Dynamic cell seeding allows higher cell seeding efficacy to engineered 3D scaffolds therefore it is mostly preffered by the scientists. Fibroblasts tend to attach on the silicone tube wall but this stiuation is not appropriate for reching maximum cell seeding efficacy on a scaffold. Preventing cells for not to attach on silicone tube, applied force on a single fibroblast should exceed 6500 picoNewton (required minimum force). In the designed cell culture dynamic fibroblast seeding experiment, silicone tube inner diameter is 3.8 mm and fibroblasts surface area is 450 μm2. Please calculate the minimum flow rate as liter per minute of the fibroblasts included cell culture media through silicone tube to prevent fibroblasts adhesion on silicone tubes.

Answers

Answer 1

The minimum flow rate as liter per minute of the fibroblasts included cell culture media through silicone tube to prevent fibroblasts adhesion on silicone tubes is 769.210527 L/min.

What is the minimum flow rate about?

To calculate the minimum flow rate of the fibroblasts-included cell culture media through the silicone tube, we have to use the following equation:

Flow rate (L/min) = (Force (pN) * Surface area (μm^2)) / (Viscosity  * Inner diameter (mm))

In this case, the force applied on a single fibroblast should exceed 6500 picoNewtons, the surface area of the fibroblasts is 450 μm2, the viscosity of the cell culture media is typically around 0.5-1.5, and the inner diameter of the silicone tube is 3.8 mm. Plugging these values into the equation gives us:

Flow rate (L/min) = (6500 pN * 450 μm^2) / (1.0 * 3.8 mm)

= (2925000 pNμm^2) / (1.0 Pas * 3.8 mm)

= 769210.527 pNμm^2 / (Pas * mm)

= 769.210527 L/min

So the minimum flow rate of the fibroblasts-included cell culture media through the silicone tube to prevent fibroblast adhesion would be around 769.210527 L/min.

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Related Questions

(PLEASE HELP) Biodiversity affects the sustainability of an ecosystem by-
A. Always Increasing competition for limited resources within the ecosystem.
B. Allowing the ecosystem to survive many changes.
C. Preventing the ecosystem from surviving many changes.
D. Always decreasing competition for limited resources within the ecosystem.

Answers

C is the correct answer

Whal is the name for the negative subalomic particles in an atom?​

Answers

Answer:

Particles which are much smaller than atoms are known as subatomic particles. Subatomic particles are Neutrons, protons, and electrons

Explanation:

Answer:

a neutron..?

Explanation:

Which of the following is the correct classification for Dionaea muscipula?

Answers

Answer:

see the explanation and get the answer

Explanation:

Venus flytrap, (Dionaea muscipula), also called Venus's flytrap, perennial carnivorous plant of the sundew family (Droseraceae), notable for its unusual habit of catching and digesting insects and other small animals.

Which is not true about protein denaturation ?
a. can be induced by eat and extreme pH
b. Disturbs protein folding,
C. The primary structure of the protein rentains intaca hotarected by denaturation,
d. The protein remains functional after denaturation,​

Answers

D it is not functional
When a protein is denatured it becomes inactive. Therefore D will be our answer

Which type of macromolecules helps a cell brake down food?

Answers

Answer:

amylase, sucrase, lactase, or maltase.

Explanation:

this is because of proteins are always broken down by the the enzymes pepsin and peptidase, and by hydrochloric acid.

In animals, glucose is absorbed from the gut into intestinal cells against its concentration of gradient. This one-way process requires energy to work. Which of the following best describes how glucose is absorbed into intestinal cells?
A. By osmosis
B. By diffusion
C. By a buffer
D. By active transport

Answers

Answer: Glucose is absorbed through the intestine by a transepithelial transport system initiated at the apical membrane by the cotransporter

Explanation:

if im wrong ill give you your points

Osmosis best describes how glucose is absorbed into intestinal cells.

What do you mean by osmosis?

Osmosis is the movement of water molecules from a solution with a high concentration of water molecules to a solution with a lower concentration of water molecules, through a cell's partially permeable membrane.

Osmosis is when water moves from an area of LOW solute concentration (low osmolarity) to an area of HIGH solute concentration (high osmolarity) through a semipermeable membrane. Osmosis is one of the most important ways that plants and animals achieve homeostasis.

The absorption of water from the soil is due to osmosis. The plant roots have a higher concentration than the soil. Therefore, the water flows into the roots. The guard cells of the plants are also affected by osmosis.

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If the sugar has a carbonyl group within the carbon skeleton, we call it?
Glucose, Ketose ,Aldose,Galactose?

Answers

Answer:

Ketose

Explanation:

Monosaccharides also known as simple sugars are usually represented in a linear chain with a carbon skeleton. All the carbons present with the exception of one have a hydroxyl group that can be attached on either side of the skeleton.

Aldehyde sugars or aldoses such as glucose and galactose have a carbonyl group that is present at the end of the carbon skeleton. Monosaccharides also known as "ketone sugars", example fructose have their carbonyl group located at other parts of the carbon skeleton.

I need help with this, thank you

Answers

Answer:

a

Explanation:

the answer is a

universe is biggest and then Galaxy and solar system and then earth

the anwser is a! have a good day!

Proteins are:
a. Resistible to structural changes.
b. Polymers of amino acids bound by peptide bonds.
c. Have different structures but same function.
d. All hydrophilic​

Answers

Hello there!

Proteins are created by the bonding of amino acids. The bonds are called peptide bonds.

So the answer must be b.

I hope this helps!! :)

B. The polymers of amino acids are bounded by peptide bounds or polypeptides

Maria goes for a hike inside a cave. What types of rocks will she most likely see?

Answers

she will most likely see limestone

What are the similarities that all the organisms classified under the family Hominidae share?

Answers

Answer:

Among these adaptations are: streamlined bodies for efficient movement through water; forelimbs modified into flippers to aid in steering; hind limbs internalized remnants reducing drag; tail positioned horizontally to achieve a powerful up and down propulsion; hair replaced with under-skin blubber to provide warmth ...

Explanation:

In general, do organisms that have more traits in common also share more genes?

Answers

Answer:

In general, organisms that share similar physical features and genomes tend to be more closely related than those that do not. Such features that overlap both morphologically (in form) and genetically are referred to as homologous structures; they stem from developmental similarities that are based on evolution.

Explanation:

hope it helps!

The endocrine gland (s) referred to as the "master gland" is the:
A. pancreas
B. adrenal glands
C. thyroid gland
D. Pituitary gland

Answers

Answer:

pituilatry gland

Explanation:

The pituitary gland is sometimes called the "master" gland of the endocrine system because it controls the functions of many of the other endocrine glands. The pituitary gland is no larger than a pea, and is located at the base of the brain.

Describe your plan for how light intensity might affect the rate of photosynthesis? Help please I would really appreciate it

Answers

The closer the light is to the plant the rate of photosynthesis will be greater compared to when the light is further away from the plant the rate of photosynthesis will be lower.

Which is required for both anaerobic respiration and aerobic respiration?
oxygen
water
mitochondria
glucose

Answers

Answer:

oxygen is required for respiration and aerobic respiration

Both require glucose

Any mutation that affects just one base is called a -?- mutation.

Answers

Answer: A point mutation is when a single base pair is altered. Point mutations can have one of three effects. First, the base substitution can be a silent mutation where the altered codon corresponds to the same amino acid.

Explanation:

Why do tree's cells need to make more DNA?

Answers

It’s one of the big mysteries of cell biology. Why do mitochondria—the oval-shaped structures that power our cells—have their own DNA, and why have they kept it when the cell itself has plenty of its own genetic material? A new study may have found an answer.

It’s one of the big mysteries of cell biology. Why do mitochondria—the oval-shaped structures that power our cells—have their own DNA, and why have they kept it when the cell itself has plenty of its own genetic material? A new study may have found an answer.Scientists think that mitochondria were once independent single-celled organisms until, more than a billion years ago, they were swallowed by larger cells. Instead of being digested, they settled down and developed a mutually beneficial relationship developed

It’s one of the big mysteries of cell biology. Why do mitochondria—the oval-shaped structures that power our cells—have their own DNA, and why have they kept it when the cell itself has plenty of its own genetic material? A new study may have found an answer.Scientists think that mitochondria were once independent single-celled organisms until, more than a billion years ago, they were swallowed by larger cells. Instead of being digested, they settled down and developed a mutually beneficial relationship developed

I

Are floating cities the salutation to rising sea levels

Answers

Explanation: no sunken cities are


PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

Answer:D

Explanation:

Answer is D. Good luck. Hydropower plants capture the energy of falling water to generate electricity. Hydropower turbines kill and injure some of the fish that pass through the turbine.

When considering the entire period of time during which life on Earth has been evolving, we must look at:

thousands of years
hundreds of millions of years
hundreds of years
billions of years


millions of years

Answers

Answer:

we must look at billions of years

When considering the entire period of time during which life on Earth has been evolving, we must look at: D. billions of years.

Evolution can be defined as a process of gradual change in the characteristics of various species of living organism over several generations.

Basically, these characteristics refer to gene expressions that are typically transferred from parent organisms to their offspring or successive generations.

The earliest life forms known to humans existed about 3.7 billion years ago and they are microscopic organisms (microbes) whose fossils were found on rocks.

In conclusion, when you are considering the entire period of time during which life on Earth has been evolving, you must look at billions of years.

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What is the name of the bond that is created when two amino acids

Answers

Answer: peptide bonds

Explanation: I think that's the answer.

Nondisjunction occurs when chromosomes do not properly separate during cell division, which can lead to?.(please no links just written answers)

Group of answer choices

Duplications

Translocations

Aneuploidy

All the above

Answers

Answer:

Option C, Aneuploidy

Explanation:

Aneuploidy is defined as the condition when offspring/daughter cell has abnormal number of chromosomes.  

In the process of nondisjunction, the pair of homologous chromosomes do not separate and hence the sister chromatids fails to separate.

Hence, option C is correct

how does fertilizer affect the quality of crops?​

Answers

Answer:

Fertilizers replace the nutrients that crops remove from the soil. Without the addition of fertilizers, crop yields and agricultural productivity would be significantly reduced. That's why mineral fertilizers are used to supplement the soil's nutrient stocks with minerals that can be quickly absorbed and used by crops

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how does fertilizer affect the quality of crops?

[tex] \huge \mathsf \pink{Answer}[/tex]

The role of fertilizers in food production is usually underestimated. Fertilizers are food for plants.

Fertilizers replace the nutrients that crops remove from the soil. Without the addition of fertilizers, crop yields and agricultural productivity would be significantly reduced. That’s why mineral fertilizers are used to supplement the soil’s nutrient stocks with minerals that can be quickly absorbed and used by crops.

Put simply – we use fertilizer to:

Provide nutrients not available in the soil.Replace nutrients removed at harvest.Balance nutrients for better. producequality and higher yield.

Use the drop-down menus to choose the correct answer to complete each statement
An object that revolves around a planet is a
Jupiter is considered a
A large body in space that revolves around a star and does not give off light is a

Answers

Answer:
Moon, planet, planet
Explanation:
Moons revolve around planets, Jupiter is considered a planet because it revolves around the sun, and a planet is a body that revolves a star.

Answer:

Moon, planet and planet

Explanation:

What would happen if a mutation created a new ailele that decreased fitness?
a. The nen alle would spread through the population and decrease the foness of the population
ob. The new aliele would decrease the finess of the individual that possessed it and that individual would have few or no oftspring,
OC. The individual with the new ailele would wait for an environmental change so that is finess would increase again
od. The allele would be deleted by the bodys generic control mechanisms
Oe. The new ailele would have to change back to an allele of higher fitness so the possessor of that allele could maintain its fitness

Answers

Answer:

Hello There!!

Explanation:

The answer is b.the new allele would decrease the fitness of the individual that possessed it, and that individual would have few or no offspring.

hope this helps,have a great day!!

~Pinky~

A 23-year-old felt puffy, weak, and tired for several months. She suddenly noticed her urine had a red to brown discoloration and the volume was minimal. She went to the emergency room of a nearby hospital and the following data were obtained upon examination and testing what type of treatment does this person need?

Hematology:
Serum sodium 125 mEq/L
Serum potassium 6 mEq/L
Serum creatinine 2.6 mg/dL
BUN 24.0 mg/dL
pH (arterial) 7.32
Hematocrit 25%
Urinalysis:
Appearance Red to brown
Specific gravity 1.025
Blood Positive
Glucose Negative
Protein Mild
Renal Function Tests:
GFR (glomerular filtration rate) 40 mL/min
RBF (renal blood flow) 280 mL/min

Answers

Answer:

Renal failure treatment

Explanation:

The type of treatment this person requires is Renal failure treatment and this type of treatment will take the following steps :

Healthy eating and engaging in physical activity Controlled  Water intake in order to maintain a serum sodium value between 135- 145 mEq/ L . Sodium should be restricted or permitted, and this depends on the patients accumulation level of sodium  Angiotensin    Beta blocker and a diuretic

what organism performs only anaerobic respiration for energy
I need the answer to be five letters and as a hint, the third letter is a

Answers

Answer: Anaerobic cellular respiration

Some prokaryotes—bacteria and archaea—that live in low-oxygen environments rely on anaerobic respiration to break down fuels. For example, some archaea called methanogens can use carbon dioxide as a terminal electron acceptor, producing methane as a by-product.

Explanation: hopefully i helped

Which units are used in a pyramid of biomass?
A Individuals
B. g C/m2
C. m/s2
D. Klocalories

Answers

Answer:

g/m2 is the unit used in a pyramid of biomass. Kilocalories is Unit of measure for energy pyramids

Eutrophication refers to a process of change that takes place in a body of water. Which of the following causes eutrophication?
A)CFCs
B)acid rain
C)thermal pollution
D)excessive nutrients

Answers

The answer is D i believe. Hope this helps!!

Describe how the environment (predation, shelter, wind, access to water) might affect a population/ species?

Answers

The answer is environment is the us
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