Correctly pair each of the temperatures used in the PCR reaction, with what occurs at that temperature. Annealing: 55 to 70 C
allow primers to anneal to the template

Extension: 65 to 72 C
allow DNA polymerase to build complementary DNA

Denaturation: 94 to 98 C
separate template DNA into single strands

Answers

Answer 1

Answer:65to72

Explanation:


Related Questions

How are humans classified within a food chain

Answers

Humans are classified within the food chain because we consume both plants and animals and they can occupy any of the 3 levels. They can be primary consumers when they eat plants, secondary consumers as well as tertiary consumers when they eat meat

How are humans classified within a food chain:

Humans are secondary consumers within the food chain

[ 2.2 on the Trophic level ]

Explanation:
They obtain their food from producers (plants) and other animals (herbivores and consumers)

cellulose is not highly branched because it ________.

Answers

Cellulose is not highly branched because it does not have alpha(1-->6) linkages.

Long chains of monosaccharides connected by glycosidic linkages are known as polysaccharides. Glucose makes up starch, glycogen, and cellulose, three significant polysaccharides. In plants and animals, respectively, starch and glycogen serve as temporary energy reserves. Glycosidic linkages bind together the glucose monomers.

A linear polymer glucan, cellulose is made up of over 100,000 glucose units connected by -beta (1-4)-glycosidic bonds. The cellulose chain's regular arrangement of hydroxyl groups causes the formation of H-bridges, which results in a fibrillar structure with crystalline properties.

Hydrogen bonds are used to bind the parallel-arranged cellulose molecules together. When combined with other cellulose molecules, these long, cable-like structures create the robust support structure.

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The polymerase chain reaction can only be used to amplify genes that have already been cloned and sequenced. Why is this true?.

Answers

This is True as the primers for the gene must first be obtained in order to abundantly segment a gene using PCR.

Genomic DNA (gDNA), complementary DNA (cDNA), and plasmid DNA are only a few examples of the types of DNA sources that may be used as PCR templates for replication. However, the DNA's structure or complexity influences the ideal input levels for PCR amplification. The sample is heated in order to cause the DNA to denature, or separate into two pieces of single-stranded DNA before the PCR is used to amplify a section of DNA.

Then, using the first two strands of DNA as templates, an enzyme called "Taq polymerase" creates or constructs two new strands of DNA. Instead of amplifying DNA using PCR, this method creates circles by cloning a collection of DNA fragments onto a retroviral vector.

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During adaptation to lower temperatures, an organism may increase its production of lipids with less saturated acyl chains.

a. True
b. False

Answers

It is wrong, because lipids are formed with more saturated acyl chains. During adaptation to low temperatures, organisms may increase the production of lipids with more saturated acyl chains. So it's false.

As temperature decreases, the composition of membrane lipids (phospholipid fatty acids) is expected to become more unsaturated to maintain homeostasis. Although they differ in function, storage lipids (triacylglycerol fatty acids) are expected to respond to temperature changes in a similar manner.

When saturated fatty acids are compressed by a drop in temperature, they are compressed together to form a dense and fairly hard membrane. When unsaturated fatty acids are compressed, their terminal 'kinks' displace adjacent phospholipid molecules, maintaining membrane fluidity.

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the diagram shows a beaker of water and molecules of black dye at the beginning of an experiment (time 1) and the same beaker of water and molecules of black dye after 30 mins (time 2)

a. b. molecules of water moved by osmosis from a lower solute concentration to a higher solute concentration
b. a, molecules of black dye moved by diffusion from a lower solute concentration to a higher solute concentration
c. d, molecules of water moved by osmosis from a higher solute concentration to a lower solute concentration
d. a, molecules of black dye by diffusion from a higher solute concentration to a lower solute concentration

Answers

Answer: D

Explanation:


What is the relationship between the cell size and the cell nutrient absorption rate?

Answers

The smaller the cell, the more favorable the ratio of volume to surface area for material exchange with the atmosphere is done by diffusion. Although surface area determines the rate at which nutrients are taken in or excreted, the volume determines how much enters and leaves the cell.


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Some researchers suggest that ______ molecules were the first information molecules formed on early earth.

Answers

Answer: RNA

Explanation:

The RNA world hypothesis suggests that life on Earth began with a simple RNA molecule that could copy itself.

The rate at which a specific allele for red flower color appears within a population is known as its.

Answers

The rate at which a specific allele for red flower color appears within a population is known as its  allele frequency .

Phenotype frequency: what is it?

Phenotype is the term used to describe how DNA manifests physically. The phenotypic ratio only describes the physical traits, or phenotypes, that are present in a population. The term "genotype" broadly refers to an organism's genetic make-up, or, to put it another way, the entire collection of its genes. The phrase can also be used more specifically to describe to a gene's alleles, or variant versions, that an organism carries.

The frequency of a particular allele in a population is known as the allele frequency (or gene frequency). The term "evolution" in population genetics refers to a shift in an allele's frequency within a population.

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what is the term that refers to the way the muscle fibers grow within the bundle?

Answers

The proper term would be the “grain”

A cell with 18 chromosomes undergoes mitosis. How many daughter cells are created? [a] each daughter cell has [b] chromosomes.

Answers

When a cell with prior 18 chromosomes undergoes mitosis, it has 2 daughter cells with 18 chromosomes later.

Mitosis is one of the two types of cell division in which the cell which undergoes this process is divided into two daughter cells which in turn have the same number of chromosomes as the parent cell.

Here, we had one parent cell which means that after mitosis it is going to be divided into two daughter cells. The parent cell has 18 chromosomes at first and since this is the process of mitosis, the daughter cells are going to have 18 chromosomes each after undergoing mitosis as well.

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If the size of an ecosystem decreases because of a hurricane, how are the organisms living in the ecosystem affected?
A. The carrying capacity of the ecosystem increases because there are more resources, decreasing competition among organisms.
B. The carrying capacity of the ecosystem decreases because there are more resources, decreasing competition among organisms.
C. The carrying capacity of the ecosystem decreases because there are fewer resources, increasing competition among organisms.
D. The carrying capacity of the ecosystem increases because there are fewer resources, decreasing competition among organisms.

Answers

If the size of an ecosystem decreases because of a hurricane, then the organisms living in the ecosystem are affected since the carrying capacity of the ecosystem decreases because there are fewer resources, increasing competition among organisms (Option C).

What is the carrying capacity of the ecosystem?

The carrying capacity of the ecosystem makes reference to the ability to support a given number of organisms and populations in a particular region.

Therefore, with this data, we can see that the carrying capacity depends on the ability to have resources in an ecosystem.

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What term is given to the projections of gray matter toward the outer surface of the spinal cord?.

Answers

The gray matter in the spinal cord is shaped like a butterfly. The dorsal process is called the dorsal horn and the ventral process is called the ventral horn.

What is a dorsal horn?

The dorsal horn of the spinal cord contains the first relays for afferent input from the skin, muscles, and internal organs of the body. The superficial dorsal horn contains at least some neurons that maintain selectivity for modalities encoded by primary afferent terminals. The intermediate processing center for this information is the dorsal horn, which consists of a complex network of excitatory, inhibitory, and projection neurons that transmit processed somatosensory information from the spinal cord to the brain.

At the back of the spinal cord, the central gray matter forms her two arms, each known as the dorsal horn. The dorsal horn consists of the cell bodies of sensory neurons. The front two arms of the spinal cord, located in the central gray matter, are called the anterior horns.

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A bacterial infection of the lining or valves of the heart is known as bacterial.

Answers

Inflammation of the inner lining of the heart's chambers and heart valves is known as endocarditis (endocardium). A bacterial infection is to blame.

A bacterial infection of the heart's inner layer or its heart valves is known as bacterial endocarditis. There are four heart valves. These valves facilitate the passage of blood from the heart and lungs to the rest of the body. These valves could malfunction in a person with bacterial endocarditis.

The heart may have to work harder, as a result, to pump blood to the body. The heart occasionally struggles to pump enough blood. A dangerous condition called bacterial endocarditis occasionally results in death.

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How have human impacted the ecosystem? ​

Answers

Answer:

Humans have impacted the ecosystem greater than any other organism on earth. Human life has done tremendous amount of damage. Humans have caused air pollution threw cars, buses/automobiles. The use of powerplants and production line seriously effects the eco system to a greater extent. I u have heard u leave a carbon footprint wherever you go which leaves a big impact on the ecoystem.

Explanation:

Giant tube worms living near vents on the ocean floor do not have a functioning intestine. Instead they have a modified gut full of bacteria that provide the worms with carbohydrates. What is the energy that the bacteria use to make the carbohydrates?.

Answers

The energy that bacteria use to make carbohydrates by consuming small bacteria that, through a process known as chemosynthesis, obtain all of their energy from the substances in the water, because of the dark hue of the material that these hydrothermal vents emit, they are also referred to as "black smokers."

Prior to the discovery of odd hydrothermal vents by scientists examining the deep Pacific Ocean floor, the enormous tube worm, also known as Riftia pachyptila, was completely unknown to science. These vents circulate water that seeps down through fractures or faults in the rock and is heated by volcanic activity. The water that comes out of the vent is incredibly chemical and mineral-rich. Scientists were startled to see entire ecosystems of species surviving near these vents because this deadly soup of chemicals would be fatal to the majority of animals.

These critters were prospering in total darkness despite the water's temperature being close to boiling. Because they do not rely on sunlight for energy, the species that dwell close to these vents are distinct from all other living beings on earth. Instead, the several smaller species of tube worms that live in shallower waters are closely linked to the big tube worms.

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Coral reefs look like they are made of rocks. They are actually made of animals. The animals are tiny.

Answers

Coral reefs are huge underwater structures composed of the skeletons of colonial marine invertebrates called coral.

The coral species that constructed reefs are known as hermatypic, or "hard," corals because they draw out calcium carbonate from seawater to produce a hard, durable exoskeleton that safeguards their soft, sac-like body.

Other species of corals that are not intricated in reef-building are known as soft corals. These types of corals are pliable organisms often similar to plants and trees and comprise species such as sea fans and sea whips.

Each coral is referred to as a polyp. Coral polyps thrive on the calcium carbonate exoskeletons of their ancestors, putting their exoskeleton to the existing structure.

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You are studying a drug that blocks translation in bacteria. You want to know more about its mechanism of action. You treat bacteria with the drug and isolate mrnas with their associated proteins from the treated bacteria. In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mrnas, but not the large subunits. In which stage does this drug arrest translation?.

Answers

According to the study, the drug arrest translation of proteins in the initiation stage. Thus, the correct option is A.

What is Translation?

Translation is the process of formation of proteins from the mRNA which is synthesized by the DNA in the nucleus of cell. Here, the drug which blocks translation in bacteria and the small ribosomal subunit (30S) binds to the mRNA but not the large ribosomal subunit (50S). So we can say that large ribosomal subunit binds to mRNA after the formation of the 30S initial complex. However, if it was treated with the drugs such as (clindamycin, macrolides, etc) in the translation process then this drug bind to the large ribosomal subunit (50S) and blocks the peptide bond formation between the amino acid and thus stop the initiation step of the translation.

So, we can say that if we use the drug in the bacterial translation process then the drug bind to the large ribosomal subunit and stop the initiation step of the translation.

Therefore, the correct option is A.

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Your question is incomplete, most probably the complete question is:

You are studying a drug that blocks translation in bacteria. You want to know more about its mechanism of action. You treat bacteria with the drug and isolate mrnas with their associated proteins from the treated bacteria. In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mrnas, but not the large subunits. In which stage does this drug arrest translation?

A. Initiation

B. Elongation

C. Termination


1. Which of the following is the main processing center for the entire nervous system?
a) central nervous system
b) somatic nervous system

Answers

The central nervous system

a research team used a culture of lymphocytes with radioactively labeled t nucleotides to study the cell cycle. they found that the lymphocytes incorporated the labeled nucleotide at a significantly higher rate after a pathogen was introduced into the culture. which of the following conclusions is most consistent with the results?

Answers

They concluded that infection causes lymphocytes to divide more rapidly.

A lymphocyte is a white blood cell type.  Cells of the blood White blood cells (monocytes, lymphocytes, neutrophils, eosinophils, basophils, and macrophages), red blood cells (erythrocytes), and platelets are all found in blood. The arteries and veins transport blood throughout the body. In adults, lymphocytosis is generally defined as a count of more than 3,000 lymphocytes per microliter of blood. The threshold for lymphocytosis in children varies with age. It can reach a maximum of 9,000 lymphocytes per microliter.

Vitamin C stimulates the production of B lymphocytes. Lymphocytes assist body's immune system in fighting cancer as well as foreign viruses and bacteria (antigens). Lymphocytes assist the immune system in remembering every antigen with which it comes into contact. Following an encounter, some lymphocytes transform into memory cells.

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how does the central nervous system integrate incoming information

Answers

Answer:

Explanation:

The brain and the spinal cord make up the central nervous system. The nervous system is the most complex and highly organized body system. It receives information from the sensory organs via nerves, transmits the information through the spinal cord, and processes it in the brain.

Via Nerves

Through the spinal cord and nerves, it carries information from the sensory organs to the brain, where it is processed.

Which of the following physical features would characterize an area that has low access to freshwater or limited rainfall?
O Dry
O Wet
O Cold
O Mountainous

Answers

Answer:  O Dry

Explanation:

Answer:

The answer would be dry.

Explanation:

It would be dry because, let’s say it was the desert… the dessert does not get much rainfall. Also it has barely any access to freshwater


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which of the following conditions is more common in women than in men
a. The internal pH of the lysosomes will decrease, which will prevent the activation of hydrolytic enzymes and interfere with the intracellular digestion of food
b. The internal pH of the lysosomes will increase, which will prevent the activation of hydrolytic enzymes and interfere with the intracellular digestion of food.
c. The internal pH of the lysosomes will decrease, which will activate hydrolytic enzymes and enhance the intracellular digestion of food.
d. The internal pH of the lysosomes will increase, which will activate hydrolytic enzymes and enhance the intracellular digestion of food.

Answers

Cystitis conditions is more common in women than in men.

The medical name for bladder inflammation is cystitis (sis-TIE-tis). An area of your body that is swollen and heated is said to be inflamed. It could also hurt.

The majority of the time, cystitis results from a bacterial infection. UTI is the medical term for this (UTI). A bladder infection can be uncomfortable and unpleasant. If the infection spreads to your kidneys, it might develop into a significant health issue.

Additionally, several medications or radiation treatments might cause cystitis. Cystitis can also be brought on by things that occasionally irritate the bladder, such as feminine products, spermicide jelly, or prolonged catheter use. Cystitis can also develop as a side effect of another condition.

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in bacteria, a partially diploid strain may result from . . . choose one or more:a.acquisition of an f' factor.b.natural transformation (not electroporation).c.acquisition of f factor.

Answers

In bacteria, a partially diploid strain may result from acquiring an F' factor and specialized transduction.

Specialized transduction and the acquisition of an F' factor lead to the emergence of a partly diploid strain in bacteria. Due to this, some of the bacteria's genetic components can exist in duplication (2 copies) (Chromosomes). One of those copies can be discovered on the bacteria's chromosome, while the other copy can be found in its plasmid.

Merodiploids are bacteria that have been found to have the partial diploid strain.

A genetic mechanism called specialized transduction allows genes to be transferred between bacteria. The F factor is also a plasmid that encourages bacteria to conjugate with one another and exchange genetic material.

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Though dolphins are more closely related to humans than they are to sharks, dolphins and sharks have both evolved bodies adapted to an aquatic environment. This is an example of.

Answers

Sharks and dolphins both have evolved bodies that are adapted to a water habitats, despite the fact that dolphins are more similar to humans than sharks are.

Why are people called humans, exactly?

The word "human" first appears in writing in the middle of the 13th century and derives from the Middle French humain, which means "of or belonging to man." That word itself derives from the Latin word humanus, which is supposed to be a cross between the words for "man" and "earth" (homo and humus). In contrast to birds, planes, and even celestial spirits, a human.

How does the Humans TV series end?

Niska makes the decision to go against her own, so Fred and the synths go without them. As her old owners leave to go home, Mia bids the Hawkins an emotional farewell. Once Karen has left, Pete meets her and they unwillingly depart together. Niska is shown in the closing scene reading one copy of the file while riding a train.

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Sharks and dolphins both have evolved bodies that are adapted to a water habitats, despite the fact that dolphins are more similar to humans than sharks are.

Why are people called humans, exactly?

The word "human" first appears in writing in the middle of the 13th century and derives from the Middle French humain, which means "of or belonging to man." That word itself derives from the Latin word humanus, which is supposed to be a cross between the words for "man" and "earth" (homo and humus). In contrast to birds, planes, and even celestial spirits, a human.

How does the Humans TV series end?

Niska makes the decision to go against her own, so Fred and the synths go without them. As her old owners leave to go home, Mia bids the Hawkins an emotional farewell. Once Karen has left, Pete meets her and they unwillingly depart together. Niska is shown in the closing scene reading one copy of the file while riding a train.

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functions can be called from statements in the body of a loop and loops can be called from within the body of a function.

Answers

Answer:  Functions can be called from statements in the body of a loop and loops can be called from within the body of a function. In a nested loop, the inner loop goes through all of its iterations for each iteration of the outer loop.

Explanation:

1. What is the color of the following plant pigments? which wavelengths do they absorb?.

Answers

The yellow-green pigment chlorophyll A absorbs light between the wavelengths of 430 and 662 nm.

How does chlorophyll affect the body?

Chlorophyll, according to supplement manufacturers, has a variety of benefits, including increasing red blood cells, aiding in weight loss, healing damaged skin, removing toxins, reducing inflammation, and preventing cancer. Although the list seems impressive, only a small portion of the claims are supported by research.

Why is green chlorophyll green?

The electromagnetic spectrum's blue and red halves are where chlorophyll absorbs light with the greatest intensity. The green color of tissues containing chlorophyll results from the fact that it is a poor absorber of the green and near-green regions of the spectrum.

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depressants are ________ of the gaba neurotransmitter, which has a quieting effect on the brain.

Answers

Depressants are agonists of the GABA neurotransmitter, which has a quieting effect on the brain.

Sedatives, tranquilizers, and hypnotics are examples of Central Nervous System (CNS) depressants. These medications have the ability to slow brain activity, making them useful for treating anxiety, panic attacks, acute stress reactions, and sleep disorders. Arousal and stimulation are reduced by depressant substances. They have an effect on the central nervous system, slowing messages between the brain and the body.

Gamma-aminobutyric acid (GABA) is an amino acid that serves as the central nervous system's primary inhibitory neurotransmitter. It works by inhibiting nerve transmission to reduce neuronal excitability. It slows down your brain by blocking specific central nervous system signals. GABA is known to have a calming effect.

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organisms that use co2 as their source of carbon are called multiple choice organotrophs. heterotrophs. autotrophs. chemotrophs.

Answers

Organisms that convert inorganic carbon dioxide (CO2) into organic carbon compounds are autotrophs.

Autotrophic organisms derive this essential element from carbon dioxide.

Mostly, Autotrophs are responsible for capturing the carbon dioxide from the air and use them in making organic compounds such as glucose. On the other hand  Heterotrophs, or the one who feeds on others for example humans, consume the organic molecules, and the organic carbon is passed through food chains and webs.

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explain transformation of force​

Answers

Answer:

THE TRANSFORMATION OF FORCE FROM A SYSTEM (S) WHERE A PARTICLE IS MOVING AT A SPEED (V) TO ANY SYSTEM (S) WHOCH IS MOVING RELATIVE SYSTEM. I HOPE THIS IS HELPFUL.

when the viral dna is incorporated into the host cells chromosome the viral dna is referred to as a

Answers

when the viral DNA  is incorporated into the host cells chromosome the viral DNA is referred to as a capsid

what is virus ?

A virus is a connecting link between living and nonliving organism, depend on living host cell for their survival, a chain of nucleic acids (DNA or RNA) which and use different parts of the cellular machinery to reproduce, and releases the replicated nucleic acid chains

A virus has a protein coat or protein envelope, which allows the virus to survive between hosts act as a protective covering, do not have cells and lack a cell wall .

it is surrounded by  protein coating called the capsid, genetic element present and is characterized by the evolution of the virus and the host.

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Cindy roller skates with a constantspeed of 18 km/h. How long will shetake to travel a distance of 54kilometres? Write a summary of what happens after hurricane. Include these terms in your summary: rebuilding, relief agencies, cleaning up, Caribbean . 8 to 10 please e e e e thanks Joaquin moved from nicaragua to the united states. He learned to speak and write in english, changed his last names so that it would sound more "american," and no longer maintains any of his old cultures styles of dress or customs. Joaquin has used which method of entering the majority?. The edges of a rectangular solid have these measures: 1. 5 feet by 1 feet by 3 inches. What is its volume in cubic inches?. Explain the meaning of Herberts primary imaginative comparison in The Windows, including a statement of the poems theme. Then point out several similarities and/or differences between Herberts meaning for light in his imaginative comparison and its meaning in The Nightingale and the Glowworm, The Souls Dark Cottage, and John 1. (20 points) A wholesale food supplier charges x dollars for each bag of flour and y dollars for each bag of sugar. Bakery A purchases seven bags of flour and five bags of sugar for a total cost of $260.45. Bakery B purchases five bags of flour and three bags of sugar for a total cost of $171.35. What is the cost of each bag of flour? Each bag of sugar? can anyone clarify what this is wich is the smallest angle in MNO? which graph represents y=-2x Divide. Write your answer on the simplest form.7/15 3/10 how did john hay convince other nations to accept the open-door policy which of the following best describes the rhetorical function of the second sentence in the passage? (a) it makes an appeal to authority. (b) it restates the thesis of the passage. (c) it expresses the causal relationship between morality and writing style. (d) it provides a specific example for the preceding generalization. (e) it presents a misconception that the author will correct. 5. About 0.36 of the students at a middle school are seventh graders. a. What fraction of the students at the middle school are not seventh graders? b.There are equal numbers of sixth and eighth graders at the middle school. What percent of the students at the middle school are sixth graders? Domino has always loved to draw. She sketches constantly and follows several artists she admires on social media. Her father suggests that she mightwant to look into a career in graphic design but Domino says that art is just a hobby and not a career. She reasons that it is not that hard and if she cando it, anyone can. What would be the BEST way for her father to respond to Domino?A.She is probably right-after all, everybody can draw.B.There are very few careers for artists so Domino has a good point.C.She should find a career that really challenges her and does not comes easy.D.What comes easily to you is a talent and is likely worth developing. given that integer array x has elements 5, 10, 15, 20, what is the output? What does Jurgis believe is his duty? (PLEASE HELP) you (mass 55 kg) are riding a frictionless skateboard (mass 5.0 kg) in a straight line at a speed of 4.5 m/s. a friend standing on a balcony above you drops a 2.5-kg sack of flour straight down into your arms. what is your new speed while you hold the sack? How did these documents influence the views ofthe American Colonists when they sought theirindependence from Great Britain? plsss help n which situations would a firm be most likely to use persuasive advertising? (choose every correct answer.) multiple select question. a brand that the firm wishes to reposition a product category in the growth or early maturity stage of the product life cycle a product category where competition is intense