DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment

Answers

Answer 1

one low-thickness and one middle-of-the-road thickness band. An organic chemist disconnects and decontaminates particles required for DNA replication.

coli cells become on a 15N medium are moved to a 14N medium and permitted to develop for two additional ages (two rounds of DNA replication). DNA extricated from these cells is centrifuged.

The processed DNA (5 to 10 kb; 10 to 50 mg/ml) was utilized to change skillful E. coli cells, however, no transformants were recuperated. Roundabout plasmids are significantly more productive for change than straight structures are (4).

Escherichia coli filling in a supplemented medium containing glucose don't use the milk sugar, or lactose (glucose-4-β-d-galactoside); be that as it may, on the off chance that the microorganisms are set in a development medium containing lactose as the sole wellspring of carbon, they combine β-galactosidase and can in this manner use lactose.

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Related Questions

What happens when protease is added to protein?

Answers

When protease is added, proteins breakes into smaller polypeptides or single amino acids, and forms new protein products.

A protease is an enzyme that catalyzes (increases reaction rate or "speeds up") proteolysis, breaking down proteins into smaller polypeptides or single amino acids and promoting the creation of new protein products. It is also known as a peptidase, proteinase, or proteolytic enzyme.

They achieve this by using hydrolysis, a mechanism in which water breaks bonds, to cleave the peptide bonds found in proteins. Protein digestion after ingestion, protein catabolism (the breakdown of aged proteins),[3][4] and cell signaling are only a few of the biological processes that proteases are involved in.

All living forms and viruses have proteases. They have repeatedly developed independently.

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What are Mendel's 4 hypotheses and what does each mean?

Answers

(1) Principle of Paired Factors is the first of Mendel's four postulates and principles of heredity. (2) The Law of Segregation or Law of the Purity of Gametes (Mendel's First Law of Inheritance), (3) The Dominance Principle, and (4) The Law of Independent Assortment

In his law of segregation, he put forth the concept of paired components being separated during the creation of gametes. According to Mendelian law, every pair of unit components would separately assort into brand-new gametes.

Only one version of a trait will emerge in the offspring of two parents who have different, opposing characteristics, according to Mendel's law of dominance. The dominant trait will be the only phenotypic trait that the hybrid offspring exhibits. The dominance law is the fundamental rule of inheritance.

Because a gamete only contains one of the two alleles dominant or recessive and not both, the law of segregation is also known as the law of pure gametes.

The development of reproductive cells causes various genes to separately separate from one another, according to the Principle of Independent Assortment. During his research on the genetics of pea plants in 1865, Gregor Mendel made the first observation of an independent assortment of genes and their related phenotypes.

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The Venn diagram compares aerobic and anaerobic respiration.
which statement could be categorized in the overlapping section of the Venn diagram?
A. uses pyruvic acid
B. has commercial uses
C. regenerates NADH
D. produces 36 ATP

Answers

The Venn diagram compares aerobic and anaerobic respiration. Uses of pyruvic acid could be categorized in the overlapping section of the Venn diagram.

In order to produce energy in the form of ATP molecules, aerobic respiration involves the complete breakdown of a glucose molecule in the presence of oxygen (36 molecules of ATP).

Contrarily, anaerobic respiration entails the partial breakdown of a glucose molecule to produce energy in the form of ATP molecules without the presence of oxygen (2 molecules of ATP).

The use of pyruvate is a characteristic shared by both aerobic and anaerobic respiration (pyruvic acid). The glycolytic process, which is the same for both processes, produces pyruvic acid.

While during anaerobic respiration, pyruvic acid is utilised to manufacture alcohol or lactic acid to renew NAD+, during aerobic respiration, pyruvic acid is used to build acetyl coenzyme A that enters the Krebs cycle.

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Answer:pyruvic acid is used

Explanation:i took it

What are the 5 characteristics of natural selection?

Answers

In fact 5 characteristics for any natural selection, is that it is so straightforward that it can be divided into five fundamental processes, which we'll refer to as VISTA in this instance: Variation, Inheritance, Selection, Time, and Adaptation.

Natural selection is a straightforward method that results in long-term population changes in living things. Three factors inevitably lead to natural selection: most qualities are inherited, more offspring are generated than can survive, and kids with more advantageous traits will endure and create more offspring than those with less advantageous traits.

Environment-adapted organisms have a higher chance of surviving and dispersing the genes that contributed to their success. Over time, this mechanism leads to the evolution and divergence of species. Natural selection is a method of accounting.

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Which two molecules bond together to form a disaccharide?

Answers

Answer:

Monosaccharides

Explanation:

Joining two Monosaccharides will create a Disaccharide. For example, the monosaccharides, glucose, and fructose bond together to form the disaccharide sucrose, which is produced by plants and is used in white sugar.

Label the following blood pressure readings as hypotensive, normal, pre-hypertensive, or hypertensive.

Answers

The following blood pressure readings are :

100/70 - Normal

150/95 - Hypertensive

130/85 - Pre-hypertensive

80/50 - Hypotensive

High blood pressure is nothing but high blood pressure and is a serious condition because it forces the heart to work harder to pump blood throughout the body and can increase the risk of heart attack, stroke and kidney failure if left untreated. Likewise, low blood pressure is hypotension.

Normal blood pressure varies from person to person, but the American Heart Association recommends a target blood pressure of less than 120 mm Hg systolic and less than 80 mm Hg diastolic. Stage 1 hypertension is when a person has a systolic blood pressure of 130-139 or a diastolic blood pressure of 80-89.

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Complete question :

Label the following blood pressure readings as hypotensive, normal, pre-hypertensive, or hypertensive.

100/70, 150/95, 130/85, 80/50

In your own words, describe what happens when a flat sign is put in front of a pitch and how the sound is affected.

Answers

Answer:

When a flat is put in front of a pitch it raises the pitch of the original note by a half step, a flat lowers the pitch of a note by one half step.

Explanation:

Cell volume (and therefore cell function) in most cells is dependent upon careful regulation of
A) permeability of cell membranes.
B) blood pressure.
C) volume of extracellular fluid.
D) resting membrane potential.
E) osmolarity of extracellular fluid.

Answers

In most cells, careful management of cell volume (and hence cell function)

How do cells form?

Through a process known as the cell cycle, new cells are generated from existing cells. A cell has the ability to duplicate itself and give rise to two additional daughter cells. Every cell cycle requires the performance of two key functions. Cells must first create a perfect replica of their DNA. membrane potential during rest.

Where do cells develop?

Depending on the area of the body, it can take a cell from the moment it is formed in the base layer of skin until it is lost from the surface for around a month.

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In most cells, careful management of cell volume (and hence cell function)

How do cells form?

Through a process known as the cell cycle, new cells are generated from existing cells. A cell has the ability to duplicate itself and give rise to two additional daughter cells. Every cell cycle requires the performance of two key functions. Cells must first create a perfect replica of their DNA. membrane potential during rest.

Where do cells develop?

Depending on the area of the body, it can take a cell from the moment it is formed in the base layer of skin until it is lost from the surface for around a month.

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you may have noticed that water sticks to surfaces such as glass,which property of water is responsible for this phenomena.

Answers

Answer: Adhesion

Explanation:

helix-turn-helix and zinc fingers are secondary structural motifs in dna and rna, respectively. true or false

Answers

In DNA and RNA, respectively, helix-turn-helix and zn fingers constitute secondary structural motifs. FALSE.

What is the main function of RNA?

Translation is the main mechanism by which RNA makes proteins. Genetic information is transmitted by RNA and decoded by ribosomes into a range of proteins needed for biological processes. rna, trna, and tRNA are the three main RNA varieties involved in the production of proteins.

What exactly is RNA, in simple terms?

Ribonucleic acid, sometimes known as RNA, is a nucleic acid that is present in all living cells and has characteristics similar to those of DNA. However, unlike DNA, RNA frequently has a single strand. The backbone of a Rna is composed of alternating hydrophilic groups and the sugar ribose, as opposed to the deoxyribose found in DNA.

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12. The structures labeled A, B, C, and D in the
diagram below represent
A-
(1) organelles
(2) organs
(3) nuclei
(4) mitochondria
BUD
B-
-C-
D-
(2
16. C
(1
17. N
(

Answers

Nuclei are labeled C in the diagram, as they are the larger structures and typically contain the genetic material (DNA) of the organism, while mitochondria are labeled D and are smaller organelles responsible for producing energy in the cell.

Energy from the cell is needed to move substances against a concentration difference.
Compare and Contrast What are the similarities and differences between facilitated
diffusion and active transport by a protein pump?

Answers

Facilitated diffusion and active transport are two processes by which cells can move substances across their membranes. Both processes involve the use of proteins to transport substances, and both require energy from the cell to function. However, there are also some key differences between the two processes:

Similarities:

Both facilitated diffusion and active transport involve the use of proteins to transport substances across a cell membrane.
Both processes require energy from the cell to function.
Differences:

Facilitated diffusion is a passive process, which means that it does not require the cell to use energy to actively pump substances across the membrane. Instead, substances are transported down their concentration gradient, from an area of high concentration to an area of low concentration.
Active transport, on the other hand, is an active process that requires the cell to use energy to pump substances against their concentration gradient, from an area of low concentration to an area of high concentration.
Facilitated diffusion can transport substances in either direction, while active transport can only transport substances in one direction (from low concentration to high concentration).
Facilitated diffusion is limited by the concentration gradient of the substance being transported, while active transport is not. This means that active transport can continue to transport substances even when their concentrations are equal on either side of the membrane.
Active transport is an active process

Is there a difference in diversity between the two sites if you looked at only the number of species

Answers

The difference in diversity of species which are present in two different sites cannot be determined just be looking at the number of species.

The diversity of species or species diversity can be defined as the number of different species which are present in an ecosystem and also the abundance of those species. Species diversity changes with change in location.

There are several factors which determine the species diversity. These factors include competition amongst the species, genetic diversity, diversity in habitat etc. These factors can help us determine the difference in species diversity of different locations. We cannot conclude regarding diversity just by looking at the number of species.

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Approximately 1.5 minutes of intense exercise will obtain ATP mostly from which process (think sprinting):
a. ATP
b. Anaerobic glycolysis
c. Phosphocreatine
d. Aerobic glycolysis

Answers

Approximately 1.5 minutes of intense exercise will obtain ATP mostly from the anaerobic glycolysis process.

Thus, the correct answer is B.

Аnаerobic glycolysis is the mаin metаbolic pаthwаy used in the setting of limited oxygen supply during exercise. It is used during high-intensity, sustаined, isometric muscle аctivity. It is inefficient from аn energetic stаndpoint аnd produces only two АTP molecules per glucose molecule, which is 19 times less thаn the full energy potentiаl of а glucose molecule. Despite its inefficiency, it is а rаpid process, аpproximаtely 100 times fаster thаn oxidаtive phosphorylаtion. The finаl step in the pаthwаy is the conversion of pyruvаte to lаctаte, which leаds to аccumulаtion of lаctic аcid.

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I need help with this. The topic is renewable and non renewable resources

Answers

Plastic packaging has non-renewable resources, whereas paper packaging is renewable. The difference between renewable and non-renewable energy sources is that renewables use resources that are not depleted or regenerated in nature.

What do you understand by non-renewable resources?

Non-renewable sources are those that have a limited reserve on our planet and that do not regenerate - or that take thousands of years to do so. Oil is perhaps the best known example, are ores and natural gas.

The difference between renewable and non-renewable energy sources is that renewables use resources that are not depleted or regenerated in nature. Non-renewable energy sources, on the other hand, use resources that are depleted in nature and, therefore, can generate several problems for the environment.

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HELP!!!!! How do musical embellishments help enhance a piece of music?

Answers

One of the few things that everyone can enjoy and relate to is music. We are all made of music.

What is Musical Embellishments?

It is an essential component of who we are. Since the very beginning of human history, music has existed in all civilizations and all countries. Music, according to Henry Wadsworth Longfellow, is the human race's global language.

Amazingly, music can unite disparate groups of individuals. We can learn how to relate to others and develop our empathy through music. Peace can be attained through music. Fear, separation, and misunderstanding obstacles can be destroyed with just one person's understanding.

In Northern Europe at the end of December 1914, during World War I, music effectively facilitated peace.

Therefore, One of the few things that everyone can enjoy and relate to is music. We are all made of music.

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What is the importance of mitosis for uni cellular organisms?

Answers

In unicellular organisms like bacteria, mitosis aids asexual reproduction by creating a near-exact duplicate of the parent cell.

A "amoeba" is another illustration of a eukaryotic, unicellular creature. An amoeba creates new life through cell division. Cell division in unicellular organisms is required just for reproduction. They divide their cells through a process called mitosis to create their daughter cells. Cell division is crucial for the development of new body cells and reproduction in multicellular animals. One pair of parents are used in asexual reproduction to create genetically identical kids. The primary method of cell division utilised by unicellular organisms is binary fission. The cell divides into two daughter cells that are genetically identical to one another.

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2. Baldness is a dominant trait. Tom's genotype for baldness is Hh. What describes Tom's genotype and phenotype?
homozygous; not bald
heterozygous; not bald
homozygous; bald
heterozygous; bald

Answers

He would be heterozygous; bald.

Based on Figure 1, which of the following statements best predicts the effect that a change from a moderately acidic environment (pH near 6) to a basic environment will have on peroxidase activity ?
O Peroxidase activity will decrease.
O Peroxidase activity will increase.
O Peroxidase activity will stay the same.
O Peroxidase activity will increase at first and then decrease.

Answers

The effect that a change from a moderately acidic environment (pH near 6) to a basic environment will have on O Peroxidase activity will decrease.

What is  Peroxidase activity?Demonstrated that the performance of hydrogen peroxidase was greatly affected by all three factors, temperature, concentration level, and pH.The peroxidases are enzymes whose primary function is to oxidize hydrogen donors at the expense of peroxides. They are highly specific for hydrogen peroxide, but they accept a wide range of hydrogen donors, including polyphenols.Thyroid peroxidase also called as thyroperoxidase (TPO) is mainly expressed in thyroid organs. It is a large transmembrane glycoprotein with covalently linked haem, present in cells on the apical membranePeroxidase activity was pH-dependent, with complete inactivation at pH 2.5 and when 8.5  The maximum enzyme activity observed at pH 6.5 decreased when the pH was reduced or increased; the drop was sharper in the alkaline than in the acid range

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T or F The study of orthologs would be useful to determine the function of a specific gene in a species.

Answers

Answer:

True

explaination non

I’m pretty sure the answer is true since it is useful to determine the function of a specific gene in species.

What data or test would you seek to determine whether or not a trait is sex linked? Select one: a. karyotype b. pedigree c. blood test d. DNA sequence

Answers

B) You would seek to determine whether a trait is sex-related using pedigree data or a test.

From a pedigree, how can you tell if a trait is sex-linked?

A sex-linked pedigree will primarily have affected males, and the female carriers will typically be marked with a half-shaded circle.

In a pedigree, how can you tell if a sex-linked trait is dominant or recessive?

The son must have only inherited his allele from his mother if the trait is x-linked, and the father may not be affected. RECEPTIVE WITH X-LINKS: The disease must be X-linked recessive when a affected non-founding son has two parents who are unaffected.

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Which of the following best describes the
general pattern of biochemical evidence for
evolution?

There is no general pattern observed in
biochemical evidence for evolution.

If two species have more differences in DNA,
RNA, and proteins, they are more closely
related.

The more closely related two species are, the
fewer biochemical similarities in DNA, RNA,
and proteins they have.

The more closely related two species are, the
more biochemical similarities in DNA, RNA, and
proteins they have.

Answers

Based on the fact that certain enzymes and chemical processes are found in the cells of all or nearly all life on Earth.

What exactly is biochemical evolution?

The changes that occur at the molecular level in organisms throughout time are referred to as biochemical evolution (molecular evolution). These vary from single nucleotide deletions, additions, or substitutions to the rearrangement of gene sections to the duplication of whole genes or even complete genomes.

Carl Neuber, a German scientist, created the term “biochemistry” in 1903. However, research into this very living part of chemistry had begun much earlier. Claude Bernard has been awarded the Sirehood of Biochemistry. Anatomy, molecular biology, biogeography, fossils, and direct observation are all evidence for evolution.

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This type of a boundary can be located at around 40°N latitude and 0° longitude?

Answers

3. Transform plate boundary can be located at around 40°N latitude and 0° longitude. It is a Venezuela region which lies in between South American Plate and Caribbean Plate.

What do plate tectonics entail?

Large rock slabs called tectonic plates that separate the Earth's crust move constantly, changing the topography of the planet. According to the system of ideas behind the plate tectonics theory, the lithosphere, which makes up Earth's outer shell, is divided into many plates that move across the rocky inner layer of the planet above its soft core (mantle).

What constitutes a plate boundary?

The line dividing two plates is referred to as a tectonic plate boundary. The tectonic plates are constantly and slowly shifting, but they do so in numerous directions. Some are travelling past each other while others are moving in opposite directions.

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What increases genetic variance?

Answers

The factors like genetic mutations, random mating, gene flow and genetic drift can increase genetic variance.

Genetic variations are the changes which occur in the genetic sequence of the DNA. These genetic variations are necessary as they allow the living beings to evolve and eventually survive. There are some factors which cause an increase in the genetic variation and they are genetic mutations, random mating, gene flow and genetic drift.

A genetic mutation is an alteration in the DNA sequence of a particular gene which causes a different product to form. This helps increase the genetic variance in organisms. Gene flow is defined as the movement of genes in and out of a particular population. In-flow of genes can help increase the genetic variation of individuals in that population. Genetic drift is the change in allelic frequency in a population by chance. Random mating is the mating of individuals that is not especially dictated by any social or genetic preference and leads to an increase in variation.

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Imagine you are stranded on a deserted island with 400 individuals. Once you and the others have built some shelters and taken stock of the island, how would you move forward

Answers

The question above seeks to assess your perception of government and your ability to manage a population. In that case, it's not correct for me to write an answer for you, but I'll show you how to create that answer.

Divide the 400 survivors into groups. Determine that each group is responsible for determining representatives, who will form the legislative body. Determine the number of representatives in each group according to the number of legislators you wish to have. You can assign 3-5 representatives for each group. You must divide these legislators into two chambers to maintain greater control of the laws that will be introduced. You can follow the example of the National Congress and research how it is established, to have a basis for the Congress you will form on the island.

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Complete question:

Imagine you are stranded on a deserted island with 400 individuals. Once you and the others have built some shelters and taken stock of the island, how would you move forward? Based on what you know about government, describe how you would set up the law-making body of government for the island’s survivors. Make sure to include the number of members in your legislature, how many houses it will have, how long members can be law makers, how they are chosen, and their responsibilities.

What is a characteristic displayed in an organism?

Answers

Answer: All living organisms share several key characteristics or functions: order, sensitivity or response to the environment, reproduction, growth and development, regulation, homeostasis, and energy processing.

For this assignment, you will investigate a timeline of warnings and decisions made during the Hurricane Katrina disaster. Then you will research hurricane forecasting and advanced warning systems and write a brief essay to report your findings

Answers

The timeline of warnings and decisions made during the Hurricane Katrina disaster begins on August 24, 2005 when the National Hurricane Center issued the first advisory on Hurricane Katrina. This advisory marked the beginning of the warning system and the start of the decision-making process. As the storm moved closer to the Gulf Coast, the warnings increased in intensity and the mandatory evacuation orders were issued. On August 29, 2005, the hurricane made landfall, causing widespread destruction and loss of life.

Essay Hurricane Forecasting and Advanced Warning Systems and write a short essay to report your findings.

In the aftermath of the disaster, it became clear that the advanced warning systems and decision-making processes used by government officials were inadequate. The National Hurricane Center had issued warnings and advisories weeks in advance, but these warnings were not heeded or acted upon. As a result, the impact of the storm was much more severe than it could have been.

To prevent similar disasters in the future, advanced warning systems and decision-making processes must be improved. One of the most important advances in hurricane forecasting is the use of satellite data to track storms and predict their paths. This data can be used to issue more accurate warnings and advisories so that the public can be better prepared for a hurricane’s arrival.

In conclusion, the timeline of warnings and decisions made during the Hurricane Katrina disaster reveals the inadequacies of the advanced warning systems and decision-making processes used by government officials. To prevent similar disasters in the future, improvements must be made to both the warning systems and the decision-making processes used by government officials.

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What general information do we need to research further to investigate the origin of life on Earth?

Answers

The fossil record is the information we need to research to investigate the origin of life on Earth.

How do we know the origin of life on Earth?

The earliest evidence of life on Earth comes from fossils that are discovered in Western Australia which is about 3. 5 billion years ago. These fossils are the structures called stromatolites which are formed by the growth of a layer of single-celled microbes such as cyanobacteria.

The major source to know the history of life on earth is fossil records. Fossils are the remains of organisms that are present centuries ago. They form when an organism dies and is burry in dirt and is solidified by minerals.

So we can conclude that fossils are the information that shows the life of the earth.

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Which location would energy be transferred more quickly between the atmosphere and Earth's surface, a lake where the air is 5*C or an ice sheet where the air is 5*C

Answers

I’m sorry I have no idea but please let me know when you’ve got the answer because I need it too

When trees lose leaves in the fall, they begin to regrow them again in the spring. Would you say this is an example of a process running in reverse or something else. Explain your reasoning.

Answer using at least 2 complete sentences.

Answers

Answer:

The process of trees losing their leaves in the fall and regrowing them in the spring is not an example of a process running in reverse. Rather, it is an example of a cyclical process, in which the tree goes through a period of dormancy during the winter and then becomes active again in the spring. This process is driven by changes in the length of daylight and temperature, as well as other factors such as the availability of water and nutrients.

During the fall, trees begin to prepare for winter by shedding their leaves, which helps them conserve energy and resources. In the spring, as the weather warms up and the days become longer, the trees begin to produce new leaves, which allow them to photosynthesize and produce the energy they need to grow and thrive.

So while it may seem like the process of leaf growth is running in reverse, it is actually a natural part of the tree's life cycle that happens every year.

The tree has a period of dormancy over the winter and then becomes active once again in the spring, which is an illustration of a cyclical process.

How do tree leaves regrow?

It is not a case of a process going backward when leaves fall off trees in the fall and grow again in the spring.

Trees lose their leaves in the fall to start preparing for the winter, which helps them save energy and resources.

The trees start to develop new leaves in the spring when the temperature increases and the days lengthen, allowing them to photosynthesize and generate the energy they require to grow and survive.

Therefore, the tree has a period of dormancy, when trees lose leaves in the fall, they begin to regrow them again in the spring is an illustration of a cyclical process.

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Other Questions
The Quaker Foods division of PepsiCo is most likely treated as a(n)A. revenue center.B. cost center.C. investment center.D. profit center. Write a program that displays a salary schedule, in tabular format, for teachers in a school district. The inputs are: Which of the following is the most likely reason that the greatest number of extinctions of bird species occurred in the period 1901-2000 What was similar about the colonization of New France and New Netherland? What was different? Write the Holiday Date of the following holidays:1. Bonifacio Day 2. Christmas Day 3. Rizal Day 4. Day of Valor 5. Labor Day 6. Independence Day 7. New Years Day 8. People Power Day 9. Ninoy Aquino Day 10. All Saints Day Which best describes the code of ethics? A B or C. Quickly pls :D.A. Professional standards of care are the only ones that subscribe to a code of ethics.B. Enforcing violations of the code of ethics can instill the publics trust in pharmacy professionals.C. Principles of the code include the profitability of working in the pharmaceutical business. Why is automation bad for the economy? According to categorical traits, which traits are shared between bipolar disorder, schizophrenia, and major depression Angie is working on solving the exponential equation 23x = 6; however, she is not quite sure where to start. Using complete sentences, describe to Angie how to solve this equation.Hint: Use the change of base formula: log base b of y equals log y over log b. 17. If most other species on Earth aren't monogamous, why are humans expected to be? How did teamwork allow you to learn more about the colonization efforts of Spain, France, and England The tendency of the media to make coverage and programming decisions based on what will attract a large audience and maximize profits is known as ______. What important, relevant aspect do the "run, hide, fight;" "add;" and "alice" protocols share? What is the message central message of the poem the daffodils justify your answer? A car moving at a constant speed passed a timing device at t=0. After 9 seconds the car has traveled 1,053ft. Write a linear function rule to model the distance in feet d the car has traveled any number of seconds t after passing the timing device. What are the benefits of health and wellness? Which detail from the text best supports the answer to part A How do you describe the mood of a story? Deon is running at a velocity of 5.4 meters per second. Deon has a total mass of 95 kilogramsincluding his helmet, uniform, and pads. If Deon runs directly into Chuck, who has a total mass of 120 kilograms and is standing still, what is the momentum of the Deon-Chuck system after the collision? Assume momentum is conserved. Wa the fall of Rome a major factor in the Eat-Wet Schim that occurred centurie later? Why or why not?In thi dicuion, take a tance on whether the fall of Rome eventually caued the Eat-Wet Schim, which led to the ditinction between the Catholic Church and the Eatern Orthodox Church. To formulate an opinion, review the hitory of Europe and the Near Eat between the reign of Diocletian in late antiquity and the chim itelf, which took place during the Middle Age. Support your opinion with clear, relevant example