"CRISPR" stands for Clustered Regularly Interspaced Short Palindromic Repeats, which are the hallmark of a bacterial defense system that forms the basis for CRISPR-Cas9 genome editing technology. In the field of genome engineering, the term "CRISPR" or "CRISPR-Cas9" is often used loosely to refer to the various CRISPR-Cas9 and -CPF1, (and other) systems that can be programmed to target specific stretches of genetic code and to edit DNA at precise locations, as well as for other purposes, such as for new diagnostic tools. How can this tool be used to alter genes in various organisms?

Answers

Answer 1

Answer:

by designing short guide RNAs (sgRNAs) customized to target genes of interest in the cells of these species

Explanation:

The CRISPR-Cas9 editing system is a versatile and powerful genome engineering tool for editing genomes, which can be directed to alter almost any DNA sequence in order to modify gene function. This system consists of an endonuclease protein (Cas 9) that cuts DNA at specific sites guided by a short guide RNA (sgRNA), which binds by base complementarity to the target sequence. This sgRNA must be designed with efficiency and specificity to target genes of interest. In consequence, the CRISPR-Cas9 genome editing system produces DNA double-strand breaks which may be repaired by 1- error-prone nonhomologous end joining (NHEJ) or 2-homology-directed repair (HDR) DNA repair pathways. According to the DNA repair pathway that has been activated, it is possible to trigger genetic modifications in the cells of different species (i.e., plant cells, animal cells, human cells, etc).


Related Questions

1) Dominant alleles are represented by a​

Answers

Dominant alleles are represented in an uppercase letter,

explain how darwin's journey to the galapagos islands contributed to his creation of the theory of evolutions.

Answers

Explanation:

During his visit to the islands, Darwin noted that the unique creatures were similar from island to island, but perfectly adapted to their environments which led him to ponder the origin of the islands' inhabitants.

Among those that struck Darwin so greatly were the finches that are now named in his honor. Darwin would later base some of his thought from the supposing that these finches were all descendents of the same lineage.

The eggs were placed in various solutions. What is the purpose of the vingear solution in this experiment​

Answers

Answer:

When the egg was in a solution (vinegar, salt water, tap water) that had a higher concentration of water than was inside the egg's membrane water flowed into the egg. When the egg was in a solution (corn syrup) where the concentration of water was less than inside its own membrane the water flowed out of the egg.

Answer:

Hi how are you doing today Jasmine

the variety of ecosystems in a given area is called

Answers

Answer:

ecosystem diversity. the variety of ecosystems within a given region.

Explanation:

The variety of ecosystems in a given area is called biodiversity, it is a variety of ecosystem species and genes in the world.

What is biodiversity?

The variety of plants, fungi, animals, and even microorganisms like bacteria that make up our natural world are all examples of the biodiversity that can be found in a given location.

To preserve equilibrium and support life, each of these species and organisms collaborate in ecosystems, much like a complex web.

The variety and variability of life on Earth is referred to as biodiversity or biological diversity. A measure of variation at the genetic, species, and ecosystem levels is called biodiversity.

Therefore, the variety of ecosystems in a given area is called biodiversity.

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1- why dong you usually get sick from the exact same pathogen twice

2-if a pathogen mutated so it had different antigens, would your memory B and memory T cells recognize it?

please help this is due at 3pm

Answers

1: For example, rhinovirus has over 100 different serotypes. Now, the good news is that you can't get ill from the same virus serotype right after getting better, due to the fact that you'll have developed antibodies which help to protect your immune system for a little while.

2:Most children in the United States are now vaccinated against measles virus; before vaccination was widespread, most were naturally exposed to this virus and suffered from an acute, unpleasant, and potentially dangerous viral illness. Whether through vaccination or infection, children exposed to the virus acquire long-term protection from measles. The same is true of many other acute infectious diseases: this state of protection is a consequence of immunological memory.

The basis of immunological memory has been hard to explore experimentally. Although the phenomenon was first recorded by the ancient Greeks and has been exploited routinely in vaccination programs for over 200 years, it is just now becoming clear that memory reflects a persistent population of specialized memory cells that is independent of the continued persistence of the original antigen that induced them. This mechanism of maintaining memory is consistent with the finding that only individuals who were themselves previously exposed to a given infectious agent are immune, and that memory is not dependent on repeated exposure to infection as a result of contacts with other infected individuals. This was established by observations made on remote island populations, where a virus such as measles can cause an epidemic, infecting all people living on the island at that time, after which the virus disappears for many years. On reintroduction from outside the island, the virus does not affect the original population but causes disease in those people born since the first epidemic. This means that immunological memory need not be maintained by repeated exposure to infectious virus

Read each of the answers below carefully: Which statement best describes the cell cycle? a. Cells grow and develop during interphase. Cells reproduce during the mitotic phase. b. Cells grow and develop during the mitotic phase. Cells reproduce during interphase. c. The nucleus of the cell divides during interphase. The cytoplasm of a cell divides during the mitotic phase. d. The nucleus of the cell divides during the mitotic phase. The cytoplasm of a cell divides during interphase.

Answers

Answer:

a. Cells grow and develop during interphase. Cells reproduce during the mitotic phase.

Explanation:

Cell cycle refers to the series of processes that leads to the growth/development and division of a cell. The cell cycle uses MITOSIS for cell growth. Mitosis comprises of two distinct stages namely: INTERPHASE AND MITOTIC PHASE. The interphase is referred to as the resting phase of the cell in which the cell grows and develops.

On the other hand, MITOTIC PHASE is the stage where the actual division of the nucleus (karyokinesis) and cytoplasm (cytokinesis) generally called CELL DIVISION occurs. Therefore, the cell reproduces i.e. one cell forming two, in the mitotic phase.

The statement best describes the cell cycle - a. Cells grow and develop during interphase. Cells reproduce during the mitotic phase.

Cell cyclethe series of processes that result in the growth and development and division of a cell.The cell cycle uses MITOSIS for cell growth. there are two distinct stages namely:INTERPHASE is referred to as the resting phase of the cell in which the cell grows and develops.MITOTIC PHASEis the stage where the actual division of the nucleus (karyokinesis)and division of cytoplasm (cytokinesis)

Thus, The statement best describes the cell cycle - a. Cells grow and develop during interphase. Cells reproduce during the mitotic phase.

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What might occur if humans reproduced through the process of mitosis?
A Many chromosomes would not be divided during cell division.
B Parents would produce more offspring.
C There would be the likelihood of an increase in genetic diversity.
D Inherited diseases would be passed down to all generations. HELPPPPP

Answers

Answer:

D

Explanation:

Mitosis creates genetically identical clones

Mitosis divides a cell into two identical daughter cells. Therefore, option (D) Inherited disease being passed down to all generations is correct.

What is mitosis?

Mitosis is the process of cell division that results in the production of new cells, which are then used for growth, repair, and the general replacement of older cells. A somatic cell will undergo this process when it has to reproduce and will end up with two new cells that are an exact copy of the first. The six steps of mitosis are completed by human somatic cells in between half an hour to one hours, depending on the type of tissue that is being duplicated.

Because there is little genetic variation as a result of mitosis, each generation is at risk for the same diseases and disorders. This is one of the drawbacks of the process.

Therefore, option (D) is correct.

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O False
Question 4 (1 point)
Organisms with jointed appendages are found in the Phylum Arthropoda.
O True
O False

Answers

true .................

Answer:

True

Explanation:

(Arthropoda means jointed appendages)

Fleas live on a cat and feed on the cat’s blood. The cat eats a bird outside in a rose garden. Which organism is a parasite in this scenario?

bird
cat
fleas
rose

Answers

Answer:

it fleas

Explanation:

because the cat just ate the bird and so more blood ( more blood for the flee ) so everytime the cat does this the flea will keep eating

Answer:

fleas

Explanation:

i got a 90% and  i got it right.

Need Help Due in 5 min: Earth Science

Answers:

hurricane


snowstorm


tornado


flooding

Answers

Snowstorm is the correct answer

Answer:  a snowstorm would occur

whats the difference between asexual and sexual reproduction???

Answers

Answer:

Sexual reproduction is the reproduction of 2 parents father and mother this is like most mammals,this is sexual. Asexual is the reproduction of 1 parent this is like many mushrooms and plants that include budding sporulation and others.

Copies of chromosomes are called

platelets.

sister chromatids.

macrophages.

stem cells.

Answers

copies of chromosomes are sister chromatids

What is one thing viruses all have in common?

A) Nucleus
B) Genetic Material
C) Cell Wall
D) Carbohydrate

Answers

Answer:

B) Genetic Material

Explanation:

Just did this lesson on Eg 2020

Hope this helps! Happy Holidays!

Question: Why do you deserve this scholarship? ...
Question: What are your career goals? ...
Question: Tell me about a mistake you made. ...
Question: Why did you choose this school? ...
Question: What activities are you involved in? ...
Question: Tell me about a personal achievement that makes you proud

Answers

Answer:

I deserve this scholarship because I work hard. I feel me getting this scholarship is my dedication paying off. My career goals are to be an orthopedic surgeon, so I can give back to my community, and help others. This has been my dream for a while. I chose this school because I feel it will help me better myself along the Journey of learning. I am involved in football, I was in the National Junior Honor Society in middle school, and I did some basketball. A personal achievement that makes me proud are all of the certificates on my walls of good grades and character badges.

 A scholarship is a form of financial support for students for further education. Scholarships are awarded based on various criteria such as academic merit, diversity and inclusion, athletic ability, and financial need.

 •  The award criteria typically reflect the values ​​and goals of the founder or sponsor of the award.

 • While grant recipients are not required to repay grants, the grants may require that the recipient continue to meet certain requirements throughout their support period, such as maintaining a minimum GPA or participating in a specific activity (e.g. recipient or as a teaching assistant for some graduate fellowships).

 • Scholarships may include a cash award, a non-cash award (such as a tuition or dormitory fee waiver), or a combination there of.

What is the purpose of scholarship for students?

 Scholarships are designed to reward a student's academic achievement and educational programs.Whether you are starting your career or studying in high school to acquire new skills, receiving a scholarship is a great accomplishment.

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what are the 10 central themes of biology and can you explain them?

Answers

Answer:

1. Emergent Properties

2. The Cell

3. Heritable Information

4. Structure and Function

5. Environmental Interactions

6. Feedback and Regulation

7. Unity and Diversity

8. Evolution

9. Inquiry

10. Science, Technology, and Society

Explanation:

1. The fact that complex organisms derive from small, simple bases.

2. Basic unit of life

3. Found in dna of all living organisms, passed from generation to generation.

4. All parts of organisms serve a purpose. (heart pumps blood)

5. All organisms are involved with their surroundings (plants use sunlight for energy

6. Bodies give us feedback on whether or not things are safe for habitation. (its too hot or cold)

7. All organisms may differ in looks but we are made up of similar DNA

8. explains how organisms develop over decades

9. scientists search for new information

10. We learn about the world through biology.

The different themes of biology include emergent properties, the cell, heritable information, structure and function, interaction with the environment, regulation, unity and diversity, evolution, scientific inquiry, and science, technology, and society.

What are the different themes of Biology?

A theme is an idea or a topic which is expanded in a discourse, discussion, etc. in literature, music, art, or any subject, discipline, etc.

The major themes of biology include:

Emergent Properties: These are critical attributes of biological systems, as the understanding of the individual parts alone is insufficient to understand or to predict the system behavior.

The Cell: It is the basic unit of life. Unit is capable of independent existence. Cells are studied to understand the functioning of organisms.

Heritable Information: This includes the transfer of information and genes from parents to offspring. This is included under genetics.

Structure/Function: This includes the structure and function study of living organisms.

Interaction with the Environment: This theme of biology includes the interaction of various living and non-living things with the environment.

Regulation: This theme involves the regulation of the biospheres of the planet.

Unity and Diversity

Evolution: This theme involves the evolution of different organisms over time which took place on this planet.

Scientific inquiry, Science, Technology, and Society

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Which sequence of human body structures is ordered from most complex to simplest?
O
A. heart cells, heart tissue, heart, circulatory system
B. circulatory system, heart, heart cells, heart tissue
o
C. circulatory system, heart, hobart tissue, heart cells
D. heart tissue, heart cells, heart, circulatory system

Answers

Answer:

A

Explanation:

Heart cells are complex to find

Heart tissue is somewhat easy to find

Heart circulatory system is easy to find

which molecule is a product of photosynthesis
A.Carbon dioxide
B.water
C.sugar
D.nitrogen

Answers

Answer:

I will say the answer is either A or B

Explanation:

the answer is A. Carbon dioxide

Look at the photo. Explain how it shows both an individual and a community

Answers

There are both living things ig

What is the function of the flower in plant reproduction?

disperses seeds


captures sunlight

protects the stem

attracts pollinators​

Answers

Answer:

attracts pollinators

How are proteins and nerve cells interrelated?​

Answers

Answer:

The answer to Your Question Is down below :)

Explanation:

Working with mice, scientists have discovered that a particular protein helps nerve cells extend themselves along the spinal cord during mammalian development.

It is not the number of different nucleotides, but the ________________ that is important in the ability of DNA to code for all the variability of organisms.

I'll mark brainlest

Answers

Answer:

discupa eu não sei

uma bela noite abençoe

The author suggests inertia is most often experienced
A. when planets orbit
B. in moving and stopping vehicles
C. at amusement parks
D. when watching rockets launch

Answers

Answer:

I think A when planets orbit

Which statement describes a cell part needed by the cell to make proteins?

Answers

Need the statements added to your question

Answer: The nucleus contains DNA.

Radio waves, infrared waves, visible light, and Xrays are all forms of energy
that travel as
a.mechanical waves
b.sound waves
c.electromagnetic waves
d.seismic waves

Answers

Answer:

the answer is c

Explanation:

Light is part of a spectrum of electromagnetic energy that includes radio waves, microwaves, infrared radiation, visible light, ultraviolet "light", x rays, and gamma rays. Electromagnetic energy travels as waves that vary in wavelength.

Which is the best description of the cause of sound waves?
a.a decrease in pressure
b.one object bumping into another
c.molecules becoming electrically charged
d. a vibration in material causing a vibration in an adjacent area

Answers

Answer:

the answer is d

Explanation:

When we hear something, we are sensing the vibrations in the air. The number of vibrations per second is known as the frequency, measured in Hertz (1 Hz = 1 vibration per second).

The Answer is D fs .....

Answer this pls!!!!!!!!!!!!

Answers

Answer:

sea anenome

Explanation:

It is sea anenomes because they provide shelter for sea creatures and they vary between hard and spft bodies

What are 3 functions of epithelium?

Answers

Answer:

Functions of epithelial cells include secretion, selective absorption, protection, transcellular transport, and sensing.

Explanation:

Epithelial layers contain no blood vessels, so they must receive nourishment via diffusion of substances from the underlying connective tissue, through the basement membrane.

A student collects 100 seeds from a group of plants produced from the
same parent plants. Of the seeds collected, 80 are blue seeds, and 20 are
white seeds. The trait for seed color is controlled by one gene and
results in seeds with either a blue color or white color.

Which Punnett square of the parent plants' traits likely corresponds
with the 100 seeds collected?

Answers

Answer:

A

Explanation:

What are the roles protein plays on living system? Explain it with 3 to 5 sentences ( THANK YOU BTW)​

Answers

Repair and Maintenance. Protein is termed the building block of the body.
Energy. Protein is a major source of energy.
Hormones. Protein is involved in the creation of some hormones.
Enzymes.
Transportation and Storage of Molecules.
Antibodies.

drag each label to the correct image identify the types of reproduction represented in the images

Answers

Tough one would have been expressing another way?
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