 1 true
question 2 true
Explanation:
Which component of a galaxy is colorful and found in the space between stars
Answer:
dust
Explanation:
cause dusk can be any colors
Space between stars:
In astronomy, the interstellar medium (ISM) is the matter and radiation that exist in the space between the star systems in a galaxy. This matter includes gas in ionic, atomic, and molecular form, as well as dust and cosmic rays. It fills interstellar space and blends smoothly into the surrounding intergalactic space.The component of a galaxy which is colorful is disk:
The various colors in a galaxy (red bulge, blue disks) is due to the types of stars found in those galaxy regions, called its stellar population.Learn more:
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pick two air masses and tell me about their characteristics include full name as well as the symbol
Answer:
.
Explanation:
Help, middle school science
Answer:
It's the second one because the rest are wrong
Answer:
They may be used to replace dead or damaged cells
Explanation:
when cells die or damage the body makes new cell to replace them and the process is called division
Which layer affects the oceanic and continental plates here on Earth?
Answer:
lithosphere
Explanation:
increase productivity due to nutrient abundance is called
Answer:
primary production-?
Explanation:
hope u get it right
What is a stimulus?
there are no options actually so plz help thank you
Answer:
In general, a stimulus is something that provokes or causes an action or response, as in Failing that test was the stimulus I needed to start studying harder. The plural of stimulus is stimuli. Its verb form is stimulate, which typically means to spur into action or to invigorate.
Explanation:
Answer:
In physiology, a stimulus is a detectable change in the physical or chemical structure of an organism's internal or external environment. The ability of an organism or organ to detect external stimuli, so that an appropriate reaction can be made, is called sensitivity
Explanation:
it just is
What part of the plant cell gives it is green color?
Answer:
Chloroplast
Explanation:
What actually makes plant green is a pigment called chlorophyll and it is found in the chloroplast
why is james charles gay
Answer:
cause nun of the girls in his school liked him
so he jump on the gay train
Explanation:
state 2 functions of mucus in small intestine
Answer:
Mucus is extremely important in the gastrointestinal tract. It forms an essential layer in the colon and in the small intestine that helps reduce intestinal inflammation by decreasing bacterial interaction with intestinal epithelial cells.
Explanation:
here are all of them
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A medical student is studying how the volume of blood in the circulatory system affects blood pressure. He measures the blood volume and pressure of two classmates, a man and a woman. He finds that the man has a higher blood volume but a lower blood pressure than the woman. He concludes that a person with low blood volume would tend to have high blood pressure. What additional factors should the student consider that would most likely affect blood pressure? (1 point)
Differences in overall outlook on life
Differences in size and gender
Differences in the day's food intake
Differences in the amount of recent exercise
Answer:
My guess is D, differences in the amount of recent exercise.
Is The Pamlico Sound a potable water source ? If so , Why ?
Answer:
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Explanation:
dkasnldkmasjkdjlkasnmlkdna∑∑∑∑∑Which statement explains one way that ponds and lakes differ?
A. ponds are shallower than lakes.
B. ponds are larger than lakes.
C. Pond water moves more than lake water.
D. lakes have more plants at the bottom than ponds.
Answer:
A. ponds are shallower than lakes
Explanation:
Ponds are not larger than lakes.
Pond water may move more than lakes in some situations, but it is not a defininte.
Lakes don't always have more vegetation than ponds.
Answer:
A. ponds are shallower than lakes.
Explanation:
Trust me on this, also can I have brainiest please?
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In Leonardo da Vinci’s time, nobody knew that the arteries were joined to the veins by tiny, invisible capillaries. This means that even though he studied the heart in detail, he thought that the arteries and veins were dead ends. Why did this make it difficult for him to understand how the heart worked?
Answer:
It was difficult for him to understand how the heart worked because he wasn't fully aware of the pumping mechanism of the heart and how the circulation of blood goes inside the body. Not seeing the capillaries meant that the heart pumped blood towards the vessels and into a dead end. He couldn't understand where the blood that was being pumped goes to.
Explanation:
Leonardo da Vinci is considered one of the earliest persons to study the heart. Although he made an exceptional observation of the different parts of the heart, he couldn't track the capillaries. He didn't know that these tiny structures connected the arteries and the veins. He believed that the heart was meant to transfer heat from the liver going to the muscles. He also believed that the lungs helped cool the blood.
How does nitrogen gas make its way into the atmosphere?
O animals eating plants
O decomposition
O using denitrifying bacteria
O cell respiration
Answer:
Explanation:
Using denitrifying bacteria is the correct answer
What best describes enzymes?
What is the correct order for the levels of organization and living systems from the simplest to the most complex
Answer:
organelle, cells, tissues, organs, organ systems, organisms, populations, communities, ecosystem, and biosphere
Explanation:
The biological levels of organization of living things arranged from the simplest to most complex