A high GFP fluorescence elution fraction from a ni 2 agarose column will also have a high purity. This statement is false.
Several factors can contribute to the purity of an elution fraction, including the selectivity of the purification technique, the specificity of the affinity column, and the efficiency of the purification process. While Ni2+-agarose columns are commonly used for the purification of His-tagged proteins, they may still retain impurities or other proteins that have a weak affinity for the column.
To assess the purity of a protein sample, additional characterization techniques are typically employed. These may include SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) analysis, Western blotting, mass spectrometry, or other methods specific to the protein of interest.
Therefore, although a high GFP fluorescence in an elution fraction from a Ni2+-agarose column suggests the presence of GFP, it does not guarantee high purity. Additional characterization steps are necessary to assess the purity of the eluted protein sample accurately.
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Complete the complementary strand of DNA, using the same symbols for phosphates (circles), sugars (pentagons), and bases. Mark the 5' and 3' direction of the strands,
Drag the appropriate labels to their respective targets. Terms can be used once, more than once, or not at all
About the question:
You will find the completed strand in the attached files
Answer and Explanation:
First, we need to recognize each strand, the original one and the complementary one. We can recognize the original strand because bases are already given. So,
The original strand is the one at the left,The complementary strand is the one at the right.Now we need to recognize and pair the bases. Names are written with their letters.
Nitrogenated bases that form nucleic acids correspond to purines and pyrimidines. Adenine and guanine derive from purines, while Thymine and Citocine derive from Pyrimidines.
In the DNA molecule, Adenine (Purine) forms pairs with Timine (Pyrimidine), while Guanine (Purin) pairs with Cytosine. Two hydrogen bonds join the A-T pair, and three hydrogen bonds join the G-C.
Once we drag the base labels to their targets, we need to recognize each of the strain extremes.
One of the extremes has a phosphate group, which is named 5´. The other extreme has a hydroxyl OH named 3´. Both of them are readen in the 5´to 3´ direction. The polymerase enzyme reads the original strand in 5´ to 3´ mode, and the molecule lecture goes from 3´to 5´.
So, by looking at the labels, we will recognize the phosphate extreme and the hydroxyl one. We will place in the superior extreme of the original strain the phosphate group 5´ and in the other extreme the OH 3´. These positions will give us the direction reflected by the arrow. The complementary strand will have the same extreme labels but in opposite places. So first we will place the OH 3´ label in the superior extreme, and the phosphate 5´label in the inferior extreme, defining the direction reflected by the arrow.
1.2
A rose bush contains 1 000 kJ/m2 /year of energy and only 10% of this
energy is passed on at each trophic level of the food chain. How much
energy will be passed on to the blackbird? Show ALL your calculations
(4)
Answer:
What is the name of this website or the book?
An organism with a mutated cell
Answer:.
An organism with a mutated cell is mutant
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what is the potential disadvantage of external digestion
P S Q R The biological levels of organization range from a single organelle all the way up to the biosphere in a highly structured hierarchy. Multicellular organisms are organized from the simplest to most complex: cells, tissues, organs, organ systems, organisms. Evaluate the model above. Select ALL of the statements that accurately depict the examples shown in the model. A) R shows an animal cell. B) O shows types of tissue. P shows organs in the endocrine system. D) P shows an organ system, the digestive system. E) S shows an organ system, the digestive system.
Answer: the red thing pretend is blood and blue thing is water you first ta
Explanation:
Answer:
A) R → Q → P → S
Explanation:
I just took the test on USA Test Prep
FILL THE BLANK. 1. Nebula (cloud of gas and dust) Red Grant White Dwarf The above stages of evolution are for a Select one: O Low-mass star Ob. Normal-mass star OG High-mass star Od. Intermediate-mass star Through the use of a astronomers have determined that light from most stars is which produces evidence that the universe is ____ The statement above is completed correctly by the information in row Select one: O a radio telescope; blue-shifted; contracting. O b. radio telescope; red-shifted; expanding Oc. spectroscope; blue-shifted; contracting d. spectroscope; red-shifted; expanding Clear my choice
The above stages of evolution are for a high-mass star.
Through the use of a spectroscope, astronomers have determined that light from most stars is red-shifted, which produces evidence that the universe is expanding.
Explanation to the above given short answers are written below,
The stages of evolution mentioned in the question, namely nebula, red giant, and white dwarf, are characteristic of high-mass stars. Low-mass stars do not go through the red giant phase, and intermediate-mass stars have different evolutionary paths.
A spectroscope is an instrument used to analyze the light emitted or absorbed by celestial objects. When astronomers observe the light from most stars, they observe a redshift, meaning the light is shifted towards longer wavelengths.
This redshift is a result of the expansion of the universe, where galaxies and celestial objects are moving away from each other. The redshift provides evidence for the expanding universe and is a fundamental concept in cosmology.
Therefore, the correct completion of the statement is: Through the use of a spectroscope; red-shifted; expanding.
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Please help i am give brainilest
Which of the following characteristics is shared by viruses and living cells? Both viruses and living cells –
A. have a nucleus, as well as a cell wall.
B. contain nucleic acids such as DNA or RNA.
C. can replicate independently of a host cell.
D. use organelles to perform life functions.
i don't want to see any link
If i do you will be reported
Answer:
B
Explanation:
how do u get a perimeter of a rectangle?
Answer: All you have to do is add up all the sides
Explanation: Hope this helped you!!! Have a good day, and stay safe :)
Answer:
You would add all the sides
Explanation:
Area = multiplying 2 sides
Perimeter = adding up all the sides
so you would add all the sides of a rectangle
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Pls help me and thank youuuu
What's Chlorophyll ?
Answer:
Chlorophyll is any of several related green pigments found in the mesosomes of cyanobacteria and in the chloroplasts of algae and plants. Its name is derived from the Greek words χλωρός, khloros and φύλλον, phyllon. Chlorophyll is essential in photosynthesis, allowing plants to absorb energy from light.
Explanation:
Explanation:
Chlorophyll is a green photosynthetic pigment found in plants, algae, and cyanobacteria. Chlorophyll absorbs mostly in the blue and to a lesser extent red portions of the electromagnetic spectrum, hence its intense green color.
Which of the following amino acid changes can result from a single base change? a) Methionine to arginine. b) Histidine to glutamic acid. c) Glycine to alanine. d) Proline to alanine. e) Tyrosine to valine.
The amino acid change that can result from a single base change is : Glycine to alanine. (Option c)
A single base change in the DNA sequence, also known as a point mutation, can result in an amino acid change in the corresponding protein sequence. Among the given options, the amino acid change that can result from a single base change is:
c) Glycine to alanine.
Glycine (Gly) and alanine (Ala) are both nonpolar amino acids, and they have similar properties. They differ in the presence of a methyl group in alanine, which is absent in glycine. A single base change in the DNA sequence can lead to a substitution of one nucleotide, ultimately resulting in a change in the corresponding mRNA codon during translation. Depending on the specific codon affected, a glycine residue can be replaced by an alanine residue due to this single base change.
The other options:
a) Methionine (Met) to arginine (Arg): This change involves a substitution of a nonpolar amino acid with a positively charged amino acid. This change would typically require multiple nucleotide changes since methionine and arginine have different properties and are encoded by different codons.
b) Histidine (His) to glutamic acid (Glu): This change involves a substitution of a positively charged amino acid with a negatively charged amino acid. This change would typically require multiple nucleotide changes since histidine and glutamic acid have different properties and are encoded by different codons.
d) Proline (Pro) to alanine (Ala): This change involves a substitution of a cyclic amino acid (proline) with a nonpolar amino acid (alanine). This change would typically require multiple nucleotide changes since proline and alanine have different properties and are encoded by different codons.
e) Tyrosine (Tyr) to valine (Val): This change involves a substitution of an aromatic amino acid (tyrosine) with a nonpolar amino acid (valine). This change would typically require multiple nucleotide changes since tyrosine and valine have different properties and are encoded by different codons.
So, the only option where a single base change can lead to an amino acid change is c) Glycine to alanine.
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1. The sister chromatids are moving apart.
2. The nucleus and other organelles are no longer visible.
3. A new nuclear membrane is formed around the chromosomes.
4. The centrioles have reached the opposite poles of the cell.
5. The threadlike genetic material is formed and found in the nucleus.
6. The chromosomes are located at the equator of the cell.
7. The mitotic spindle disappears.
8. The centromeres of chromosomes split.
9. The mitotic spindle is formed.
10
11. The cell elongates.
12. Centrioles start to move toward the opposite poles of the cell.
13. Cytoplasmic contents start to duplicate.
14. The reverse of prophase.
15. The mitotic spindles converge and connect to the kinetochore of
chromosomes.
Please answer only if you know the answer I will give brainliest!
How does the movement, diet, and cellular structure of an organism play a role in classification?
Centrosomes and lysosomes are found in animal cells, but do not exist within plant cells.
The lysosomes are the animal cell’s “garbage disposal”, while in plant cells the same function takes place in vacuoles.
Plant cells have a cell wall, chloroplasts and other specialized plastids, and a large central vacuole, which are not found within animal cells.
The cell wall is a rigid covering that protects the cell, provides structural support, and gives shape to the cell.
The chloroplasts, found in plant cells, contain a green pigment called chlorophyll, which captures the light energy that drives the reactions of plant photosynthesis.
The central vacuole plays a key role in regulating a plant cell’s concentration of water in changing environmental conditions. follow me
Answer:
Describe the differences among meteoroids, meteors, and meteorites.
Explanation:
List the pros and cons of nuclear power, coal, natural gas, and
petroleum. Compare the lists and select the preferred source(s) of
energy and explain why
Nuclear Power Pros: Nuclear energy is also a cleaner energy source than many other types of power plants. It emits fewer greenhouse gases than other power plants, including fossil fuel plants.
Cons: The risk of a nuclear disaster is high and can cause severe environmental damage. Radiation leaks can have devastating impacts on local ecosystems, and nuclear waste is very challenging to dispose of safely. There is also a high cost associated with building a nuclear power plant
Nuclear Power Pros: Nuclear energy is a highly concentrated energy source. The power produced from one nuclear power plant can operate an entire city and more. It is also a cleaner energy source than many other types of power plants. It emits fewer greenhouse gases than other power plants, including fossil fuel plants. It also has a lower risk of air pollution than other sources. The supply of uranium is abundant and reliable for the next 100 years.
Cons: The risk of a nuclear disaster is high and can cause severe environmental damage. Radiation leaks can have devastating impacts on local ecosystems, and nuclear waste is very challenging to dispose of safely. There is also a high cost associated with building a nuclear power plant. The preferred source of energy is nuclear power. Nuclear energy is highly efficient, and the supply of uranium is reliable for the next century. Though the risk of a nuclear disaster is high, nuclear energy is still a cleaner source of energy and releases fewer greenhouse gases than other sources of power. The supply of uranium is also abundant and can provide power for a long time. Overall, nuclear energy should be viewed as a viable option to produce electricity.
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T/F. investigate two known causes for these rapidly dividing cells
True (T). Rapidly dividing cells have the ability to divide into two identical daughter cells more rapidly than normal cells.
This is because they are not subject to the same restrictions as normal cells when it comes to cellular division. Consequently, two known causes of rapidly dividing cells are mutations and stress. Mutations may lead to the formation of abnormal proteins or other cellular components that are unable to function correctly. Stress can also induce the overproduction of certain proteins or other cellular components.
Two known causes for rapidly dividing cells in cellular division are:
Cell Cycle Dysregulation: The cell cycle is a tightly regulated process that controls cell growth and division. Dysregulation of the cell cycle, such as the loss of cell cycle checkpoints or mutations in genes involved in cell cycle regulation, can lead to uncontrolled and rapid cell division. This dysregulation can result in the formation of tumors and is a hallmark of cancer.
Growth Factor Signaling: Growth factors are signaling molecules that stimulate cell growth and division. When cells are exposed to an excess of growth factors or when there is an abnormal activation of growth factor receptors, it can lead to uncontrolled cell division. Aberrant growth factor signaling pathways are implicated in various diseases, including cancer.
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assume that the unaided human eye has a limit of resolution of about 1 mm. what was the limit of resolution of hooke's microscope?
The limit of resolution of Hooke's microscope was significantly smaller than the unaided human eye.
Robert Hooke, an English scientist in the 17th century, is known for his work in microscopy. He developed an early compound microscope, which allowed for higher magnification and improved resolution compared to the unaided eye.
While the exact limit of resolution of Hooke's microscope is not provided, it is generally accepted that his microscope had a resolution on the order of micrometers (μm) or even smaller. This means that it could distinguish details and structures that were much smaller than what the unaided human eye could perceive.
The resolution of a microscope depends on various factors, including the quality of lenses, the wavelength of light used, and the numerical aperture of the system. Hooke's microscope likely had a resolution in the range of tens or hundreds of micrometers, enabling him to observe and document microscopic structures and organisms with greater detail and accuracy.
Hooke's microscope had a significantly higher limit of resolution than the unaided human eye, allowing him to explore and contribute to the field of microscopy and our understanding of the microscopic world.
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what is the difference between the way that saprotrophs and detritivores digest their food
Answer:
Externally
Explanation:
Usually, detritivores are mostly animals, while saprotrophs are mostly fungi. Furthermore, detritivores consume lumps of dead organic matter separately, while saprotrophs absorb chemically digested food. Saprotrophs digest their food externally, whereas detritivores do it internally in the digestive system.
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What kind of inheritance do you think the trait for the pedigree shows
Answer:
The information from a pedigree makes it possible to determine how certain alleles are inherited: whether they are dominant, recessive, autosomal, or sex-linked. To start reading a pedigree: Determine whether the trait is dominant or recessive. If the trait is dominant, one of the parents must have the trait.
Explanation:
When one DNA molecule is copied to make two DNA molecules, the new DNA contains 50% of the parent DNA. Why?
Answer:
When one DNA molecule is copied to make two DNA molecules, each new DNA contains 100% of the parent DNA. DNA replication uses each strand of a DNA molecule as a template for the creation of a new strand.
Explanation:
We should NEVER keep volatile solvents, like as ether, acetone, or benzene in an open beaker or Erlenmeyer flask because vapors can ignite and flash back if they reach a flame. All BUT ONE safety precaution is necessary when using volatile solvents. That is
"We should NEVER keep volatile solvents, like ether, acetone, or benzene in an open beaker or Erlenmeyer flask because vapors can ignite and flash back if they reach a flame.
All BUT ONE safety precaution is necessary when using volatile solvents."The necessary safety precaution when using volatile solvents that is not needed is: Using a volume of the solvent that is more than 100 mL.When using volatile solvents like ether, acetone, and benzene, the safety precautions that should be taken include the following:Wear proper PPE, such as safety glasses, gloves, and a lab coat.Keep the solvent container closed when it is not in use.Store solvents in designated flammable solvent cabinets.Keep volatile solvents away from any heat or open flames.Never heat volatile solvents with a Bunsen burner.
This can cause a fire.When handling volatile solvents, make sure there is proper ventilation in the laboratory. This can be achieved by using a fume hood.Use only small volumes of the solvent. If more than 100 mL is used, it can be dangerous to the user. Always ensure that the solvent is used in an area free of any ignition sources, including hot plates, Bunsen burners, and open flames.
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Why must everything but the independent
variable stay constant?
A. since a graph can only hold one variable
B. each group should be completely different to do an experiment
C. because you can only record information for one experimental
group
D. because with too many variables you can't determine what
caused the results
Other things but the independent variable must remain constant because; with too many variables you can't determine what caused the results.
What is independent variable?The independent variable is the variable that is changed in an experiment. Recall that every experimebt must have a dependent and independent variable.
Everything but the independent variable must stay constant because because with too many variables you can't determine what caused the results.
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All the organisms in a community, along with all interacting environment factors, form a (n) ________
A. population
B. community
C. large biome
D. ecosystem
Answer:
D ecosystem
Explanation:
A ecosystem is liveing spiecies interacting with thier enviornment together as a system.
short note of all disease of
Bee farming
Answer:
American Foulbrood is a highly lethal and contagious disease of honey bees, caused by the spore-forming bacteria Bacillus larvae. The disease causes larvae to die after cells are capped. Worker bees have difficulty trying to remove the dead larvae, and often contaminate the hive with additional spores.
Mention two ways in which plants are useful to animals and vice versa
Answer:
1.All animals depends on plants for their food and medicine
2.Plants gives animals oxygen which is essential for environment and animals for their survival.
Explanation:
It is difficult to achieve global sustainability because
A. it is an unrealistic goal.
B. governments do not always agree on how to solve environmental problems.
C. international agreements are not binding.
D. globalization is increasing.
Which components bond with andenine in a section if double stranded DNA
Answer: 3 and 5 only
Explanation:
Adenine is a purine nitrogenous base and it pairs with thymine which is a pyrimidine nitrogenous base with a triple hydrogen bond in a DNA structure. The adenine binds with thymine directly and indirectly with a deoxyribose sugar which is attached with it in the back it forms the part of sugar phosphate backbone and in the front hydrogen bonding helps in the stabilizing the DNA structure by binding two separate strands of DNA in a stable double helical structure.
The genetic code is carried ____ molecule in most organisms.
chromosomal
DNA
Guanine
hereditary
12. Define the differences between a crystalline and amorphous substance a. Relationship questions: 1. How does the bonding of atoms differ between an amorphous and crystalline substance. Draw a picture. 2. Explain why amorphous substances will not melt ---- they just get gooey" when heated 3. What is the relationship between a mineraloid and an amorphous substance 4. How does the term amorphous NOT relate to the mineral definition? 12. Define the differences between a crystalline and amorphous substance a. Relationship questions: 1. How does the bonding of atoms differ between an amorphous and crystalline substance. Draw a picture. 2. Explain why amorphous substances will not melt ---- they just get gooey" when heated 3. What is the relationship between a mineraloid and an amorphous substance 4. How does the term amorphous NOT relate to the mineral definition? 5. Identify 3 amorphous and 3 crystalline substances you have used.
1. In crystalline substances, the atoms are arranged in a highly ordered and repetitive pattern, forming a crystal lattice structure whereas amorphous substances lack a long-range order in their atomic arrangement.
2. Amorphous substances will not melt because they have no ordered pattern and will simply slide over each other.
3. Mineraloids are a subset of amorphous substances.
4. Amorphous substances are not classified as minerals because they lack the characteristic crystalline structure.
5. Three examples of amorphous substances are glass, rubber, and plastic.
What are amorphous and crystalline substances?Crystalline substances have a highly ordered and repeating three-dimensional arrangement of their constituent particles.
In a crystalline solid, the particles are arranged in a regular pattern, forming a crystal lattice.
Amorphous substances lack the long-range order and regularity of crystalline substances.
In an amorphous solid, the constituent particles are arranged in a disordered manner, without a repetitive pattern or crystal lattice structure.
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Why did the size of the caribou population decrease?
Answer:
The herds have been declining in recent decades due to a complicated mix of factors including hunting, disease, diminished food availability, and climate change, the report explains.
27. What will happen if one kidney of a person is removed?
A) He will survive and remain normal.
B)
He will die.
Urea will go on accumulating in the blood.
D)
Urination will stop.
E)
He will always feel thirsty.
Answer:
he will survive and remain normal
Answer:
Explanation:
Option A is the correct answer